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<p>Nowadays the problem of microplastics in fluids like drinkable water is a huge topic with a lot of new publications and studies about the amount, types and risks of it for animals, environment and humans. The `Plastractor` is a device which shall extract microplastics from fluids easily via magnets. Therefore the bacterium Rhodospirillum rubrum `magneticum` was obtained. It is genetically modified to build magnetosomes; small magnetic and vesicle-like particles, with plastic binding peptides on its membrane. For modification we use E. coli BW29427 which transfers the needed plasmid to Rhodospirillum rubrum `magneticum` via conjugation. Two different plastic binding peptides are used, `Tachystatin A2` (TA2) and `liquid chromatography peak I` (LCI), fused with two different fluorescent proteins to enable the detection of the bound particles. Thus the device will be able to extract the plastics that are bound to magnetosomes and detect it by fluorescence.</p> | <p>Nowadays the problem of microplastics in fluids like drinkable water is a huge topic with a lot of new publications and studies about the amount, types and risks of it for animals, environment and humans. The `Plastractor` is a device which shall extract microplastics from fluids easily via magnets. Therefore the bacterium Rhodospirillum rubrum `magneticum` was obtained. It is genetically modified to build magnetosomes; small magnetic and vesicle-like particles, with plastic binding peptides on its membrane. For modification we use E. coli BW29427 which transfers the needed plasmid to Rhodospirillum rubrum `magneticum` via conjugation. Two different plastic binding peptides are used, `Tachystatin A2` (TA2) and `liquid chromatography peak I` (LCI), fused with two different fluorescent proteins to enable the detection of the bound particles. Thus the device will be able to extract the plastics that are bound to magnetosomes and detect it by fluorescence.</p> | ||
</div> | </div> |
Revision as of 20:41, 19 November 2019
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Aachen
<svg enable-background="new 0 0 250 250" version="1.1" viewBox="0 0 250 250" xml:space="preserve" height="20px"> <style type="text/css"> .st0{fill:#085156; margin-bottom:-5px} </style> <circle class="st0" cx="125" cy="104.1" r="17.9"/> <path class="st0" d="m125 33c-50.8 0-92 41.2-92 92s41.2 92 92 92 92-41.2 92-92-41.2-92-92-92zm31.6 98l-31.6 53.7-31.6-53.7c-3.7-6.4-7.2-17.3-7.2-25.4 0-22.2 17.4-40.3 38.8-40.3s38.8 18 38.8 40.3c0 8-3.5 19-7.2 25.4z"/></svg> Institution: Aachen University
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Location: Germany
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Region: Europe
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<path class="st0" d="m125 33c-50.8 0-92 41.2-92 92s41.2 92 92 92 92-41.2 92-92-41.2-92-92-92zm31.6 98l-31.6 53.7-31.6-53.7c-3.7-6.4-7.2-17.3-7.2-25.4 0-22.2 17.4-40.3 38.8-40.3s38.8 18 38.8 40.3c0 8-3.5 19-7.2 25.4z"/></svg>
Section: Overgrad
<svg enable-background="new 0 0 250 250" version="1.1" viewBox="0 0 250 250" xml:space="preserve" height="20px"> <style type="text/css"> .st0{fill:#085156; margin-bottom:-5px} </style> <circle class="st0" cx="125" cy="104.1" r="17.9"/> <path class="st0" d="m125 33c-50.8 0-92 41.2-92 92s41.2 92 92 92 92-41.2 92-92-41.2-92-92-92zm31.6 98l-31.6 53.7-31.6-53.7c-3.7-6.4-7.2-17.3-7.2-25.4 0-22.2 17.4-40.3 38.8-40.3s38.8 18 38.8 40.3c0 8-3.5 19-7.2 25.4z"/></svg> Track: Environment
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Medal: Gold
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Nominations: Best Environment Project
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Awards: -
Plastractor - extracting microplastics from fluids via magnets
<svg enable-background="new 0 0 250 250" version="1.1" viewBox="0 0 250 250" xml:space="preserve" height="20px"> <style type="text/css"> .st0{fill:#085156; margin-bottom:-5px} </style> <circle class="st0" cx="125" cy="104.1" r="17.9"/> <path class="st0" d="m125 33c-50.8 0-92 41.2-92 92s41.2 92 92 92 92-41.2 92-92-41.2-92-92-92zm31.6 98l-31.6 53.7-31.6-53.7c-3.7-6.4-7.2-17.3-7.2-25.4 0-22.2 17.4-40.3 38.8-40.3s38.8 18 38.8 40.3c0 8-3.5 19-7.2 25.4z"/></svg> Parts: <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=Aachen">parts/Team:Aachen</a>
<svg enable-background="new 0 0 250 250" version="1.1" viewBox="0 0 250 250" xml:space="preserve" height="20px"> <style type="text/css"> .st0{fill:#085156; margin-bottom:-5px} </style> <circle class="st0" cx="125" cy="104.1" r="17.9"/> <path class="st0" d="m125 33c-50.8 0-92 41.2-92 92s41.2 92 92 92 92-41.2 92-92-41.2-92-92-92zm31.6 98l-31.6 53.7-31.6-53.7c-3.7-6.4-7.2-17.3-7.2-25.4 0-22.2 17.4-40.3 38.8-40.3s38.8 18 38.8 40.3c0 8-3.5 19-7.2 25.4z"/></svg> Wiki: <a href="https://2019.igem.org/Team:Aachen">2019/Team:Aachen</a>
Nowadays the problem of microplastics in fluids like drinkable water is a huge topic with a lot of new publications and studies about the amount, types and risks of it for animals, environment and humans. The `Plastractor` is a device which shall extract microplastics from fluids easily via magnets. Therefore the bacterium Rhodospirillum rubrum `magneticum` was obtained. It is genetically modified to build magnetosomes; small magnetic and vesicle-like particles, with plastic binding peptides on its membrane. For modification we use E. coli BW29427 which transfers the needed plasmid to Rhodospirillum rubrum `magneticum` via conjugation. Two different plastic binding peptides are used, `Tachystatin A2` (TA2) and `liquid chromatography peak I` (LCI), fused with two different fluorescent proteins to enable the detection of the bound particles. Thus the device will be able to extract the plastics that are bound to magnetosomes and detect it by fluorescence.