Difference between revisions of "Team:Georgia State/Protocols"
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<img src="https://static.igem.org/mediawiki/2019/thumb/d/d9/T--Georgia_State--pcb302_work_flow_into_Agro.png/503px-T--Georgia_State--pcb302_work_flow_into_Agro.png"></img> | <img src="https://static.igem.org/mediawiki/2019/thumb/d/d9/T--Georgia_State--pcb302_work_flow_into_Agro.png/503px-T--Georgia_State--pcb302_work_flow_into_Agro.png"></img> | ||
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<p>Codon Optimized RFP<p> | <p>Codon Optimized RFP<p> | ||
<p>Dino GFP<p> | <p>Dino GFP<p> | ||
<p>Dino RFP<p> | <p>Dino RFP<p> | ||
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Revision as of 01:34, 21 October 2019
put in the flow charts and create links to pdfs of our protocols
Transformation Protocols
Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
Symbiodinium microadriaticum Electroporation Transformations
Transforming A. tumefaciens
Transforming E.coli
ASP-8A Media
F2 Media
L1 Media
Restriction Digest/Heat Kill
PCR
Overnight Cultures and Glycerol Stocks
Plasmid Extraction Mini Prep
Plasmid Extraction Midi Prep
Ligation
Gel Extraction
Gel Electrophoresis
Ethanol Precipitate
Lab Prep/Recipes
Culturing Medias
Other Techniques
Transforming pCB302-gfp-MBD into E. coli
Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens
Codon Optimized RFP
Dino GFP
Dino RFP