Our models are mainly used to help us design and predict the whole project. Firstly, to find out which pathway is worth to be constructed in our project, we conducted an AHP model, which is a decision model used in pre-project process. By using AHP, we decide to make an Astaxanthin pathway in E.coli. Secondly, in order to help experiment find the suitable experiment condition, we work out an Logistic Regression to predict the E.coli population. By doing this, we successfully find the most suitable experiment bacterial concentration. Finally, our team use System Dynamic Model(SDM) to control our pathway and predict the final product. With such a model, we can give the experiment designing process a huge inspiration. The total modeling project based on the sight that we have to use mathematic methods to help our project and surely we reach the goal!