Revision as of 20:22, 20 October 2019

<!DOCTYPE html> Nuclease_A1 (from bacteriophage T5) · Benchling

Nuclease_A1 (from bacteriophage T5)

Project: Nucleases characterizations

Authors: iGEM_GO_Paris_saclay

Monday, 1/7/2019

Aim: Insert the gene A1 in pBD24MoClo with golden gate method in order to express A1 nuclease under the control of the inducive promoter arabinose-dependent promoter P_ara.

Heat shock transformation of competent cells Escherichia coli KeioZ1 F’ with pBAD24GG and pBAD24A1 : Brahim _ Arnaud _ Laura

See the protocol "heat shock transformation".

The transformants were selected on LB agar plates plus Ampicilline (100µg/mL) and 0.2% glucose.

Tuesday, 2/7/2019

RESULTS of transformation of KeioZ1 F' cells

A

B

C

D

E

F

G

H

I

J

K

L

M

1

pBAD24A1

pBAD24GG

Without Plasmid

2

Amp / Without Amp

Yes

No

Yes

No

Yes

No

3

Volume spread (µL)

50

100

100 concentrated

100

50

100

100 concentrated

100

50

100

100 concentrated

100

4

Results

Colonies

No colonies

Colonies

Table3

Controls are OK. Isolated colonies are available to continue our experiment.

Isolation of single colony : Laura_Arnaud_Brahim

Single colonies are picked and spread onto a new agar plate with LB+Amp+Glucose in an attempt to isolate single colonies.

Agar plates are then incubated at 37°C overnight.

Wednesday, 3/7/2019

Culture of isolated clones : Laura_Arnaud_Brahim

Isolated clones are put in liquid LB+Amp (100µg/µL) + 0.2% Glucose overnight at 37°C.

Friday, 5/7/2019

Culture of isolated clones (Results) : Laura_Arnaud_Brahim_Marie

Results :

	A A	B B	C C	D D	E E
1 1		GG (futur Ara)	GG	A1 (futur Ara)	A1
2 2	DO	0,58	0,62	0,63	0,60

Table1

Beginning of induction with Ara and first measurement at 30min then every hour.

Spread on petri dishes from transformed cells

	A A	B B	C C	D D	E E	F F	G G	H H	I I	J J	K K	L L	M M	N N	O O	P P	Q Q
1 1	A1	-30 min			+30 min			1h30 min			2H 30			Demain
2 2		Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	LB Glu	LB Amp	LB Amp Glu
3 3	ara	Tapis			4	2	4	12	8	8	7	1	5	6	8	6
4 4					4	3	3	11	13	8	2	1	2	6	12	8
5 5					2	3	7	14	12	12	1	3	2	5	8	4
6 6	Non ara	Tapis			2	4	2	T	13	10	5	8	6	0	0	0
7 7					3	2	5	T	-	6	2	4	4	0	0	0
8 8					3	9	3	T	-	6	5	6	-	0	0	0
9 9
10 10

CFU in 10^8 (cell/mL)

	A A	B B	C C	D D	E E	F F	G G	H H	I I	J J	K K	L L	M M	N N	O O	P P
1 1	GG	-30 min			+30 min			1h30 min			2H 30			Demain
2 2		Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	Amp Glu	Amp	Glu	LB Amp Glu	LB Amp	LB Glu
3 3	ara	Tapis			5	8	19	9	17	15	1	2	2	0	0	3
4 4					3	4	20	15	16	12	2	3	1	1	3	1
5 5					5	4	20	15	11	17	2	3	4	1	1	0
6 6	Non ara	Tapis			5	3	6	20	37	30	1	0	0	5	3	5
7 7					4	4	3	T	34	T	0	1	1	6	5	5
8 8					4	3	1	15	37	T	0	0	0	4	3	6

CFU in 10^8 ( cell/mL)

Second step: reading the OD using the plate spectrophotometer.

Measurement automatically of the DO every 20min during 12hours.

	A A	B B	C C	D D	E E
1 1		1	2	3	4
2 2	A	A1 Ara	A1	GG Ara	GG
3 3	B	A1 Ara	A1	GG Ara	GG
4 4	C	A1 Ara	A1	GG Ara	GG

Plate Plan

Results :

i

Monday, 8/7/2019

1.

Characterization of the A1 nuclease : Marie - Laura

Transformation verification : colony PCR. For each sample : pBAD24A1.1, pBAD24A1.2, pBAD24GG.1, pBAD24GG.2 transformed in E.coli on 1st/2nd july :

	A A	B B	C C
1 1	Primer name	Primer sequence	Tm °C
2 2	2019GO-5-Seq-pBAD-F	5'- TGGCTATGCCATAGCATTTTTATCC	55,6
3 3	2019GO-6-Seq-pBAD-R	5'- GGT CTG ATT TAA TCT GTA TCA GGC TG	55,3

Primer sequences for checking insertion of the inserts

	A A	B B
1 1	2,5 μL	Green taq Buffer
2 2	1 μL	dNTP
3 3	1 μL	Amorce5
4 4	1 μL	Amorce6
5 5	10 μL	Colony ADN + 10 μL of water
6 6	0,15 μL	Taq polymerase
7 7	9,5 μL	H2O
8 8	Vtot = 25 μL

Table2

PCR program (named "IGM") :

94°C/5'

30 cycles : 94°C/30''

51°C/30''

72°C/2min

72°C/10'

Electrophoresis : 100V, 10 μL sample/well, 25 minutes.

Results :

Gel IGEm 09.07.19.001.jpeg

Pre-culture : From glycerol stock on 1st/2nd july (pBAD24A1.1, pBAD24A1.2, pBAD24GG.1, pBAD24GG.2 transformed in E.coli), for each sample : 2 mL LB + 100μg/mL Ampicilline + 20% glucose, incubation with agitation overnight.

Tuesday, 9/7/2019

Culture of isolated clones (Results) : Laura_Arnaud_Marie

60mL culture of A1 and GG. Then beginning of induction with Ara in 30mL of A1 and GG, and first measurement of Optic Density at 30min each sample, then every hour twice.

Results :

	A A	B B	C C	D D	E E	F F	G G
1 1		A1 (60mL)	GG (60mL)	A1+Ara (30mL)	A1 (30mL)	GG+Ara (30mL)	GG (30mL)
2 2	t = -45min	0,12	0,13
3 3	t = -15min	0,22	0,23
4 4	t = t0 induction	0,33	0,38
5 5	t = +30min			0,65	0,74	0,80	0,81
6 6	t = +1h30			0,66	1,39	1,31	1,48
7 7	t = +2h30			0,63	1,89	1,68	1,97
8 8	t = +18h30			1,9	3,8	3,1	3,8

Table4

Rq : We can notice that the population of A1+Ara bacteria decreases over time until 2h30. Then, during the night bacteria develop. We can assume that bacteria mutate during the night and grow.

Box spreading from transformers' solutions.

	A A	B B	C C	D D	E E	F F	G G	H H	I I	J J	K K	L L
1 1	Time		-30min		+30min		+1h30		+2h30		+tomorrow
2 2			Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp	Glu	Glu+Amp
3 3	A1	Ara				10—2	10—3	10—2	10—4	10—6	10—6	10—2
4 4						11	10	2	5	1	7
5 5						10	8	2	4	1	9	21
6 6						10	15	2	9	1	11	16
7 7		No Ara	10—4	10—4	10—5	10—5		10—6	10—6	10—6	10—6	10—6
8 8			22	19	12	14		7	10—6	16	5	11
9 9			18	24	11	8		6	9	21	7	10
10 10			21	27	15	17		9	9	17	7	14
11 11	GG	Ara			10—5	10—5	10—5	10—6	10—6	10—6	10—6	10—6
12 12					16	20	35	9	16	10	13	8
13 13					6	12	27	11	14	6	9	6
14 14					6	5	34	8	14	14	10	16
15 15		No Ara	10—4	10—4	10—5	10—5	10—6	10—5	10—6	10—6	10—6	10—6
16 16			15	33	15	16	13	25	10	16	10	12
17 17			12	26	12	18	7	34	11	15	8	13
18 18			10		17	20	12	33		19		12
19 19			10		17	20	12	33		9		12

Results

A

B

C

D

E

F

G

H

I

J

K

L

1

Time

-30min

+30min

+1h30

+2h30

+tomorrow

2

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

Glu

Glu+Amp

3

A1

Ara

1,03E+05

1,10E+06

2,00E+04

6,00E+06

1,00E+06

9,00E+08

1,23E+09

4

5,77E+03

3,61E+05

0,00E+00

2,65E+06

0,00E+00

2,00E+08

1,10E+09

5

6

7

No Ara

2,03E+07

2,33E+07

1,27E+07

1,30E+07

7,33E+08

9,00E+08

1,80E+09

6,33E+08

1,17E+09

8

2,08E+06

4,04E+06

2,08E+06

4,58E+06

1,53E+08

0,00E+00

2,65E+08

1,15E+08

2,08E+08

9

10

11

GG

Ara

9,33E+07

1,23E+08

3,20E+08

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class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td 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class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">J</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">J</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">K</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">K</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">L</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">L</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">M</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">M</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">N</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">N</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">O</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">O</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">P</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">P</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-0-0" data-namespace="table-ns2427"><span>GG</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-0-1" data-namespace="table-ns2427"><span>-30 min</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-0-4" data-namespace="table-ns2427"><span>+30 min</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-0-7" data-namespace="table-ns2427"><span>1h30 min</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-0-10" data-namespace="table-ns2427"><span>2H 30</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-0-13" data-namespace="table-ns2427"><span>Demain</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-0" data-namespace="table-ns2427"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-1" data-namespace="table-ns2427"><span>Amp   Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-2" data-namespace="table-ns2427"><span>Amp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-3" data-namespace="table-ns2427"><span>Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-4" data-namespace="table-ns2427"><span>Amp   Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-5" data-namespace="table-ns2427"><span>Amp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-6" data-namespace="table-ns2427"><span>Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-7" data-namespace="table-ns2427"><span>Amp   Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-8" data-namespace="table-ns2427"><span>Amp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-9" data-namespace="table-ns2427"><span>Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-10" data-namespace="table-ns2427"><span>Amp   Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-11" data-namespace="table-ns2427"><span>Amp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-12" data-namespace="table-ns2427"><span>Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-13" data-namespace="table-ns2427"><span>LB Amp  Glu</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-14" data-namespace="table-ns2427"><span>LB Amp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-1-15" data-namespace="table-ns2427"><span>LB Glu</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-2-0" data-namespace="table-ns2427" rowspan="3"><span>ara</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-2-1" data-namespace="table-ns2427" rowspan="3"><span>Tapis</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-4" data-namespace="table-ns2427"><span>5</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-5" data-namespace="table-ns2427"><span>8</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-6" data-namespace="table-ns2427"><span>19</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-7" data-namespace="table-ns2427"><span>9</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-8" data-namespace="table-ns2427"><span>17</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-9" data-namespace="table-ns2427"><span>15</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-10" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-11" data-namespace="table-ns2427"><span>2</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-12" data-namespace="table-ns2427"><span>2</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-13" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-14" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-2-15" data-namespace="table-ns2427"><span>3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-4" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-5" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-6" data-namespace="table-ns2427"><span>20</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-7" data-namespace="table-ns2427"><span>15</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-8" data-namespace="table-ns2427"><span>16</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-9" data-namespace="table-ns2427"><span>12</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-10" data-namespace="table-ns2427"><span>2</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-11" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-12" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-13" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-14" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-3-15" data-namespace="table-ns2427"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">5</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">5</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-4" data-namespace="table-ns2427"><span>5</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-5" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-6" data-namespace="table-ns2427"><span>20</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-7" data-namespace="table-ns2427"><span>15</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-8" data-namespace="table-ns2427"><span>11</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-9" data-namespace="table-ns2427"><span>17</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-10" data-namespace="table-ns2427"><span>2</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-11" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-12" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-13" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-14" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-4-15" data-namespace="table-ns2427"><span>0</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">6</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">6</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-5-0" data-namespace="table-ns2427" rowspan="3"><span>Non ara</span></td><td class="cell is-text is-alignLeft cell-contents" colspan="3" data-cid="table-note-part1238-5-1" data-namespace="table-ns2427" rowspan="3"><span>Tapis</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-4" data-namespace="table-ns2427"><span>5</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-5" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-6" data-namespace="table-ns2427"><span>6</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-7" data-namespace="table-ns2427"><span>20</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-8" data-namespace="table-ns2427"><span>37</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-9" data-namespace="table-ns2427"><span>30</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-10" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-11" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-12" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-13" data-namespace="table-ns2427"><span>5</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-14" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-5-15" data-namespace="table-ns2427"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">7</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">7</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-4" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-5" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-6" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-6-7" data-namespace="table-ns2427"><span>T</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-8" data-namespace="table-ns2427"><span>34</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-6-9" data-namespace="table-ns2427"><span>T</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-10" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-11" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-12" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-13" data-namespace="table-ns2427"><span>6</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-14" data-namespace="table-ns2427"><span>5</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-6-15" data-namespace="table-ns2427"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">8</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">8</span><div class="u-flex-display"></div></div></div></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-4" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-5" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-6" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-7" data-namespace="table-ns2427"><span>15</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-8" data-namespace="table-ns2427"><span>37</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1238-7-9" data-namespace="table-ns2427"><span>T</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-10" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-11" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-12" data-namespace="table-ns2427"><span>0</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-13" data-namespace="table-ns2427"><span>4</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-14" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignLeft cell-contents" data-cid="table-note-part1238-7-15" data-namespace="table-ns2427"><span>6</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">CFU in 10^8 ( cell/mL)</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1238" data-index="25" style="max-width: 1670px; margin-left: 0px;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1239"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1239" data-index="26" style="margin-left: 0px;">Second step: reading the OD using the plate spectrophotometer.</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1240"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1240" data-index="27" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1241"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1241" data-index="28" style="margin-left: 0px;">Measurement automatically of the DO every 20min during 12hours.</div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print2574" data-scroll-id="note-part1242"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1242-item" data-formattable="true" data-index="29" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="29" data-printable-layout-width="565" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1242-table" data-formattable="true" data-index="29" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 557px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 545px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 100px;"></col><col data-id="1" style="width: 100px;"></col><col data-id="2" style="width: 100px;"></col><col data-id="3" style="width: 100px;"></col><col data-id="4" style="width: 100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">D</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">D</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">E</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">E</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-0-0" data-namespace="table-ns2427"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1242-0-1" data-namespace="table-ns2427"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1242-0-2" data-namespace="table-ns2427"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1242-0-3" data-namespace="table-ns2427"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1242-0-4" data-namespace="table-ns2427"><span>4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-1-0" data-namespace="table-ns2427"><span>A</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-1-1" data-namespace="table-ns2427"><span>A1 Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-1-2" data-namespace="table-ns2427"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-1-3" data-namespace="table-ns2427"><span>GG Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-1-4" data-namespace="table-ns2427"><span>GG</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-2-0" data-namespace="table-ns2427"><span>B</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-2-1" data-namespace="table-ns2427"><span>A1 Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-2-2" data-namespace="table-ns2427"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-2-3" data-namespace="table-ns2427"><span>GG Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-2-4" data-namespace="table-ns2427"><span>GG</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">4</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">4</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-3-0" data-namespace="table-ns2427"><span>C</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-3-1" data-namespace="table-ns2427"><span>A1 Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-3-2" data-namespace="table-ns2427"><span>A1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-3-3" data-namespace="table-ns2427"><span>GG Ara</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1242-3-4" data-namespace="table-ns2427"><span>GG</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Plate Plan</span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1242" data-index="29" style="max-width: 565px; margin-left: 0px;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1243"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1243" data-index="30" style="margin-left: 0px;">Results : </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1244"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1244" data-index="31" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part1245"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1245-item" data-formattable="true" data-index="32" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/3fWfwle3A50zvUf3m3gPLbI7l0q4d4rASH2Wc6lX-image.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1245" data-index="32">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1243"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1243" data-index="30" style="margin-left: 0px;">Results : </div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1244"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1244" data-index="31" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part1245"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1245-item" data-formattable="true" data-index="32" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">
-</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1247"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1247" data-index="33" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part1248"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1248-item" data-formattable="true" data-index="34" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/4uPCC4mfDE2h7Og18b6mBMAPePTaCqcoj5RiGeIn-image.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1248" data-index="34">
+<img src="https://2019.igem.org/wiki/images/c/cf/T--Go_Paris-Saclay--Ascadaace1.png">
+</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/3fWfwle3A50zvUf3m3gPLbI7l0q4d4rASH2Wc6lX-image.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1245" data-index="32">
+</div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1247"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1247" data-index="33" style="margin-left: 0px;"></div></div></div><div class="mediocre-item" data-scroll-id="note-part1248"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1248-item" data-formattable="true" data-index="34" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="mediocre-captionEditable-indent" style="padding-left: 0px;"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"></div><div class="pull-right"><span></span></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">i</div>
+<img src="https://2019.igem.org/wiki/images/c/cf/T--Go_Paris-Saclay--Ascadaace1.png">
+</h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="assets/4uPCC4mfDE2h7Og18b6mBMAPePTaCqcoj5RiGeIn-image.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width: 0px;"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1248" data-index="34">
 </div></div></div></div></div></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1250"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1250" data-index="35" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1251"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1251" data-index="36" style="margin-left: 0px;"></div></div></div><div class="daySeparator" data-scroll-id="entryDay1173"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/7/2019</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1252"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1252" data-index="37" style="margin-left: 35px;"><b>Characterization of the A1 nuclease : Marie - Laura</b></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1253"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1253" data-index="38" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1254"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="39" style="margin-left: 0px;">Transformation verification : colony PCR. For each sample : pBAD24A1.1, pBAD24A1.2, pBAD24GG.1, pBAD24GG.2 transformed in E.coli on 1st/2nd july :</div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1255"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="40" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-text" data-scroll-id="note-part1256"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="41" style="margin-left: 0px;"></div></div></div><div class="mediocre-item is-captionedTable" data-scale-for-print="print2575" data-scroll-id="note-part1257"><div class=""><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1257-item" data-formattable="true" data-index="42" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table"><div class="mediocre-captionEditable-indent"><div class="mediocre-captionEditable-grid"><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width: 100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="42" data-printable-layout-width="765" data-subindex="cell"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable hiddenFocusEditable--fullTable" contenteditable="true" data-cid="note-part1257-table" data-formattable="true" data-index="42" data-subindex="table"></div><div class="u-position-relative mediocre-tableEditable-scrollAreaWrapper" style="margin-left: 0px; margin-right: 0px;"><div class="mediocre-tableEditable-scrollArea" style="padding-top: 47px;"><div class="mediocre-tableEditable-tableWrapper" style="padding-left: 0px;"><div class="mediocre-tableEditable-border multi-editable" style="left: -6px; top: -43px; bottom: 4px; width: 757px; min-width: 12px;"></div><div class="u-position-relative u-flex-display"><table class="mediocre-tableEditable-table mediocre-tableEditable-table--main" style="width: 745px;"><colgroup><col style="width: 45px;"></col><col data-id="0" style="width: 207px;"></col><col data-id="1" style="width: 367px;"></col><col data-id="2" style="width: 126px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--header"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">A</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">A</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">B</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">B</span><div class="u-flex-display"></div></div></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible mediocre-tableEditable-axisCell--header">C</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text mediocre-tableEditable-axisCell--header">C</span><div class="u-flex-display"></div></div></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">1</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">1</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-0-0" data-namespace="table-ns2427"><span>Primer name</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-0-1" data-namespace="table-ns2427"><span>Primer sequence</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-0-2" data-namespace="table-ns2427"><span>Tm °C</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">2</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">2</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-1-0" data-namespace="table-ns2427"><span>2019GO-5-Seq-pBAD-F</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-1-1" data-namespace="table-ns2427"><span>5'- TGGCTATGCCATAGCATTTTTATCC</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-1-2" data-namespace="table-ns2427"><span>55,6
 </span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-labelWrapper"><div class="mediocre-tableEditable-axisCell-labelSizer u-overflow-wrap-break-word invisible">3</div><div class="u-flex-display invisible"></div><div class="mediocre-tableEditable-axisCell-label u-verticalCenterItems"><span class="mediocre-tableEditable-axisCell-label-text u-ellipsis-text">3</span><div class="u-flex-display"></div></div></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-2-0" data-namespace="table-ns2427"><span>2019GO-6-Seq-pBAD-R</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-2-1" data-namespace="table-ns2427"><span>5'- GGT CTG ATT TAA TCT GTA TCA GGC TG</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1257-2-2" data-namespace="table-ns2427"><span>55,3</span></td></tr></tbody><tfoot></tfoot></table><div class="mediocre-tableEditable-fillerTableWrapper" style="margin-right: 10px;"><table class="mediocre-tableEditable-fillerTable" style="display: none;"><tbody><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr><tr style="height: 0px;"><td></td></tr></tbody></table></div></div></div></div></div><div class="mediocre-tableEditable-titleBarWrapper"><div class="mediocre-tableEditable-titleBar" style="height: 37px; padding-left: 0px;"><div class="mediocre-tableEditable-tableName u-pointer"><div class="u-flex-grow u-overflow-hidden u-verticalCenterItems"><div class="u-ellipsis-text"><span class="u-ellipsis-text">Primer sequences for checking insertion of the inserts </span></div></div><div class="u-flex-none"></div></div><div class="u-flex-none u-tiny-spacing-left u-verticalCenterItems table-floater"><svg class="svgIcon workflowTable-tableCollapser u-pointer"><use xlink:href="#chevron-up_c0f0f77c91b0d9072b0d8255f9f54e3c"></use></svg></div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="mediocre-captionEditable-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1257" data-index="42" style="max-width: 765px; margin-left: 0px;">

Difference between revisions of "Team:GO Paris-Saclay/NucleasesCharacterization/A1"

Revision as of 20:22, 20 October 2019

Nuclease_A1 (from bacteriophage T5)

i

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