Difference between revisions of "Team:HBUT-China/NoteBook"

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{{HBUT-China}}
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        #notebookapp{
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            font-family:"Times New Roman",Georgia,Serif
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        #notebookapp p{
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            font-size:16px;
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    </style>
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<div id="notebookapp">
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        <div style="width: 60%;margin:100px auto 0">
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            <div id="111">
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                <div id="date1" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.5</h1>
 +
                <h4>
 +
                    We synthesized:
 +
                </h4>
 +
                <h4 style="text-indent:28px">
 +
                    promoter TEF1 and terminator CYC1,&nbsp; hexa-His gene
 +
                </h4>
 +
                <h4 style="text-indent:28px">
 +
                    promoter GPM1 and terminator TDH1,&nbsp; nixA gene
 +
                </h4>
 +
                <h4 style="text-indent:28px">
 +
                    promoter TDH3 and terminator PGK1,&nbsp; TgMTP1t2 gene
 +
                </h4>
 +
                <h4 style="text-indent:28px">
 +
                    promoter TEF2 and terminator ENO2,&nbsp;&nbsp; kanR gene
 +
                </h4>
 +
                <h4>
 +
                    Each set of promoter terminators corresponds to a target gene.
 +
                </h4>
 +
                <el-collapse v-model="activeNames" @change="handleChange">
 +
                    <el-collapse-item name="11">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">pTEF1-tCYC1</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word;">
 +
                            GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCC</strong>CCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAA</strong>GGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC</strong>AGGCCTTACTAGTAGCGGCCG
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="12">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">pGPM1-tTDH1</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATA</strong>GGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACG</strong>AGGCCTTACTAGTAGCGGCCGCTGCAG
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="13">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">pTDH3-tPGK1</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAA</strong>GGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGT</strong>AGGCCTTACTAGTAGCGGCCGCTGCAG
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="14">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">pTEF2-tENO2</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAAC</strong>GGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAAT</strong>AAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCAG
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="15">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">hexa-His</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GGTCTCAATCTAAGTTTTAATTACAAAGGATCC<a>ATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAA</strong></a>GGATCCTCATGTAATTAGTTATGTCACGAGACC
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="16">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">NixA</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GGTCTCACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAA</strong>GGATCCATAAAGCAATCTTGATGAGGAGAGACC
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="17">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">TgMTP1t2</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GGTCTCAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAA</strong>GGATCCATTGAATTGAATTGAAATCGAGAGACC
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="18">
 +
                        <template slot="title">
 +
                            <p style="font-size:16px">KanR</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GGTCTCACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAA</strong>ATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTAGAGACC
 +
                        </p>
 +
                    </el-collapse-item>
 +
                </el-collapse>
 +
            </div>
 +
            <div id="222">
 +
                <div id="date2" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.6.1 ~ 6.10</h1>
 +
                <h4>Construction plasmids：</h4>
 +
                <el-collapse v-model="activeNames" @change="handleChange">
 +
                    <el-collapse-item name="21">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126001 (pSB1C3-pTEF1/tCYC1)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126001</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word;">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="22">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126002 (pSB1C3-pGPM1/tTDH1)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126002</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="23">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126003 (pSB1C3-pTDH3/tPGK1)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126003</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                </el-collapse>
 +
                <p style="word-wrap:break-word">
 +
                    We insert promoter TEF1 and terminator CYC1, promoter GPM1 and terminator TDH1, promoter TDH3 and
 +
                    terminator PGK1, promoter TEF2 and terminator ENO2 into vector pSB1C3 using restriction
 +
                    endonuclease and T4 DNA ligase, respectively.
 +
                </p>
 +
                <p>
 +
                    The pSB1C3 digestion system we used:
 +
                </p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    pSB1C3
 +
                                </td>
 +
                                <td width="300">
 +
                                    15 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    <em>EcoR</em>I
 +
                                </td>
 +
                                <td width="300">
 +
                                    3 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    <em>Pst</em>I
 +
                                </td>
 +
                                <td width="300">
 +
                                    3 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    Cutsmart Buffer
 +
                                </td>
 +
                                <td width="300">
 +
                                    5 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    ddH<sub>2</sub>O
 +
                                </td>
 +
                                <td width="300">
 +
                                    24 μL
 +
                                </td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <p>
 +
                    <br />
 +
                </p>
 +
                <p>The enzyme system we use:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    pSB1C3
 +
                                </td>
 +
                                <td width="300">
 +
                                    2 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    fragment
 +
                                </td>
 +
                                <td width="300">
 +
                                    6 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    T4 DNA Ligase
 +
                                </td>
 +
                                <td width="300">
 +
                                    0.5 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    T4 Buffer
 +
                                </td>
 +
                                <td width="300">
 +
                                    1 μL
 +
                                </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">
 +
                                    ddH2O
 +
                                </td>
 +
                                <td width="300">
 +
                                    0.5 μL
 +
                                </td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <div>
 +
                    <p>BBa_k3126001:</p>
 +
                    <div style="text-align:center">
 +
                        <img src="https://2019.igem.org/wiki/images/0/06/T--HBUT-China--_BBa-k3126001.png" width="400">
 +
                    </div>
 +
                    <p>BBa_k3126002:</p>
 +
                    <div style="text-align:center">
 +
                    <img src="https://2019.igem.org/wiki/images/8/8b/T--HBUT-China--photoBBa-k3126002.png" width="400">
 +
                    </div>
 +
                    <p>BBa_k3126003:</p>
 +
                   
 +
                    <div style="text-align:center">
 +
                    <img src="https://2019.igem.org/wiki/images/c/cf/T--HBUT-China--photoBBa-k3126003.png" width="400">
 +
                    </div>
 +
 
 +
                    <p>BBa_k3126004:</p>
 +
                    <div style="text-align:center">
 +
                    <img src="https://2019.igem.org/wiki/images/4/48/T--HBUT-China--photoBBa-k3126004.png" width="400">
 +
                    </div>
 +
                    <div>
 +
                        <img src="https://2019.igem.org/wiki/images/a/a8/T--HBUT-China--photoPlasmid--figure--BBa-k3126001.png" width="49%">
 +
                        <img src="https://2019.igem.org/wiki/images/4/43/T--HBUT-China--photoPlasmid--figure--BBa-k3126002.png" width="49%">
 +
                    </div>
 +
                    <div>
 +
                        <img src="https://2019.igem.org/wiki/images/8/88/T--HBUT-China--photoPlasmid--figure--BBa-k3126003.png" width="49%">
 +
                        <img src="https://2019.igem.org/wiki/images/4/48/T--HBUT-China--photoPlasmid--figure--BBa-k3126004.png" width="49%">   
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <div id="333">
 +
                <div id="date3" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.6.10 ~ 6.20</h1>
 +
                <h4>Construction plasmids：</h4>
 +
                <el-collapse v-model="activeNames" @change="handleChange">
 +
                    <el-collapse-item name="31">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126005 (pSB1C3-pTEF1/ tCYC1/ hexa-His)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126005</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word;">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCC<strong>ATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAA</strong>GGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="32">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126006 (pSB1C3-pGPM1/tTDH1/ nixA)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126006</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCC<strong>ATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAA</strong>GGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="33">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126007 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126007</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCC<strong>ATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAA</strong>GGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="34">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126008 (pSB1C3-pTEF2/tENO2/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126008</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCC<strong>ATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAA</strong>GGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                </el-collapse>
 +
                <p style="word-wrap:break-word">
 +
                  Insertion of gene into plasmid carrying promoter and terminator. 
 +
                </p>
 +
                <p>BBa_K3126005 (pSB1C3-pTEF1/ tCYC1/ hexa His):</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126001</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Bam</em>HI </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">22 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126001</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">hexa-His</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
 
 +
                <p>BBa_K3126006 (pSB1C3-pGPM1/tTDH1/ nixA):</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126002</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Bam</em>HI </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">22 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126002</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">nixA</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <!--  BBa_K3126007 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2):-->
 +
                <p>BBa_K3126007 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2):</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126003</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Bam</em>HI </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">22 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa__K3126003</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">TgMTP1t2</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <!--  BBa_K3126008 (pSB1C3-pTEF2/tENO2/ kanR):-->
 +
                <p>BBa_K3126008 (pSB1C3-pTEF2/tENO2/ kanR):</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126003</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Bam</em>HI </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">22 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126004</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">kanR</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <div>
 +
                        <img src="https://2019.igem.org/wiki/images/f/f0/T--HBUT-China--photoPlasmid--figure--BBa-k3126005.png" width="49%">
 +
                        <img src="https://2019.igem.org/wiki/images/1/15/T--HBUT-China--photoPlasmid--figure--BBa-k3126006.png" width="49%">
 +
                    </div>
 +
                    <div>
 +
                        <img src="https://2019.igem.org/wiki/images/6/65/T--HBUT-China--photoPlasmid--figure--BBa-k3126007.png" width="49%">
 +
                        <img src="https://2019.igem.org/wiki/images/c/cb/T--HBUT-China--photoPlasmid--figure--BBa-k3126008.png" width="49%">   
 +
                    </div>
 +
            </div>
 +
            <div id="444">
 +
                <div id="date4" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.6.20 ~ 6.30</h1>
 +
                <h4>Construction of plasmids for the translation and expression of genes in <em>S. cerevisiae:</em></h4>
 +
                <el-collapse v-model="activeNames" @change="handleChange">
 +
                    <el-collapse-item name="41">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126009 (pSB1C3-pTEF1/ tCYC1/ hexa-His/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126009</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word;">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="42">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126010 (pSB1C3-pGPM1/tTDH1/ nixA/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126010</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="43">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126011 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126011</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                </el-collapse>
 +
                <p style="word-wrap:break-word">
 +
                    The resistance gene(kanR) was linked to the target gene(hexa-His, nixA, TgMTP1t2) to screen the engineering yeast and the original yeast.
 +
                </p>
 +
                <p>BBa_K3126009 (pSB1C3-pTEF1/ tCYC1/ hexa-His/ kanR):</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126005</td><td width="300">25 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Spe</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126008</td><td width="300">25 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Xba</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
                <br />
 +
                <p>The enzyme system we use:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126005 linear plasmid</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">kanR part</td><td width="300">6 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub> DNA Ligase</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub>Buffer</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
 
 +
                <p>BBa_K3126010 (pSB1C3-pGPM1/tTDH1/ nixA/ kanR)</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126006</td><td width="300">25 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Spe</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126008</td><td width="300">25 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"> XbaI </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
 
 +
                <br />
 +
                <p>The enzyme system we use:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126006 linear plasmid</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">kanR part</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub> DNA Ligase</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub>Buffer</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <!--  BBa_K3126007 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2):-->
 +
                <p>BBa_K3126011 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2/ kanR)</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126007</td><td width="300">25 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Spe</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126008</td><td width="300">25 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">XbaI </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Pst</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"> Cutsmart Buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">14 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
                <br />
 +
                <p>The enzyme system we use:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126007 linear plasmid</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">kanR part</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub> DNA Ligase</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">T<sub>4</sub>Buffer</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">0.5 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
               
 +
                <div>
 +
                    <img src="https://2019.igem.org/wiki/images/3/31/T--HBUT-China--photoPlasmid--figure--BBa-k3126009.png" width="49%">
 +
                    <img src="https://2019.igem.org/wiki/images/b/ba/T--HBUT-China--photoPlasmid--figure--BBa-k3126010.png" width="49%">
 +
                    <img src="https://2019.igem.org/wiki/images/6/6e/T--HBUT-China--photoPlasmid--figure--BBa-k3126011.png" width="49%">
 +
                </div>
 +
            </div>
 +
            <div id="555">
 +
                <div id="date5" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.7.1 ~ 7.10</h1>
 +
                <h4>Construction of plasmids for the translation and expression of genes in <em>S. cerevisiae:</em></h4>
 +
                <el-collapse v-model="activeNames" @change="handleChange">
 +
                    <el-collapse-item name="51">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126012 (pSB1C3- hexa-His/ nixA/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126012</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word;">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGG<strong><span style="color:#00B050">GCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACG</span></strong>AGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="52">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126013 (pSB1C3- nixA/ TgMTP1t2/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126013</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                          GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                    <el-collapse-item name="53">
 +
                        <template slot="title">
 +
                            <h4>BBa_K3126014 (pSB1C3- hexa-His/ nixA/ TgMTP1t2/ kanR)&nbsp;&nbsp;&nbsp;</h4>
 +
                            <p style="font-size:16px">The sequence of BBa_K3126014</p>
 +
                        </template>
 +
                        <p style="word-wrap:break-word">
 +
                            GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
 +
                        </p>
 +
                    </el-collapse-item>
 +
                </el-collapse>
 +
                <p style="word-wrap:break-word">
 +
                    We obtained the fragments to be inserted by PCR, then combine several target genes by seamless cloning.
 +
                </p>
 +
                <p>BBa_K3126012 (pSB1C3- hexa-His/ nixA/ kanR):</p>
 +
                <p>The PCR system of pGPM1-nixA-tTDH1:</p>
 +
                <p>(P-nixA-T_F</p>
 +
                <p><strong>CGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACA</strong></p>
 +
                <p>P-nixA-T_R</p>
 +
                <p>ACCTATCAACTCTAGTA<strong>AGGCCT</strong>CGTTCAGGGTAATATATTTT)</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126006/SpeⅠ <em>Pst</em>Ⅰ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">P-nixA-T_F </td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">P-nixA-T_R</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">2×PCR mix</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">15 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126009</td><td width="300">15 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Stu</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer </td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">27 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126009/StuⅠ</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">pGPM1-nixA-tTDH1</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
 
 +
                <p>BBa_K3126013 (pSB1C3- nixA/ TgMTP1t2/ kanR)</p>
 +
                <p>The PCR system of pTDH3-TgMTP1t2-tPGK1</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126007/SpeⅠ <em>Pst</em>Ⅰ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">P-TgMTP1t2-T_F</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">P-TgMTP1t2-T_R</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">2×PCR mix</td><td width="300">20 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">15 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126010</td><td width="300">15 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"> <em>Stu</em>>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer </td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">27 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
 
 +
                <br />
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126010/StuⅠ</td><td width="300"> 2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">pTDH3-TgMTP1t2-tPGK1</td><td width="300">4 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
 
 +
                <p>BBa_K3126014 (pSB1C3- hexa-His/ nixA/ TgMTP1t2/ kanR)</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126012</td><td width="300">15 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Stu</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">27 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <p>Seamless cloning system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K3126012/StuⅠ</td><td width="300">2 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">pTDH3-TgMTP1t2-tPGK1 </td><td width="300">4 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">5×CEⅡ Buffer</td><td width="300">2 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">Exnase Ⅱ</td><td width="300">1 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">1 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
 
 +
                <br />
 +
               
 +
                <div>
 +
                    <img src="https://2019.igem.org/wiki/images/4/4e/T--HBUT-China--photoPlasmid--figure--BBa-k3126014.png" width="49%">
 +
                    <img src="https://2019.igem.org/wiki/images/3/3b/T--HBUT-China--photoPlasmid--figure--BBa-k3126013.png" width="49%">
 +
                    <img src="https://2019.igem.org/wiki/images/6/65/T--HBUT-China--photoPlasmid--figure--BBa-k3126012.png" width="49%">
 +
                </div>
 +
            </div>
 +
            <div id="666">
 +
                <div id="date6" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.7.20 ~ 7.25</h1>
 +
                <h4>The plasmid is digested with Bsa1 to get the fragment to be integrated.</h4>
 +
                <p>pSB1C3- hexa-His- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                    <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                        <tbody>
 +
                            <tr>
 +
                                <td width="300">BBa_K3126009</td><td width="300">25μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">buffer</td><td width="300">5 μL</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
                <br/>
 +
                <div style="text-align: center">
 +
                    <img src="https://2019.igem.org/wiki/images/4/48/T--HBUT-China--photoBBa-k3126004.png" width="400">
 +
                </div>
 +
                <p>pSB1C3-nixA- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K31260010</td><td width="300">25μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                <br />
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/a/a8/T--HBUT-China--photoBBa-k3126010.png" width="400">
 +
                </div>
 +
 
 +
                <p>pSB1C3-TgMTP1t2- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K31260011</td><td width="300">25μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                <br />
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/0/0d/T--HBUT-China--photoBBa-k3126011.png" width="400">
 +
                </div>
 +
 
 +
                <p>pSB1C3-hexa-His-nixA- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K31260012</td><td width="300">25μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                <br />
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/9/9e/T--HBUT-China--photoBBa-k3126012.png" width="400">
 +
                </div>
 +
 
 +
                <p>pSB1C3-nixA-TgMTP1t2- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K31260013</td><td width="300">25μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                <br />
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/d/dd/T--HBUT-China--photoBBa-k3126013.png" width="400">
 +
                </div>
 +
 
 +
                <p>pSB1C3-hexa-His-nixA-TgMTP1t2- kanR/ BsaⅠ</p>
 +
                <p>Enzyme digestion system:</p>
 +
                <div style="width:600px;margin:0 auto">
 +
                        <table width="600" border="1" cellspacing="0" cellpadding="0" style="text-align:center">
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td width="300">BBa_K31260014</td><td width="300">25μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300"><em>Bsa</em>I </td><td width="300">3 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">buffer</td><td width="300">5 μL</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td width="300">ddH<sub>2</sub>O</td><td width="300">17 μL</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                <br />
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/e/ec/T--HBUT-China--photoBBa-k3126014.png" width="400">
 +
                </div>
 +
            </div>
 +
            <div id="777">
 +
                <div id="date1" style="position: relative;top:-100px"></div>
 +
                <h1 class="greenp">2019.7.25 ~ 8.5</h1>
 +
                <h4>The fragments were transformed into <em>S. cerevisiae</em> to express the corresponding protein.</h4>
 +
               
 +
                <div style="text-align: center">
 +
                <img src="https://2019.igem.org/wiki/images/d/d3/T--HBUT-China--photoIntegration.png" width="500">
 +
                </div>
 +
 
 +
            </div>
 +
            <div style="position: fixed;top:30vh;right: 3vw;background-color: #adadad65;border-radius: 5px;width: 15%">
 +
                <h3 style="margin: 10px;text-align: center">option date</h3>
 +
                <ul style="list-style: none;padding:0 30px 20px;margin: 0">
 +
                        <li style="margin: 5px ;">
 +
                                <el-link @click="scrollEle('date1')"><strong style="font-size: 18px">Notebook</strong></el-link>
 +
                        </li>
 +
                        <li>
 +
                        <ul style="list-style: none;padding:0 10px 20px;margin: 0">
 +
                                <li v-for="item in navList" style="margin: 5px ;">
 +
                                    <el-link  @click="scrollEle(item.distance)"><strong style="font-size: 16px">{{item.data}}</strong></el-link>
 +
                                </li>
 +
                            </ul>
 +
                        </li>
 +
                </ul>
 +
            </div>
 +
        </div>
 +
    </div>
 +
  <script src="https://2019.igem.org/wiki/index.php?title=Template:HBUT-China/vue.js&action=raw&ctype=text/javascript"></script>
 +
    <script src="https://2019.igem.org/wiki/index.php?title=Template:HBUT-China/element.js&action=raw&ctype=text/javascript"></script>
 +
<script src="https://2019.igem.org/wiki/index.php?title=Template:HBUT-China/NoteBook.js&action=raw&ctype=text/javascript"></script>
 +
</html>

Latest revision as of 02:24, 16 October 2019

2019.5

We synthesized:

promoter TEF1 and terminator CYC1,  hexa-His gene

promoter GPM1 and terminator TDH1,  nixA gene

promoter TDH3 and terminator PGK1,  TgMTP1t2 gene

promoter TEF2 and terminator ENO2,   kanR gene

Each set of promoter terminators corresponds to a target gene.

GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCG

GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCAG

GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCAG

GAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCAG

GGTCTCAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGAGACC

GGTCTCACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGAGAGACC

GGTCTCAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGAGAGACC

GGTCTCACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTAGAGACC

2019.6.1 ~ 6.10

Construction plasmids：

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA

We insert promoter TEF1 and terminator CYC1, promoter GPM1 and terminator TDH1, promoter TDH3 and terminator PGK1, promoter TEF2 and terminator ENO2 into vector pSB1C3 using restriction endonuclease and T4 DNA ligase, respectively.

The pSB1C3 digestion system we used:

pSB1C3 15 μL
EcoRI 3 μL
PstI 3 μL
Cutsmart Buffer 5 μL
ddH2O 24 μL


The enzyme system we use:

pSB1C3 2 μL
fragment 6 μL
T4 DNA Ligase 0.5 μL
T4 Buffer 1 μL
ddH2O 0.5 μL

BBa_k3126001:

BBa_k3126002:

BBa_k3126003:

BBa_k3126004:

2019.6.10 ~ 6.20

Construction plasmids：

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

Insertion of gene into plasmid carrying promoter and terminator.

BBa_K3126005 (pSB1C3-pTEF1/ tCYC1/ hexa His):

Enzyme digestion system:

BBa_K312600120 μL
BamHI 3 μL
buffer5 μL
ddH2O22 μL

Seamless cloning system:

BBa_K3126001 2 μL
hexa-His4 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

BBa_K3126006 (pSB1C3-pGPM1/tTDH1/ nixA):

Enzyme digestion system:

BBa_K312600220 μL
BamHI 3 μL
buffer5 μL
ddH2O22 μL

Seamless cloning system:

BBa_K3126002 2 μL
nixA4 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

BBa_K3126007 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2):

Enzyme digestion system:

BBa_K312600320 μL
BamHI 3 μL
buffer5 μL
ddH2O22 μL

Seamless cloning system:

BBa__K3126003 2 μL
TgMTP1t24 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

BBa_K3126008 (pSB1C3-pTEF2/tENO2/ kanR):

Enzyme digestion system:

BBa_K312600320 μL
BamHI 3 μL
buffer5 μL
ddH2O22 μL

Seamless cloning system:

BBa_K3126004 2 μL
kanR4 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

2019.6.20 ~ 6.30

Construction of plasmids for the translation and expression of genes in S. cerevisiae:

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

The resistance gene(kanR) was linked to the target gene(hexa-His, nixA, TgMTP1t2) to screen the engineering yeast and the original yeast.

BBa_K3126009 (pSB1C3-pTEF1/ tCYC1/ hexa-His/ kanR):

Enzyme digestion system:

BBa_K312600525 μL
SpeI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

BBa_K312600825 μL
XbaI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

The enzyme system we use:

BBa_K3126005 linear plasmid 2 μL
kanR part6 μL
T4 DNA Ligase0.5 μL
T4Buffer1 μL
ddH2O0.5 μL

BBa_K3126010 (pSB1C3-pGPM1/tTDH1/ nixA/ kanR)

Enzyme digestion system:

BBa_K312600625 μL
SpeI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

BBa_K312600825 μL
XbaI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

The enzyme system we use:

BBa_K3126006 linear plasmid 2 μL
kanR part4 μL
T4 DNA Ligase0.5 μL
T4Buffer1 μL
ddH2O0.5 μL

BBa_K3126011 (pSB1C3-pTDH3/tPGK1/ TgMTP1t2/ kanR)

Enzyme digestion system:

BBa_K312600725 μL
SpeI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

BBa_K312600825 μL
XbaI 3 μL
PstI 3 μL
Cutsmart Buffer5 μL
ddH2O14 μL

The enzyme system we use:

BBa_K3126007 linear plasmid 2 μL
kanR part4 μL
T4 DNA Ligase0.5 μL
T4Buffer1 μL
ddH2O0.5 μL

2019.7.1 ~ 7.10

Construction of plasmids for the translation and expression of genes in S. cerevisiae:

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA

We obtained the fragments to be inserted by PCR, then combine several target genes by seamless cloning.

BBa_K3126012 (pSB1C3- hexa-His/ nixA/ kanR):

The PCR system of pGPM1-nixA-tTDH1:

(P-nixA-T_F

CGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACA

P-nixA-T_R

ACCTATCAACTCTAGTAAGGCCTCGTTCAGGGTAATATATTTT)

BBa_K3126006/SpeⅠ PstⅠ1 μL
P-nixA-T_F 2 μL
P-nixA-T_R2 μL
2×PCR mix20 μL
ddH2O15 μL

Enzyme digestion system:

BBa_K312600915 μL
StuI 3 μL
buffer 5 μL
ddH2O27 μL

Seamless cloning system:

BBa_K3126009/StuⅠ 2 μL
pGPM1-nixA-tTDH14 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

BBa_K3126013 (pSB1C3- nixA/ TgMTP1t2/ kanR)

The PCR system of pTDH3-TgMTP1t2-tPGK1

BBa_K3126007/SpeⅠ PstⅠ1 μL
P-TgMTP1t2-T_F2 μL
P-TgMTP1t2-T_R2 μL
2×PCR mix20 μL
ddH2O15 μL

Enzyme digestion system:

BBa_K312601015 μL
Stu>I 3 μL
buffer 5 μL
ddH2O27 μL

Seamless cloning system:

BBa_K3126010/StuⅠ 2 μL
pTDH3-TgMTP1t2-tPGK14 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

BBa_K3126014 (pSB1C3- hexa-His/ nixA/ TgMTP1t2/ kanR)

Enzyme digestion system:

BBa_K312601215 μL
StuI 3 μL
buffer5 μL
ddH2O27 μL

Seamless cloning system:

BBa_K3126012/StuⅠ2 μL
pTDH3-TgMTP1t2-tPGK1 4 μL
5×CEⅡ Buffer2 μL
Exnase Ⅱ1 μL
ddH2O1 μL

2019.7.20 ~ 7.25

The plasmid is digested with Bsa1 to get the fragment to be integrated.

pSB1C3- hexa-His- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K312600925μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

pSB1C3-nixA- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K3126001025μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

pSB1C3-TgMTP1t2- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K3126001125μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

pSB1C3-hexa-His-nixA- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K3126001225μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

pSB1C3-nixA-TgMTP1t2- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K3126001325μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

pSB1C3-hexa-His-nixA-TgMTP1t2- kanR/ BsaⅠ

Enzyme digestion system:

BBa_K3126001425μL
BsaI 3 μL
buffer5 μL
ddH2O17 μL

2019.7.25 ~ 8.5

The fragments were transformed into S. cerevisiae to express the corresponding protein.

option date

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