Team:Georgia State/Protocols
put in the flow charts and create links to pdfs of our protocols
Transformation Protocols
Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
Symbiodinium microadriaticum Electroporation Transformations
Transforming A. tumefaciens
Transforming E.coli
ASP-8A Media
F2 Media
L1 Media
Restriction Digest/Heat Kill
PCR
Overnight Cultures and Glycerol Stocks
Plasmid Extraction Mini Prep
Plasmid Extraction Midi Prep
Ligation
Gel Extraction
Gel Electrophoresis
Ethanol Precipitate
Lab Prep/Recipes
Culturing Medias
Other Techniques
Transforming pCB302-gfp-MBD into E. coli
Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens
Codon Optimized RFP
Dino GFP
Dino RFP