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| − | <h1>Optimization Of Environmentally Friendly Indigo Dye Production Method By Using Genetically Modified Bacteria</h1> | + | <script src="https://2019.igem.org/wiki/index.php?title=Team:Tongji_China/tags/v0.2/js/description.js&action=raw&ctype=text/javascript"></script> |
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| − | <h1>Project Inspiration and Description </h1>
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| − | <h2>Project Inspiration</h2>
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| − | <p>The representative blue of denim fabrics usually derives from indigo, and the high demand for such dyes has led to the production of indigo by chemical synthesis on an industrial scale. A variety of toxic chemicals are used in this process, and the degree of environmental pollution is particularly severe. Inadvertent use may also cause serious safety accidents.</p>
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| − | </div>
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| − | <h2>Project Description</h2>
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| − | <h3>Foundation</h3>
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| − | <p>The team at the University of California, Berkeley, has tried to use genetically engineered bacteria to produce a related compound: indoxyl. Indoxyl itself is easily oxidized, and its stability can be enhanced by glycosylation. When it is to be dyed, it can be deglycosylated to obtain indoxyl which can spontaneously oxidize to form indigo.</p>
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| − | <p>However, this pathway is not perfect. Carbon source without glucose must be used in the production process. Meanwhile, it faces various problems such as low product concentration, which leads to a high cost and make it difficult to be applied in actual production.</p>
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| − | <h3>Our Goals</h3>
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| − | <p>This year, we will work on lowering the cost of this pathway.</p>
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| − | <p>To promote the practical application of this method, we plan to remove the inhibition coming from glucose to the circuit based on team Berkeley 2013 to make the use of low-cost carbon sources possible and try to find a cost-effective indole donor and with the research of related industries, designing a accessible environmentally friendly indigo dye production system with application value.</p>
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| − | <p>Besides, we plan to characterize the influence of upregulating NADPH level of chassis, which we believe it will higher the efficiency of indican producing.</p>
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| − | <h1>Project Inspiration and Description </h1>
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| − | <h3>NEW: Bronze Medal Criterion #4</h3>
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| − | <p>Document how and why you chose your iGEM project on this page. Reference work outside or inside of iGEM that inspired your project, how you selected your project goal, and why you thought this project was a useful application of synthetic biology. Finally, provide a clear and concise description of what you plan on doing for your project.</p>
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| − | <p>To be eligible for this award, you must add clear documentation to this page and delete the alert box at the top of this page.</p>
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| − | <h3>What should this page contain?</h3>
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| − | <ul>
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| − | <li> A clear and concise description of your project.</li>
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| − | <li>A detailed explanation of why your team chose to work on this particular project.</li>
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| − | <li>References and sources to document your research.</li>
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| − | <li>Use illustrations and other visual resources to explain your project.</li>
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| − | <h3>Inspiration</h3>
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| − | <p>See how other teams have described and presented their projects: </p>
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| − | <ul>
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| − | <li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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| − | <li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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| − | <li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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| − | <li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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| − | </ul>
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| − | </div>
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| − | <h3>Advice on writing your Project Description</h3>
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| − | <p>
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| − | We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
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| − | </p>
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| − | <h3>References</h3>
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| − | <p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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