Difference between revisions of "Team:CAU China/Notebook"

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/*  下面是表格方面的布局 有冲突再调整 */
 
/*  下面是表格方面的布局 有冲突再调整 */
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/*  Notebook需要布局操作结束*/
  
 
      
 
      
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<div class = "notebookContainer">
 
<div class = "notebookContainer">
  
−
<div class="customelementM5B" id = "entrydayIA8">
 
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−
<h3> Morning </h3>
 
−
 
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<p>Performed Calibration 1, 2, and 3 according to protocol. All measurements were performed using the plate reader of Seino. </p>
 
−
 
−
<table class="InterlabTable" style = "width: 20%">
 
−
<caption style = "font-size: 1.5em;background-color: #A4C2F4">Table 1. content 96 wells plate cal.1 and cal.2
 
−
</caption>
 
−
<tr><th></th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th><th>6</th><th>7</th><th>8</th><th>9</th><th>10</th><th>11</th><th>12</th></tr>
 
−
<tr><td>A</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                 
 
−
<tr><td>B</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 
−
<tr><td>C</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 
−
<tr><td>D</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 
−
<tr><td>E</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">    dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr><tr>
 
−
<td>F</td><td style="color: green">MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green"> dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 
−
<tr><td>G</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 
−
<tr><td>H</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 
−
</table>
 
−
 
−
 
−
<h5> Calibration 1 </h5>
 
−
<ul>
 
−
<li>L= 100 uL Ludox CL-X (stored at 4C)</li>
 
−
<li>dd= 100 uL ddH20</li>
 
−
<li>Measurement: Abs600, turn off pathlength correction</li>
 
−
</ul>
 
−
 
−
<h5>Calibration 2 </h5>
 
−
<ul>
 
−
<li>MS= 200 ul Microsphere Stock Solution</li>
 
−
<li>dd= 100 uL ddH20</li>
 
−
<li>green= serial dilution was performed with a micropipet from E1,F1,G1,H1 - E11,F11,G11,H11 by a volume of 100uL.            Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged.</li>
 
−
<li>Measurement: Abs600, re-mix befor putting in plate reader and prevent bubbles, path length correction off</li>
 
−
</ul>
 
−
 
−
<h5>Calibration 3</h5>
 
−
<ul>
 
−
<li>1xFC= 200 mL 1xFC (100uL 10x fluorescein + 900ul 1x PBS pH 7.4, tube was covered with foil</li>
 
−
<li>P= 100 uL 1x PBS pH 7.4</li>
 
−
<li>green= serial dilution was performed with a micropipet from A1,B1,C1,D1 - A11,B11,C11,D11 by a volume of 100uL. Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged. </li>
 
−
<li>Measurement: FL, 530nm/30nm bandpass, 25-30nm with recommened excitation of 485nm, emission 520-530nm of the filter. Path length correction was turned off</li>
 
−
</ul>
 
−
 
−
<table class="InterlabTable" style = "width: 20%">
 
−
<caption style = "font-size: 1.5em;background-color: #A4C2F4">Table 2. content 96 wells plate cal.3</caption>
 
−
<tr><th></th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th><th>6</th><th>7</th><th>8</th><th>9</th><th>10</th><th>11</th><th>12</th></tr>
 
−
<tr><td>A</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                 
 
−
<tr><td>B</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 
−
<tr><td>C</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 
−
<tr><td>D</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 
−
<tr><td>E</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 
−
<tr><td>F</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 
−
<tr><td>G</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 
−
<tr><td>H</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 
−
</table>
 
−
 
−
<h3> Afternoon </h3>
 
−
 
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<h5>LBC plates were made according to the protocol used on the wall</h5>
 
−
<ul>
 
−
<li>250ml LB 2x added to melted 250 ml WA 2x using a microwave </li>
 
−
<li>0.5ml was added to final solution</li>
 
−
<li>plates were dried in 37C incubator</li>
 
−
</ul>
 
−
 
−
<h5>Transformation device 3 + negative control interlab study</h5>
 
−
<ul>
 
−
<li>Device 3 (number 5) showed a low GFP expression, so it was tried to re-preform the tranformation. Negative control of the interlab (number 1) was not performed last time due to lack of LBC plates so was also performed. </li>
 
−
<li><a href = "http://2018.igem.org/Team:Utrecht/Protocol#Transformation">Protocol Transformation</a></li>
 
−
</ul>
 
−
</div>
 
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<div class="customelementM5B" id = "entrydayIJ2">
 
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</div>
 
  
 
</div>
 
</div>

Revision as of 08:40, 8 August 2019










Phactory

Astaxanthin

May
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Cellulose

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