What should this page have?

• Chronological notes of what your team is doing.
• Brief descriptions of daily important events.
• Pictures of your progress.
• Mention who participated in what task.

Using DNA distribution kit plates: Rehydration of registry DNA from kit plate

Wells in the DNA distribution kit plates contain 2-3 ng of dry DNA. This should be enough DNA for a few transformation, but insufficient for immediate restriction and ligation.

Materials

• DNA distribution kit plates
• Sterile distilled water
• Micropipette
• Sterile micropipette tip

Protocol

1. Locate the well with the DNA part you need
2. Using a micro pipette, draw 10 μL sterile distilled water into a sterile pipette tip
3. Use the pipette tip to puncture a hole through the cover foil and into the well containing DNA you need.
4. Release the water into the well and pipette up and down a few times to rehydrate the DNA you need
5. Store the re-hydrated DNA in the -20C freezer. You can either leave it in the original plate, or transfer it into a separate tube (make sure to label the tube!).
6. The rehydrated DNA can be used immediately to transform competent cells. As little as 1 μL should be enough for one transformation.