Difference between revisions of "Team:Bioriidl Somaiya/Human Practices"

 
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Latest revision as of 21:49, 21 October 2019

iGEM | Bioriidl

Primary Human Practices

Why human practice?

It is an essential part of the project by which the problem statement is justified & it gives data on my problems related to autoclave & its positive points. The statements vary from field to field. Human practices have done in 3 parts:

Impact

The impact on using an autoclave for different purposes by different people. How autoclave used & what was the purpose of using an autoclave. To show how sterility is important in the experiment. The importance of sterile equipment.

Autoclave Benefits

  1. Sterility
  2. Sterile Glassware
  3. Sterile Media
  4. Sterile Chemical

Importance Of Sterility

  • Sterile zone
  • Sterile equipment in ATC, PTC
  • No contamination

Strategy

The community was formed of different backgrounds with the same field. The questions were asked to groups were:

How much you have experience of using autoclave?

How much time does it take for autoclaving?

How much quantity of lab equipment, media, chemical?

What sterilization method do you use?

What is your purpose of sterilization?

What are the issues with the autoclave?

What are the upgrades do you want to see in an autoclave?

Do you want some other way of autoclave?

Will you prefer another way or process of autoclaving?

What are your ideas to put new innovative idea forsterilization?

Goals

The approach was to see that the autoclave machine which is used does it need an upgrade.

The current autoclave which is used does it has a drawback

To find some new technique for sterilization

To find new upgrades for the autoclave

Groups

The groups were made with people from the same background & work

Lab Assistants

+

Professors

+

Student

+

Startup

+

Researchers

+

Industry

+
×

Lab assistants: from different fields like Biotech, Microbiology, Zoology, etc.

Why we approached them?

These are people who give us the glassware, media, chemical which are sterilized. They sterilize the media, glassware, chemical in 2 batches per day. That includes chemical, glassware, media

We learn that the process:

It was very time consuming, there were many precautions like water level, pressure exerting mechanism, screws to be tightened before the use.

What upgrade needs to be done:

The portability of autoclaves should be done. The time consumption should be less. It should sterilize in a continuous process with different & continues series of glassware, chemical, media. Safety measures should be seen in precautions like low water level, pressure.

×

Professors: The professors which we asked were from a biology background with different pure biology field.

Why we approached them?

They were the teacher who taught us how to use an autoclave, why it is needed. They use an autoclave for academic purposes & their own research.

What we learn:

The time-consuming process of an autoclave. The precaution of water level, pressure output while taking out the equipment the hand gets burned.

What upgrade needs to be done:

The portable autoclave should be made. It should be innovated by a new system of sterilization. It should be usable in an open field.

×

Student: These are the group. Which used an autoclave for academic purposes. They were taught by the teacher in practicals etc.

Why we approached them?

They use the autoclave for the practical. To know their view on the autoclave machine.

What we learn:

The first appearance of an autoclave is like steel can. It is hard & scary to operate the machine. The stink of discard Petri plates gives a bad smell. The noise created by the autoclave. Hand burn during media pouring.

What upgrade needs to be done:

It should be easy to use for understanding. The machine should not create noise & smell. It should be easy to carry. Less time should be used. There should be automated suspension of media.

×

Startup: These guys used sterilization in the product & raw material. They used sterilization in the process.

Why we approached them?

They use this for sterilization techniques for research. The use of sterilization for sterilizing media in mass, cockpit, etc. They use different sterilization techniques like vapor.

What we learn:

The different types of sterilization like vapor, hydrogen peroxide, etc. The impact of sterility in a biological product such as enzyme, protein, etc.

What upgrade needs to be done:

It should be easy to maintain. It should be cheap & portable. The machine should be made according to the specificity of the product.

×

Researchers: They do research in the specific field & specific subject. The research done is specific to medical, environmental, agricultural, etc.

Why we approached them?

They used sterile chemicals, media, glassware for research. The different sterilization techniques for different media example heat-labile, nanopore media, etc. The sampling technique in the different research.

What we learn:

We learn different types of sterilization like gamma sterilization. We came to know about the filtration technique. The need for sterilized media, glassware, etc.in urgent.

What upgrade needs to be done:

Portability of autoclave machine. Time consumption should be less. The flash sterilization technique should be there.

×

Industry: The industry like pharmaceutical, biotech industry, etc.

Why we approached them?

They used to sterilization for product making and the process. They use autoclave industrial processes like alcohol formation, antibody formation, vaccine preparation, etc. The do sterilization of bags, syringe, bottles, etc.

What we learn:

We learn different materials are sterilized for packaging like bottles, syringes. The quantity or batch sterilization process. The importance of sterile packaging.

What upgrade needs to be done:

Cost-effective mass sterilization. Batch sterilization should take less timing. The weight should be less of an autoclave for moving transportation.

Integrated Human practices

The starport which we are making contains the upgrades according to question which was asked verbally & google form was circulated. We got some of the upgrades which we have by the data we have collected. The up-gradation points we have:

  1. We take less time consumption for the whole process takes 8 to 10 minutes.
  2. We have used heating & UV radiation for sterilization.
  3. It does not create noise disturbance.
  4. It has all the precautions measures for UV.
  5. The steam is not produced.
  6. It is portable & easy to move. It has less weight.
  7. It is easy to use.
  8. The appearance of machine is good.
  9. It has suspension system form media pouring integrated with machine

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