Difference between revisions of "Team:Georgia State/Protocols"

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                        <h5>Coral reef facts:</h5>
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                    <h5>Culturing Medias</h5>
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                        <p><li>Provide $11.9 trillion per year</li>  
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<p><li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a></li>
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<li>Support 500 million people worldwide</li>
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<li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li>
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<li>Over 50% of living coral has been lost</li>
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li> </p>
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<li>25% of all marine species are at risk</li></p>
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                        <h5>Possible solutions:</h5>
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<h5>Other Techniques</h5>
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                        <p><li>Antioxidant enzyme superoxide dismutase</li>
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<li>Heat shock proteins</li>
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<li>Stress proteins hsp60 and hsp70</li>
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<li>Fluorescent proteins</li>
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<li>Mycosporine-like amino acids...natural sunscreens</li>
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<li>More lipids or low molecular weight lipophilic compounds</li>
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<li>More  membrane stability = less ROS</li></p>
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<h4>Culturing Medias</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li>
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<h4>Other Techniques</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li>
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Revision as of 01:40, 21 October 2019

GSU iGEM

Protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli

    • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media

    • Other Techniques
  • Restriction Digest/Heat Kill
  • PCR
  • Overnight Cultures and Glycerol Stocks
  • Plasmid Extraction Mini Prep
  • Plasmid Extraction Midi Prep
  • Ligation
  • Gel Extraction
  • Gel Electrophoresis
  • Ethanol Precipitate
  • Lab Prep/Recipes

    • Transforming pCB302-gfp-MBD into E. coli

    Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP

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