Team:Georgia State/Protocols

GSU iGEM

Protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli

    • Coral reef facts:

  • Provide $11.9 trillion per year
  • Support 500 million people worldwide
  • Over 50% of living coral has been lost
  • 25% of all marine species are at risk

    • Possible solutions:

  • Antioxidant enzyme superoxide dismutase
  • Heat shock proteins
  • Stress proteins hsp60 and hsp70
  • Fluorescent proteins
  • Mycosporine-like amino acids...natural sunscreens
  • More lipids or low molecular weight lipophilic compounds
  • More membrane stability = less ROS

    • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media

    • Other Techniques

  • Restriction Digest/Heat Kill
  • PCR
  • Overnight Cultures and Glycerol Stocks
  • Plasmid Extraction Mini Prep
  • Plasmid Extraction Midi Prep
  • Ligation
  • Gel Extraction
  • Gel Electrophoresis
  • Ethanol Precipitate
  • Lab Prep/Recipes

    • Transforming pCB302-gfp-MBD into E. coli

    Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP

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