Latest revision as of 21:09, 19 October 2019

<!DOCTYPE html> Cheaters · Benchling

Cheaters

Project: Survivors

Authors: iGEM_GO_Paris_saclay

Monday, 29/7/2019

Some bacteria can escape DNA degradation. We called these survivor cells cheaters. Following assays aim to characterize the proportion of bacteria that escapes DNA cleavage and mecanisms leading to this escape.

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Principle: to characterize if this new phenotype was due to a mutation carried by the plasmid, we extract the plasmid of the cheater cells and sequence it. In the same time, we also performe CFU plus we take the OD of these cheaters.

Production of M9 medium (Colombe - Laura)

15mL of M9 salt (10x)

3 mL of Glucose 20%

1,5mL of CAA 20%

150μL of MgSo4 (1M)

1,5μL of CaCl2 (1M)

150μL of Biotin (1mg/mL)

150μL of Thiamin (1mg/mL)

1,5mL of Trace element (100x)

128,5mL of (200mL of H20 + 3,5gr of Agar)

Toxicity of B. sub / DH5α (Marie - Laura)

Indution of survivors A1 et T7

Preparation of pre-culture :

3mL of LB

30μL of Glucose

3μL of Amp

+ stamp

Different stamp :

1.

Bacilus subtilis from glycerol stock

2.

Moclo from glycerol stock

3.

A1 from glycerol stock

4.

T7 from glycerol stock

5.

T7 from the box "16/07 iGEM LB+Glu+Amp T7 - Ara Lendemain"

6.

A1 from the box "16/07 iGEM LB+Glu+Amp A1 - Ara Lendemain"

put overnight at 37°C with agitation.

Tuesday, 30/7/2019

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Toxicity of B. sub / DH5α + Induction : Marie_Laura

From a culture that has grown overnight, dilute to an OD of 0.1 in 15mL LB + Amp, then grow at 37°C with agitation to an OD of 0.4. Take OD, it's the time t0.

1.

Bacilus subtilis from DH5alpha glycerol stock = B.sub

2.

KeioZ1F' + PBAD24-Moclo from glycerol stock = Moclo

3.

KeioZ1F' + PBAD24-A1 from glycerol stock = A1

4.

KeioZ1F' + PBAD24-T7 from glycerol stock = T7

5.

KeioZ1F' + PBAD24-T7 from the box "16/07 iGEM LB+Glu+Amp T7 - Ara Lendemain" = "tricheur"T7 = TT7

6.

KeioZ1F' + PBAD24-A1 from the box "16/07 iGEM LB+Glu+Amp A1 - Ara Lendemain" = "tricheur"A1 = TA1

Add 0,2% of Arabinose. Then let it grow at 37°C and take the OD after 30min, 1h30, 2h30 and the next day. At the same time make droplets of different dilutions: from 10-1 to 10^-7.

Results :

	A A	B B	C C	D D	E E	F F	G G
1 1		T7	TT7	A1	TA1	Moclo	B.sub
2 2	t0min	0,53	0,43	0,54	0,38	0,72	0,53
3 3	t30min	0,49	0,62	0,71	0,65	0,93	0,68
4 4	t1h30	0,43	1,06	0,67	1,12	1,46	0,88
5 5	t2h30	0,41	1,47	0,68	1,69	1,87	0,85
6 6	overnight	2,7	3,5	3,1	4,1	3,8	5,9

Table2

Wednesday, 31/7/2019

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Toxicity of B. sub / DH5α + Induction: Marie_Laura

Isolation of T7 and TA1 (=cheater T7 and A1) in a new agar plate with LB+Amp+Glucose to attempt an isolate of a single colonies.

Agar plates are then incubated at 37°C overnight.

Results of the spreading boxs from transformers' solutions.

	A A	B B	C C	D D	E E	F F
1 1	Time	Omin	30min	90min	150min	Tomorow
2 2	Cheater A1	10^-5	10^-5	10^-6	10^-6	10^-6
3 3		5	5	3	16	12
4 4		8	5	2	14	14
5 5		11	11	5	13	15
6 6		8	8	10	16	14
7 7	Cheater T7	10^-5	10^-5	10^-6	10^-6	10^-6
8 8		8	11	4	9	20
9 9		6	13	6	11	19
10 10		11	13	6	16	21
11 11		9		8	9	19

CFU in 10^8 (cell/mL)

Monday, 5/8/2019

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Preculture for nuclease characterization (CFU and O.D.) : Arnaud

4 precultures are put at 37°C o/v : keioZ1F' pBAD24_A1 ; keioZ1F' pBAD24_Moclo ; and 2 keioZ1F' pBAD24_A1 survivors from a previous nuclease induction.

Each preculture is composed of 3 mL LB + ampiciline + glucose

Tuesday, 6/8/2019

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Nucleases characterization and cheater study : Arnaud

Culture of A1, Moclo and A1 cheater (they survided the nuclease induction from a previous induction but not a single clone but many were used for the preculture) in LB + Amp (25mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4.

O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)

Results :

	A A	B B	C C	D D	E E	F F	G G	H H
1 1	O.D
2 2	Time Point	10h14	10h44 (-30')	11h14 (T0) Arabinose is added	11h44 (+30')	12h44 (+90')	13h44 (+150')	TOMORROW 12h15 (+1351)
3 3	A1	0.1	0.16	0.32	0.59	0.57	0.58	3.00
4 4	Moclo	0.1	0.18	0.40	0.66	1.18	1.64	2.40
5 5	A1 Cheater	0.1	0.20	0.42	0.69	1.20	1.66	/

O.D Measurement

	A A	B B	C C	D D	E E	F F
1 1	CFU	Time (min)
2 2	Bacteria	-30	30	90	150	1351
3 3	A1	-6	-2
4 4		1	5
5 5			4
6 6	Moclo			-6	-6	-7
7 7				12	10	33
8 8				15	7	22
9 9	Survivor A1 N°3	-5		-7	-6
10 10		12		1	12
11 11				7	6

Colony number at different time for each condition and dilution factor associated

Most of the results are not usable because the droplet mixed themself together

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Precultures of one clone pBAD24 Moclo, pBAD24 T7 and 2 survivors after induction of the nuclease A1 : Clara

Each strains were put into 15mL of LB + amp (15µL) + glucose (150µL)

The survivors/cheaters were taking from the plate 07/16 for the "day after" condition, #2 clone 5 and #1 clone4

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Culture of DH5alpha to make ultra competent cells : Laetitia

1mL from the day before preculture (05/08/2019) has been added to 250mL of LB and let grown at 20°C, 140 rpm until D0₆₀₀=0.55

Wednesday, 7/8/2019

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Nucleases T7 characterization and A1 cheaters study : Arnaud_Clara

Culture of Moclo T7 (*2) and A1 cheaters clone 4 and 5 (they survided the nuclease induction from a previous induction but not a single clone but many were used for the preculture) in LB + Amp (25mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4. O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)

Results :

	A A	B B	C C	D D	E E	F F	G G	H H
1 1	O.D.
2 2	Time point	11h20	11h50 (-30')	12h20 (T0)	12h50 (+30')	13h50 (+90')	14h50 (+150')	TOMORROW
3 3	Moclo	0.1	0.29	0.53	0.87	1.53	1.94	4.5
4 4	T7 n°1	0.1	0.20	0.32	0.42	0.36	0.29	3.8
5 5	T7 n°2	0.1	0.25	0.41	0.42	0.38	0.36	3.9
6 6	Cheater A1 clone 4	0.1	0.31	0.52	0.85	1.51	1.87	3.7
7 7	Cheater A1 clone 5	0.1	0.29	0.47	0.59	0.58	0.59	3.4

Table1

	A A	B B	C C	D D	E E	F F
1 1	CFU	Time (min)
2 2	Bacteria	-30	30	90	150	TOMORROW
3 3	Moclo		-5	-5	-6	-5
4 4			27	26	19	27
5 5			26	33	12	26
6 6			27	29	16	27
7 7	T7 N°1	-4	-1	-1	-1	-5
8 8		19	14	10	21	7
9 9		10	7	6	29	4
10 10		12	15	5	22	5
11 11	T7 N°2	-4	-1	-1	-1	-3
12 12		18	15	17	25	16
13 13		12	18	25		18
14 14		19	20	19		11
15 15	Survivor A1 N° 4	-4	-5	-5	-6	-5
16 16		22	21	17	15	20
17 17		32	28	17	12	34
18 18		14	22	12	9	28
19 19	Survivor A1 N° 5	-4	-2	-1	-1	-2
20 20		26	22	17		2
21 21		15	26	15		1
22 22		18	7			4

CFU

Friday, 9/8/2019

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Plasmid extraction : Arnaud

Extraction of plasmids from :

a.

Cheater (survivor of previous nuclease induction) A1 clone 1

b.

Cheater A1 clone 4

c.

Cheater T7 clone 1

Use of Macherey-Nagel kit "DNA and RNA purification" protocol page 18 "Low copy"

Analysis with Nanodrop :

	A A	B B
1 1	Plasmid from	Concentration (ng/µL)
2 2	Cheater A1 clone 1	149
3 3	Cheater A1 clone 4	169
4 4	Cheater T7 clone 1	184

Nanodrop Results 9/08

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Heatshock plasmid Transformation : Arnaud

5 transformations are done : 2 are double transformations and the other are simple transformation. 1 µL per plasmid and 50 µL of competent KeioZ1F' :

I.

KeioZ1F' with plasmid from cheater A1 clone 4 (previously extracted)

II.

KeioZ1F' with plasmid from cheater T7 clone 1 (previously extracted)

●

Preculture : Arnaud

Preculture of KeioZ1F'_A1, Moclo, T7, B.sub, KeioZ1F' transformed with plasmid from Cheater A1 clone 4 and KeioZ1F' transformed with plasmid from Cheater T7 clone 1 3 mL LB + Ampiciline + Glucose 0.2% overnight with shaking at 37°C.

Monday, 12/8/2019

●

Nucleases caracterization and cheater study : Arnaud

Culture of A1, Moclo, T7 , B.sub, KeioZ1F' transformed with plasmid from Cheater A1 clone 4 and KeioZ1F' transformed with plasmid from Cheater T7 clone 1 in LB + Amp (50mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4.

O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)

Arabinose added at 12h10 (Cf = 0.2%)

Results :

	A A	B B	C C	D D	E E	F F	G G	H H
1 1	Time point	10h55	11h30 (t-30')	12h00 (t0)	12h40 (t+30')	13h40 (t+90')	14h40 (t+150')	Tomorrow 11h15 (t+1395')
2 2	A1	0.1	0.18	0.38	0.55	0.51	0.55	3.6
3 3	T7	0.1	0.15	0.17	0.20	0.20	0.17	3.6
4 4	Moclo	0.1	0.19	0.34	0.71	1.31	1.83	3.6
5 5	B.sub	0.1	0.13	0.21	0.38	0.61	0.58	4.6
6 6	KeioZ1F' transformed with pasmid from Cheater A1 clone 4	0.1	0.21	0.40	0.68	1.38	1.80	3.6
7 7	KeioZ1F' transformed with pasmid from Cheater T7 clone 1	0.1	0.16	0.35	0.63	1.23	1.75	3.3

O.D. 12/08

	A A	B B	C C	D D	E E	F F
1 1	CFU	Time (min)
2 2	Bacteria	-30	30	90	150	1395
3 3	Moclo	-5	-5	-5	-6
4 4		12	8	27	8
5 5		2	9		13
6 6					5
7 7	B.sub	-4	-4	-3	-2
8 8		20	25	11	31
9 9		16	30	10	30
10 10		21	28	15	24
11 11	T7	-3	-2	-1	-2
12 12		4	19	No colony after	2
13 13		8	20		4
14 14		5	19
15 15	A1	-4	-1	-1	-1
16 16		14	No colony after	No colony after	No colony after
17 17		16
18 18
19 19	KeioZ1F' transformed with pasmid from Cheater A1 clone 4	-4	-5	-5	-6
20 20		10	12	28	7
21 21		15	14	29	15
22 22			13		11
23 23	KeioZ1F' transformed with pasmid from Cheater T7 clone 1	-4	-5	-6	-6
24 24		15	8	25	8
25 25		16	14	29	9
26 26		20	13	16	8

CFU 12/08

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-    </style><div><div><div class="printable-container hide-print-links" data-printable-left-margin="38"><div class="printable-container-boundingBox"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1"><h1 class="mediocre-titleEditor"><span class="mediocre-titleEditor-header"><span class="mediocre-titleEditor-header-id u-faded"> </span></span><div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Phage infection assay</div></h1><div><div><div><b>Project:</b> Phage multiplication</div><div><b>Authors:</b> iGEM_GO_Paris_saclay</div></div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator" data-scroll-id="entryDay842"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 16/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part872"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part872" data-index="0" style="margin-left: 0px;">AIM: As a first step in proving the RNA World concept, we wanted to create an “RNA cell”, in which replicating RNA, rather than DNA, would be the support of genetic information.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part873"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part873" data-index="1" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part874"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part874" data-index="2" style="margin-left: 0px;"><u><span style="color:#000000">Principle</span></u><span style="color:#000000">: In order to carry out this substitution of DNA, we clone and expresse 3 nucleases: A1, gp3 and yqcg and see if they can efficiently degrade bacterial DNA. In addition to these nucleases, we infecte the cells by <span style="background-color:#FFFFFF">the MS2 phage and produce new virions, which would result from the replication of RNA and its translation into proteins, since MS2 is an </span><span style="background-color:#FFFFFF">positive-sense single-stranded </span></span><a href="https://en.wikipedia.org/wiki/RNA" rel="nofollow noopener noreferrer" target="_blank"><span style="color:#000000"><span style="background-color:#FFFFFF">RNA</span></span></a><span style="color:#000000"><span style="background-color:#FFFFFF"> phage</span></span><span style="background-color:#FFFFFF"><span style="color:#000000">.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part875"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part875" data-index="3" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part876"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part876" data-index="4" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part877"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part877" data-index="5" style="margin-left: 35px;"><b>MS2 phage titration : Hugues</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part878"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part878" data-index="6" style="margin-left: 0px;">Incubate K12 Hfr in 5mL LB-amp (0,2%) initial OD600=0,1 at 37°C</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part879"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part879" data-index="7" style="margin-left: 0px;">Cultivate with agitation to OD600=0,8</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part880"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part880" data-index="8" style="margin-left: 0px;">Melt agar, add CaCl2 and MgCl2 (1mM final), keep the mix at 42°C</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part881"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part881" data-index="9" style="margin-left: 0px;">Pour 3mL agar-CaCl2-MgCl2 in sterile glass tubes</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part882"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part882" data-index="10" style="margin-left: 0px;">Add 100µL bacteria (K12 Hfr) + 100µL MS2 phage </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part883"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part883" data-index="11" style="margin-left: 0px;">Pour the 3mL agar(CaCl2-MgCl2-bacteria-phage on the LB plate, spread the mix</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part884"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part884" data-index="12" style="margin-left: 0px;">Incubate at 37°C</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part885"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part885" data-index="13" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part886"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part886" data-index="14" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay844"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 18/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part887"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part887" data-index="15" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part888"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part888" data-index="16" style="margin-left: 0px;"><b>MS2 infection : Hugues</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part889"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part889" data-index="17" style="margin-left: 0px;">KeioZ1, KeioZ1 F' MoClo, KeioZ1 F' A1, KeioZ1 F' T7</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part890"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part890" data-index="18" style="margin-left: 0px;">Dilute these 4 strains in LB (amp if necessary) in 12 mL LB at 0D600=0,1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part891"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part891" data-index="19" style="margin-left: 0px;">Add CaCl2 and MgCl2 (1 mM final)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part892"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part892" data-index="20" style="margin-left: 0px;">Incubate at 37°C with agitation (200rpm) to OD=0,4</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part893"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part893" data-index="21" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part894"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part894" data-index="22" style="margin-left: 0px;">40min after the incubation of the 4 strains, dilute K12 Hfr strain in 12mL LB at OD=0,1 (no CaCl2, MgCl2)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part895"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part895" data-index="23" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part896"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part896" data-index="24" style="margin-left: 0px;">When Keio strains are at OD=0,4, add arabinose (0,2% final) and incubate at 37°C for 30min</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part897"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part897" data-index="25" style="margin-left: 0px;">Mesurate OD600 then add phage volume equals to : V(µL) = [5*vol culture bacteria (in mL)*OD600*5*10^8]/phage concentration</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part898"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part898" data-index="26" style="margin-left: 0px;">-&gt; OD600 30min after arabinose </div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1631" data-scroll-id="note-part899"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part899-item" data-formattable="true" data-index="27" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="27" data-printable-layout-width="353" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part899-table" data-formattable="true" data-index="27" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 345px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 333px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 88px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-0-0" data-namespace="table-ns1567"><span>KeioZ1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-0-1" data-namespace="table-ns1567"><span>0,65</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-0-2" data-namespace="table-ns1567"><span>466,5µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-1-0" data-namespace="table-ns1567"><span>KeioZ1 F' MoClo </span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-1-1" data-namespace="table-ns1567"><span>0,78</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-1-2" data-namespace="table-ns1567"><span>559,8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-2-0" data-namespace="table-ns1567"><span>KeioZ1 F' A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-2-1" data-namespace="table-ns1567"><span>0,49</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-2-2" data-namespace="table-ns1567"><span>551,7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-3-0" data-namespace="table-ns1567"><span>KeioZ1 F' T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-3-1" data-namespace="table-ns1567"><span>0,56</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part899-3-2" data-namespace="table-ns1567"><span>401,9</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table7</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part899" data-index="27" style="max-width: 353px; margin-left: 0px;">
+    </style><div><div><div class="printable-container hide-print-links" data-printable-left-margin="38"><div class="printable-container-boundingBox"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1"><h1 class="mediocre-titleEditor"><span class="mediocre-titleEditor-header"><span class="mediocre-titleEditor-header-id u-faded"> </span></span><div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Cheaters</div></h1><div><div><div><b>Project:</b> Survivors</div><div><b>Authors:</b> iGEM_GO_Paris_saclay</div></div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator" data-scroll-id="entryDay842"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 29/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part868"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part868" data-index="0" style="margin-left: 0px;">Some bacteria can escape DNA degradation. We called these survivor cells cheaters. Following assays aim to characterize the proportion of bacteria that escapes DNA cleavage and mecanisms leading to this escape.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part869"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part869" data-index="1" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part870"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part870" data-index="2" style="margin-left: 35px;">Principle: to characterize if this new phenotype was due to a mutation carried by the plasmid, we extract the plasmid of the cheater cells and sequence it. In the same time, we also performe CFU plus we take the OD of these cheaters.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part871"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part871" data-index="3" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part872"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part872" data-index="4" style="margin-left: 0px;"><b><u>Production of M9 medium (Colombe - Laura)</u></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part873"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part873" data-index="5" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part874"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part874" data-index="6" style="margin-left: 0px;">15mL of M9 salt (10x)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part875"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part875" data-index="7" style="margin-left: 0px;">3 mL of Glucose 20%</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part876"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part876" data-index="8" style="margin-left: 0px;">1,5mL of CAA 20%</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part877"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part877" data-index="9" style="margin-left: 0px;">150<span style="color:#000000"><span style="background-color:#FFFFFF">μL</span></span> of MgSo4 (1M)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part878"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part878" data-index="10" style="margin-left: 0px;">1,5<span style="color:#000000"><span style="background-color:#FFFFFF">μL of CaCl2 (1M)</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part879"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part879" data-index="11" style="margin-left: 0px;">150<span style="color:#000000"><span style="background-color:#FFFFFF">μL of Biotin (1mg/mL)</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part880"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part880" data-index="12" style="margin-left: 0px;">150<span style="color:#000000"><span style="background-color:#FFFFFF">μL of Thiamin (1mg/mL)</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part881"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part881" data-index="13" style="margin-left: 0px;">1,5mL of Trace element (100x)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part882"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part882" data-index="14" style="margin-left: 0px;">128,5mL of (200mL of H20 + 3,5gr of Agar)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part883"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part883" data-index="15" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part884"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part884" data-index="16" style="margin-left: 0px;"><b><u>Toxicity of B. sub / DH5α (Marie - Laura)</u></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part885"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part885" data-index="17" style="margin-left: 0px;">Indution of survivors A1 et T7</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part886"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part886" data-index="18" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part887"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part887" data-index="19" style="margin-left: 0px;">Preparation of pre-culture :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part888"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part888" data-index="20" style="margin-left: 0px;">3mL of LB</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part889"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part889" data-index="21" style="margin-left: 0px;"><span style="color:#000000"><span style="background-color:#FFFFFF">30μL of Glucose</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part890"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part890" data-index="22" style="margin-left: 0px;"><span style="color:#000000"><span style="background-color:#FFFFFF">3μL of Amp</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part891"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part891" data-index="23" style="margin-left: 0px;">+ stamp</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part892"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part892" data-index="24" style="margin-left: 0px;">Different stamp :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part893"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part893" data-index="25" style="margin-left: 35px;">Bacilus subtilis from glycerol stock</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part894"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part894" data-index="26" style="margin-left: 35px;">Moclo from glycerol stock</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part895"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part895" data-index="27" style="margin-left: 35px;">A1 from glycerol stock</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part896"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>4.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part896" data-index="28" style="margin-left: 35px;">T7 from glycerol stock</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part897"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>5.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part897" data-index="29" style="margin-left: 35px;">T7 from the box  "16/07 iGEM LB+Glu+Amp T7 - Ara Lendemain"</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part898"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>6.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part898" data-index="30" style="margin-left: 35px;">A1 from the box "16/07 iGEM LB+Glu+Amp A1 - Ara Lendemain"</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part899"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part899" data-index="31" style="margin-left: 0px;">put overnight at 37°C with agitation.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part900"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part900" data-index="32" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part901"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part901" data-index="33" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay844"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 30/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part902"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part902" data-index="34" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part903"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part903" data-index="35" style="margin-left: 35px;"><b><span style="color:#344043"><span style="background-color:#FFFFFF">Toxicity of B. sub / DH5α + Induction : Marie_Laura</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part904"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part904" data-index="36" style="margin-left: 0px;"><span style="color:#344043"><span style="background-color:#FFFFFF">From a culture that has grown overnight, dilute to an OD of 0.1 </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">in </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">15mL LB + </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">Amp</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">, then grow at 37°C with agitation to an OD of 0.4. </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">Take OD, it's the time t0.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part905"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part905" data-index="37" style="margin-left: 35px;">Bacilus subtilis from  DH5alpha glycerol stock = B.sub</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part906"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part906" data-index="38" style="margin-left: 35px;">KeioZ1F' + PBAD24-Moclo from glycerol stock = Moclo</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part907"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part907" data-index="39" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">KeioZ1F' + PBAD24-</span></span>A1 from glycerol stock = A1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part908"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>4.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part908" data-index="40" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">KeioZ1F' + PBAD24-</span></span>T7 from glycerol stock = T7</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part909"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>5.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part909" data-index="41" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">KeioZ1F' + PBAD24-</span></span>T7 from the box  "16/07 iGEM LB+Glu+Amp T7 - Ara Lendemain" = "tricheur"T7 = TT7</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part910"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>6.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part910" data-index="42" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">KeioZ1F' + PBAD24-</span></span>A1 from the box "16/07 iGEM LB+Glu+Amp A1 - Ara Lendemain" = "tricheur"A1 = TA1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part911"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part911" data-index="43" style="margin-left: 0px;"><span style="color:#344043"><span style="background-color:#FFFFFF">Add 0,2% of Arabinose</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">. Then </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">let it grow at 37°C and take the OD after 30min, 1h30, 2h30 and the next day. </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">At the same time make droplets of different dilutions: from 10-1 to 10^-7.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part912"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part912" data-index="44" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part913"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part913" data-index="45" style="margin-left: 0px;">Results : </div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1564" data-scroll-id="note-part914"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part914-item" data-formattable="true" data-index="46" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="46" data-printable-layout-width="765" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part914-table" data-formattable="true" data-index="46" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 757px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 745px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-1" data-namespace="table-ns1494"><span>T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-2" data-namespace="table-ns1494"><span>TT7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-3" data-namespace="table-ns1494"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-4" data-namespace="table-ns1494"><span>TA1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-5" data-namespace="table-ns1494"><span>Moclo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-0-6" data-namespace="table-ns1494"><span>B.sub</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-0" data-namespace="table-ns1494"><span>t0min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-1" data-namespace="table-ns1494"><span>0,53</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-2" data-namespace="table-ns1494"><span>0,43</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-3" data-namespace="table-ns1494"><span>0,54</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-4" data-namespace="table-ns1494"><span>0,38</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-5" data-namespace="table-ns1494"><span>0,72</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-1-6" data-namespace="table-ns1494"><span>0,53</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-0" data-namespace="table-ns1494"><span>t30min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-1" data-namespace="table-ns1494"><span>0,49</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-2" data-namespace="table-ns1494"><span>0,62</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-3" data-namespace="table-ns1494"><span>0,71</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-4" data-namespace="table-ns1494"><span>0,65</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-5" data-namespace="table-ns1494"><span>0,93</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-2-6" data-namespace="table-ns1494"><span>0,68</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-0" data-namespace="table-ns1494"><span>t1h30</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-1" data-namespace="table-ns1494"><span>0,43</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-2" data-namespace="table-ns1494"><span>1,06</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-3" data-namespace="table-ns1494"><span>0,67</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-4" data-namespace="table-ns1494"><span>1,12</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-5" data-namespace="table-ns1494"><span>1,46</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-3-6" data-namespace="table-ns1494"><span>0,88</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-0" data-namespace="table-ns1494"><span>t2h30</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-1" data-namespace="table-ns1494"><span>0,41</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-2" data-namespace="table-ns1494"><span>1,47</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-3" data-namespace="table-ns1494"><span>0,68</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-4" data-namespace="table-ns1494"><span>1,69</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-5" data-namespace="table-ns1494"><span>1,87</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-4-6" data-namespace="table-ns1494"><span>0,85</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-0" data-namespace="table-ns1494"><span>overnight</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-1" data-namespace="table-ns1494"><span>2,7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-2" data-namespace="table-ns1494"><span>3,5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-3" data-namespace="table-ns1494"><span>3,1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-4" data-namespace="table-ns1494"><span>4,1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-5" data-namespace="table-ns1494"><span>3,8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part914-5-6" data-namespace="table-ns1494"><span>5,9</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table2</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part914" data-index="46" style="max-width: 765px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part900"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part900" data-index="28" style="margin-left: 0px;">Take off 0,5mL of bacteria at time 0, 1 hour, 2h and 18h. Take OD600 for each culture</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part901"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part901" data-index="29" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1632" data-scroll-id="note-part902"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part902-item" data-formattable="true" data-index="30" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="30" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part902-table" data-formattable="true" data-index="30" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-0-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-0-1" data-namespace="table-ns1567"><span>t0</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-0-2" data-namespace="table-ns1567"><span>t1h</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-0-3" data-namespace="table-ns1567"><span>t2h</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-0-4" data-namespace="table-ns1567"><span>t18h</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-1-0" data-namespace="table-ns1567"><span>KeioZ1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-1-1" data-namespace="table-ns1567"><span>0,65</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-1-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-1-3" data-namespace="table-ns1567"><span>2,6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-1-4" data-namespace="table-ns1567"><span>4,5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-2-0" data-namespace="table-ns1567"><span>KeioZ1 F' MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-2-1" data-namespace="table-ns1567"><span>0,78</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-2-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-2-3" data-namespace="table-ns1567"><span>0,47</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-2-4" data-namespace="table-ns1567"><span>2,7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-3-0" data-namespace="table-ns1567"><span>KeioZ1 F' A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-3-1" data-namespace="table-ns1567"><span>0,49</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-3-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-3-3" data-namespace="table-ns1567"><span>0,51</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-3-4" data-namespace="table-ns1567"><span>1,6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-4-0" data-namespace="table-ns1567"><span>KeioZ1 F' T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-4-1" data-namespace="table-ns1567"><span>0,56</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-4-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-4-3" data-namespace="table-ns1567"><span>0,40</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part902-4-4" data-namespace="table-ns1567"><span>0,6</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table8</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part902" data-index="30" style="max-width: 565px; margin-left: 0px;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part915"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part915" data-index="47" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay846"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 31/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part916"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part916" data-index="48" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part917"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part917" data-index="49" style="margin-left: 35px;"><b><span style="color:#344043"><span style="background-color:#FFFFFF">Toxicity of B. sub / DH5α + Induction: Marie_Laura</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part918"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part918" data-index="50" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part919"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part919" data-index="51" style="margin-left: 0px;"><span style="color:#222222"><span style="background-color:#FFFFFF">Isolation of T7 and TA1 (=cheater T7 and A1) i</span></span><span style="color:#222222"><span style="background-color:#FFFFFF">n a new agar plate with LB+Amp+Glucose to attempt an isolate of a single colonies.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part920"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part920" data-index="52" style="margin-left: 0px;"><span style="color:#222222"><span style="background-color:#FFFFFF">Agar plates are then incubated at 37°C overnight.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part921"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part921" data-index="53" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part922"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part922" data-index="54" style="margin-left: 0px;">Results of the spreading boxs from transformers' solutions.</div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1565" data-scroll-id="note-part923"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part923-item" data-formattable="true" data-index="55" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="55" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part923-table" data-formattable="true" data-index="55" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-0" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>Time</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-1" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>Omin</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-2" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>30min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-3" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>90min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-4" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>150min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-0-5" data-namespace="table-ns1494" style="background-color: rgb(192, 192, 192);"><span>Tomorow</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-0" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>Cheater A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-1" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-2" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-3" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-4" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-1-5" data-namespace="table-ns1494" style="background-color: rgb(99, 170, 254);"><span>10^-6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-2-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-2-1" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-2-2" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-2-3" data-namespace="table-ns1494"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-2-4" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-2-5" data-namespace="table-ns1494"><span>12</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-3-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-3-1" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-3-2" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-3-3" data-namespace="table-ns1494"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-3-4" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-3-5" data-namespace="table-ns1494"><span>14</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-4-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-4-1" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-4-2" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-4-3" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-4-4" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-4-5" data-namespace="table-ns1494"><span>15</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-5-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-5-1" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-5-2" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-5-3" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-5-4" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-5-5" data-namespace="table-ns1494"><span>14</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-0" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>Cheater T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-1" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-2" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-3" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-4" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-6-5" data-namespace="table-ns1494" style="background-color: rgb(255, 245, 140);"><span>10^-6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-7-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-7-1" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-7-2" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-7-3" data-namespace="table-ns1494"><span>4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-7-4" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-7-5" data-namespace="table-ns1494"><span>20</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">9</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">9</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-8-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-8-1" data-namespace="table-ns1494"><span>6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-8-2" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-8-3" data-namespace="table-ns1494"><span>6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-8-4" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-8-5" data-namespace="table-ns1494"><span>19</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">10</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">10</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-9-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-9-1" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-9-2" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-9-3" data-namespace="table-ns1494"><span>6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-9-4" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-9-5" data-namespace="table-ns1494"><span>21</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">11</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">11</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-10-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-10-1" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part923-10-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-10-3" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-10-4" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part923-10-5" data-namespace="table-ns1494"><span>19</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">CFU in 10^8 (cell/mL)</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part923" data-index="55" style="max-width: 665px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part903"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part903" data-index="31" style="margin-left: 0px;">With the 0,5mL, centrifugate at 13000rpm 5 min</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part904"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part904" data-index="32" style="margin-left: 0px;">Take off 180µL of the supernatant, put in a microplaque </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part905"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part905" data-index="33" style="margin-left: 0px;">Dilute 10 fold in series the supernatant (20 in 180) (No dilution to 10^-9)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part906"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part906" data-index="34" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part907"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part907" data-index="35" style="margin-left: 0px;">Prepare 3mL soft agar (3mL for each LB plate needed)(50% solid LB + 50% liquid LB + 1mM CaCl2 + 1mM MgCl2, 100µL K12 Hfr (at OD=0,4)) </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part908"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part908" data-index="36" style="margin-left: 0px;">Pour the 3mL onto LB plate</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part909"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part909" data-index="37" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part910"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part910" data-index="38" style="margin-left: 0px;">Once soft agar dry out, drop off 5µL of each dilution (+ND) on LB + soft agar plate. 37°C</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part911"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part911" data-index="39" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part912"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part912" data-index="40" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay846"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 19/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part913"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part913" data-index="41" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part914"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part914" data-index="42" style="margin-left: 0px;"><b>MS2 phage stock : Hugues</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part915"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part915" data-index="43" style="margin-left: 0px;">Add 10mL phage buffer to a phage titration plate with lysis range. Incubate the Petri dish at room temperature 24h then repipette the phage buffer, filtrate it then stock the MS2 phage stock at 4°C.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part916"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part916" data-index="44" style="margin-left: 0px;">The MS2 phage stock has to be titrated.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part917"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part917" data-index="45" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part918"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part918" data-index="46" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part919"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part919" data-index="47" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay848"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 23/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part920"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part920" data-index="48" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part921"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part921" data-index="49" style="margin-left: 0px;"><b>MS2 phage infection : Hugues</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part922"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part922" data-index="50" style="margin-left: 0px;">Start a culture of KeioZ1, Z1 F' MoClo, Z1 F' A1, Z1 F' T7 in 12mL LB (+amp if necessary) + 1mM CaCl2 + 1mM MgCl2 at OD600=0,1 then incubate at 37°C</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part923"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part923" data-index="51" style="margin-left: 0px;">Start the culture of K12 Hfr in 12 mL LB 30/40min after the first culture </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part924"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part924" data-index="52" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay850"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 31/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part925"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part925" data-index="53" style="margin-left: 0px;">See the protocol "Infection assay" below.</div></div></div><div class="mediocre-item" data-scroll-id="note-part926"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part926-item" data-formattable="true" data-index="54" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Protocol-phage-infection1.docx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part926" data-index="54">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part924"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part924" data-index="56" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay848"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 5/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part925"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part925" data-index="57" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part926"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part926" data-index="58" style="margin-left: 35px;"><b>Preculture for nuclease characterization (CFU and O.D.) : Arnaud</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part927"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part927" data-index="59" style="margin-left: 35px;">4 precultures are put at 37°C o/v : keioZ1F' pBAD24_A1 ; keioZ1F' pBAD24_Moclo ; and 2 keioZ1F' pBAD24_A1 survivors from a previous nuclease induction.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part928"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part928" data-index="60" style="margin-left: 35px;">Each preculture is composed of 3 mL LB + ampiciline + glucose</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part929"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part929" data-index="61" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part930"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part930" data-index="62" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay850"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part931"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part931" data-index="63" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part932"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part932" data-index="64" style="margin-left: 35px;"><b><span style="color:#344043"><span style="color:#222222"><span style="background-color:#FFFFFF">Nucleases characterization and cheater study : Arnaud</span></span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part933"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part933" data-index="65" style="margin-left: 0px;">Culture of A1, Moclo and A1 cheater (they survided the nuclease induction from a previous induction but not a single clone but many were used for the preculture) in LB + Amp (25mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part934"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part934" data-index="66" style="margin-left: 0px;">O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part935"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part935" data-index="67" style="margin-left: 0px;">Results :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part936"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="68" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1566" data-scroll-id="note-part937"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part937-item" data-formattable="true" data-index="69" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="69" data-printable-layout-width="865" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part937-table" data-formattable="true" data-index="69" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 857px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 845px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col><col data-id="7" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">H</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">H</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-0" data-namespace="table-ns1494"><span>O.D</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-5" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-6" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-0-7" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-0" data-namespace="table-ns1494"><span>Time Point</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-1" data-namespace="table-ns1494"><span>10h14</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-2" data-namespace="table-ns1494"><span>10h44 (-30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-3" data-namespace="table-ns1494"><span>11h14 (T0) Arabinose is added</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-4" data-namespace="table-ns1494"><span>11h44 (+30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-5" data-namespace="table-ns1494"><span>12h44 (+90')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-6" data-namespace="table-ns1494"><span>13h44 (+150')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-1-7" data-namespace="table-ns1494"><span>TOMORROW 12h15 (+1351)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-2-0" data-namespace="table-ns1494"><span>A1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-2" data-namespace="table-ns1494"><span>0.16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-3" data-namespace="table-ns1494"><span>0.32</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-4" data-namespace="table-ns1494"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-5" data-namespace="table-ns1494"><span>0.57</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-6" data-namespace="table-ns1494"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-2-7" data-namespace="table-ns1494"><span>3.00</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-3-0" data-namespace="table-ns1494"><span>Moclo</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-2" data-namespace="table-ns1494"><span>0.18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-3" data-namespace="table-ns1494"><span>0.40</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-4" data-namespace="table-ns1494"><span>0.66</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-5" data-namespace="table-ns1494"><span>1.18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-6" data-namespace="table-ns1494"><span>1.64</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-3-7" data-namespace="table-ns1494"><span>2.40</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-4-0" data-namespace="table-ns1494"><span>A1 Cheater</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-2" data-namespace="table-ns1494"><span>0.20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-3" data-namespace="table-ns1494"><span>0.42</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-4" data-namespace="table-ns1494"><span>0.69</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-5" data-namespace="table-ns1494"><span>1.20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part937-4-6" data-namespace="table-ns1494"><span>1.66</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part937-4-7" data-namespace="table-ns1494"><span>/</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">O.D Measurement</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part937" data-index="69" style="max-width: 865px; margin-left: 0px;">
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part928"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part928" data-index="55" style="margin-left: 0px;">Precultures of LB plus 10µL of ampicilline and 100µL of glucose <span style="color:#344043"><span style="background-color:#FFFFFF">(except for KEÏO Z1 and Hfr)</span></span> were used to inoculate 10 mL of each of our strains. 583 µL of preculture A1 was put into 15mL of LB, 600µL of MoClo, 588µL of T7 and 619µL of Keio Z1. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part929"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part929" data-index="56" style="margin-left: 0px;">The CACL2, MgCl2 were added after 30min of culture. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part930"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part930" data-index="57" style="margin-left: 0px;">The final OD600nm used for the infection were: </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part931"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part931" data-index="58" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1633" data-scroll-id="note-part932"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part932-item" data-formattable="true" data-index="59" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="59" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part932-table" data-formattable="true" data-index="59" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-0-0" data-namespace="table-ns1567"><span>Strains names</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-0-1" data-namespace="table-ns1567"><span>KEIO Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-0-2" data-namespace="table-ns1567"><span>MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-0-3" data-namespace="table-ns1567"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-0-4" data-namespace="table-ns1567"><span>T7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part932-1-0" data-namespace="table-ns1567"><span>OD 600nm</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part932-1-1" data-namespace="table-ns1567"><span>0.5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part932-1-2" data-namespace="table-ns1567"><span>0.35</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part932-1-3" data-namespace="table-ns1567"><span>0.42</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part932-1-4" data-namespace="table-ns1567"><span>0.32</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table1</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part932" data-index="59" style="max-width: 565px; margin-left: 0px;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1567" data-scroll-id="note-part938"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part938-item" data-formattable="true" data-index="70" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="70" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part938-table" data-formattable="true" data-index="70" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part938-0-0" data-namespace="table-ns1494"><span>CFU</span></td><td class="cell is-text is-alignCenter cell-contents" colspan="5" data-cid="table-note-part938-0-1" data-namespace="table-ns1494"><span>Time (min)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part938-1-0" data-namespace="table-ns1494"><span>Bacteria</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part938-1-1" data-namespace="table-ns1494" style="background-color: rgb(226, 239, 218);"><span>-30</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part938-1-2" data-namespace="table-ns1494" style="background-color: rgb(198, 224, 180);"><span>30</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part938-1-3" data-namespace="table-ns1494" style="background-color: rgb(169, 208, 142);"><span>90</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part938-1-4" data-namespace="table-ns1494" style="background-color: rgb(84, 130, 53);"><span>150</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part938-1-5" data-namespace="table-ns1494" style="background-color: rgb(55, 86, 35);"><span>1351</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part938-2-0" data-namespace="table-ns1494" rowspan="3" style="background-color: rgb(189, 215, 238);"><span>A1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-2-1" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-2-2" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-2</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-2-3" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-2-4" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-2-5" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-3-1" data-namespace="table-ns1494"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-3-2" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-3-3" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-3-4" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-3-5" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-4-1" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-4-2" data-namespace="table-ns1494"><span>4</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-4-3" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-4-4" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-4-5" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part938-5-0" data-namespace="table-ns1494" rowspan="3" style="background-color: rgb(255, 217, 102);"><span>Moclo</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-5-1" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-5-2" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-5-3" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-5-4" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-5-5" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-6-1" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-6-2" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-6-3" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-6-4" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-6-5" data-namespace="table-ns1494"><span>33</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-7-1" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-7-2" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-7-3" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-7-4" data-namespace="table-ns1494"><span>7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-7-5" data-namespace="table-ns1494"><span>22</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">9</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">9</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part938-8-0" data-namespace="table-ns1494" rowspan="3" style="background-color: rgb(47, 117, 181);"><span>Survivor A1 N°3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-8-1" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-5</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-8-2" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-8-3" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-8-4" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span>-6</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-8-5" data-namespace="table-ns1494" style="background-color: rgb(252, 228, 214);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">10</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">10</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-9-1" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-9-2" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-9-3" data-namespace="table-ns1494"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-9-4" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-9-5" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">11</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">11</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-10-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-10-2" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-10-3" data-namespace="table-ns1494"><span>7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part938-10-4" data-namespace="table-ns1494"><span>6</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part938-10-5" data-namespace="table-ns1494" style="background-color: rgb(192, 0, 0);"><span></span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Colony number at different time for each condition and dilution factor associated</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part938" data-index="70" style="max-width: 665px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part933"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part933" data-index="60" style="margin-left: 0px;">According to the formula from the protocol, the amount of phages used was <span style="color:#344043"><span style="background-color:#FFFFFF">with a concentration of 4.18*10^5 PFU/mL</span></span>: </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part934"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part934" data-index="61" style="margin-left: 175px;">Keio Z1 : 281 µL in 10ml medium plus bacteria</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part935"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part935" data-index="62" style="margin-left: 175px;">MoClo: 263 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part936"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="63" style="margin-left: 175px;">A1: 275 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part937"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part937" data-index="64" style="margin-left: 175px;">T7: 233 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part938"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="65" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part939"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="66" style="margin-left: 0px;">Hfr cells had an OD<sub>600nm</sub> of 0,79 when they were poured into soft Agar containing 1mM of CaCl2 and MgCl2.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part940"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part940" data-index="67" style="margin-left: 0px;">Serial dilutions started from 10<sup>-1</sup> to 10<sup>-9</sup>, 20µL of undiluted supernatant was put into 180µL of phage buffer.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part941"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part941" data-index="68" style="margin-left: 0px;">The ODs were taken for 0min, 60 min and 120 min:</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part942"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="69" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1634" data-scroll-id="note-part943"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part943-item" data-formattable="true" data-index="70" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="70" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part943-table" data-formattable="true" data-index="70" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-0-0" data-namespace="table-ns1567"><span>Strains</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-0-1" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-0-2" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-MoClo1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-0-3" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-0-4" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-T7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-1-0" data-namespace="table-ns1567"><span>T=0min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-1-1" data-namespace="table-ns1567"><span>0.28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-1-2" data-namespace="table-ns1567"><span>0.28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-1-3" data-namespace="table-ns1567"><span>0.28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-1-4" data-namespace="table-ns1567"><span>0.2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-2-0" data-namespace="table-ns1567"><span>T=60min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-2-1" data-namespace="table-ns1567"><span>0.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-2-2" data-namespace="table-ns1567"><span>0.7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-2-3" data-namespace="table-ns1567"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-2-4" data-namespace="table-ns1567"><span>0.29</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-3-0" data-namespace="table-ns1567"><span>T=120min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-3-1" data-namespace="table-ns1567"><span>1.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-3-2" data-namespace="table-ns1567"><span>0.69</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-3-3" data-namespace="table-ns1567"><span>0.55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-3-4" data-namespace="table-ns1567"><span>0.21</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part943-4-0" data-namespace="table-ns1567"><span>T= Day after</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-4-1" data-namespace="table-ns1567"><span>3.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-4-2" data-namespace="table-ns1567"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-4-3" data-namespace="table-ns1567"><span>0.9</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part943-4-4" data-namespace="table-ns1567"><span>2.16</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">OD600nm every 1 hour for MS2 infection and the day after</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part943" data-index="70" style="max-width: 565px; margin-left: 0px;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part939"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="71" style="margin-left: 0px;">Most of the results are not usable because the droplet mixed themself together</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part940"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part940" data-index="72" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part941"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part941" data-index="73" style="margin-left: 35px;"> <b><span style="color:#344043"><span style="background-color:#FFFFFF">Precultures of one clone pBAD24 Moclo, </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBAD24 T7 and 2 survivors  after induction of the nuclease A1 </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">: Clara</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part942"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="74" style="margin-left: 0px;">Each strains were put into 15mL of LB + amp (15µL) + glucose (150µL)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part943"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part943" data-index="75" style="margin-left: 0px;">The survivors/cheaters were taking from the plate 07/16 for the "day after" condition, #2 clone 5 and #1 clone4</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part944"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part944" data-index="76" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part945"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part945" data-index="77" style="margin-left: 35px;"><b>Culture of DH5alpha to<span style="color:#344043"><span style="background-color:#FFFFFF"> make ultra competent cells : Laetitia</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part946"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="78" style="margin-left: 0px;">1mL from the day before preculture (05/08/2019) has been added to 250mL of LB and let grown at 20°C, 140 rpm until D0<sub>600</sub>=0.55</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part947"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="79" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part948"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="80" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay852"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part949"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part949" data-index="81" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part950"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part950" data-index="82" style="margin-left: 35px;"><b><span style="color:#344043"><span style="color:#222222"><span style="background-color:#FFFFFF">Nucleases T7 characterization and A1</span></span></span><span style="color:#344043"><span style="color:#222222"><span style="background-color:#FFFFFF"> cheaters study : Arnaud_Clara</span></span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part951"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="83" style="margin-left: 0px;">Culture of Moclo T7 (*2) and A1 cheaters clone 4 and 5 (they survided the nuclease induction from a previous induction but not a single clone but many were used for the preculture) in LB + Amp (25mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4. O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part952"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="84" style="margin-left: 0px;">Results :</div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1568" data-scroll-id="note-part953"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part953-item" data-formattable="true" data-index="85" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="85" data-printable-layout-width="865" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part953-table" data-formattable="true" data-index="85" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 857px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 845px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col><col data-id="7" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">H</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">H</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-0" data-namespace="table-ns1494"><span>O.D.</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-5" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-6" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-0-7" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-0" data-namespace="table-ns1494"><span>Time point</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-1" data-namespace="table-ns1494"><span>11h20</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-2" data-namespace="table-ns1494"><span>11h50 (-30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-3" data-namespace="table-ns1494"><span>12h20 (T0)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-4" data-namespace="table-ns1494"><span>12h50 (+30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-5" data-namespace="table-ns1494"><span>13h50 (+90')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-6" data-namespace="table-ns1494"><span>14h50 (+150')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-1-7" data-namespace="table-ns1494"><span>TOMORROW</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-2-0" data-namespace="table-ns1494"><span>Moclo</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-2" data-namespace="table-ns1494"><span>0.29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-3" data-namespace="table-ns1494"><span>0.53</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-4" data-namespace="table-ns1494"><span>0.87</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-5" data-namespace="table-ns1494"><span>1.53</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-6" data-namespace="table-ns1494"><span>1.94</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-2-7" data-namespace="table-ns1494"><span>4.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-3-0" data-namespace="table-ns1494"><span>T7 n°1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-2" data-namespace="table-ns1494"><span>0.20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-3" data-namespace="table-ns1494"><span>0.32</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-4" data-namespace="table-ns1494"><span>0.42</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-5" data-namespace="table-ns1494"><span>0.36</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-6" data-namespace="table-ns1494"><span>0.29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-3-7" data-namespace="table-ns1494"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-4-0" data-namespace="table-ns1494"><span>T7 n°2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-2" data-namespace="table-ns1494"><span>0.25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-3" data-namespace="table-ns1494"><span>0.41</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-4" data-namespace="table-ns1494"><span>0.42</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-5" data-namespace="table-ns1494"><span>0.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-6" data-namespace="table-ns1494"><span>0.36</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-4-7" data-namespace="table-ns1494"><span>3.9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-5-0" data-namespace="table-ns1494"><span>Cheater A1 clone 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-2" data-namespace="table-ns1494"><span>0.31</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-3" data-namespace="table-ns1494"><span>0.52</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-4" data-namespace="table-ns1494"><span>0.85</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-5" data-namespace="table-ns1494"><span>1.51</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-6" data-namespace="table-ns1494"><span>1.87</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-5-7" data-namespace="table-ns1494"><span>3.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part953-6-0" data-namespace="table-ns1494"><span>Cheater A1 clone 5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-2" data-namespace="table-ns1494"><span>0.29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-3" data-namespace="table-ns1494"><span>0.47</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-4" data-namespace="table-ns1494"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-5" data-namespace="table-ns1494"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-6" data-namespace="table-ns1494"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part953-6-7" data-namespace="table-ns1494"><span>3.4</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table1</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part953" data-index="85" style="max-width: 865px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part944"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part944" data-index="71" style="margin-left: 0px;">HFR stayed all night long on table and then mixed with soft agar for the last time point (day after). </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part945"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part945" data-index="72" style="margin-left: 0px;">Bacteria and phages mix wasn't put at 37°C in LB + ARA directly, stayed in ice </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part946"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="73" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part947"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="74" style="margin-left: 0px;">Results of the infection assay :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part948"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="75" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part949"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part949-item" data-formattable="true" data-index="76" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Phage titration &amp; infection 01082019- corrected Laetitia.xlsx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part949" data-index="76">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part954"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="86" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1569" data-scroll-id="note-part955"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part955-item" data-formattable="true" data-index="87" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="87" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part955-table" data-formattable="true" data-index="87" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-0" data-namespace="table-ns1494"><span>CFU</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-1" data-namespace="table-ns1494"><span>Time (min)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-0-5" data-namespace="table-ns1494"><span> </span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-1-0" data-namespace="table-ns1494"><span>Bacteria</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-1-1" data-namespace="table-ns1494"><span>-30</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-1-2" data-namespace="table-ns1494"><span>30</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-1-3" data-namespace="table-ns1494"><span>90</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-1-4" data-namespace="table-ns1494"><span>150</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-1-5" data-namespace="table-ns1494"><span>TOMORROW</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-2-0" data-namespace="table-ns1494"><span>Moclo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-2-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-2-2" data-namespace="table-ns1494"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-2-3" data-namespace="table-ns1494"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-2-4" data-namespace="table-ns1494"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-2-5" data-namespace="table-ns1494"><span>-5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-3-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-3-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-3-2" data-namespace="table-ns1494"><span>27</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-3-3" data-namespace="table-ns1494"><span>26</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-3-4" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-3-5" data-namespace="table-ns1494"><span>27</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-4-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-4-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-4-2" data-namespace="table-ns1494"><span>26</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-4-3" data-namespace="table-ns1494"><span>33</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-4-4" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-4-5" data-namespace="table-ns1494"><span>26</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-5-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-5-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-5-2" data-namespace="table-ns1494"><span>27</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-5-3" data-namespace="table-ns1494"><span>29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-5-4" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-5-5" data-namespace="table-ns1494"><span>27</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-6-0" data-namespace="table-ns1494"><span>T7 N°1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-6-1" data-namespace="table-ns1494"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-6-2" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-6-3" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-6-4" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-6-5" data-namespace="table-ns1494"><span>-5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-7-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-7-1" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-7-2" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-7-3" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-7-4" data-namespace="table-ns1494"><span>21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-7-5" data-namespace="table-ns1494"><span>7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">9</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">9</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-8-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-8-1" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-8-2" data-namespace="table-ns1494"><span>7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-8-3" data-namespace="table-ns1494"><span>6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-8-4" data-namespace="table-ns1494"><span>29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-8-5" data-namespace="table-ns1494"><span>4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">10</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">10</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-9-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-9-1" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-9-2" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-9-3" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-9-4" data-namespace="table-ns1494"><span>22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-9-5" data-namespace="table-ns1494"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">11</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">11</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-10-0" data-namespace="table-ns1494"><span>T7 N°2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-10-1" data-namespace="table-ns1494"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-10-2" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-10-3" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-10-4" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-10-5" data-namespace="table-ns1494"><span>-3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">12</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">12</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-11-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-11-1" data-namespace="table-ns1494"><span>18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-11-2" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-11-3" data-namespace="table-ns1494"><span>17</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-11-4" data-namespace="table-ns1494"><span>25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-11-5" data-namespace="table-ns1494"><span>16</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">13</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">13</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-12-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-12-1" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-12-2" data-namespace="table-ns1494"><span>18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-12-3" data-namespace="table-ns1494"><span>25</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-12-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-12-5" data-namespace="table-ns1494"><span>18</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">14</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">14</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-13-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-13-1" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-13-2" data-namespace="table-ns1494"><span>20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-13-3" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-13-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-13-5" data-namespace="table-ns1494"><span>11</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">15</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">15</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-14-0" data-namespace="table-ns1494"><span>Survivor A1 N° 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-14-1" data-namespace="table-ns1494"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-14-2" data-namespace="table-ns1494"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-14-3" data-namespace="table-ns1494"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-14-4" data-namespace="table-ns1494"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-14-5" data-namespace="table-ns1494"><span>-5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">16</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">16</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-15-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-15-1" data-namespace="table-ns1494"><span>22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-15-2" data-namespace="table-ns1494"><span>21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-15-3" data-namespace="table-ns1494"><span>17</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-15-4" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-15-5" data-namespace="table-ns1494"><span>20</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">17</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">17</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-16-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-16-1" data-namespace="table-ns1494"><span>32</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-16-2" data-namespace="table-ns1494"><span>28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-16-3" data-namespace="table-ns1494"><span>17</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-16-4" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-16-5" data-namespace="table-ns1494"><span>34</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">18</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">18</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-17-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-17-1" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-17-2" data-namespace="table-ns1494"><span>22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-17-3" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-17-4" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-17-5" data-namespace="table-ns1494"><span>28</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">19</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">19</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-18-0" data-namespace="table-ns1494"><span>Survivor A1 N° 5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-18-1" data-namespace="table-ns1494"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-18-2" data-namespace="table-ns1494"><span>-2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-18-3" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-18-4" data-namespace="table-ns1494"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-18-5" data-namespace="table-ns1494"><span>-2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">20</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">20</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-19-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-19-1" data-namespace="table-ns1494"><span>26</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-19-2" data-namespace="table-ns1494"><span>22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-19-3" data-namespace="table-ns1494"><span>17</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-19-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-19-5" data-namespace="table-ns1494"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">21</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">21</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-20-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-20-1" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-20-2" data-namespace="table-ns1494"><span>26</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-20-3" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-20-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-20-5" data-namespace="table-ns1494"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">22</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">22</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-21-0" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-21-1" data-namespace="table-ns1494"><span>18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-21-2" data-namespace="table-ns1494"><span>7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-21-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part955-21-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part955-21-5" data-namespace="table-ns1494"><span>4</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">CFU</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part955" data-index="87" style="max-width: 665px; margin-left: 0px;">
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part951"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="77" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part952"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="78" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay852"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 2/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part953"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part953" data-index="79" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part954"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="80" style="margin-left: 35px;"><b><span style="color:#344043"><span style="background-color:#FFFFFF">Phage infection (Clara_Arnaud)</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part955"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part955" data-index="81" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part956"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part956" data-index="82" style="margin-left: 0px;">Results: the point "day after" wasn't good, couldn't count anything, maybe because of the HFR stayed all night long on the bench. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part957"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="83" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay854"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part958"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part958" data-index="84" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part959"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part959" data-index="85" style="margin-left: 0px;">See the protocol "Infection assay" </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part960"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="86" style="margin-left: 0px;">Precultures of LB plus 20µL of ampicilline and 200µL of glucose (except for KEÏO Z1 and Hfr)  were used to inoculate 20 mL of each of our strains. 769 µL of preculture A1 and MoClo was put into 15mL of LB, 741µL of T7 and 667µL of Keio Z1. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part961"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part961" data-index="87" style="margin-left: 0px;">The CACL2, MgCl2 were added at a final concentration of 1mM. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part962"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part962" data-index="88" style="margin-left: 0px;">The final OD600nm used for the infection were: </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part963"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="89" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1635" data-scroll-id="note-part964"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part964-item" data-formattable="true" data-index="90" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="90" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part964-table" data-formattable="true" data-index="90" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-0-0" data-namespace="table-ns1567"><span>Strains name</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-0-1" data-namespace="table-ns1567"><span>KEIO Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-0-2" data-namespace="table-ns1567"><span>MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-0-3" data-namespace="table-ns1567"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-0-4" data-namespace="table-ns1567"><span>T7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-1-0" data-namespace="table-ns1567"><span>OD 600nm</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-1-1" data-namespace="table-ns1567"><span>0,41</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-1-2" data-namespace="table-ns1567"><span>0,49</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-1-3" data-namespace="table-ns1567"><span>0,42</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part964-1-4" data-namespace="table-ns1567"><span>0,38</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table2</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part964" data-index="90" style="max-width: 565px; margin-left: 0px;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part956"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part956" data-index="88" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay854"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part957"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="89" style="margin-left: 35px;"><b>Plasmid extraction : Arnaud</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part958"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part958" data-index="90" style="margin-left: 0px;">Extraction of plasmids from : </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part959"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 70px;"><span>a.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part959" data-index="91" style="margin-left: 70px;">Cheater (survivor of previous nuclease induction) A1 clone 1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part960"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 70px;"><span>b.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="92" style="margin-left: 70px;">Cheater A1 clone 4 </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part961"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 70px;"><span>c.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part961" data-index="93" style="margin-left: 70px;">Cheater T7 clone 1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part962"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part962" data-index="94" style="margin-left: 0px;">Use of Macherey-Nagel kit "DNA and RNA purification" protocol page 18 "Low copy"</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part963"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="95" style="margin-left: 0px;">Analysis with Nanodrop :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part964"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part964" data-index="96" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1570" data-scroll-id="note-part965"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part965-item" data-formattable="true" data-index="97" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="97" data-printable-layout-width="265" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part965-table" data-formattable="true" data-index="97" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 257px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 245px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part965-0-0" data-namespace="table-ns1494"><span>Plasmid from</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part965-0-1" data-namespace="table-ns1494"><span>Concentration (ng/µL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part965-1-0" data-namespace="table-ns1494"><span>Cheater A1 clone 1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part965-1-1" data-namespace="table-ns1494"><span>149</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part965-2-0" data-namespace="table-ns1494"><span>Cheater A1 clone 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part965-2-1" data-namespace="table-ns1494"><span>169</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part965-3-0" data-namespace="table-ns1494"><span>Cheater T7 clone 1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part965-3-1" data-namespace="table-ns1494"><span>184</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Nanodrop Results 9/08</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part965" data-index="97" style="max-width: 265px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part965"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part965" data-index="91" style="margin-left: 0px;">According to the formula from the protocol, the amount of phages used was with a concentration of 4.18*10^5 PFU/mL: </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part966"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="92" style="margin-left: 175px;">Keio Z1 : 294 µL in 20ml medium plus bacteria</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part967"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="93" style="margin-left: 175px;">MoClo: 352 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part968"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="94" style="margin-left: 175px;">A1: 301 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part969"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="95" style="margin-left: 175px;">T7: 273 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part970"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="96" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part971"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="97" style="margin-left: 0px;"><span style="color:#000000">After 1hour in ice, LB + 0,2% arabinose and 100 µg/µL of ampicilline was added to the mix of phages and bacteria. </span><b><span style="color:#D10E0E">Normally, the strain KEIO Z1 shouldn't have received Ampicilline since it is sensitive to it. </span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part972"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part972" data-index="98" style="margin-left: 0px;">Hfr cells had an OD<sub>600nm</sub> of 0,67 when they were poured into soft Agar containing 1mM of CaCl2 and MgCl2.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part973"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part973" data-index="99" style="margin-left: 0px;">Serial dilutions started from 10<sup>-1</sup> to 10<sup>-9</sup>, 20µL of undiluted supernatant was put into 180µL of phage buffer.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part974"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part974" data-index="100" style="margin-left: 0px;">The ODs were taken for 0min, 20min, 40min,60 min, 80 min, 100 min and 120 min:</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part975"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part975" data-index="101" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1636" data-scroll-id="note-part976"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part976-item" data-formattable="true" data-index="102" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="102" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part976-table" data-formattable="true" data-index="102" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-0-0" data-namespace="table-ns1567"><span>Strains</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-0-1" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-0-2" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-MoClo1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-0-3" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-0-4" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-T7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-1-0" data-namespace="table-ns1567"><span>T=-60min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-1-1" data-namespace="table-ns1567"><span>0.41</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-1-2" data-namespace="table-ns1567"><span>0.49</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-1-3" data-namespace="table-ns1567"><span>0.42</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-1-4" data-namespace="table-ns1567"><span>0.38</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-2-0" data-namespace="table-ns1567"><span>T=20 min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-2-1" data-namespace="table-ns1567"><span>0.54</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-2-2" data-namespace="table-ns1567"><span>0.64</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-2-3" data-namespace="table-ns1567"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-2-4" data-namespace="table-ns1567"><span>0.33</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-3-0" data-namespace="table-ns1567"><span>T=40min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-3-1" data-namespace="table-ns1567"><span>0.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-3-2" data-namespace="table-ns1567"><span>0.87</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-3-3" data-namespace="table-ns1567"><span>0.56</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-3-4" data-namespace="table-ns1567"><span>0.23</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-4-0" data-namespace="table-ns1567"><span>T=60min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-4-1" data-namespace="table-ns1567"><span>0.25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-4-2" data-namespace="table-ns1567"><span>0.94</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-4-3" data-namespace="table-ns1567"><span>0.55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-4-4" data-namespace="table-ns1567"><span>0.15</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-5-0" data-namespace="table-ns1567"><span>T=80min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-5-1" data-namespace="table-ns1567"><span>0.22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-5-2" data-namespace="table-ns1567"><span>0.5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-5-3" data-namespace="table-ns1567"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-5-4" data-namespace="table-ns1567"><span>0.15</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-6-0" data-namespace="table-ns1567"><span>T=100min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-6-1" data-namespace="table-ns1567"><span>0.15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-6-2" data-namespace="table-ns1567"><span>0.27</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-6-3" data-namespace="table-ns1567"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-6-4" data-namespace="table-ns1567"><span>0.11</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part976-7-0" data-namespace="table-ns1567"><span>T=120min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-7-1" data-namespace="table-ns1567"><span>0.14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-7-2" data-namespace="table-ns1567"><span>0.23</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-7-3" data-namespace="table-ns1567"><span>0.57</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part976-7-4" data-namespace="table-ns1567"><span>0.1</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table3</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part976" data-index="102" style="max-width: 565px; margin-left: 0px;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part966"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="98" style="margin-left: 35px;"><b>Heatshock plasmid Transformation : Arnaud</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part967"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="99" style="margin-left: 35px;">5 transformations are done : 2 are double transformations and the other are simple transformation. 1 µL per plasmid and 50 µL of competent KeioZ1F' :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part968"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"><span>I.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="100" style="margin-left: 105px;">KeioZ1F' with plasmid from cheater A1 clone 4 (previously extracted)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part969"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"><span>II.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="101" style="margin-left: 105px;">KeioZ1F' with plasmid from cheater T7 clone 1 (previously extracted)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part970"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="102" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part971"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="103" style="margin-left: 35px;"><b>Preculture : Arnaud</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part972"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part972" data-index="104" style="margin-left: 0px;"><span style="color:#344043"><span style="background-color:#FFFFFF">Preculture of KeioZ1F'_A1, Moclo, T7, B.sub, KeioZ1F' transformed with plasmid from Cheater A1 clone 4 and KeioZ1F' transformed with plasmid from Cheater T7 clone 1 3 mL LB + Ampiciline + Glucose 0.2% overnight with shaking at 37°C.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part973"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part973" data-index="105" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay856"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 12/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part974"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part974" data-index="106" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part975"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part975" data-index="107" style="margin-left: 35px;"><b><span style="color:#344043"><span style="color:#222222"><span style="background-color:#FFFFFF">Nucleases caracterization and cheater study : Arnaud</span></span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part976"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part976" data-index="108" style="margin-left: 0px;">Culture of A1, Moclo, T7 , B.sub, <span style="color:#344043"><span style="background-color:#FFFFFF">KeioZ1F' transformed with plasmid from Cheater A1 clone 4 and KeioZ1F' transformed with plasmid from Cheater T7 clone 1 </span></span>in LB + Amp (50mL) after a preculture overnight in LB + amp + glucose. Liquide preculture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction by adding arabinose when O.D is around 0.4. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part977"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part977" data-index="109" style="margin-left: 0px;">O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (around +1110)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part978"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part978" data-index="110" style="margin-left: 0px;">Arabinose added at 12h10 (Cf = 0.2%)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part979"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part979" data-index="111" style="margin-left: 0px;">Results :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part980"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part980" data-index="112" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1571" data-scroll-id="note-part981"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part981-item" data-formattable="true" data-index="113" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="113" data-printable-layout-width="865" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part981-table" data-formattable="true" data-index="113" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 857px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 845px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col><col data-id="7" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">H</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">H</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-0" data-namespace="table-ns1494"><span>Time point</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-1" data-namespace="table-ns1494"><span>10h55</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-2" data-namespace="table-ns1494"><span>11h30 (t-30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-3" data-namespace="table-ns1494"><span>12h00 (t0)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-4" data-namespace="table-ns1494"><span>12h40 (t+30')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-5" data-namespace="table-ns1494"><span>13h40 (t+90')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-6" data-namespace="table-ns1494"><span>14h40 (t+150')</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-0-7" data-namespace="table-ns1494"><span>Tomorrow 11h15 (t+1395')</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-1-0" data-namespace="table-ns1494"><span>A1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-2" data-namespace="table-ns1494"><span>0.18</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-3" data-namespace="table-ns1494"><span>0.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-4" data-namespace="table-ns1494"><span>0.55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-5" data-namespace="table-ns1494"><span>0.51</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-6" data-namespace="table-ns1494"><span>0.55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-1-7" data-namespace="table-ns1494"><span>3.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-2-0" data-namespace="table-ns1494"><span>T7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-2" data-namespace="table-ns1494"><span>0.15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-3" data-namespace="table-ns1494"><span>0.17</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-4" data-namespace="table-ns1494"><span>0.20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-5" data-namespace="table-ns1494"><span>0.20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-6" data-namespace="table-ns1494"><span>0.17</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-2-7" data-namespace="table-ns1494"><span>3.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-3-0" data-namespace="table-ns1494"><span>Moclo</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-2" data-namespace="table-ns1494"><span>0.19</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-3" data-namespace="table-ns1494"><span>0.34</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-4" data-namespace="table-ns1494"><span>0.71</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-5" data-namespace="table-ns1494"><span>1.31</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-6" data-namespace="table-ns1494"><span>1.83</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-3-7" data-namespace="table-ns1494"><span>3.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-4-0" data-namespace="table-ns1494"><span>B.sub</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-2" data-namespace="table-ns1494"><span>0.13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-3" data-namespace="table-ns1494"><span>0.21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-4" data-namespace="table-ns1494"><span>0.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-5" data-namespace="table-ns1494"><span>0.61</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-6" data-namespace="table-ns1494"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-4-7" data-namespace="table-ns1494"><span>4.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-5-0" data-namespace="table-ns1494"><span>KeioZ1F' transformed with pasmid from Cheater A1 clone 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-2" data-namespace="table-ns1494"><span>0.21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-3" data-namespace="table-ns1494"><span>0.40</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-4" data-namespace="table-ns1494"><span>0.68</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-5" data-namespace="table-ns1494"><span>1.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-6" data-namespace="table-ns1494"><span>1.80</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-5-7" data-namespace="table-ns1494"><span>3.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part981-6-0" data-namespace="table-ns1494"><span>KeioZ1F' transformed with pasmid from Cheater T7 clone 1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-1" data-namespace="table-ns1494"><span>0.1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-2" data-namespace="table-ns1494"><span>0.16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-3" data-namespace="table-ns1494"><span>0.35</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-4" data-namespace="table-ns1494"><span>0.63</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-5" data-namespace="table-ns1494"><span>1.23</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-6" data-namespace="table-ns1494"><span>1.75</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part981-6-7" data-namespace="table-ns1494"><span>3.3</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">O.D. 12/08</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part981" data-index="113" style="max-width: 865px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part977"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part977" data-index="103" style="margin-left: 0px;"><b><u>Results of the infection assay :</u></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part978"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part978" data-index="104" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part979"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part979-item" data-formattable="true" data-index="105" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Phage titration &amp; infection.xlsx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part979" data-index="105">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1572" data-scroll-id="note-part982"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part982-item" data-formattable="true" data-index="114" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="114" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part982-table" data-formattable="true" data-index="114" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-0-0" data-namespace="table-ns1494"><span>CFU</span></td><td class="cell is-text is-alignCenter cell-contents" colspan="5" data-cid="table-note-part982-0-1" data-namespace="table-ns1494"><span>Time (min)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-1-0" data-namespace="table-ns1494"><span>Bacteria</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part982-1-1" data-namespace="table-ns1494" style="background-color: rgb(226, 239, 218);"><span>-30</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part982-1-2" data-namespace="table-ns1494" style="background-color: rgb(198, 224, 180);"><span>30</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part982-1-3" data-namespace="table-ns1494" style="background-color: rgb(169, 208, 142);"><span>90</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part982-1-4" data-namespace="table-ns1494" style="background-color: rgb(84, 130, 53);"><span>150</span></td><td class="cell is-value is-alignCenter cell-contents" data-cid="table-note-part982-1-5" data-namespace="table-ns1494" style="background-color: rgb(55, 86, 35);"><span>1395</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-2-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(189, 215, 238);"><span>Moclo</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-2-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-2-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-2-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-2-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-2-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-3-1" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-3-2" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-3-3" data-namespace="table-ns1494"><span>27</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-3-4" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-3-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-4-1" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-4-2" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-4-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-4-4" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-4-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-5-1" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-5-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-5-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-5-4" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-5-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-6-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(255, 230, 153);"><span>B.sub</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-6-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-6-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-6-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-6-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-2</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-6-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-7-1" data-namespace="table-ns1494"><span>20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-7-2" data-namespace="table-ns1494"><span>25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-7-3" data-namespace="table-ns1494"><span>11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-7-4" data-namespace="table-ns1494"><span>31</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-7-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">9</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">9</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-8-1" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-8-2" data-namespace="table-ns1494"><span>30</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-8-3" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-8-4" data-namespace="table-ns1494"><span>30</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-8-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">10</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">10</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-9-1" data-namespace="table-ns1494"><span>21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-9-2" data-namespace="table-ns1494"><span>28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-9-3" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-9-4" data-namespace="table-ns1494"><span>24</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-9-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">11</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">11</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-10-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(191, 143, 0);"><span>T7</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-10-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-10-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-10-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-10-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-2</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-10-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">12</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">12</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-11-1" data-namespace="table-ns1494"><span>4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-11-2" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-11-3" data-namespace="table-ns1494"><span>No colony after</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-11-4" data-namespace="table-ns1494"><span>2</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-11-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">13</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">13</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-12-1" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-12-2" data-namespace="table-ns1494"><span>20</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-12-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-12-4" data-namespace="table-ns1494"><span>4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-12-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">14</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">14</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-13-1" data-namespace="table-ns1494"><span>5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-13-2" data-namespace="table-ns1494"><span>19</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-13-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-13-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-13-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">15</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">15</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-14-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(48, 84, 150);"><span>A1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-14-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-14-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-14-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-14-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-14-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">16</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">16</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-15-1" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-15-2" data-namespace="table-ns1494"><span>No colony after</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-15-3" data-namespace="table-ns1494"><span>No colony after</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-15-4" data-namespace="table-ns1494"><span>No colony after</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-15-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">17</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">17</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-16-1" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-16-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-16-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-16-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-16-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">18</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">18</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-17-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-17-2" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-17-3" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-17-4" data-namespace="table-ns1494"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-17-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">19</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">19</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignCenter cell-contents" data-cid="table-note-part982-18-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(198, 89, 17);"><span>KeioZ1F' transformed with pasmid from Cheater A1 clone 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-18-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-18-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-18-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-18-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-18-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">20</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">20</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-19-1" data-namespace="table-ns1494"><span>10</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-19-2" data-namespace="table-ns1494"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-19-3" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>28</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-19-4" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-19-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">21</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">21</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-20-1" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-20-2" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-20-3" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-20-4" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>15</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-20-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">22</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">22</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-21-1" data-namespace="table-ns1494"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-21-2" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-21-3" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-21-4" data-namespace="table-ns1494" style="background-color: rgb(255, 255, 255);"><span>11</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-21-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">23</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">23</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-22-0" data-namespace="table-ns1494" rowspan="4" style="background-color: rgb(112, 48, 160);"><span>KeioZ1F' transformed with pasmid from Cheater T7 clone 1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-22-1" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-22-2" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-22-3" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-22-4" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span>-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-22-5" data-namespace="table-ns1494" style="background-color: rgb(248, 203, 173);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">24</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">24</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-23-1" data-namespace="table-ns1494"><span>15</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-23-2" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-23-3" data-namespace="table-ns1494"><span>25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-23-4" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-23-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">25</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">25</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-24-1" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-24-2" data-namespace="table-ns1494"><span>14</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-24-3" data-namespace="table-ns1494"><span>29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-24-4" data-namespace="table-ns1494"><span>9</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-24-5" data-namespace="table-ns1494"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">26</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">26</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-25-1" data-namespace="table-ns1494"><span>20</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-25-2" data-namespace="table-ns1494"><span>13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-25-3" data-namespace="table-ns1494"><span>16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part982-25-4" data-namespace="table-ns1494"><span>8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part982-25-5" data-namespace="table-ns1494"><span></span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">CFU 12/08</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part981"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part981" data-index="106" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part982"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part982" data-index="107" style="margin-left: 0px;">For the ODs 600nm, these results weren't expected, we don't know what went wrong --&gt; look for it.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part983"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part983" data-index="108" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part984"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part984" data-index="109" style="margin-left: 35px;"><b>Titration of Ms2 phage (Laetitia)</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part985"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part985" data-index="110" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part986"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part986" data-index="111" style="margin-left: 0px;">Phage has been titrated by folowing Titration phage protocol.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part987"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part987" data-index="112" style="margin-left: 0px;">100 <span style="color:#344043"><span style="background-color:#FFFFFF">μ</span></span>L of a 1 mL  10<sup>-5</sup> to 10<sup>-9 </sup>dilution has been plated.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part988"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part988" data-index="113" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part989"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part989" data-index="114" style="margin-left: 0px;">Results : </div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1637" data-scroll-id="note-part990"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part990-item" data-formattable="true" data-index="115" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="115" data-printable-layout-width="765" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part990-table" data-formattable="true" data-index="115" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 757px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 745px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-1" data-namespace="table-ns1567"><span>10-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-2" data-namespace="table-ns1567"><span>10-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-3" data-namespace="table-ns1567"><span>10-7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-4" data-namespace="table-ns1567"><span>10-8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-5" data-namespace="table-ns1567"><span>10-9</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-0-6" data-namespace="table-ns1567"><span>Phage titration</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-1-0" data-namespace="table-ns1567"><span>Phage already titrated at 4.10^10 phage/mL</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-1-1" data-namespace="table-ns1567"><span>non exploitable</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-1-2" data-namespace="table-ns1567"><span>confluent</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part990-1-3" data-namespace="table-ns1567"><span>88</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part990-1-4" data-namespace="table-ns1567"><span>12</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part990-1-5" data-namespace="table-ns1567"><span>4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-1-6" data-namespace="table-ns1567"><span>8,8.10^9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-0" data-namespace="table-ns1567"><span>Phage with unknown titration</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-1" data-namespace="table-ns1567"><span>Confluent</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-2" data-namespace="table-ns1567"><span>Not down</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-3" data-namespace="table-ns1567"><span>Not down</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part990-2-4" data-namespace="table-ns1567"><span>8</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-5" data-namespace="table-ns1567"><span>Not down</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part990-2-6" data-namespace="table-ns1567"><span>8.10^9 ?</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table4</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part990" data-index="115" style="max-width: 765px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="daySeparator" data-scroll-id="entryDay856"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part991"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part991" data-index="116" style="margin-left: 35px;"><b>Glycerol stock of Hfr and KeioZ1 : Arnaud</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part992"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part992" data-index="117" style="margin-left: 0px;">Glycerol stock 0.5ml (333.4 µL bacterial culture and 166.6 µL Glycerol 60%)</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part993"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part993" data-index="118" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part994"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part994" data-index="119" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay858"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 13/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part995"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part995" data-index="120" style="margin-left: 35px;"><b>Preparation of the experiment (Clara)</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part996"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part996" data-index="121" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part997"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part997" data-index="122" style="margin-left: 0px;">220 mL of soft agar without MgCl2 and CaCl2 was prepared and put at 60°C overnight</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part998"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part998" data-index="123" style="margin-left: 0px;">Hfr and KeïoZ1 strains were precultured into 3ml of LB.  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part999"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part999" data-index="124" style="margin-left: 0px;">Keïo Z1 F' cells containing the following plasmids were precultured into 3 mL of LB plus ampicilline (Cf=100µg/µm) and 0.2% glucose </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1000"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1000" data-index="125" style="margin-left: 105px;">-pBAD-A1</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1001"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1001" data-index="126" style="margin-left: 105px;">-pBAD-T7</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1002"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1002" data-index="127" style="margin-left: 105px;">-pBAD-B.subtilis</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1003"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1003" data-index="128" style="margin-left: 105px;">-pBAD-MoClo  		</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1004"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1004" data-index="129" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1005"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1005" data-index="130" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay860"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 14/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1006"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1006" data-index="131" style="margin-left: 0px;"> </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1007"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1007" data-index="132" style="margin-left: 0px;">The soft agar which was made the day before had a little thing inside, then we were suspicious about contamination. So we made another one. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1008"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1008" data-index="133" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1009"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1009" data-index="134" style="margin-left: 0px;">See the protocol "Infection assay" for more precise information.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1010"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1010" data-index="135" style="margin-left: 0px;">Precultures of LB plus 20µL of ampicilline and 200µL of glucose (except for KEÏO Z1 and Hfr)  were used to inoculate 20 mL of each of our strains. 541 µL of preculture A1 and 435 µL of preculture MoClo , 741µL of T7,  667µL of Keio Z1 and 667 µL of <i>B. subtilis</i> <span style="color:#344043"><span style="background-color:#FFFFFF">were put into 20mL of LB.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1011"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1011" data-index="136" style="margin-left: 0px;">The CACL2, MgCl2 were added at a final concentration of 1mM. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1012"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1012" data-index="137" style="margin-left: 0px;">The final OD600nm used for the infection were: </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1013"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1013" data-index="138" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1638" data-scroll-id="note-part1014"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1014-item" data-formattable="true" data-index="139" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="139" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1014-table" data-formattable="true" data-index="139" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-0-0" data-namespace="table-ns1567"><span>Strains name</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-0-1" data-namespace="table-ns1567"><span>KEIO Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-0-2" data-namespace="table-ns1567"><span>MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-0-3" data-namespace="table-ns1567"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-0-4" data-namespace="table-ns1567"><span>T7</span></td><td class="cell is-text is-italic is-alignLeft cell-contents" data-cid="table-note-part1014-0-5" data-namespace="table-ns1567"><span>B. subtilis</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-1-0" data-namespace="table-ns1567"><span>OD 600nm</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-1-1" data-namespace="table-ns1567"><span>0,4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-1-2" data-namespace="table-ns1567"><span>0,48</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-1-3" data-namespace="table-ns1567"><span>0,50</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1014-1-4" data-namespace="table-ns1567"><span>0,34</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1014-1-5" data-namespace="table-ns1567"><span>0.26</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table5</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1014" data-index="139" style="max-width: 665px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1015"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1015" data-index="140" style="margin-left: 0px;">According to the formula from the protocol, the amount of phages used was for a concentration of 8.8*10^9 PFU/mL : </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1016"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1016" data-index="141" style="margin-left: 175px;">Keio Z1 : 409 µL in 20ml medium plus bacteria</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1017"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1017" data-index="142" style="margin-left: 175px;">MoClo: 464 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1018"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1018" data-index="143" style="margin-left: 175px;">A1: 511 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1019"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1019" data-index="144" style="margin-left: 175px;">T7: 348 µL</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1020"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1020" data-index="145" style="margin-left: 175px;"><i><span style="color:#344043"><span style="background-color:#FFFFFF">B. subtilis: </span></span></i><span style="color:#344043"><span style="background-color:#FFFFFF">266 µL</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1021"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1021" data-index="146" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1022"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1022" data-index="147" style="margin-left: 0px;"><span style="color:#000000">After 30 min in ice, LB + 0,2% arabinose </span><span style="color:#000000"><span style="background-color:#FFFFFF">+ 100 µg/µL</span></span><span style="color:#000000"> was added to the mix of phages and bacteria. </span><b><span style="color:#344043"><span style="color:#D10E0E"><span style="background-color:#FFFFFF">Normally, the strain KEIO Z1 shouldn't have received Ampicilline since it is sensitive to it.</span></span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1023"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1023" data-index="148" style="margin-left: 0px;">Hfr cells had an OD<sub>600nm</sub> of 0.42 when they were poured into soft Agar containing 1mM of CaCl2 and MgCl2.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1024"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1024" data-index="149" style="margin-left: 0px;">Serial dilutions started from 10<sup>-1</sup> to 10<sup>-9</sup>, 20µL of undiluted supernatant was put into 180µL of phage buffer. In the same time, 400 µL of the remaining supernatant was put into another tube with 4 µL of chloroform in order to spread it on another plate. This way, we can spread more than a 5 µL drop and get better results for PFU/mL. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1025"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1025" data-index="150" style="margin-left: 0px;">The ODs were taken for 0min, 30 min, 60min, 90 min and 120 min:</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1026"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1026" data-index="151" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1639" data-scroll-id="note-part1027"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1027-item" data-formattable="true" data-index="152" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="152" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1027-table" data-formattable="true" data-index="152" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-0" data-namespace="table-ns1567"><span>Strains</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-1" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-2" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-MoClo1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-3" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-4" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-0-5" data-namespace="table-ns1567"><span>OD 600nm Keïo Z1 F' pBAD24-B. subtilis</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-1-0" data-namespace="table-ns1567"><span>T=0min</span></td><td class="cell is-text is-alignCenter cell-contents" colspan="1" data-cid="table-note-part1027-1-1" data-namespace="table-ns1567" rowspan="5"><span>Unexploitable results</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-1-2" data-namespace="table-ns1567"><span>0.29</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-1-3" data-namespace="table-ns1567"><span>0.34</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-1-4" data-namespace="table-ns1567"><span>0.22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-1-5" data-namespace="table-ns1567"><span>0.18</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-2-0" data-namespace="table-ns1567"><span>T=30 min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-2-2" data-namespace="table-ns1567"><span>0.66</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-2-3" data-namespace="table-ns1567"><span>0.59</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-2-4" data-namespace="table-ns1567"><span>0.22</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-2-5" data-namespace="table-ns1567"><span>0.29</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-3-0" data-namespace="table-ns1567"><span>T=60min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-3-2" data-namespace="table-ns1567"><span>0.85</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-3-3" data-namespace="table-ns1567"><span>0.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-3-4" data-namespace="table-ns1567"><span>0.16</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-3-5" data-namespace="table-ns1567"><span>0.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-4-0" data-namespace="table-ns1567"><span>T=90min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-4-2" data-namespace="table-ns1567"><span>0.31</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-4-3" data-namespace="table-ns1567"><span>0.58</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-4-4" data-namespace="table-ns1567"><span>0.11</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-4-5" data-namespace="table-ns1567"><span>0.26</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1027-5-0" data-namespace="table-ns1567"><span>T=120min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-5-2" data-namespace="table-ns1567"><span>0.31</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-5-3" data-namespace="table-ns1567"><span>0.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-5-4" data-namespace="table-ns1567"><span>0.13</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1027-5-5" data-namespace="table-ns1567"><span>0.25</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table6</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1027" data-index="152" style="max-width: 665px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1028"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1028" data-index="153" style="margin-left: 0px;"><b><u>Results of the infection assay :</u></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1029"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1029" data-index="154" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1640" data-scroll-id="note-part1030"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1030-item" data-formattable="true" data-index="155" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="155" data-printable-layout-width="665" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1030-table" data-formattable="true" data-index="155" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 657px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 645px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-0-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignCenter cell-contents" colspan="5" data-cid="table-note-part1030-0-1" data-namespace="table-ns1567" rowspan="1"><span>Time (min)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-1-0" data-namespace="table-ns1567"><span>Strain name </span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1030-1-1" data-namespace="table-ns1567"><span>0</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1030-1-2" data-namespace="table-ns1567"><span>30</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1030-1-3" data-namespace="table-ns1567"><span>60</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1030-1-4" data-namespace="table-ns1567"><span>90</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1030-1-5" data-namespace="table-ns1567"><span>120</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-0" data-namespace="table-ns1567"><span>MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-1" data-namespace="table-ns1567"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-2" data-namespace="table-ns1567"><span>10-4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-3" data-namespace="table-ns1567"><span>10^-7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-4" data-namespace="table-ns1567"><span>10^-7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-2-5" data-namespace="table-ns1567"><span>10^-7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-0" data-namespace="table-ns1567"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-1" data-namespace="table-ns1567"><span>10-4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-2" data-namespace="table-ns1567"><span>10-4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-3" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-4" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-3-5" data-namespace="table-ns1567"><span>10^-5/ 10^-6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-0" data-namespace="table-ns1567"><span>T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-1" data-namespace="table-ns1567"><span>10-4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-2" data-namespace="table-ns1567"><span>10-4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-3" data-namespace="table-ns1567"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-4" data-namespace="table-ns1567"><span>10^-5/ 10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-4-5" data-namespace="table-ns1567"><span> 10^-6/10^-7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-0" data-namespace="table-ns1567"><span>B.subtilis</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-1" data-namespace="table-ns1567"><span>10-4/10-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-2" data-namespace="table-ns1567"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-3" data-namespace="table-ns1567"><span>10^-7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-4" data-namespace="table-ns1567"><span>10^-5/ 10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1030-5-5" data-namespace="table-ns1567"><span>10^-5/ 10^-6</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Dilutions where we can count lysis ranges</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1030" data-index="155" style="max-width: 665px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1031"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1031" data-index="156" style="margin-left: 0px;">We can generally count lysis ranges between dilution 10^-4 and 10^-7.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1032"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1032" data-index="157" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1033"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1033" data-index="158" style="margin-left: 0px;"><b>OD results: </b></div></div></div><div class="mediocre-item" data-scroll-id="note-part1034"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1034-item" data-formattable="true" data-index="159" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Phage titration &amp; infection.xlsx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1034" data-index="159">
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1036"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1036" data-index="160" style="margin-left: 0px;">We can see that the optic density for pBAD-MoClo drops after 60min of infection, like the previous experiment. While OD 600nm for pBAD-T7 &amp; A1 increase for 30 min of infection and then remain stable. Finally, pBAD-B.subtilis OD 600nm remain stable for 30 min of infection and after start to decrease slowly. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1037"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1037" data-index="161" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1038"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1038" data-index="162" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay862"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 20/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1039"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1039" data-index="163" style="margin-left: 35px;"><b>Preculture of colonies for phage infection</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1040"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1040" data-index="164" style="margin-left: 0px;">Incubate Keio Z1F, K12 Hfr in 5mL LB at 37°C. Incubate Keio Z1F' pBAD-MoClo, KeioZ1F' pBAD-A1, KeioZ1F' pBAD-T7, Keio Z1F' pBAD-<i>B. sub </i>in 5mL LB+amp(100ug/ml)+glu(0,2%) at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1041"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1041" data-index="165" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1042"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1042" data-index="166" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay864"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 21/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1043"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1043" data-index="167" style="margin-left: 35px;"><b>Phage infection</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1044"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1044" data-index="168" style="margin-left: 0px;">Dilute each strain (K12 Hfr included) at OD600 = 0,1 in LB +CaCl2(1uL/mL) + MgCl2(1uL/mL) (+amp if necessary), incubate at 37°C.  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1045"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1045" data-index="169" style="margin-left: 0px;">During bacterial growth, prepare soft agar (50% solid agar + 50% liquid agar + <span style="color:#344043">CaCl2(1uL/mL) + MgCl2(1uL/mL)).</span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1046"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1046" data-index="170" style="margin-left: 0px;">Once the indicator strain K12 Hfr reaches an OD=0,4-0,8, add 100µL of K12 Hfr into the soft agar.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1047"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1047" data-index="171" style="margin-left: 0px;">Pour 3mL of soft agar+K12 Hfr on top of LB plates. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1048"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1048" data-index="172" style="margin-left: 0px;">Once the culture reach to OD600=0,4, add arabinose (0,2% final), incubate at <u>37°C for </u>5 minutes.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1049"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1049" data-index="173" style="margin-left: 0px;">Add the volume of phage equals to : V(mL) = (5* volume (mL) of bacteria culture * OD * 5*10^8)/phage concentration.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1050"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1050" data-index="174" style="margin-left: 0px;">Extract immediately 0,5mL of culture for phage titration, this correspond to the timepoint 0, mesure the OD of the culture and reincubate the culture at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1051"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1051" data-index="175" style="margin-left: 0px;">Extract 0,5mL of culture at t=30min, t=60min and t=120min. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1052"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1052" data-index="176" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1053"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1053" data-index="177" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1641" data-scroll-id="note-part1054"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1054-item" data-formattable="true" data-index="178" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="178" data-printable-layout-width="765" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1054-table" data-formattable="true" data-index="178" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 757px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 745px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col><col data-id="5" style="width: 100px;"></col><col data-id="6" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">F</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">F</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">G</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">G</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-0" data-namespace="table-ns1567"><span>Time (min)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-1" data-namespace="table-ns1567"><span>KeioZ1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-2" data-namespace="table-ns1567"><span>KeioZ1 F' pBAD_MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-3" data-namespace="table-ns1567"><span>KEIOZ1 F' pBAD_A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-4" data-namespace="table-ns1567"><span>KeioZ1 F' pBAD_T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-5" data-namespace="table-ns1567"><span>KeioZ1 F' pBAD_B.sub</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-0-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-0" data-namespace="table-ns1567"><span>0</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-1" data-namespace="table-ns1567"><span>0.55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-2" data-namespace="table-ns1567"><span>0.51</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-3" data-namespace="table-ns1567"><span>0.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-4" data-namespace="table-ns1567"><span>0.38</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-1-5" data-namespace="table-ns1567"><span>0.5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-1-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-0" data-namespace="table-ns1567"><span>120</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-1" data-namespace="table-ns1567"><span>1.84</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-2" data-namespace="table-ns1567"><span>1.34</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-3" data-namespace="table-ns1567"><span>0.75</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-4" data-namespace="table-ns1567"><span>0.3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1054-2-5" data-namespace="table-ns1567"><span>0.69</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-2-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-3-0" data-namespace="table-ns1567"><span>Volume of phages to add (MOI = 5)</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-3-1" data-namespace="table-ns1567"><span>394,7</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-3-2" data-namespace="table-ns1567"><span>366,0</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-3-3" data-namespace="table-ns1567"><span>287,1</span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-3-4" data-namespace="table-ns1567"><span>272,7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-3-5" data-namespace="table-ns1567"><span>358,9</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-3-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-4-1" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-3" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-4" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-5" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-4-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-5-1" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-3" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-4" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-5" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-5-6" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignRight cell-contents" data-cid="table-note-part1054-6-1" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-2" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-3" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-4" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-5" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1054-6-6" data-namespace="table-ns1567"><span></span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">OD600 + volume of phage for infection assay</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1054" data-index="178" style="max-width: 765px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1055"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1055" data-index="179" style="margin-left: 0px;">For each timepoint, centrifugate the 0,5mL of culture at max speed for 5min. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1056"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1056" data-index="180" style="margin-left: 0px;">Prepare 10-fold serial dilutions of the supernatant in phage buffer (10^-1 to 10^-9).</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1057"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1057" data-index="181" style="margin-left: 0px;">Add 4µL of chloroform to remaining supernatant, stock phages in chloroform at 4°C.  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1058"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1058" data-index="182" style="margin-left: 0px;">Spot 5uL of the undiluted supernatant and its serial dilutions onto a plate with top agar + K12 Hfr.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1059"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1059" data-index="183" style="margin-left: 0px;">Incubate every plate at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1060"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1060" data-index="184" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1061"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1061" data-index="185" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part1062"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1062-item" data-formattable="true" data-index="186" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Phage titration infection 21082019.xlsx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1062" data-index="186">
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1064"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1064" data-index="187" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1065"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1065" data-index="188" style="margin-left: 35px;"><b>Preculture of colonies for phage infection</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1066"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1066" data-index="189" style="margin-left: 0px;">Incubate K12 Hfr in 5mL LB at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1067"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1067" data-index="190" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay866"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 22/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1068"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1068" data-index="191" style="margin-left: 0px;">Identify the maximum dilutions which permit to get lysis ground. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1069"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1069" data-index="192" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1642" data-scroll-id="note-part1070"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1070-item" data-formattable="true" data-index="193" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="193" data-printable-layout-width="365" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1070-table" data-formattable="true" data-index="193" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 357px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 345px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-0-0" data-namespace="table-ns1567"><span>Replicate</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1070-0-1" data-namespace="table-ns1567"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1070-0-2" data-namespace="table-ns1567"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-1-0" data-namespace="table-ns1567"><span>KeioZ1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-1-1" data-namespace="table-ns1567"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-1-2" data-namespace="table-ns1567"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-2-0" data-namespace="table-ns1567"><span>KeioZ1 F' MoClo</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-2-1" data-namespace="table-ns1567"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-2-2" data-namespace="table-ns1567"><span>10^-5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-3-0" data-namespace="table-ns1567"><span>KeioZ1 F' A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-3-1" data-namespace="table-ns1567"><span>10^-5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-3-2" data-namespace="table-ns1567"><span>10^-5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-4-0" data-namespace="table-ns1567"><span>KeioZ1 F' T7</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-4-1" data-namespace="table-ns1567"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-4-2" data-namespace="table-ns1567"><span>10^-6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-5-0" data-namespace="table-ns1567"><span>KeioZ1 F' B. subtilis</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-5-1" data-namespace="table-ns1567"><span>10^-6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-5-2" data-namespace="table-ns1567"><span>10^-6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-6-0" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-6-1" data-namespace="table-ns1567"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1070-6-2" data-namespace="table-ns1567"><span></span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">t=0 </span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1070" data-index="193" style="max-width: 365px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1071"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1071" data-index="194" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1072"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1072" data-index="195" style="margin-left: 0px;">Prepare soft agar with CaCl2 (1mM final) and MgCl2 (1mM final).</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1073"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1073" data-index="196" style="margin-left: 0px;">Dilute K12 Hfr at a OD600=0,1 in LB, wait until OD600=0,5.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1074"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1074" data-index="197" style="margin-left: 0px;">Add 100uL of K12 Hfr and 100uL of phages in 3mL soft agar. Each phage dilutions were stocked in chloroform (August, 21th). </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1075"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1075" data-index="198" style="margin-left: 0px;">Phages come from the chloroform-conserved-phages .  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1076"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1076" data-index="199" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1077"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1077" data-index="200" style="margin-left: 35px;"><b>Preculture of colonies for phage infection</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1078"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1078" data-index="201" style="margin-left: 0px;">Incubate Keio Z1F, K12 Hfr in 5mL LB at 37°C. Incubate Keio Z1F' pBAD-MoClo, KeioZ1F' pBAD-A1, KeioZ1F' pBAD-T7, Keio Z1F' pBAD-<i>B. sub </i>in 5mL LB+amp(100ug/ml)+glu(0,2%) at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1079"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1079" data-index="202" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1080"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1080" data-index="203" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay868"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 23/8/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1081"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1081" data-index="204" style="margin-left: 35px;"><b>Phage infection : Hugues_Natacha</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1082"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1082" data-index="205" style="margin-left: 0px;">Dilute each strain (K12 Hfr included) at OD600 = 0,1 in LB +CaCl2(1uL/mL) + MgCl2(1uL/mL) (+amp if necessary), incubate at 37°C.  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1083"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1083" data-index="206" style="margin-left: 0px;">During bacterial growth, prepare soft agar (50% solid agar + 50% liquid agar + <span style="color:#344043">CaCl2(1uL/mL) + MgCl2(1uL/mL)).</span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1084"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1084" data-index="207" style="margin-left: 0px;">Once the indicator strain K12 Hfr reaches an OD=0,4-0,8, add 100µL of K12 Hfr into the soft agar.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1085"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1085" data-index="208" style="margin-left: 0px;">Pour 3mL of soft agar+K12 Hfr on top of LB plates. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1086"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1086" data-index="209" style="margin-left: 0px;">Once the culture reach to OD600=0,4, add arabinose (0,2% final), incubate at <u>37°C for 15</u> minutes.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1087"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1087" data-index="210" style="margin-left: 0px;">Add the volume of phage equals to : V(mL) = (5* volume (mL) of bacteria culture * OD * 5*10^8)/phage concentration.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1088"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1088" data-index="211" style="margin-left: 0px;">Extract immediately 0,5mL of culture for phage titration and 1mL of culture for gDNA extraction. This correspond to the timepoint 0, mesure the OD of the culture and reincubate the culture at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1089"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1089" data-index="212" style="margin-left: 0px;">Extract 0,5mL of culture at t=30min, t=90min, t=120min and t=150min. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1090"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1090" data-index="213" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1091"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1091" data-index="214" style="margin-left: 0px;">For each timepoint, centrifugate the 0,5mL of culture at max speed for 5min. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1092"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1092" data-index="215" style="margin-left: 0px;">Prepare 10-fold serial dilutions of the supernatant in phage buffer (10^-1 to 10^-9).</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1093"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1093" data-index="216" style="margin-left: 0px;">Add 4µL of chloroform to remaining supernatant, stock phages in chloroform at 4°C.  </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1094"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1094" data-index="217" style="margin-left: 0px;">Spot 5uL of the supernatant serial dilutions onto a plate with top agar + K12 Hfr.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1095"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1095" data-index="218" style="margin-left: 0px;">Incubate every plate at 37°C. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1096"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1096" data-index="219" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1097"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1097" data-index="220" style="margin-left: 35px;"><b>Genomic DNAs of bacteria infected by phages</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1098"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1098" data-index="221" style="margin-left: 0px;">At different time points (t0, t30, t90, t120, t150) of the phage infection, 1ml of bacterial culture with phage is withdraw. This is done for the four conditions : A1, T7, Moclo and B.sub. The kit used to quantify the genomics DNA of infected bacteria is "QIAamp DNA Blood Mini Kit (50)" from Qiagen. The protocole is "DNA Purification from Tissues" page 32. We started at step 3. Step 1, 2(a, b, c) and 5a are not done. The elution step is done only one time with 200µL of buffer AE. The final solutions are then analyzed with Nanodrops and put on an electrophoresis gel</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1099"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1099" data-index="222" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1100"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1100" data-index="223" style="margin-left: 0px;">Nanodrop results :</div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1643" data-scroll-id="note-part1101"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1101-item" data-formattable="true" data-index="224" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-

Difference between revisions of "Team:GO Paris-Saclay/Survivors"

Latest revision as of 21:09, 19 October 2019

Cheaters