Team:SUIS Shanghai/Basic Part

SUIS_Shanghai 2019 Basic Parts


Basic Parts Table

This year our teams created five new basic parts ouyr of our toital collection of 21. We feel our best part is BBa_K3031015 which is a variant of the luxI promoter regulatory sequence from the commonly used Quorum sensing bacterial system. We added a Ferric Uptake Regulator (FUR) sequence into this promoter meaning that when this is used as intended in the LuxR.LuxI system, the FUR sequence can inhibit the regulation of genes under the lux promoter in low iron concentrations. Please visit the part page for more deatails on its Biology and Usage as well as experiments used to characterize and validate this part.

We also created a new part (a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031018">BBa_K3031018) which is a mutated sequence of a previously existing transcription factor part which is activated when in the presence of Reactive Oxygen Species (ROS). This part was also experimentally validated and characterized on its part page on the registry. The other basic parts are coding regions associated specifically with our project and have applications in the development of live bacterial vaccine delivery systems.


Part Name Part Description Length
BBa_K3031015 LuxI promoter with FUR box located in the -10 region 166
BBa_K3031018 OxyR mutated at residues 147 & 203 915
BBa_K3031000 ORF81 - Membrane envelpe protein of Cyprinid Herpesvirus 3 959
BBa_K3031001 CwA - Cell wall anchoring whole protein from Lactobacillus plantarum 2118
BBa_K3031002 CwA (194aa) - Truncated cell wall anchoring protein from Lactobacillus plantarum truncated sequence 678