Team:Georgia State/Protocols

GSU iGEM

Protocols

put in the flow charts and create links to pdfs of our protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli

    • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media

    • Other Techniques

  • Restriction Digest/Heat Kill
  • PCR
  • Overnight Cultures and Glycerol Stocks
  • Plasmid Extraction Mini Prep
  • Plasmid Extraction Midi Prep
  • Ligation
  • Gel Extraction
  • Gel Electrophoresis
  • Ethanol Precipitate
  • Lab Prep/Recipes

    • Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP

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    Contact Us

    Tel: (+1) 404 413 5344

    E-mail: igemgsu@gmail.com

    Address: iGEM 50 Decatur St SE, Atlanta GA 30303