Template:Jilin China/Dem C.js
/**
* * @authors lhx * @date 2019-05-30 16:25:38 * @version 1.1 */
/*var content={ part:[ { title:"Welcome to Jilin_China!", para:[
{ type:"word", cont:"Our website is under development.", class:"np3" }, { type:"word", cont:"We have uploaded our Description and Inspiration. click Description&Inspiration or enter from the upper navigation bar project>Description or click menu>description in the lower right corner.", spClass:"Description&Inspiration","em", link:"Description&Inspiration","https://2019.igem.org/Team:Jilin_China/Description", class:"np5" }
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- /
var content_alpha = {
prospect:{
title:"Demonstrate"
},
part: [{
title: "Our Rules and Policies",
para: [
{ type: "word", cont: "We submit the safety form and check in form on time. All of the organisms and parts we used were in the white list except (ita)C. albicans(itaed) and Bcam0581, which have been listed in the check-in form. We used the safe strains which are according with the relevant regulations in experiment and the safety of our experiment has been evaluated by experienced professors.", class: "np5" },
{ type: "word", cont: "All in all, we are definitely sure that all the systems we built and the process of experiments accorded with the iGEM rules and policies.", //spClass:"deprivation","strong", //link:"temperature","www.urlm.com.cn", class: "np5" },
]
},
{
title: "Therapeutic Effect of Project",
para: [{
type: "word",
cont: "The therapeutic system is the key point of our project, of which each component has been verified to have the expected therapeutic effects.",
class: "np5"
},
{
type: "word",
cont: "i. Firstly, the therapeutic system was verified individually. The results showed that each therapeutic part was successfully expressed and carried planned functions. <a href=\"https://2019.igem.org/Team:Jilin_China/Result\">(result)</a>",
class: "np5"
},
{
type: "word",
cont: "ii. In order to prove that our system can work under realistic conditions, we have conducted the following experiments:",
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},
{
type: "word",
cont: "The project constructed composite components of the four therapeutic parts co-expression system (pVE-Bcam0581-Msp1-β-1,3-glucanase-LL-37). However, the verification has not been completely accomplished because of the limited time.",
class: "np5"
},
{
type: "word",
cont: "We took four kinds of supernatant separately and obtained the mixture of them. Instead of co-expression system, we use the mixture to test the effect of inhibiting the growth of (ita)C. albicans(itaed) and the ability to kill them. Present studies have shown that the co-therapeutic effect is better than that based on single therapeutic part. However, the expression intensity and the combination of the therapeutic parts still remain to be tested in order to achieve more satisfying effect. The result of combined use is as follows:",
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},
{
type: "word",
cont: "1). The Ability to Inhibit Phase Transformation",
class: "np5"
},
{
type: "word",
cont: "In order to verify the ability of the combination treatment of four therapeutic parts to inhibit phase transformation, we mixed the four therapeutic parts and conducted the experiment of inhibiting phase transformation. The results indicated that when the four therapeutic parts were combined, the number of hypha phase cells in the visual area decreased compared with that of the BDSF part alone, indicating that the mixture’s ability to inhibit hypha formation was stronger. ",
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{
type: "word",
cont: "2). The Ability to Destabilize Hyphae ",
class: "np5"
},
{
type: "word",
cont: "In order to verify the ability of the combination treatment of the four therapeutic parts to destabilize hyphae, we mixed the four therapeutic parts and conducted the experiment of destabilizing hyphae. The results indicated that when the four therapeutic parts were combined, the length of cells’ hypha in the visual area was shorter than that of the single part alone, indicating that the mixture’s ability to destabilize hyphae was stronger.",
class: "np5"
}, {
type: "pic",
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cont: [{
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},
{
type: "word",
cont: "3). The Ability to Kill (ita)C. albicans(itaed) ",
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},
{
type: "word",
cont: "To attest the ability of killing (ita)C. albicans(itaed) by the combination treatment of four therapeutic parts, we mixed the four therapeutic parts and tested the killing effect. The results indicated that cells were more sensitive to the mixed supernatant of four therapeutic parts, indicating that the mixture’s effect of killing (ita)C. albicans(itaed) is much better.",
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},
{
type: "pic",
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cont: [{
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]
},{ title: "Suicide Effect of Project", para: [
{ type: "word", cont: "Additionally, we tested the suicide system. We added 50 nM anhydrous tetracycline to the culture medium when the OD600 reached 0.9. After 24 hrs cultivation, the sharply decreased value of OD600 shows that the single suicide system could work successfully as our anticipation.", class: "np5" },
{ type: "word", cont: "This inspiring experiment result laid a solid foundation for the construct and identification of sensing-suicide system. <a href=\"https://2019.igem.org/Team:Jilin_China/Result\">(result)</a>", class: "np5" },
]
}, { title: "Sensing Effect of Project ", para: [
{ type: "word", cont: "To test the sensing system, we detected and documented the augment of fluorescence intensity at the 18th hour. Unfortunately, the result was less than satisfactory. Then, we consulted several professors with rich experience and made great efforts. However, we could not repeat the experiment for many time because of the limited time. But here’s the thing, the construct and identification of sensing system have been proved by reference, which had manifested such system could sense (ita)C. albicans(itaed) in engineered bacteria.", class: "np5" },
]
}, { title: "Conclusion", para: [
{ type: "word", cont: "The above experiments show that the function of the project has been verified. If time permits, we will continue to validate the composite components to test our system. Our system has reduced drug resistance and relapse compared to traditional methods, so the project has promising application value. Because of the novelty of our approach and the therapeutic potential we envision, we are very proud of what we have been able to achieve so far and are optimistic about the practical potential of our project.", class: "np5" },
]
}
]
}