Latest revision as of 21:42, 19 October 2019

<!DOCTYPE html> RNA cell · Benchling

RNA cell

Project: RNA cell: back to the origins

Authors: iGEM_GO_Paris_saclay

Wednesday, 3/7/2019

AIM: As a first step in proving the RNA World concept, we wanted to create an “RNA cell”, in which replicating RNA, rather than DNA, would be the support of genetic information.

Principle: In order to carry out this substitution of DNA, we clone and expresse 3 nucleases: A1, gp3 and yqcg and see if they can efficiently degrade bacterial DNA. In addition to these nucleases, we integrate a plasmid containing The Rna dependent Rna polymerase 5a viral protein which amplifies RNA°. We are going to replicate an anti-sens-GFP-RNA to produce a sens GFP-RNA ,so that RNA can be translated.

●

Cloning Gblocks of T7, B. subtilis and RdRp into pBAD24-MoClo (empty)

The cloning needs a molar ratio insert 2:1 plasmid.

1µL plasmid (PBAD24GG)

+2µL mix (enzymes ligase/XbaI)

+1µL buffer Golden Gate

+ x µL plasmid (for molarity ratio 2:1 cf table1)

Up to 20µL with water

	A A	B B	C C	D D
1 1		Endonuclease T7	Nuclease B. subtilis	RdRp
2 2	Volume (µL ) for Golden Gate cloning	2	7	7

Table1

Heat at 37°C for 15min.

●

Transform pBAD24-Moclo + insert into DH5alpha (see protocol "heat shock plasmid transformation")

2µL pBAD24-Moclo +insert + 50µL competent DH5alpha on ice for 30min

Heatshock at 42°C for 45 seconds

Cool down on ice for 5min then spread on LB+glucose (0.2%) +Amp (100µL/mL)

37°C over night

Thursday, 4/7/2019

●

Results of transformation from July the third [pBAD24-Moclo+insert (endonuclease T7 or nuclease B. subtilis or RdRp)] : Hugues-Natacha-Clara

There are colonies on each Petri dishes (for each cloning) but just a few for the nuclease of B. subtilis.

●

Colony PCR of transformed cells [pBAD24-Moclo+insert (endonuclease T7 or nuclease B. subtilis or RdRp)]:

dNTP diluted 1/2

Primers diluted 1/10 : oligo 5: 557µL

oligo 6: 617 µL

6 colonies for each condition + control: Ligation gblock (endonuclease T7 or nuclease B. subtilis or RdRp) and pBAD 24 GG

	A A	B B	C C
1 1	Primer name	Primer sequence	Tm °C
2 2	2019GO-5-Seq-pBAD-F	5'- TGGCTATGCCATAGCATTTTTATCC	55,6
3 3	2019GO-6-Seq-pBAD-R	5'- GGT CTG ATT TAA TCT GTA TCA GGC TG	55,3

Primer sequences for checking insertion of the inserts

	A A	B B
1 1	Buffer	57,5µL
2 2	dNTP	23µL
3 3	Oligo5	23µL
4 4	Oligo6	23µL
5 5	H2O	195,5µL
6 6	TAQ	3,45µL

PCR master mix

Each colonies was striped on LB + AMP (100µg/mL)+ Glucose 0,2% before using it for the colony PCR and after was sown into liquid culture of LB + AMP + glucose 0.2%

Friday, 5/7/2019

Clara Natacha and Hugues:

●

Stripes of 6 transformed cells with pBAD24-MOCLO, and liquid culture with LB and 100µg/mL of Amp and 0,2% glucose

●

Result of the colony PCR of transformed cells with pBAD MoClo Rdrp and T7

WhatsApp Image 2019-07-05 at 17.27.04.jpeg

All expected signlals have correct sizes even for the golden gate for B. subtilis, so last time we took too much genetic material during the colony PCR

Monday, 8/7/2019

●

gblock of Rdrp, T7 in plasmid pBAD24 MoClo (Clara)

Cultures of DH5alpha cells containing pBAD24 MOCLO clone 1 & 4 and pBAD MoClo Rdrp clone 2' & 5' and pBAD MoClo T7 clone 1' were cultures. Then plasmid DNA extraction was done using NucleoSpin® Plasmid / Plasmid (NoLid) kit. Culture of bacteria containing pBAD24 MOCLO clone 1' to make stock tomorrow are done. Have to do the same for the other transformed cells pBAD MoClo Rdrp clone 2' & 5' and pBAD MoClo T7 clone 1' tomorrow.

Plasmid DNA extraction of pBADMoClo Rdrp clone 1 & 4 and pBADMoClo T7 clone 2 & 1. All DNAs' concentrations were measured with nanodrop.

Sent to sequencing pBADMoClo Rdrp clone 1 & 4 and pBADMoClo T7 clone 2 & 1.

	A A	B B
1 1	plasmid DNA name	Concentration (µg/µL)
2 2	pBADMoClo T7 clone 2	116
3 3	pBADMoClp T7 clone 1	66
4 4	pBADMoClo Rdrp clone 1	89
5 5	pBADMoClo Rdrp clone 4	56
6 6	pBAD24 MOCLO clone 1	42
7 7	pBADMoClo T7 clone 1'	16
8 8	pBADMoClo Rdrp clone 5'	23
9 9	pBADMoClo Rdrp clone 2'	29
10 10

Table3

●

Transformed cells DH5alpha with new golden gate of pBADMoClo nuclease of B. subtilis and the first one. 50 µL of bacteria were used for 1 µL of plasmid. => Tomorrow: Colony PCR and second selection

Tuesday, 9/7/2019

Culture of transformed cells with pBADMoClo Rdrp clone 2' & 5' and pBADMoClo T7 clone 1' for glycerol stock tomorrow.

Thursday, 11/7/2019

gblock in plasmid pBAD24MoClo (Clara)

●

Sequencing result: pBADMoClo T7 clone 1' & RDRP clone 2' great :D

●

Heat shock transformation in Keïo with T7 clone 1' & RDRP clone 2' (see protocol "Heatshock plasmid transformation")

Friday, 12/7/2019

●

Characterization of T7 nuclease and Rdrp (Clara)

	A A	B B	C C	D D	E E
1 1	Time	OD T7 nuclease		OD Rdrp
2 2		Without ARA	With ARA	Without ARA	With ARA
3 3	0 min	0.43	0.43	0.65	0.65
4 4	30 min	0.72	0.21	1.01	1.01
5 5	1 hour	0.98	0.64	1.25	1.31
6 6	2 hour	1.25	0.85	1.68	1.65

Table4

Monday, 15/7/2019

●

Isolation of clones keio T7 and keio Rdrp : Arnaud

Stripes from colonies are done in 2 agar plates with LB + amp + glu

Tuesday, 16/7/2019

●

Bacterial cultures for CFU and OD : Arnaud

keioZ1F'A1

keioZ1F'T7

keioZ1F'Rdrp

keioZ1F'Moclo

in LB + Amp + glu 3 mL

Culture overnight at 37°C

●

Glucose 20% solution : Arnaud

A solution of 500 mL is made with 100 g of Glucose anhydre and is filtrated.

Small solution of 100 mL are made with the big one to reduce contamination

Wednesday, 17/7/2019

Culture of isolated clones (Results) : Abdel_Arnaud

●

60mL culture of A1, T7, Moclo and Rdrp in LB + Amp after a preculture overnight in LB + amp + glucose. Liquide culture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction with Ara when O.D = 0.4. At this moment, each bacterial solution are split in 2 new samples of 25mL. One with arabinose, one whitout.

●

O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (+1110)

●

When arabinose is added, another experiment is done with the bacterial culture. bacterial solutions are used for a perpetual O.D measurement with clariostar.

Results :

A

B

C

D

E

F

G

H

I

J

K

L

1

Time

-30min

+30min

+90min

+150min

+tomorrow

2

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

3

A1

Ara

-3

-1

-3

-1

-4

-1

-6

-2

4

1,10E+01

9,00E+00

2,90E+01

8,00E+00

1,00E+01

3,40E+01

1,40E+01

1,50E+01

5

2,30E+01

1,90E+01

2,90E+01

1,10E+01

1,60E+01

2,50E+01

1,20E+01

2,00E+01

6

1,50E+01

2,00E+01

3,00E+01

1,00E+01

8,00E+00

3,10E+01

1,30E+01

1,90E+01

7

No Ara

-5

-6

-5

-6

8

5,00E+00

6,00E+00

7,00E+00

3,00E+01

4,00E+00

1,40E+01

1,50E+01

2,20E+01

1,80E+01

9

9,00E+00

7,00E+00

1,10E+01

2,50E+01

3,00E+00

1,00E+01

1,30E+01

1,00E+01

1,40E+01

1,80E+01

10

7,00E+00

5,00E+00

2,80E+01

6,00E+00

8,00E+00

1,10E+01

1,20E+01

1,70E+01

11

T7

Ara

-1

-2

-1

-2

-1

-6

-2

12

2,50E+01

5,00E+00

1,00E+01

1,30E+01

2,80E+01

1,50E+01

1,00E+01

7,00E+00

13

2,60E+01

9,00E+00

1,90E+01

1,50E+01

1,70E+01

2,50E+01

1,70E+01

5,00E+00

14

2,10E+01

1,40E+01

1,70E+01

7,00E+00

1,70E+01

2,00E+00

15

No Ara

-4

-5

-4

16

1,50E+01

2,00E+01

1,40E+01

1,20E+01

2,40E+01

1,90E+01

1,00E+01

1,30E+01

1,40E+01

17

1,60E+01

1,70E+01

2,20E+01

2,10E+01

2,30E+01

1,10E+01

9,00E+00

1,90E+01

18

1,90E+01

1,70E+01

2,20E+01

2,60E+01

2,30E+01

4,00E+00

1,10E+01

1,90E+01

19

Moclo

Ara

-5

-6

20

2,70E+01

1,80E+01

9,00E+00

1,00E+01

2,30E+01

21

1,80E+01

2,80E+01

5,00E+00

1,70E+01

2,50E+01

22

1,90E+01

2,20E+01

1,10E+01

1,30E+01

3,00E+01

23

No Ara

-5

-6

24

9,00E+00

1,50E+01

2,80E+01

1,90E+01

8,00E+00

1,50E+01

1,90E+01

25

1,60E+01

1,10E+01

2,80E+01

2,10E+01

6,00E+00

9,00E+00

1,80E+01

26

1,10E+01

1,40E+01

2,20E+01

2,80E+01

5,00E+00

1,40E+01

1,70E+01

2,10E+01

27

Rdrp

Ara

-5

-6

28

2,10E+01

2,40E+01

2,80E+01

1,40E+01

8,00E+00

9,00E+00

5,00E+00

29

2,70E+01

1,40E+01

2,60E+01

2,70E+01

1,30E+01

8,00E+00

7,00E+00

1,00E+01

30

2,50E+01

2,10E+01

2,80E+01

2,40E+01

9,00E+00

6,00E+00

1,20E+01

1,00E+01

31

No Ara

-4

-5

-6

32

1,70E+01

4,00E+00

3,10E+01

2,40E+01

9,00E+00

1,00E+01

1,30E+01

1,50E+01

2,10E+01

1,80E+01

33

1,90E+01

1,30E+01

2,80E+01

2,60E+01

8,00E+00

7,00E+00

1,50E+01

1,20E+01

1,70E+01

2,20E+01

34

2,40E+01

4,00E+00

2,50E+01

2,60E+01

8,00E+00

9,00E+00

1,60E+01

1,10E+01

1,90E+01

2,10E+01

Table5

	A A	B B	C C	D D	E E	F F	G G
1 1	O.D		TIME
2 2			-30	30	90	150	1110
3 3	A1	Ara	0,25	0,60	0,59	0,57	2,90
4 4		No ara	0,25	0,77	1,70	1,88	4,20
5 5	T7	Ara	0,18	0,42	0,37	0,38	2,80
6 6		No ara	0,18	0,62	1,09	1,62	1,00
7 7	Moclo	Ara	0,16	0,70	1,15	1,54	3,90
8 8		No ara	0,16	0,77	1,38	1,85	3,90
9 9	Rdrp	Ara	0,18	0,71	1,20	1,56	5,50
10 10		No ara	0,18	0,74	1,37	1,84	3,90

Table6

Thursday, 18/7/2019

Culture of isolated clones (Results) : Abdel_Arnaud

●

60mL culture of A1, T7, Moclo and Rdrp in LB + Amp after a preculture overnight in LB + amp + glucose. Liquide culture are diluted in order to have an O.D = 0.1 when starting the experiment. Beginning of induction with Ara when O.D = 0.4. At this moment, each bacterial solution are split in 2 new samples of 25mL. One with arabinose, one whitout.

●

O.D and droplet of dilutions in plate are done at different time point compared to the moment arabinose is added (minutes) : -30 ; +30 ; +90 ; +150 ; Tomorow (+1110)

●

When arabinose is added, another experiment is done with the bacterial culture. bacterial solutions are used for a perpetual O.D measurement with clariostar.

Results :

	A A	B B	C C	D D	E E	F F	G G	H H	I I	J J	K K	L L
1 1	Time		-30min		+30min		+90min		+150min		+tomorrow
2 2			Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp
3 3	A1	Ara			-3	-2	-3	-2	-3
4 4					20	9	44	3	32
5 5					21	8		10	38
6 6					25	4		9	34
7 7		No Ara	-4	-4	-6	-6	-6	-6	-5	-5
8 8			16	17	5	5	10	9	29	28
9 9			16	15	2	8	8	7	38	32
10 10			17	18	8	6			40	34
11 11	T7	Ara			-3	-3	-3	-1	-3	-1
12 12					12	12	4	22	14	34
13 13					11	16	5	28	13	37
14 14					10	8		22	11	36
15 15		No Ara	-4	-4	-5	-5	-5	-5	-5	-5
16 16			12	6	19	20	22	20	10	9
17 17

@@ Line 86: / Line 86: @@
 </span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part934-2-0" data-namespace="table-ns1707"><span>2019GO-6-Seq-pBAD-R</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part934-2-1" data-namespace="table-ns1707"><span>5'- GGT CTG ATT TAA TCT GTA TCA GGC TG</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part934-2-2" data-namespace="table-ns1707"><span>55,3</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 35px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Primer sequences for checking insertion of the inserts </span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part934" data-index="34" style="max-width: 765px; margin-left: 35px;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1791" data-scroll-id="note-part935"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part935-item" data-formattable="true" data-index="35" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="35" data-printable-layout-width="265" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part935-table" data-formattable="true" data-index="35" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 35px;"><div class="mediocre-tableEditable-border multi-editable" style="left: 29px; top: -43px; bottom: 4px; width: 257px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 245px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-0-0" data-namespace="table-ns1707"><span>Buffer</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-0-1" data-namespace="table-ns1707"><span>57,5µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-1-0" data-namespace="table-ns1707"><span>dNTP</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-1-1" data-namespace="table-ns1707"><span>23µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-2-0" data-namespace="table-ns1707"><span>Oligo5</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-2-1" data-namespace="table-ns1707"><span>23µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-3-0" data-namespace="table-ns1707"><span>Oligo6</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-3-1" data-namespace="table-ns1707"><span>23µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-4-0" data-namespace="table-ns1707"><span>H2O</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-4-1" data-namespace="table-ns1707"><span>195,5µL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-5-0" data-namespace="table-ns1707"><span>TAQ</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part935-5-1" data-namespace="table-ns1707"><span>3,45µL</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 35px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">PCR master mix</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part935" data-index="35" style="max-width: 265px; margin-left: 35px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part936"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="36" style="margin-left: 35px;">Each colonies was striped on LB + AMP <span style="color:#344043"><span style="background-color:#FFFFFF">(100µg/mL)</span></span>+ Glucose 0,2% before using it for the colony PCR and after was sown into liquid culture of LB + AMP + glucose 0.2%</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part937"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part937" data-index="37" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part938"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="38" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part939"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="39" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay846"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part940"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part940" data-index="40" style="margin-left: 0px;"><b><u>Clara Natacha and Hugues</u></b>:</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part941"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part941" data-index="41" style="margin-left: 35px;">Stripes of 6 transformed cells with pBAD24-MOCLO, and liquid culture with LB and 100µg/mL of Amp and 0,2% glucose </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part942"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="42" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">Result of the c</span></span>olony PCR of transformed cells with pBAD MoClo Rdrp and T7 </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part943"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part943" data-index="43" style="margin-left: 0px;"> </div></div></div><div class="mediocre-item" data-scroll-id="note-part944"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part944-item" data-formattable="true" data-index="44" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">WhatsApp Image 2019-07-05 at 17.27.04.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/trdUpDLMWyzBYkH9ngUuhWrgWA2wxD6Ikr6Tqnde-WhatsApp_Image_2019-07-05_at_17.27.04.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part944" data-index="44">All expected signlals have correct sizes even for the golden gate for <i>B. subtilis</i>, so last time we took too much genetic material during the colony PCR
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part936"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="36" style="margin-left: 35px;">Each colonies was striped on LB + AMP <span style="color:#344043"><span style="background-color:#FFFFFF">(100µg/mL)</span></span>+ Glucose 0,2% before using it for the colony PCR and after was sown into liquid culture of LB + AMP + glucose 0.2%</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part937"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part937" data-index="37" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part938"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="38" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part939"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="39" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay846"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part940"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part940" data-index="40" style="margin-left: 0px;"><b><u>Clara Natacha and Hugues</u></b>:</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part941"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part941" data-index="41" style="margin-left: 35px;">Stripes of 6 transformed cells with pBAD24-MOCLO, and liquid culture with LB and 100µg/mL of Amp and 0,2% glucose </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part942"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="42" style="margin-left: 35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">Result of the c</span></span>olony PCR of transformed cells with pBAD MoClo Rdrp and T7 </div>
+<figure class="mbr-figure container" style="width: 25%;margin:auto">
+        <div class="image-block" style="width: 25%;margin:auto">
+            <img src="https://2019.igem.org/wiki/images/a/a1/T--Go_Paris-Saclay--Axxgsdtezxxx1.png"  alt="NuclA1" title="">
+        </div>
+    </figure>
+</div></div><div class="mediocre-item is-text" data-scroll-id="note-part943"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part943" data-index="43" style="margin-left: 0px;"> </div></div></div><div class="mediocre-item" data-scroll-id="note-part944"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part944-item" data-formattable="true" data-index="44" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">WhatsApp Image 2019-07-05 at 17.27.04.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/trdUpDLMWyzBYkH9ngUuhWrgWA2wxD6Ikr6Tqnde-WhatsApp_Image_2019-07-05_at_17.27.04.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part944" data-index="44">All expected signlals have correct sizes even for the golden gate for <i>B. subtilis</i>, so last time we took too much genetic material during the colony PCR
 </div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part946"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="45" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part947"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="46" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part948"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="47" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay848"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part949"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part949" data-index="48" style="margin-left: 35px;"><b>gblock of Rdrp, T7 in plasmid pBAD24 MoClo (Clara)</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part950"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part950" data-index="49" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part951"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="50" style="margin-left: 0px;">Cultures of DH5alpha cells containing pBAD24 MOCLO clone 1 &amp; 4 and pBAD MoClo Rdrp clone 2' &amp; 5' and <span style="color:#344043"><span style="background-color:#FFFFFF">pBAD MoClo T7 clone 1' were cultures. Then plasmid DNA extraction was done using </span></span><i><span style="color:#344043"><span style="background-color:#FFFFFF">NucleoSpin® Plasmid / Plasmid (NoLid)</span></span></i><span style="color:#344043"><span style="background-color:#FFFFFF"> kit. Culture of bacteria containing </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBAD24 MOCLO clone 1' <b><u>to make stock tomorrow </u></b>are done.  Have to do the same for the other transformed cells </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBAD MoClo Rdrp clone 2' &amp; 5' and </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBAD MoClo T7 clone 1' <b><u>tomorrow</u></b>. </span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part952"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="51" style="margin-left: 0px;">Plasmid DNA extraction of <span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo Rdrp clone 1 &amp; 4 and </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo T7 clone 2 &amp; 1. All DNAs' concentrations were measured with <i>nanodrop</i>.</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part953"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part953" data-index="52" style="margin-left: 0px;">Sent to sequencing <span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo Rdrp clone 1 &amp; 4 and </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo T7 clone 2 &amp; 1</span></span>. </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part954"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="53" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part955"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part955" data-index="54" style="margin-left: 35px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1792" data-scroll-id="note-part956"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part956-item" data-formattable="true" data-index="55" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="55" data-printable-layout-width="265" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part956-table" data-formattable="true" data-index="55" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 35px;"><div class="mediocre-tableEditable-border multi-editable" style="left: 29px; top: -43px; bottom: 4px; width: 257px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 245px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-0-0" data-namespace="table-ns1707"><span>plasmid DNA name</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-0-1" data-namespace="table-ns1707"><span>Concentration (µg/µL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-1-0" data-namespace="table-ns1707"><span>pBADMoClo T7 clone 2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-1-1" data-namespace="table-ns1707"><span>116</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-2-0" data-namespace="table-ns1707"><span>pBADMoClp T7 clone 1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-2-1" data-namespace="table-ns1707"><span>66</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-3-0" data-namespace="table-ns1707"><span>pBADMoClo Rdrp clone 1 </span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-3-1" data-namespace="table-ns1707"><span>89</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-4-0" data-namespace="table-ns1707"><span>pBADMoClo Rdrp clone 4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-4-1" data-namespace="table-ns1707"><span>56</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-5-0" data-namespace="table-ns1707"><span> pBAD24 MOCLO clone 1 </span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-5-1" data-namespace="table-ns1707"><span>42</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-6-0" data-namespace="table-ns1707"><span>pBADMoClo T7 clone 1'</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-6-1" data-namespace="table-ns1707"><span>16</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-7-0" data-namespace="table-ns1707"><span>pBADMoClo Rdrp clone 5'</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-7-1" data-namespace="table-ns1707"><span>23</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">9</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">9</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-8-0" data-namespace="table-ns1707"><span>pBADMoClo Rdrp clone 2'</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part956-8-1" data-namespace="table-ns1707"><span>29</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">10</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">10</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-9-0" data-namespace="table-ns1707"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part956-9-1" data-namespace="table-ns1707"><span></span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 35px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table3</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part956" data-index="55" style="max-width: 265px; margin-left: 35px;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part957"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="56" style="margin-left: 35px;">Transformed cells DH5alpha with new golden gate of pBADMoClo nuclease of <i>B. subtilis</i> and the first one. 50 µL of bacteria were used for 1 µL of plasmid.  =&gt; Tomorrow: Colony PCR and second selection </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part958"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part958" data-index="57" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part959"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part959" data-index="58" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay850"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part960"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="59" style="margin-left: 0px;">Culture of <span style="color:#344043"><span style="background-color:#FFFFFF">transformed cells with </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo Rdrp clone 2' &amp; 5' and </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">pBADMoClo T7 clone 1'</span></span> for glycerol stock <b>tomorrow</b>.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part961"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part961" data-index="60" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part962"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part962" data-index="61" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay852"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 11/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part963"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="62" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part964"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part964" data-index="63" style="margin-left: 0px;"><b><span style="color:#344043"><span style="background-color:#FFFFFF">gblock in plasmid pBAD24MoClo (Clara)</span></span></b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part965"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part965" data-index="64" style="margin-left: 35px;">Sequencing result: pBADMoClo T7 clone 1' &amp; RDRP clone 2' great :D</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part966"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="65" style="margin-left: 35px;">Heat shock transformation in Keïo with <span style="color:#344043"><span style="background-color:#FFFFFF">T7 clone 1' &amp; RDRP clone 2' (see protocol "Heatshock plasmid transformation")</span></span></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part967"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="66" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part968"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="67" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay854"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 12/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part969"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="68" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part970"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="69" style="margin-left: 35px;"><b>Characterization of T7 nuclease and Rdrp (Clara) </b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part971"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="70" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print1793" data-scroll-id="note-part972"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part972-item" data-formattable="true" data-index="71" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="71" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part972-table" data-formattable="true" data-index="71" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 70px;"><div class="mediocre-tableEditable-border multi-editable" style="left: 64px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-0-0" data-namespace="table-ns1707"><span>Time</span></td><td class="cell is-text is-alignCenter cell-contents" colspan="2" data-cid="table-note-part972-0-1" data-namespace="table-ns1707" rowspan="1"><span>OD T7 nuclease</span></td><td class="cell is-text is-alignCenter cell-contents" colspan="2" data-cid="table-note-part972-0-3" data-namespace="table-ns1707" rowspan="1"><span>OD Rdrp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-1-0" data-namespace="table-ns1707"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-1-1" data-namespace="table-ns1707"><span>Without ARA</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-1-2" data-namespace="table-ns1707"><span>With ARA</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-1-3" data-namespace="table-ns1707"><span>Without ARA</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-1-4" data-namespace="table-ns1707"><span>With ARA</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-2-0" data-namespace="table-ns1707"><span>0 min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-2-1" data-namespace="table-ns1707"><span>0.43</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-2-2" data-namespace="table-ns1707"><span>0.43</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-2-3" data-namespace="table-ns1707"><span>0.65</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-2-4" data-namespace="table-ns1707"><span>0.65</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-3-0" data-namespace="table-ns1707"><span>30 min</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-3-1" data-namespace="table-ns1707"><span>0.72</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-3-2" data-namespace="table-ns1707"><span>0.21</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-3-3" data-namespace="table-ns1707"><span>1.01</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-3-4" data-namespace="table-ns1707"><span>1.01</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-4-0" data-namespace="table-ns1707"><span>1 hour</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-4-1" data-namespace="table-ns1707"><span>0.98</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-4-2" data-namespace="table-ns1707"><span>0.64</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-4-3" data-namespace="table-ns1707"><span>1.25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-4-4" data-namespace="table-ns1707"><span>1.31</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part972-5-0" data-namespace="table-ns1707"><span>2 hour</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-5-1" data-namespace="table-ns1707"><span>1.25</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-5-2" data-namespace="table-ns1707"><span>0.85</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-5-3" data-namespace="table-ns1707"><span>1.68</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part972-5-4" data-namespace="table-ns1707"><span>1.65</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 70px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Table4</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part972" data-index="71" style="max-width: 565px; margin-left: 70px;">

Difference between revisions of "Team:GO Paris-Saclay/RNACellOrigins"

Latest revision as of 21:42, 19 October 2019

RNA cell

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