Difference between revisions of "Team:Georgia State/Protocols"

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<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li>   
 
<li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li>   
 +
  
 
<h4>Culturing Medias</h4>
 
<h4>Culturing Medias</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li>  
  
<p>Restriction Digest/Heat Kill<p>
+
<h4>Other Techniques</h4>
<p>PCR<p>
+
<li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li>
<p>Overnight Cultures and Glycerol Stocks<p>
+
<li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li>
<p>Plasmid Extraction Mini Prep<p>
+
<li><a href="https://static.igem.org/mediawiki/2019/f/f0/T--Georgia_State--ovgs.pdf">Overnight Cultures and Glycerol Stocks</a></li>
<p>Plasmid Extraction Midi Prep<p>
+
<li><a href="https://static.igem.org/mediawiki/2019/8/8a/T--Georgia_State--miniprep.pdf">Plasmid Extraction Mini Prep</a></li>
<p>Ligation<p>  
+
<li><a href="https://static.igem.org/mediawiki/2019/8/82/T--Georgia_State--midiprep.pdf">Plasmid Extraction Midi Prep</a></li>
<p>Gel Extraction<p>  
+
<li><a href="https://static.igem.org/mediawiki/2019/b/bf/T--Georgia_State--ligation.pdf">Ligation</a></li>  
<p>Gel Electrophoresis<p>
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<li><a href="https://static.igem.org/mediawiki/2019/2/22/T--Georgia_State--gel_extraction.pdf">Gel Extraction</a></li>  
<p>Ethanol Precipitate<p>  
+
<li><a href="https://static.igem.org/mediawiki/2019/f/f3/T--Georgia_State--gel_electrophoresis.pdf">Gel Electrophoresis</a></li>
<p>Lab Prep/Recipes<p>
+
<li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li>
 +
<li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li>
  
<p>Transforming pCB302-gfp-MBD into Escherichia coli<p>
 
 
<img src="https://static.igem.org/mediawiki/2019/thumb/4/4d/T--Georgia_State--pcb302_work_flow_into_E._coli.png/510px-T--Georgia_State--pcb302_work_flow_into_E._coli.png"></img>
 
<img src="https://static.igem.org/mediawiki/2019/thumb/4/4d/T--Georgia_State--pcb302_work_flow_into_E._coli.png/510px-T--Georgia_State--pcb302_work_flow_into_E._coli.png"></img>
  

Revision as of 01:31, 21 October 2019

GSU iGEM

put in the flow charts and create links to pdfs of our protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli
  • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media
  • Other Techniques

  • Restriction Digest/Heat Kill
  • PCR
  • Overnight Cultures and Glycerol Stocks
  • Plasmid Extraction Mini Prep
  • Plasmid Extraction Midi Prep
  • Ligation
  • Gel Extraction
  • Gel Electrophoresis
  • Ethanol Precipitate
  • Lab Prep/Recipes
  • Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP