Difference between revisions of "Team:Bioriidl Somaiya/Design"

 
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<html lang="en">
 
<html lang="en">
 
   <head>
 
   <head>
 +
<link
 +
      href="https://stackpath.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css"
 +
      rel="stylesheet"
 +
      integrity="sha384-wvfXpqpZZVQGK6TAh5PVlGOfQNHSoD2xbE+QkPxCAFlNEevoEH3Sl0sibVcOQVnN"
 +
      crossorigin="anonymous"
 +
    />
 +
    <link
 +
      rel="stylesheet"
 +
      href="https://maxcdn.bootstrapcdn.com/bootstrap/3.4.0/css/bootstrap.min.css"
 +
    />
 +
    <link
 +
      href="https://stackpath.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css"
 +
      rel="stylesheet"
 +
      integrity="sha384-wvfXpqpZZVQGK6TAh5PVlGOfQNHSoD2xbE+QkPxCAFlNEevoEH3Sl0sibVcOQVnN"
 +
      crossorigin="anonymous"
 +
    />
 +
    <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.4.1/jquery.min.js"></script>
 +
    <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script>
 +
    <script src="https://maxcdn.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>
 
     <meta charset="UTF-8" />
 
     <meta charset="UTF-8" />
 
     <meta name="viewport" content="width=device-width, initial-scale=1.0" />
 
     <meta name="viewport" content="width=device-width, initial-scale=1.0" />
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             background: linear-gradient(#111116, #0b0c24);
 
             background: linear-gradient(#111116, #0b0c24);
 
         }
 
         }
 +
      /* Scrollbar customized */
 +
 +
      ::-webkit-scrollbar {
 +
        width: 12px;
 +
      }
 +
 +
      ::-webkit-scrollbar-track {
 +
        box-shadow: inset 0 0 5px #00695c;
 +
        background: #ddd;
 +
      }
 +
 +
      ::-webkit-scrollbar-thumb {
 +
        background: linear-gradient(to bottom, #007722, #00aa55);
 +
        border-radius: 3px;
 +
        box-shadow: 2px 2px 4px grey;
 +
      }
 +
 +
      ::-webkit-scrollbar-thumb:hover {
 +
        background: linear-gradient(to bottom, #7d1b54, #e6369c);
 +
        border: 2px solid #75194f;
 +
      }
 +
 +
            a {
 +
        text-decoration: none;
 +
      }
 +
 
       /* Scrollbar customized */
 
       /* Scrollbar customized */
  
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         margin: 0;
 
         margin: 0;
 
         position: sticky;
 
         position: sticky;
−
      }
+
        background: #fff;
−
 
+
         border-bottom: 1px solid #444;
−
      .navbar-nav {
+
−
         margin: 0;
+
 
       }
 
       }
  
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       }
 
       }
  
−
       .nav-transparent {
+
       .dropdown-menu li {
−
         transition: 0.3s;
+
         min-width: 20vw;
 
       }
 
       }
  
−
       .nav-colored {
+
       .dropdown-menu > li > a {
−
         background-color: #fff;
+
         font-size: 16px;
−
         border-bottom: 1px solid #444;
+
         padding: 2vh 2vw;
−
         transition: 0.3s;
+
         color: #777;
 
       }
 
       }
  
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         .navbar-nav {
 
         .navbar-nav {
 
           background: #fff;
 
           background: #fff;
 +
        }
 +
 +
        .dropdown-menu li {
 +
          min-width: 60vw;
 +
        }
 +
 +
        .dropdown-menu > li > a {
 +
          padding: 3vh 8vw;
 
         }
 
         }
 
       }
 
       }
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             data-target="#myNavbar"
 
             data-target="#myNavbar"
 
           >
 
           >
−
             <img src="https://2019.igem.org/wiki/images/1/1b/T--Bioriidl_Somaiya--ham.png" alt="" style="margin-top: 3px;" />
+
             <img
 +
              src="https://2019.igem.org/wiki/images/1/1b/T--Bioriidl_Somaiya--ham.png"
 +
              alt=""
 +
              style="margin-top: 3px;"
 +
            />
 
           </button>
 
           </button>
−
           <a class="navbar-brand" href="#homeSection">
+
           <a
−
             <img src="https://2019.igem.org/wiki/images/d/de/T--Bioriidl_Somaiya--igem.png" alt="igem logo" />
+
            class="navbar-brand"
 +
            href="https://2019.igem.org/Team:Bioriidl_Somaiya"
 +
          >
 +
             <img
 +
              src="https://2019.igem.org/wiki/images/d/de/T--Bioriidl_Somaiya--igem.png"
 +
              alt="igem logo"
 +
            />
 
           </a>
 
           </a>
 
         </div>
 
         </div>
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         >
 
         >
 
           <ul class="nav navbar-nav">
 
           <ul class="nav navbar-nav">
−
             <li><a href="#about">About</a></li>
+
             <li class="dropdown">
−
             <li><a href="#eventOverview">Project</a></li>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#"
−
             <li><a href="#campusAmba">Parts</a></li>
+
                >Team <span class="caret"></span
−
             <li><a href="#campusAmba">Result</a></li>
+
              ></a>
−
             <li><a href="#contact">Human Practices</a></li>
+
              <ul class="dropdown-menu">
−
             <li><a href="#contact">Medal Criteria</a></li>
+
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Team'; "
 +
                >
 +
                  <a href="#">Team Members</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Collaborations'; "
 +
                >
 +
                  <a href="#">Collaboration</a>
 +
                </li>
 +
              </ul>
 +
             </li>
 +
            <li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#"
 +
                >Project <span class="caret"></span
 +
              ></a>
 +
              <ul class="dropdown-menu">
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Description'; "
 +
                >
 +
                  <a href="#">Description</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Design'; "
 +
                >
 +
                  <a href="#">Design</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Experiments'; "
 +
                >
 +
                  <a href="#">Experiments</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Experiments'; "
 +
                >
 +
                  <a href="#">Notebook</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Team'; "
 +
                >
 +
                  <a href="#">Contributions</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Results'; "
 +
                >
 +
                  <a href="#">Results</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Demonstrate'; "
 +
                >
 +
                  <a href="#">Demonstration</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Improve'; "
 +
                >
 +
                  <a href="#">Improvements</a>
 +
                </li>
 +
                <!-- <li onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Attributions'; ">
 +
                  <a href="#">Attributions</a>
 +
                </li> -->
 +
              </ul>
 +
             </li>
 +
            <li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#"
 +
                >Parts <span class="caret"></span
 +
              ></a>
 +
              <ul class="dropdown-menu">
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Parts'; "
 +
                >
 +
                  <a href="#">Parts Overview</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Parts'; "
 +
                >
 +
                  <a href="#">Basic Parts</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Parts'; "
 +
                >
 +
                  <a href="#">Composite Parts</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Parts'; "
 +
                >
 +
                  <a href="#">Parts Collection</a>
 +
                </li>
 +
              </ul>
 +
             </li>
 +
            <li
 +
              onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Safety'; "
 +
            >
 +
              <a href="#">Safety</a>
 +
            </li>
 +
             <li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#"
 +
                >Human Practices <span class="caret"></span
 +
              ></a>
 +
              <ul class="dropdown-menu">
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Human_Practices'; "
 +
                >
 +
                  <a href="#">Human Practices</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Public_Engagement'; "
 +
                >
 +
                  <a href="#">Education and Engagement</a>
 +
                </li>
 +
              </ul>
 +
             </li>
 +
            <li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#"
 +
                >Awards <span class="caret"></span
 +
              ></a>
 +
              <ul class="dropdown-menu">
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Entrepreneurship'; "
 +
                >
 +
                  <a href="#">Entrepreneurship</a>
 +
                </li>
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Hardware'; "
 +
                >
 +
                  <a href="#">Hardware</a>
 +
                </li>
 +
                <!-- <li onclick="window.location= ''; ">
 +
                  <a href="#">Measurement</a>
 +
                </li>
 +
                <li onclick="window.location= ''; "><a href="#">Model</a></li>
 +
                <li onclick="window.location= ''; "><a href="#">Plant</a></li> -->
 +
                <li
 +
                  onclick="window.location= 'https://2019.igem.org/Team:Bioriidl_Somaiya/Software'; "
 +
                >
 +
                  <a href="#">Software</a>
 +
                </li>
 +
              </ul>
 +
            </li>
 +
            <li
 +
              onclick="window.location= 'https://igem.org/2019_Judging_Form?team=Bioriidl_Somaiya'; "
 +
            >
 +
              <a href="#">Judging Form</a>
 +
            </li>
 
           </ul>
 
           </ul>
−
          <!-- <ul class="nav navbar-nav navbar-right">
 
−
                    <li>
 
−
                    <a href="./login/login.html"
 
−
                        ><span class="glyphicon glyphicon-log-in"></span> Login</a
 
−
                    >
 
−
                    </li>
 
−
                </ul> -->
 
 
         </div>
 
         </div>
 
       </div>
 
       </div>

Latest revision as of 21:36, 21 October 2019

iGEM | Bioriidl

Design

The biological design of our project was conducted using various assembly to optimize the functionality of the organism.

Initially we were in searching for the proteins which will act as an antimicrobial peptide, having potential to kill the contaminating bacteria present in the non-sterile media. Thus, this landed us with three main protein that can potentially affect the viability of the contaminants. The following are the description of the three desired proteins.

Nisin A

Normally this Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on the depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. In our project we have used this as an anti-microbial peptide to kill all the bacterial contaminants present in the media.

Mode of action: Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Subtilosin-A

There are two genes used in combination which will help in releasing the bactericidal protein Subtilosin-A. Subtilosin-A has bactericidal activity against some gram-positive bacteria and some gram-negative bacteria. Sbo-A gene is used in combination with the alb-A gene to help in proper maturation and activation of Subtilosin-A protein. As the gene alb-A catalyzes the formation of 3 thioether bonds during the production of the sactipeptide subtilosin from SboA. In vitro the thioether bonds cannot be made in the absence of the SboA propeptide, suggesting this is the first reaction in subtilosin maturation. This has garnered much attention for their use as safe, natural food preservatives, as well as their potential in medical applications. Subtilosin A (commonly referred to as subtilosin) has a cyclical, cross-linked structure unique among characterized bacteriocins.

Mode of Action- This synthesized peptides produced by bacteria that have antimicrobial activity against organisms closely related to the producer species. Due to its ability to be permeabilized into target cell’s cytoplasmic membrane. Electrostatic interactions between the membrane and protein provide a temporary linkage that allows the hydrophobic peptide to insert itself into the membrane. In many cases, these transient pores produce leakage of intracellular ions, amino acids, and other low molecular weight molecules and eventually cause a total depletion of the cell’s transmembrane ion potential.

Sporulation Killing Factor (SKF)

.The gene skfA produces a 26-residue extracellular sporulation killing factor (skfA). The mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine, the thioether bond.

Mode of action- (SKF) that induces the lysis of other B.subtilis cells that have not entered the sporulation pathway. Used in our system to inhibit the spore bearer and to denature them

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