Difference between revisions of "Team:Bio Without Borders/Notebook"

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<li>Mention who participated in what task.</li>
 
<li>Mention who participated in what task.</li>
 
</ul>
 
</ul>
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<h3>Using DNA distribution kit plates: Rehydration of registry DNA from kit plate</h3>
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<p>Wells in the DNA distribution kit plates contain 2-3 ng of dry DNA. This should be enough DNA for a few transformation, but insufficient for immediate restriction and ligation.</p>
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<h4>Protocol</h4>
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<ol>
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<li>Locate the well with the DNA part you need</li>
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<li>Using a micro pipette, draw 10 μL sterile distilled water into a sterile pipette tip</li>
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<li>Use the pipette tip to puncture a hole through the cover foil and into the well containing DNA you need.</li>
 +
<li>Release the water into the well and pipette up and down a few times to rehydrate the DNA you need</li>
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<li>Store the re-hydrated DNA in the -20C freezer. You can either leave it in the original plate, or transfer it into a separate tube (make sure to label the tube!).</li>
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<li>The rehydrated DNA can be used immediately to transform competent cells. As little as 1 μL should be enough for one transformation.</li>
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</ol>
  
 
</div>
 
</div>

Revision as of 20:01, 25 September 2019


HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Notebook
Contribution
Results
Attributions
PARTS
Parts Overview
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
JUDGING FORM ⇗

Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

What should this page have?

  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.

Using DNA distribution kit plates: Rehydration of registry DNA from kit plate

Wells in the DNA distribution kit plates contain 2-3 ng of dry DNA. This should be enough DNA for a few transformation, but insufficient for immediate restriction and ligation.

Protocol

  1. Locate the well with the DNA part you need
  2. Using a micro pipette, draw 10 μL sterile distilled water into a sterile pipette tip
  3. Use the pipette tip to puncture a hole through the cover foil and into the well containing DNA you need.
  4. Release the water into the well and pipette up and down a few times to rehydrate the DNA you need
  5. Store the re-hydrated DNA in the -20C freezer. You can either leave it in the original plate, or transfer it into a separate tube (make sure to label the tube!).
  6. The rehydrated DNA can be used immediately to transform competent cells. As little as 1 μL should be enough for one transformation.

Inspiration

You can see what others teams have done to organize their notes: