Difference between revisions of "Team:Georgia State/AlgaeObservations"

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                 <td id="date"><a href="070119.pdf"><span class="tool" data-tip="Half of S. Microadriaticum were alive and half were dead, most of the D. Tertiolecta were swimming around and some were shaking in place, and most of the O. Marina were swimming freely. Click to see more.">1</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/f/fe/T--Georgia_State--culturing_070119.pdf"><span class="tool" data-tip="Half of S. Microadriaticum were alive and half were dead, most of the D. Tertiolecta were swimming around and some were shaking in place, and most of the O. Marina were swimming freely. Click to see more.">1</span></td>
                 <td id="date"><a href="070219.pdf"><span class="tool" data-tip="The cells in the O. Marina original stock, O. Marina ASP-8A 75mL stock, O. Marina ASP-8A 25 mL stock, and D. Tertiolecta ASP-8A 9 mL were actively swimming. The cells of S. Microadriaticum original stock were actively shaking. Click to see more.">2</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/b/bc/T--Georgia_State--culturing_070219.pdf"><span class="tool" data-tip="The cells in the O. Marina original stock, O. Marina ASP-8A 75mL stock, O. Marina ASP-8A 25 mL stock, and D. Tertiolecta ASP-8A 9 mL were actively swimming. The cells of S. Microadriaticum original stock were actively shaking. Click to see more.">2</span></td>
                 <td id="date"><a href="070319.pdf"><span class="tool" data-tip="Majority of cells of S. Microadriaticum were swimming or spinning but a few of them were dead, majority of the cells of D. Tertiolecta were swimming or shaking in place, and the cells of O. Marina showed normal activity. Click to see more.">3</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/2/26/T--Georgia_State--culturing_070319.pdf"><span class="tool" data-tip="Majority of cells of S. Microadriaticum were swimming or spinning but a few of them were dead, majority of the cells of D. Tertiolecta were swimming or shaking in place, and the cells of O. Marina showed normal activity. Click to see more.">3</span></td>
                 <td id="date"><a href="070419.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles whiles ¼ of the cells are not moving, majority of the cells of D. Tertiolecta showed normal activity while ⅓ of the cells were shaking in place, and  the cells of  O. Marina showed normal activity but were slightly green. Click to see more.">4</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/f/f2/T--Georgia_State--culturing_070419.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles whiles ¼ of the cells are not moving, majority of the cells of D. Tertiolecta showed normal activity while ⅓ of the cells were shaking in place, and  the cells of  O. Marina showed normal activity but were slightly green. Click to see more.">4</span></td>
                 <td id="date"><a href="070519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles, but a few were dead or swimming around, ¼ of the cells of D. Tertiolecta were shaking, ¼ of the cells were were showing normal activity, and the rest of the cells were dead, and all the cells of O. Marina showed normal activity. Click to see more.">5</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/c/c3/T--Georgia_State--culturing_070519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles, but a few were dead or swimming around, ¼ of the cells of D. Tertiolecta were shaking, ¼ of the cells were were showing normal activity, and the rest of the cells were dead, and all the cells of O. Marina showed normal activity. Click to see more.">5</span></td>
 
                 <td id="date-pdf">6</td>
 
                 <td id="date-pdf">6</td>
 
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                 <td id="date-pdf">7</td>
 
                 <td id="date-pdf">7</td>
                 <td id="date"><a href="070819.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning, half of the cells of D. Tertiolecta were shaking in place and the other half were swimming, and the cells of O. Marina were swimming fast. Click to see more.">8</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/6/65/T--Georgia_State--culturing_070819.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning, half of the cells of D. Tertiolecta were shaking in place and the other half were swimming, and the cells of O. Marina were swimming fast. Click to see more.">8</span></td>
                 <td id="date-pdf">9</td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/b/b0/T--Georgia_State--culturing_070919.pdf"><span class="tool" data-tip="Click to see more.">9</td>
                 <td id="date"><a href="071019.pdf"><span class="tool" data-tip="All of the cells of S. Microadriaticum, D. Tertiolecta, and O. Marina showed normal activity. Click to see more.">10</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/5/5c/T--Georgia_State--culturing_071019.pdf"><span class="tool" data-tip="All of the cells of S. Microadriaticum, D. Tertiolecta, and O. Marina showed normal activity. Click to see more.">10</span></td>
                 <td id="date"><a href="071119.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles, few of them were spinning, but a couple of the cells were dead, majority of the cells of D. Tertiolecta were swimming, some of them were shaking in place, but a few were non-moving, and all of the cells of O. Marina were green and showed normal activity. Click to see more.">11</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/8/86/T--Georgia_State--culturing_071119.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were spinning in circles, few of them were spinning, but a couple of the cells were dead, majority of the cells of D. Tertiolecta were swimming, some of them were shaking in place, but a few were non-moving, and all of the cells of O. Marina were green and showed normal activity. Click to see more.">11</span></td>
                 <td id="date"><a href="071219.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were densely packed and rapidly swimming in circles, there was an overgrowth of the cells of D. Tertiolecta, many were swimming but there was a noticeable amount of cells that were immobile or dead. Click to see more.">12</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/b/b9/T--Georgia_State--culturing_071219.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were densely packed and rapidly swimming in circles, there was an overgrowth of the cells of D. Tertiolecta, many were swimming but there was a noticeable amount of cells that were immobile or dead. Click to see more.">12</span></td>
 
                 <td id="date-pdf">13</td>
 
                 <td id="date-pdf">13</td>
 
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                 <td id="date-pdf">14</td>
 
                 <td id="date-pdf">14</td>
                 <td id="date"><a href="071519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were dead and clumped together, but there were some cells that were swirling in circles, ⅓ of the cells of D. Tertiolecta were shaking in place and the rest showed normal activity, and the cells of swimming O. Marina have a conch shell shape while the immobile cells tend to be rounder. Click to see more.">15</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/c/c3/T--Georgia_State--culturing_071519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were dead and clumped together, but there were some cells that were swirling in circles, ⅓ of the cells of D. Tertiolecta were shaking in place and the rest showed normal activity, and the cells of swimming O. Marina have a conch shell shape while the immobile cells tend to be rounder. Click to see more.">15</span></td>
                 <td id="date"><a href="071619.pdf"><span class="tool" data-tip="Some of the cells of S. Microadriaticum were dead and clustered together, the other cells were shaking separately, ⅕ of the cells of D. Tertiolecta were shaking and ⅘ of the cells were swimming actively, and the cells of O. Marina looked purple but were swimming actively and rapidly. Click to see more.">16</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/f/fc/T--Georgia_State--culturing_071619.pdf"><span class="tool" data-tip="Some of the cells of S. Microadriaticum were dead and clustered together, the other cells were shaking separately, ⅕ of the cells of D. Tertiolecta were shaking and ⅘ of the cells were swimming actively, and the cells of O. Marina looked purple but were swimming actively and rapidly. Click to see more.">16</span></td>
                 <td id="date"><a href="071719.pdf"><span class="tool" data-tip="Only one part of the S. Microadriaticum’s cells were clustered and dead, the other cells circled actively, ⅕ of the cells of D. Tertiolecta were shaking and the rest showed normal activity, and the babies and large cells of O. Marina were swimming actively while one was spinning. Click to see more.">17</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/b/be/T--Georgia_State--culturing_071719.pdf"><span class="tool" data-tip="Only one part of the S. Microadriaticum’s cells were clustered and dead, the other cells circled actively, ⅕ of the cells of D. Tertiolecta were shaking and the rest showed normal activity, and the babies and large cells of O. Marina were swimming actively while one was spinning. Click to see more.">17</span></td>
                 <td id="date"><a href="071819.pdf"><span class="tool" data-tip="Most of the cells of S. Microadriaticum were non-moving, the cells that were clumped together looked greener than the other cells that had a brownish color, half of the cells of D. Tertiolecta were swimming and the other half were shaking, and the cells of O. Marina showed normal activity. Click to see more.">18</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/6/69/T--Georgia_State--culturing_071819.pdf"><span class="tool" data-tip="Most of the cells of S. Microadriaticum were non-moving, the cells that were clumped together looked greener than the other cells that had a brownish color, half of the cells of D. Tertiolecta were swimming and the other half were shaking, and the cells of O. Marina showed normal activity. Click to see more.">18</span></td>
 
                 <td id="date"><a href="071919.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were in place swimming in circles, majority of the cells of D. Tertiolecta showed normal activity, some cells were non-moving, and many on the outskirts of the coverslip were dead, and the cells of O. Marina showed were swimming actively. Click to see more.">19</span></td>
 
                 <td id="date"><a href="071919.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were in place swimming in circles, majority of the cells of D. Tertiolecta showed normal activity, some cells were non-moving, and many on the outskirts of the coverslip were dead, and the cells of O. Marina showed were swimming actively. Click to see more.">19</span></td>
 
                 <td id="date-pdf">20</td>
 
                 <td id="date-pdf">20</td>
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                 <td id="date-pdf">21</td>
 
                 <td id="date-pdf">21</td>
                 <td id="date"><a href="072219.pdf"><span class="tool" data-tip="⅔ of the cells of S. Microadriaticum were swimming and the rest of the cells were circling. Click to see more.">22</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/2/20/T--Georgia_State--culturing_072219.pdf"><span class="tool" data-tip="⅔ of the cells of S. Microadriaticum were swimming and the rest of the cells were circling. Click to see more.">22</span></td>
                 <td id="date"><a href="072319.pdf"><span class="tool" data-tip="The cells of S. Microadriaticum were spinning actively, but no cells were found in the L1 + F2 75 mL stock, ⅔ of the cells of D. Tertiolecta were swimming and the rest were shaking, and the cells of O. Marina had a purple and green color but were swimming actively. Click to see more.">23</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/0/03/T--Georgia_State--culturing_072319.pdf"><span class="tool" data-tip="The cells of S. Microadriaticum were spinning actively, but no cells were found in the L1 + F2 75 mL stock, ⅔ of the cells of D. Tertiolecta were swimming and the rest were shaking, and the cells of O. Marina had a purple and green color but were swimming actively. Click to see more.">23</span></td>
                 <td id="date"><a href="072419.pdf"><span class="tool" data-tip="The cells of S. Microadriaticum were spinning, more cells were darker than others, ⅔ of the cells of D. Tertiolecta were swimming actively while the rest were shaking. Click to see more.">24</span></td>
+
                 <td id="date"><a href="https://static.igem.org/mediawiki/2019/5/54/T--Georgia_State--culturing_072419.pdf"><span class="tool" data-tip="The cells of S. Microadriaticum were spinning, more cells were darker than others, ⅔ of the cells of D. Tertiolecta were swimming actively while the rest were shaking. Click to see more.">24</span></td>
 
                 <td id="date"><a href="072519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were swimming but some were swimming in loops, some of the cells of D. Tertiolecta were shaking and some were swimming round, the cells of O. Marina were swimming but the cells in the ASP-8A 10 mL stock didn’t show an overgrowth and they seem to collect at the bottom of the tube. Click to see more.">25</span></td>
 
                 <td id="date"><a href="072519.pdf"><span class="tool" data-tip="Majority of the cells of S. Microadriaticum were swimming but some were swimming in loops, some of the cells of D. Tertiolecta were shaking and some were swimming round, the cells of O. Marina were swimming but the cells in the ASP-8A 10 mL stock didn’t show an overgrowth and they seem to collect at the bottom of the tube. Click to see more.">25</span></td>
 
                 <td id="date-pdf">26</td>
 
                 <td id="date-pdf">26</td>
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               <td id="date"><a href="081219"><span class="tool" data-tip="The light intensity and temperature was measured every 10 seconds for 1 minute and averaged. 10 uL of cells were loaded onto the hemacytometer. In the hood, the average light intensity was 74.78 and the temperature was 23.6°C.  In the window, the average light intensity was 90.91 and the temperature was  21.5°C. Click to see more.">12</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/3/35/T--Georgia_State--culturing_081219.pdf"><span class="tool" data-tip="The light intensity and temperature was measured every 10 seconds for 1 minute and averaged. 10 uL of cells were loaded onto the hemacytometer. In the hood, the average light intensity was 74.78 and the temperature was 23.6°C.  In the window, the average light intensity was 90.91 and the temperature was  21.5°C. Click to see more.">12</td>
 
               <td id="date-pdf">13</td>
 
               <td id="date-pdf">13</td>
               <td id="date"><a href="081419"><span class="tool" data-tip=" Under the hood, the average light intensity was 80.48 and the temperature was 23.2°C. In the window, the average light intensity was 89.77 and the temperature was 21.7°C. Click to see more.">14</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/5/57/T--Georgia_State--culturing_081419.pdf"><span class="tool" data-tip=" Under the hood, the average light intensity was 80.48 and the temperature was 23.2°C. In the window, the average light intensity was 89.77 and the temperature was 21.7°C. Click to see more.">14</td>
 
               <td id="date-pdf" >15</td>
 
               <td id="date-pdf" >15</td>
 
               <td id="date-pdf" >16</td>
 
               <td id="date-pdf" >16</td>
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               <td id="date"><a href="081919"><span class="tool" data-tip="Under the hood, S. Microadriaticum’s cells grew faster than the one near the window. For O. Marina, there was some D. Tertiolecta cells found swimming in the sample and they swim faster than the algae in filtered seawater. The average light intensity was 63.14 and the temperature was 26.1°C. In the window, most of S. Microadriaticum weren’t moving in both medias and a surprising amount seems to dividing. For O. Marina, the F/2 cells swim more actively and don’t appear dark. In filtered SW, O. Marina cells appear dark and round. Within 4 minute time span, the large cells burst open. The average light intensity was 92.34 and the temperature was 21.5°C. Click to see more.">19</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/d/dd/T--Georgia_State--culturing_081919.pdf"><span class="tool" data-tip="Under the hood, S. Microadriaticum’s cells grew faster than the one near the window. For O. Marina, there was some D. Tertiolecta cells found swimming in the sample and they swim faster than the algae in filtered seawater. The average light intensity was 63.14 and the temperature was 26.1°C. In the window, most of S. Microadriaticum weren’t moving in both medias and a surprising amount seems to dividing. For O. Marina, the F/2 cells swim more actively and don’t appear dark. In filtered SW, O. Marina cells appear dark and round. Within 4 minute time span, the large cells burst open. The average light intensity was 92.34 and the temperature was 21.5°C. Click to see more.">19</td>
 
               <td id="date-pdf">20</td>
 
               <td id="date-pdf">20</td>
               <td id="date-pdf"> <a href="082119"><span class="tool" data-tip="Under the hood, S. Microadriaticum cells were not moving and the O. Marina were less active. The average light intensity was 65.01 and the temperature was 23.2°C. In the window, half of S. Microadriaticum cells were moving and the other half was not moving and there were unknown yellow residue found in F/2 medium with the O. Marina cells. The average light intensity was 58.39 and the temperature was 21.1°C. Click to see more.">21</td>
+
               <td id="date-pdf"> <a href="https://static.igem.org/mediawiki/2019/1/1c/T--Georgia_State--culturing_082119.pdf"><span class="tool" data-tip="Under the hood, S. Microadriaticum cells were not moving and the O. Marina were less active. The average light intensity was 65.01 and the temperature was 23.2°C. In the window, half of S. Microadriaticum cells were moving and the other half was not moving and there were unknown yellow residue found in F/2 medium with the O. Marina cells. The average light intensity was 58.39 and the temperature was 21.1°C. Click to see more.">21</td>
               <td id="date"><a href="082219"><span class="tool" data-tip="Under the hood, half of the O. Marina cells were staggering while the other half were not moving. The light intensity was 87.42 and the temperature was 23.9°C. In the window, ⅓ of the S. Microadriaticum cells were moving and majority of the O. Marina cells were moving. The light intensity was 69.93 and the temperature was 22.3°C. Click to see more.">22</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/e/e0/T--Georgia_State--culturing_082219.pdf"><span class="tool" data-tip="Under the hood, half of the O. Marina cells were staggering while the other half were not moving. The light intensity was 87.42 and the temperature was 23.9°C. In the window, ⅓ of the S. Microadriaticum cells were moving and majority of the O. Marina cells were moving. The light intensity was 69.93 and the temperature was 22.3°C. Click to see more.">22</td>
               <td id="date"><a href="082319"><span class="tool" data-tip="Under the hood, majority of the S. Microadriaticum cells were in clumps and not moving. The average light intensity was 66.92 and the temperature was 23.2°C. In the window, the average light intensity was 41.42 and the temperature was 20.8°C. Click to see more.">23</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/6/68/T--Georgia_State--culturing_082319.pdf"><span class="tool" data-tip="Under the hood, majority of the S. Microadriaticum cells were in clumps and not moving. The average light intensity was 66.92 and the temperature was 23.2°C. In the window, the average light intensity was 41.42 and the temperature was 20.8°C. Click to see more.">23</td>
 
               <td id="date-pdf">24</td>
 
               <td id="date-pdf">24</td>
 
               <td id="date-pdf">25</td>
 
               <td id="date-pdf">25</td>
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               <td id="date"><a href="082319"><span class="tool" data-tip="Under the hood, majority of S. Microadriaticum cells were moving in the ASP-8A media. The lights were unplugged at 6:30pm. The average light intensity was unknown and the temperature was 23.2°C. In the window, the average light intensity was unknown and the temperature was 20.8°C. Click to see more.">26</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/e/ee/T--Georgia_State--culturing_082619.pdf"><span class="tool" data-tip="Under the hood, majority of S. Microadriaticum cells were moving in the ASP-8A media. The lights were unplugged at 6:30pm. The average light intensity was unknown and the temperature was 23.2°C. In the window, the average light intensity was unknown and the temperature was 20.8°C. Click to see more.">26</td>
 
               <td id="date-pdf">27</td>
 
               <td id="date-pdf">27</td>
 
               <td id="date-pdf">28</td>
 
               <td id="date-pdf">28</td>
               <td id="date"><a href="082919"><span class="tool" data-tip="Photometer was dead and batteries have not been changed. Under the hood, the average light intensity was unknown and the temperature was 26.3°C. In the window, the average light intensity was unknown and the temperature was 21.2°C. Click to see more.">29</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/3/3e/T--Georgia_State--culturing_082919.pdf"><span class="tool" data-tip="Photometer was dead and batteries have not been changed. Under the hood, the average light intensity was unknown and the temperature was 26.3°C. In the window, the average light intensity was unknown and the temperature was 21.2°C. Click to see more.">29</td>
               <td id="date"><a href="083019"><span class="tool" data-tip="Under the hood, very few S. Microadriaticum cells were moving. The average light intensity was unknown and the temperature was unknown. In the window, the average light intensity was unknown and the temperature was 20.4°C. Click to see more.">30</td>
+
               <td id="date"><a href="https://static.igem.org/mediawiki/2019/a/a7/T--Georgia_State--culturing_083019.pdf"><span class="tool" data-tip="Under the hood, very few S. Microadriaticum cells were moving. The average light intensity was unknown and the temperature was unknown. In the window, the average light intensity was unknown and the temperature was 20.4°C. Click to see more.">30</td>
 
               <td id="date-pdf">31</td>
 
               <td id="date-pdf">31</td>
 
               <td id="date-pdf"></td>
 
               <td id="date-pdf"></td>

Revision as of 22:43, 21 October 2019

Algae Observations

ALGAE LOG

Culturing during July & August
July 2019
S M T W T F S
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August 2019
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Videos 1 and 2


A concentration of 1,092,000 Symbiodinium cells per mL. This is a reference of a concentration cell count viewed under 4X and 10X magnification. Click the pictures above to see the videos!

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Videos 3 and 4


Predator-Prey culture of O. marina fed 2.0 mL of D. tertiolecta. The O. marina culture that was fed 2.0 mL of D. tertiolecta was not fed since 9/18/19. The video depicts the concentration of in the Dunaliella vs. Oxyrrhis as of 10/18/19.

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The More You Know


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