Difference between revisions of "Team:CAU China/Notebook"

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@media only screen and (max-width: 1199.98px) {}
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<!--<p class="codyhouse">link to the site of Codyhouse: <a href="https://codyhouse.co/gem/schedule-template/">https://codyhouse.co/gem/schedule-template/</a></p>-->
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<!-- Tab links -->
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  <button id = "tabR" onclick='changeContent(2)'>Astaxanthin</button>
 +
  <button id = "tabB" onclick='changeContent(3)'>Cellulose</button>
  
 
</div>
 
</div>
  
 +
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−
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<!-- 虾青素的分组显示--------------------------------------------------------------
−
    <div class="col-12 text-center">
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--------------------------------------------------------------------------------------------->
−
<div class="mt-2"><a class="PDFlinks text-center" target="_blank" href="">Primer List</a></div>
+
<div id="monthHeaderR" class="monthHeader">
 +
<h1> Astaxanthin </h1>
 +
 
 +
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    <div>October</div>
 +
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 +
 +
 +
</div>
 +
 +
<!-- 纤维素的月份格式---------------------------------------------------------------------
 +
--------------------------------------------------------------------------------------------->
 +
 +
<div id="monthHeaderB" class="monthHeader">
 +
<h1> Cellulose</h1>
 +
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 +
    <div onclick="createMonth(31, 1, inactiveJuly, 'J2');" class="next">&#10095;</div>
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 +
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</div>
 +
 +
 +
<!-- Calender Methylation Assay --------------------------------------------------------------
 +
--------------------------------------------------------------------------------------------->
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  <div class="weekday">Mo</div>
 +
  <div class="weekday">Tu</div>
 +
  <div class="weekday">We</div>
 +
  <div class="weekday">Th</div>
 +
  <div class="weekday">Fr</div>
 +
  <div class="weekday">Sa</div>
 +
  <div class="weekday">Su</div>
 +
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<div class="monthContainer"></div>
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<!-- Notebook Interlab -----------------------------------------------------------------------
 +
--------------------------------------------------------------------------------------------->
 +
 +
<div class = "notebookContainer">
 +
 +
<div class="customelementM5B" id = "entrydayIA8">
 +
 +
<h3> Morning </h3>
 +
 +
<p>Performed Calibration 1, 2, and 3 according to protocol. All measurements were performed using the plate reader of Seino. </p>
 +
 +
<table class="InterlabTable" style = "width: 20%">
 +
<caption style = "font-size: 1.5em;background-color: #A4C2F4">Table 1. content 96 wells plate cal.1 and cal.2
 +
</caption>
 +
<tr><th></th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th><th>6</th><th>7</th><th>8</th><th>9</th><th>10</th><th>11</th><th>12</th></tr>
 +
<tr><td>A</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                 
 +
<tr><td>B</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 +
<tr><td>C</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 +
<tr><td>D</td><td>L</td><td>dd</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>                             
 +
<tr><td>E</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">    dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr><tr>
 +
<td>F</td><td style="color: green">MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green"> dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 +
<tr><td>G</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 +
<tr><td>H</td><td style="color: green"> MS</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td style="color: green">dd</td><td>dd</td></tr>
 +
</table>
 +
 +
 +
<h5> Calibration 1 </h5>
 +
<ul>
 +
<li>L= 100 uL Ludox CL-X (stored at 4C)</li>
 +
<li>dd= 100 uL ddH20</li>
 +
<li>Measurement: Abs600, turn off pathlength correction</li>
 +
</ul>
 +
 +
<h5>Calibration 2 </h5>
 +
<ul>
 +
<li>MS= 200 ul Microsphere Stock Solution</li>
 +
<li>dd= 100 uL ddH20</li>
 +
<li>green= serial dilution was performed with a micropipet from E1,F1,G1,H1 - E11,F11,G11,H11 by a volume of 100uL.            Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged.</li>
 +
<li>Measurement: Abs600, re-mix befor putting in plate reader and prevent bubbles, path length correction off</li>
 +
</ul>
 +
 +
<h5>Calibration 3</h5>
 +
<ul>
 +
<li>1xFC= 200 mL 1xFC (100uL 10x fluorescein + 900ul 1x PBS pH 7.4, tube was covered with foil</li>
 +
<li>P= 100 uL 1x PBS pH 7.4</li>
 +
<li>green= serial dilution was performed with a micropipet from A1,B1,C1,D1 - A11,B11,C11,D11 by a volume of 100uL. Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged. </li>
 +
<li>Measurement: FL, 530nm/30nm bandpass, 25-30nm with recommened excitation of 485nm, emission 520-530nm of the filter. Path length correction was turned off</li>
 +
</ul>
 +
 +
<table class="InterlabTable" style = "width: 20%">
 +
<caption style = "font-size: 1.5em;background-color: #A4C2F4">Table 2. content 96 wells plate cal.3</caption>
 +
<tr><th></th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th><th>6</th><th>7</th><th>8</th><th>9</th><th>10</th><th>11</th><th>12</th></tr>
 +
<tr><td>A</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                 
 +
<tr><td>B</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 +
<tr><td>C</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 +
<tr><td>D</td><td>1xFC</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td><td>P</td></tr>                             
 +
<tr><td>E</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 +
<tr><td>F</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 +
<tr><td>G</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 +
<tr><td>H</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>
 +
</table>
 +
 +
<h3> Afternoon </h3>
 +
 +
<h5>LBC plates were made according to the protocol used on the wall</h5>
 +
<ul>
 +
<li>250ml LB 2x added to melted 250 ml WA 2x using a microwave </li>
 +
<li>0.5ml was added to final solution</li>
 +
<li>plates were dried in 37C incubator</li>
 +
</ul>
 +
 +
<h5>Transformation device 3 + negative control interlab study</h5>
 +
<ul>
 +
<li>Device 3 (number 5) showed a low GFP expression, so it was tried to re-preform the tranformation. Negative control of the interlab (number 1) was not performed last time due to lack of LBC plates so was also performed. </li>
 +
<li><a href = "http://2018.igem.org/Team:Utrecht/Protocol#Transformation">Protocol Transformation</a></li>
 +
</ul>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayIJ2">
 +
lskdfjslkjgdlkdfjgldkjlakmflsvmclkxmfklmgdmlkcvmblcmkb
 +
</div>
 +
 +
</div>
 +
 +
<!-- Notebook Astaxanthin --------------------------------------------------------------
 +
--------------------------------------------------------------------------------------------->
 +
<div class="customelementM5B" id = "entrydayRM22">
 +
  <h2>22/05/19</h2>
 +
  <b>Name</b>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRM28">
 +
  <h2>28/05/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRM29">
 +
<h2>29/05/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRM29">
 +
<h2>29/05/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ103">
 +
<h2>03/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ104">
 +
<h2>04/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ105">
 +
<h2>05/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
<div class="customelementM5B" id = "entrydayRJ106">
 +
<h2>06/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ108">
 +
<h2>08/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ127">
 +
<h2>27/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ129">
 +
<h2>29/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayRJ130">
 +
<h2>30/06/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
<!--- 目前还没有加入进表格的记录 --->
 +
<div class="customelementM5B" id = "entrydayRJ2">
 +
<h2>/07/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
 +
<div class="customelementM5B" id = "entrydayRA">
 +
<h2>/08/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
 +
<div class="customelementM5B" id = "entrydayRS">
 +
<h2>/09/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
 +
 +
<div class="customelementM5B" id = "entrydayRO">
 +
<h2>/10/19</h2>
 +
  <b>Name</b>
 +
 +
</div>
 +
 +
 +
 +
 +
<!-- Notebook Cellulose --------------------------------------------------------------
 +
--------------------------------------------------------------------------------------------->
 +
<div class="customelementM5B" id = "entrydayBJ16">
 +
<h2>06/06/18</h2>
 +
<b>Name</b>
 +
 +
</div>
 +
<div class="customelementM5B" id = "entrydayBJ21">
 +
<h2>01/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
 +
<div class="customelementM5B" id = "entrydayBJ22">
 +
<h2>02/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
 +
<div class="customelementM5B" id = "entrydayBJ28">
 +
<h2>08/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayBJ29">
 +
<h2>09/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayBJ210">
 +
<h2>10/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayBJ211">
 +
<h2>11/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
<div class="customelementM5B" id = "entrydayBJ216">
 +
<h2>16/07/18</h2>
 +
<b>Name</b>
 +
</div>
 +
 +
 +
 +
</body>
 +
 +
 +
 +
 +
 +
 +
  
 
<script>
 
<script>

Revision as of 07:44, 8 August 2019










Phactory

Notebook

Astaxanthin

May
June
July
August
September
October

Cellulose

June
July
August
September
October
Mo
Tu
We
Th
Fr
Sa
Su

Morning

Performed Calibration 1, 2, and 3 according to protocol. All measurements were performed using the plate reader of Seino.

Table 1. content 96 wells plate cal.1 and cal.2
123456789101112
ALdd
BLdd
CLdd
DLdd
E MSdddddddddddd dddddddddd
FMSdddddddddddd dddddddddd
G MSdddddddddddddddddddddd
H MSdddddddddddddddddddddd
Calibration 1
  • L= 100 uL Ludox CL-X (stored at 4C)
  • dd= 100 uL ddH20
  • Measurement: Abs600, turn off pathlength correction
Calibration 2
  • MS= 200 ul Microsphere Stock Solution
  • dd= 100 uL ddH20
  • green= serial dilution was performed with a micropipet from E1,F1,G1,H1 - E11,F11,G11,H11 by a volume of 100uL. Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged.
  • Measurement: Abs600, re-mix befor putting in plate reader and prevent bubbles, path length correction off
Calibration 3
  • 1xFC= 200 mL 1xFC (100uL 10x fluorescein + 900ul 1x PBS pH 7.4, tube was covered with foil
  • P= 100 uL 1x PBS pH 7.4
  • green= serial dilution was performed with a micropipet from A1,B1,C1,D1 - A11,B11,C11,D11 by a volume of 100uL. Before every transfer solution was pipetted up and down 3x, after every transfer tips were discharged.
  • Measurement: FL, 530nm/30nm bandpass, 25-30nm with recommened excitation of 485nm, emission 520-530nm of the filter. Path length correction was turned off
Table 2. content 96 wells plate cal.3
123456789101112
A1xFCPPPPPPPPPPP
B1xFCPPPPPPPPPPP
C1xFCPPPPPPPPPPP
D1xFCPPPPPPPPPPP
E
F
G
H

Afternoon

LBC plates were made according to the protocol used on the wall
  • 250ml LB 2x added to melted 250 ml WA 2x using a microwave
  • 0.5ml was added to final solution
  • plates were dried in 37C incubator
Transformation device 3 + negative control interlab study
  • Device 3 (number 5) showed a low GFP expression, so it was tried to re-preform the tranformation. Negative control of the interlab (number 1) was not performed last time due to lack of LBC plates so was also performed.
  • Protocol Transformation
lskdfjslkjgdlkdfjgldkjlakmflsvmclkxmfklmgdmlkcvmblcmkb

22/05/19

Name

28/05/19

Name

29/05/19

Name

29/05/19

Name

03/06/19

Name

04/06/19

Name

05/06/19

Name

06/06/19

Name

08/06/19

Name

27/06/19

Name

29/06/19

Name

30/06/19

Name

/07/19

Name

/08/19

Name

/09/19

Name

/10/19

Name

06/06/18

Name

01/07/18

Name

02/07/18

Name

08/07/18

Name

09/07/18

Name

10/07/18

Name

11/07/18

Name

16/07/18

Name


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