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JUDGING FORM ⇗

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Overview

“Human Practice is the study of how your work affects the world, and how the work affects your work.”--Peter Carr, Director of Judging.

Our main achievements of integrated human practices were to get valuable feedback for our product improvement, as well as to raise the public awareness on the issue of indoor air quality. To upgrade our prototype, we discussed with experts in different fields in algae culturing, protocol design, and product application, etc. During the discussion, we received a lot of practical and useful advices from these experts, and we further integrated those into our project and made CAir^TM as our outstanding product to meet customers’ expectations.

Motivation & Brainstorming

Measurements of CO2

We measured the air quality in many places with a sensor, Air Mentor. At the beginning, we took the sensor outdoor into those places, which the sensor was measuring constantly. Thus, we could see the data of all places are begin at the ppm around 400 to 700. Except for the gym, there are no people in those places at first, and then people flocked into rapidly. The following is the details and photos of those measurements:

1. Classroom: 40 people in one class, second class of senior second grade in Mingdao.
2. Outdoor: On the fence outside the biolab in MingDao.
3. TEDex Hall: 85 people in the TEDex Hall in MingDao.
4. SUV: 3 people in Mazda CX5 with closed circulation.
5. School Bus: 40 people in one school bus.
6. Table Tennis Room: 42 people in one table tennis room in Ming Hung department B1.
7. Gym: 30 to 40 people in civil sport center gym in Changhua county.

Factor 2: Modeling the Factors that Reduce Carbon Dioxide

The CO2 fixation process can be illustrated with the following diagram.

Therefore, the process of defining the sequestration of CO2 in our model can be divided into two aspects: determining the natural dissolution rate of CO2 in water (when CA is absent) and determining the increased dissolution rate of CO2 in water (when CA is present). However, the dissolved inorganic carbon (DIC; which implies the combination of H₂CO₃, HCO₃^-, _CO3^2-, and all other forms of dissolved carbon dioxide) in water can eventually reach the maximum capacity, which is itself dependent on the pH value of the environment. How rapidly this theoretical maximum is attained is dictated by the photosynthetic rate of cyanobacteria.

Factor 2a: Determining the Natural Dissolution Rate of CO2 in Water

The natural dissolution rate of CO2 in water (v_natural) is calculated using the first-order rate constant equation, as shown in Equation 3, where 0.039 s^-1 is the rate constant for the forward reaction of the hydration of carbon dioxide (CO₂+H₂O->H₂CO₃). The correlation between the concentration of gaseous carbon dioxide and the natural dissolution rate is illustrated in Figure 1.

Equation 3. First-order rate constant equation for hydration of carbon dioxide.

Figure 1. The linear curve modeling the correlation between the concentration of gaseous carbon dioxide and its natural dissolution rate in water.

Factor 2b: Determining the Enzymatic Reaction Rate of CA

We modeled the enzymatic rate of carbonic anhydrase with Michaelis-Menten kinetics, as shown in Equation 4. v_CA represents the enzymatic reaction rate of CA; [CO_2(g)] represents the concentration of substrate (in this case, gaseous carbon dioxide); v_max represents the maximum reaction rate possible; and K_m is the Michaelis-Menten constant, which is defined as the substrate concentration when the enzymatic reaction rate is half of its maximum (whenv=1/2v_max).

Equation 4. The Michaelis-Menten equation, which models the relationship between the concentration of gaseous carbon dioxide and the enzymatic reaction rate.

Equation 5. Calculation for v_max, the maximum reaction rate. K_cat denotes the catalyst rate constant of CA and [CA] represents the concentration of the enzyme.

Therefore, by inserting the v_max and K_m values into the model, the correlation between CO2 concentration and the reaction rate of CA can be shown. v_max can be calculated with Equation 5.
We obtained the K_cat and K_m values for the human carbonic anhydrase II (hCAII) from literature. According to Silverman et al., the K_cat and K_m values for hCAII are 1.4×10⁶ s^-1 and 9.3×10^-3 M, respectively. A [CA] value of 2.883×10^-3 M is used based on the enzyme we use. Unit conversions are shown below.

Using the enzymatic coefficients for the Michaelis-Menten model, we plotted the curve in Figure 2 to model the enzyme kinetics of human carbonic anhydrase II.

Figure 2. The Michaelis-Menten curve modeling the enzyme activities of hCAII at various concentrations of carbon dioxide.

Factor 2c: Determining the Photosynthetic Rate of Cyanobacteria in the Form of CO2 Consumption

After carbon dioxide has fully hydrated and dissolved into water, the last phase in our process of CO2 sequestration is its removal by the photosynthesis of cyanobacteria Synechococcus elongatus PCC7942. The rate at which the dissolved CO2 is consumed by our cyanobacteria can be modeled with Equation 5, which models the relationship between the concentration of dissolved inorganic carbon (DIC) and the rate of carbon-specific growth (Cμ; which is also equivalent to the rate of photosynthesis v_{photosynthesis}), which is represented in the form of O2 production (Clark). The conversion from the amount of O2 production to CO2 sequestration is then performed using the 1:1 molar ratio between O2 and CO2 obtained from the chemical equation of photosynthesis. The maximum rate of growth μ_max and the half saturation constant for C_μ, K_G(DIC), specific for the cyanobacteria Synechococcus are then consulted from literature. Calculations for the final function are shown below with the parameters listed in Table 3.

Equation 6. Relationship between the rate of photosynthesis (v_{photosynthesis}) and the concentration of dissolved inorganic carbon (DIC) (Clark).

Table 3. Parameters used in calculation for C_μ

Using the converted photosynthetic coefficients that we obtained through literature for our cyanobacteria Synechococcus elongatus, we constructed a graph in Figure 3 showing the relationship between the concentration of dissolved inorganic carbon in water and the rate of CO2 consumption due to photosynthesis.

Figure 3. The Michaelis-Menten curve modeling the photosynthetic activity of Synechococcus elongatus. The CO2 consumption rate at various concentrations of dissolved inorganic carbon (DIC) is shown.

Factor 2d: Determining the Solubility of CO2 in Alkaline Environment

Our Final Model

References