Team:Georgia State/Protocols

GSU iGEM

Protocols

put in the flow charts and create links to pdfs of our protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli

    • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media

    • Restriction Digest/Heat Kill

    PCR

    Overnight Cultures and Glycerol Stocks

    Plasmid Extraction Mini Prep

    Plasmid Extraction Midi Prep

    Ligation

    Gel Extraction

    Gel Electrophoresis

    Ethanol Precipitate

    Lab Prep/Recipes

    Transforming pCB302-gfp-MBD into Escherichia coli

    Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP

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    Contact Us

    Tel: (+1) 404 413 5344

    E-mail: igemgsu@gmail.com

    Address: iGEM 50 Decatur St SE, Atlanta GA 30303