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| − | </head>
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| − | <body>
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| − | <div class="bgd" id="head">
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| − | <div class="contents" style="color: white">
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| − | <div class="column">
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| − | <div class="centers">
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| − | <div class="fb_72">
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| − | <b>Collaboration</b>
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| − | </div>
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| − | <div class="split"></div>
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| − | <div class="contents" id="phage">
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| − | <div class="column">
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| − | <div class="centers">
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| − | <div class="fb_48">
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| − | <b>OUC-China</b>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2019/5/55/T--JiangnanU_China--collaborations_1.png"
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| − | style="width: 30%;height: 30%;">
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| − | <img src="https://static.igem.org/mediawiki/2019/6/65/T--JiangnanU_China--collaboration_12.png"
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| − | style="width: 20%;height: 20%;margin: 10%">
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| − | <img src="https://static.igem.org/mediawiki/2019/b/ba/T--JiangnanU_China--collaborations_2.png"
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <div class="fm_22">
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| − | Phage infection occurs accidentally not only in the fermentation factories but also in the lab, and it
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| − | might get worse and worse without appropriate treatment. Therefore, we’d like to dedicate a booklet
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| − | about prevention, identification, and control of bacteriophage infection. By coincidence, we met team
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| − | OUC-China in the CCiC whose strain died abnormally in the shaking flask. Having heard our anti-phage
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| − | project, they wanted to figure out whether they encountered a phage infection. We sent the script of our
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| − | booklet to them, and they easily checked that the death strain was not caused by phage. Meanwhile, with
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| − | the feedback of their identification, we together improved the PICP (Prevention, Identification, Control
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| − | of Phage) booklet（链接）, allowing more teams to maintain a phage-free lab environment.
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| − | </div>
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| − | <!-- 复旦-->
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| − | <div class="fb_48">
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| − | <b>Fudan</b>
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| − | </div>
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| − | <div class="row" style="justify-items: center;align-content: center">
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| − | <img src="https://static.igem.org/mediawiki/2019/3/3b/T--JiangnanU_China--collaborations_0.png"
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| − | style="width: 33%;height: 33%;">
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| − | <img src="https://static.igem.org/mediawiki/2019/6/65/T--JiangnanU_China--collaboration_12.png"
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| − | style="width: 20%;height: 20%;margin: 10%">
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| − | <img src="https://static.igem.org/mediawiki/2019/b/ba/T--JiangnanU_China--collaborations_2.png"
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| − | style="width: 33%;height: 33%;">
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <div class="fm_22">
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| − | Our team achieved an anti-phage strategy against infection in different infection periods. Therefore,
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| − | it’s essential to control the promoter strength for the potential leakage or inclusion body problems.
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| − | Fortunately, team Fudan inspired us that it might be interesting to use ANN for promoter strength
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| − | prediction, which will save us a lot of time for test hundreds of promoters one by one in experiment.
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| − | Then, they gave us a paper1 about the similar prediction method, allowing us to establish an ANN
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| − | promoter strength prediction model of our own. With their help we can select more suitable promoters
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| − | designed by computer simulation.
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <img src="https://static.igem.org/mediawiki/2019/7/70/T--JiangnanU_China--collaborations_5.png"
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| − | style="width: 100%;height: auto;">
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| − | <div class="split_small"></div>
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| − | <img src="https://static.igem.org/mediawiki/2019/3/3d/T--JiangnanU_China--collaborations_6.png"
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| − | style="width: 100%;height: auto;">
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| − | <div class="split_small"></div></div>
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| − | <div class="fb_48">
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| − | <b>Jilin</b>
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <div class="row">
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| − | <img src="https://static.igem.org/mediawiki/2019/b/bc/T--JiangnanU_China--collaborations_3.png"
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| − | style="width: 33%;height: 33%;">
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| − | <img src="https://static.igem.org/mediawiki/2019/6/65/T--JiangnanU_China--collaboration_12.png"
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| − | style="width: 20%;height: 20%;margin: 10%">
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| − | <img src="https://static.igem.org/mediawiki/2019/b/ba/T--JiangnanU_China--collaborations_2.png"
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| − | style="width: 33%;height: 33%;">
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <div class="fm_22">
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| − | 1. measurement
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| − | Team Jilin_China has measured the relative promoter strength of the P59 to Anderson promoter family,
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| − | however the measurement results based on fluorescence different due to various experiment conditions.
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| − | Therefore, they’d like to know whether they could repeat the experiment and get the same result in
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| − | different labs. So they invited us to measure the 6 parts in our lab.
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| − | Jilin_China have sent BBa_J23119、BBa_J23104、BBa_J23108、BBa_J23105、BBa_J23114 and P59 promoters to us
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| − | for measurement, and the results are similar at 485 nm (excitation) / 528 nm (emission).
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| − | Click <a href="https://2019.igem.org/Team:Jilin_China/Collaborations">here</a>for more Jilin_China measurement
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| − | data.
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <img src="https://static.igem.org/mediawiki/2019/e/eb/T--JiangnanU_China--jilin.png"
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| − | style="width: 100%;height: auto;">
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| − | <div class="split_small"></div>
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| − | <div class="fm_18">
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| − | Figure 1. Relative fluorescence (fluorescence/OD(sub)600(subed)) intensity at emission wavelength 528 nm
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| − | under xcitation wavelength 485 nm of each promoter measured by (A) Jilin_China and (B) JiangnanU_China over time.</div><br/>
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| − | <div class="fm_22">
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| − | We aimed to construct a phage-resistant <i>E. coli</i> strain which can be applied to industrial
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| − | fermentation production in the future, so we needed to verify experiment under the same protocol condition
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| − | in different laboratories, thus we invited Jilin_China to help to test whether <i>E. coli</i> would use a
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| − | large amount of energy to express anti-phage parts, which greatly affected their own growth. The curve of <i>E. coli</i> growth they made was applied to our model to select the anti-phage part that had the
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| − | least impact on bacterial growth.
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| − | </div>
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| − | <div class="split_small"></div>
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| − | <img src="https://static.igem.org/mediawiki/2019/e/ee/T--JiangnanU_China--team_collaboration_12.png"
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| − | style="width: 100%;height: auto;">
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2019/a/a5/T--JiangnanU_China--footer_1.png"
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