Team:Bio Without Borders/Notebook
Notebook
Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Using DNA distribution kit plates: Rehydration of registry DNA from kit plate
Wells in the DNA distribution kit plates contain 2-3 ng of dry DNA. This should be enough DNA for a few transformation, but insufficient for immediate restriction and ligation.
Protocol
- Locate the well with the DNA part you need
- Using a micro pipette, draw 10 μL sterile distilled water into a sterile pipette tip
- Use the pipette tip to puncture a hole through the cover foil and into the well containing DNA you need.
- Release the water into the well and pipette up and down a few times to rehydrate the DNA you need
- Store the re-hydrated DNA in the -20C freezer. You can either leave it in the original plate, or transfer it into a separate tube (make sure to label the tube!).
- The rehydrated DNA can be used immediately to transform competent cells. As little as 1 μL should be enough for one transformation.
Inspiration
You can see what others teams have done to organize their notes: