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Difference between revisions of "Team:CAU China/Results"

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−
 
 
{{CAU_China/CSS/Top}}
 
{{CAU_China/CSS/Top}}
 
<html>
 
<html>
−
<div class="showCase container-fluid jumbotron jumbotron-fluid text-center bg-white align-items-center text-light mb-0">
+
<style>
−
         <div class="row" style="">
+
 
−
            <div class="col-12 col-md-6">
+
.pictureTitle{
−
               
+
    background: linear-gradient(rgba(0,0,0,.3), rgba(0,0,0,.5)), url("");
−
            </div>
+
    background-repeat: no-repeat;
−
            <div class="col-12 col-md-6 p-5">
+
    background-size: cover;
−
               
+
    background-position:center;
−
                <span class="d-block mt-5">
+
}
−
               
+
 
−
             
+
#accordion .card {
−
               
+
    margin-bottom: 10px;
−
            </div>
+
    border: 2px solid rgba(0,0,0,.4);
 +
}
 +
#accordion .card-header {
 +
padding:0;
 +
background-color:#00A7D4;
 +
}
 +
#accordion h5 {
 +
padding:0;
 +
}
 +
#accordion .card button{
 +
width: 100%;
 +
text-align: left!important;
 +
}
 +
#accordion button span {
 +
    font-size: 1.5rem;
 +
}
 +
 
 +
@media only screen and (max-width: 809.99px) {
 +
    #accordion button span {
 +
    font-size: 1rem;
 +
    }
 +
}
 +
 
 +
@media only screen and (max-width: 575.98px) {}
 +
 
 +
@media only screen and (max-width: 767.98px) {}
 +
 
 +
@media only screen and (max-width: 991.98px) {}
 +
 
 +
@media only screen and (max-width: 1199.98px) {}
 +
</style>
 +
 
 +
<div class="pictureTitle container-fluid text-center mb-0 align-items-center text-light">
 +
 
 +
                    <div class="display-2 mb-0">
 +
                    Results
 +
                    </div>
 +
         <h4></h4>
 +
 
 +
</div>
 +
 
 +
 
 +
<div class="phaContainer">
 +
  <aside id="phaContentsOuter">
 +
  <aside id="phaContents" class="table-of-contents">
 +
    <!-- will be generated with JS -->
 +
  </aside>
 +
  </aside>
 +
 
 +
  <main class="post-content">
 +
<!---
 +
    <div class="row">
 +
    <div class="col-12 text-center">
 +
    <figure class="figure" >
 +
    <img src="http://2018.igem.org/wiki/images/2/2b/T--Munich--MS_3S_phage.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
    <figcaption class="figure-caption">BIG BROTHER IS WATCHING YOU</figcaption>
 +
    </figure>
 +
    </div>
 +
    </div>
 +
--->
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<h2></h2>
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<ul style="">
 +
 
 +
</ul>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<ul style="">
 +
 
 +
</ul>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<p>
 +
<br>
 +
</p>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<ul style=""></ul>
 +
</div>
 +
</div>
 +
<!---
 +
--->
 +
 
 +
 
 +
<hr>
 +
 
 +
<h3>
 +
</h3>
 +
 
 +
    <div class="row">
 +
    <div class="col-12 col-md-6">
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
    </figure>
 +
    </div>
 +
    </div>
 +
 
 +
    <div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
 
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
  <figure class="figure">
 +
    <img src="" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
    <figcaption class="figure-caption"></figcaption>
 +
    </figure>
 +
    </div>
 +
    </div>
 +
 
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<hr>
 +
 
 +
<h3>
 +
</h3>
 +
<h5>Sonication</h5>
 +
<div class="row">
 +
<div class="col-12">
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
 
 +
<figure class="figure">
 +
   
 +
</figure>
 +
 
 +
    </div>
 +
    </div>
 +
 
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col-12 col-md-6">
 +
<figure class="figure">
 +
 
 +
</figure>
 +
</div>
 +
<div class="col-12 col-md-6">
 +
<figure class="figure">
 +
</figure>
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
    <div class="col-12">
 +
 
 +
 
 +
    <h5></h5>
 +
    <div class="row">
 +
    <div class="col-12">
 +
 
 +
    </div>
 +
    </div>
 +
 
 +
 
 +
    <div class="row">
 +
        <div class="col-12 col-md-6">
 +
        <figure class="figure">
 +
        <img src="" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
        </figure>
 
         </div>
 
         </div>
 +
        <div class="col-12 col-md-6">
 +
        <figure class="figure">
 +
        <img src="" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
        </figure>
 +
        </div>
 +
    </div>
  
−
         <div class="row mt-4" style="">
+
    <div class="row">
−
         
+
    <div class="col-12">
 +
         <figcaption class="figure-caption"></figcaption>
 +
    </div>
 +
    </div>
 +
 
 +
    <div class="row">
 +
    <div class="col-12">
 +
   
 +
    </div>
 +
    </div>
 +
 
 +
    <h5>Cell Extract Processing</h5>
 +
    <div class="row">
 +
    <div class="col-12">
 +
   
 +
    </div>
 +
    </div>
 +
    <div class="row">
 +
    <div class="col-12">
 +
    <ul style="">
 +
   
 +
    </ul>
 +
    </div>
 +
    </div>
 +
 
 +
<hr>
 +
 
 +
<h3> </h3>
 +
 
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col-12 col-md-6">
 +
 
 +
</div>
 +
<div class="col-12 col-md-6">
 +
  <figure class="figure">
 +
 
 +
  </figure>
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col-12 col-md-6">
 +
 
 +
</div>
 +
<div class="col-12 col-md-6">
 +
 
 +
</div>
 +
</div>
 +
 
 +
<h3>
 +
</h3>
 +
 
 +
 
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
   
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
   
 +
    </figure>
 +
    </div>
 +
    </div>
 +
 
 +
 
 +
<div class="row">
 +
    <div class="col-12 col-md-4">
 +
<figure class="figure">
 +
   
 +
        </figure>
 +
</div>
 +
    <div class="col-12 col-md-4">
 +
  <figure class="figure">
 +
   
 +
      </figure>
 +
    </div>
 +
  <div class="col-12 col-md-4">
 +
  <figure class="figure">
 +
   
 +
      </figure>
 +
    </div>
 +
   
 +
    </div>
 +
 
 +
<hr>
 +
 
 +
<h3>
 +
 
 +
</h3>
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
<ul style="">
 +
</ul>
 +
<br>
 +
<br>
 +
</div>
 +
</div>
 +
 
 +
    <div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
<ul style="">
 +
 
 +
</ul>
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
    </figure>
 +
    </div>
 +
    </div>
 +
 
 +
<h2>
 +
</h2>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<p></p>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
 +
<ul style="">
 +
 
 +
</ul>
 +
</div>
 +
</div>
 +
<h3>
 +
 
 +
</h3>
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
</div>
 +
<div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
 
 +
</div>
 +
<div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
    </figure>
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
<h3>
 +
 
 +
</h3>
 +
 
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
    </div>
 +
    </div>
 +
 
 +
 
 +
<hr>
 +
 
 +
<h3>
 +
 
 +
</h3>
 +
 
 +
<div class="row">
 +
<div class="col-12">
 +
<p>
 +
 
 +
</p>
 +
<br>
 +
<br>
 +
</div>
 +
</div>
 +
 
 +
    <div class="row">
 +
    <div class="col-12 col-md-6">
 +
    </div>
 +
    <div class="col-12 col-md-6">
 +
 
 +
   
 +
    </div>
 +
    </div>
 +
 
 +
 
 +
<div class="row">
 +
<div class="col-12 col-md-3">
 +
  <figure class="figure">
 +
     
 +
      </figure>
 +
</div>
 +
<div class="col-12 col-md-3">
 +
  <figure class="figure">
 +
     
 +
      </figure>
 +
</div>
 +
<div class="col-12 col-md-3">
 +
  <figure class="figure">
 +
     
 +
      </figure>
 +
</div>
 +
<div class="col-12 col-md-3">
 +
  <figure class="figure">
 +
     
 +
      </figure>
 +
</div>
 +
</div>
 +
 
 +
<div class="row">
 +
  <div class="col-12 col-md-3">
 +
    <figure class="figure">
 +
       
 +
        </figure>
 +
  </div>
 +
  <div class="col-12 col-md-3">
 +
    <figure class="figure">
 +
       
 +
        </figure>
 +
  </div>
 +
  <div class="col-12 col-md-3">
 +
    <figure class="figure">
 +
       
 +
        </figure>
 +
  </div>
 +
  <div class="col-12 col-md-3">
 +
  <figure class="figure">
 +
 
 +
  </figure>
 +
</div>
 +
</div>
 +
<div class="row">
 +
<div class="col-12">
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<hr>
 +
 
 +
<div class="row">
 +
    <div class="col-12">
 +
 
 +
    </div>
 +
    </div>
 +
 
 +
    <div class="row">
 +
        <div class="col-12 col-md-6">
 +
    <figure class="figure">
 +
       
 +
            </figure>
 +
    </div>
 +
        <div class="col-12 col-md-6">
 +
      <figure class="figure">
 +
       
 +
          </figure>
 +
        </div>
 
         </div>
 
         </div>
  
−
     </div>  
+
 
−
<div>
+
 
−
<h3>Results</h3>
+
<h5></h5>
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
 
 +
     </div>
 +
    <div class="col-12 col-md-6">
 +
<figure class="figure">
 +
   
 +
    </figure>
 +
    </div>
 +
    </div>
 +
 
 +
<h2>
 +
 
 +
</h2>
 +
<div class="row" style="background-color: rgba(200,200,200,1);">
 +
<div class="col-12">
  
 
</div>
 
</div>
−
<div class="showCase container-fluid jumbotron jumbotron-fluid text-center bg-white align-items-center text-light mb-0">
+
</div>
−
        <div class="row" style="">
+
<h3>
−
            <div class="col-12 col-md-6">
+
 
−
               
+
</h3>
−
            </div>
+
 
−
            <div class="col-12 col-md-6 p-5">
+
 
−
               
+
<div class="row">
−
                 <span class="d-block mt-5">
+
<div class="col-12 col-md-6">
−
               
+
    <figure class="figure">
−
             
+
       
−
               
+
        </figure>
−
             </div>
+
</div>
 +
<div class="col-12 col-md-6">
 +
 
 +
</div>
 +
</div>
 +
 
 +
    </div>
 +
    </div>
 +
 
 +
    <div class="row mt-3">
 +
    <div class="col-12">
 +
    <div id="accordion">
 +
      <div class="card" style="background-color:#017FD4;">
 +
        <div class="card-header" id="headingOne">
 +
          <h5 class="mb-0">
 +
            <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseOne" aria-expanded="false" aria-controls="collapseOne">
 +
                 <span class="pl-3" style="color: white;">Protein Purification</span>
 +
             </button>
 +
          </h5>
 
         </div>
 
         </div>
 +
      <div id="collapseOne" class="collapse" aria-labelledby="headingOne" data-parent="#accordion">
 +
          <div class="card-body bg-white">
 +
              <figure class="figure">
 +
       
 +
          </figure>
  
−
        <div class="row mt-4" style="">
+
          </div>
−
         
+
 
         </div>
 
         </div>
 +
      </div>
  
−
     </div>  
+
          </div>
−
<div class="showCase container-fluid jumbotron jumbotron-fluid text-center bg-white align-items-center text-light mb-0">
+
        </div>
−
         <div class="row" style="">
+
      </div>
−
            <div class="col-12 col-md-6">
+
 
−
               
+
     <div class="row">
−
            </div>
+
    <div class="col-12">
−
            <div class="col-12 col-md-6 p-5">
+
 
−
               
+
  </div>
−
                <span class="d-block mt-5">
+
    </div>
−
               
+
 
−
             
+
    <div class="row mt-3">
−
               
+
    <div class="col-12">
 +
    <div id="accordion">
 +
      <div class="card" style="background-color:#017FD4;">
 +
        <div class="card-header" id="headingTwo">
 +
          <h5 class="mb-0">
 +
            <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo">
 +
                <span class="pl-3" style="color: white;">Protein Characterization</span>
 +
            </button>
 +
          </h5>
 +
        </div>
 +
      <div id="collapseTwo" class="collapse" aria-labelledby="headingTwo" data-parent="#accordion">
 +
          <div class="card-body bg-white">
 +
    <figure class="figure">
 +
       
 +
        </figure>
 +
              <figure class="figure">
 +
       
 +
        </figure>
 +
          </div>
 +
          <div class="card-body bg-white">
 +
              <div class="col-12 col-md-6">
 +
         
 +
              </figure>
 +
              </div>
 +
              </div>
 +
         </div>
 +
      </div>
 +
 
 +
          </div>
 +
        </div>
 +
      </div>
 +
 
 +
<div class="row">
 +
    <div class="col-12 col-md-6">
 +
 
 +
    </div>
 +
    <div class="col-12 col-md-3">
 +
<figure class="figure">
 +
   
 +
    </div>
 +
    <div class="col-12 col-md-3">
 +
<figure class="figure">
 +
   
 +
    </figure>
 +
    </div>
 +
    </div>   
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<h5>
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Bacteriophages From Phactory Assemble And Are Fully Functional
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<p>Discussion goes here
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<figure class="figure">
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    <img src="http://2018.igem.org/wiki/images/7/7f/T--Munich--Results_QPCR_Nils.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
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    <figcaption class="figure-caption">qpcr</figcaption>
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RNA-Detection via RT-qPCR
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<p>
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To rapidly detect functionality of the phages, reverse transcription-quantitative PCR (RT-qPCR) was applied to pellets of T7-infected host cells at different timepoints. The RT-qPCR protocol was used. Using the delta-delta-Ct method3, relative expression was determined and normalized to the value at 3 minutes after addition of the phages. Whereas expression of E. coli genes remains stable, expression of three T7 genes is elevated throughout the experiment. The change of expression of all three phage genes reaches a first peak after 12 minutes and a second peak after 18 or 21 minutes.
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    <p>
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        The sharp increase of phage gene expression in the first phase of the experiment displays the ability of the phages to successfully infect the bacteria and initiate reproduction. The second and even higher increase of expression is likely attributed to a second wave of infection of previously replicated phages. This indicates that the phages are capable of reproducing inside their host bacteria, resulting in multiplication of functional phages.
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The increase of expression of T7P01 is more pronounced than that of T7P07, which is in turn is stronger than that of T7P29. This circumstance is likely caused by differences in primer efficacy, a value describing the doubling rate in between every PCR cycle. For better reliability of the RT-qPCR quality control, primer efficacy should be assessed by creating a standard curve.
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<p>All RNA samples were pooled and checked for DNA contamination, which could have interfered with the RT-qPCR. Absence of DNA was determined electrophoresis in a  gel electrophoresis</p>
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            <th scope="row">T7</th>
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            <td>12h 02min</td>
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            <td>424,198</td>
 +
            <td>1.27 &times; 10<sup>9</sup></td>
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            <th scope="row">3S</th>
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            <td>3h 42min</td>
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            <td>77,092</td>
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            <td>2.53 &times; 10<sup>8</sup></td>
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            <th scope="row">NES</th>
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            <td>3h 58min</td>
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            <td>39,501</td>
 +
            <td>2.31 &times; 10<sup>8</sup></td>
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        </tr>
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            <th scope="row">NFFP</th>
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            <td>5h 42min</td>
 +
            <td>27,633</td>
 +
            <td>1.23 &times; 10<sup>8</sup></td>
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<h2>References</h2>
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Revision as of 15:33, 28 June 2019










Phactory

Results


    A generic square placeholder image with rounded corners in a figure.

    Sonication
    A generic square placeholder image with rounded corners in a figure.
    A generic square placeholder image with rounded corners in a figure.
    Cell Extract Processing
    T7 12h 02min 424,198 1.27 × 109
    3S 3h 42min 77,092 2.53 × 108
    NES 3h 58min 39,501 2.31 × 108
    NFFP 5h 42min 27,633 1.23 × 108

    References


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