Difference between revisions of "Team:Mingdao/Basic Part"

Line 111: Line 111:
 
</p>
 
</p>
 
<h1 id="goal-c-btn">Biological function</h1>
 
<h1 id="goal-c-btn">Biological function</h1>
−
<p>Biological function</p>
 
 
<h3>Benzene oxidation</h3>
 
<h3>Benzene oxidation</h3>
 
<p>Phenol is a product from benzene oxidation which is catalyzed by CYP2E1. </p>
 
<p>Phenol is a product from benzene oxidation which is catalyzed by CYP2E1. </p>
Line 118: Line 117:
 
<p>We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then,  DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3.
 
<p>We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then,  DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3.
 
</p>
 
</p>
 +
<center><p>Construct of PliaI-RBS-CYP2E1-Tr/pSB1C3</p></center>
 +
<center><img src="https://static.igem.org/mediawiki/2019/e/e2/T--Mingdao--image15.png" alt="" style="max-width:800px;"></center>
  
  

Revision as of 11:56, 20 October 2019

HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Notebook
Contribution
Results
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM ⇗
Responsive image

Best Basic Part

CYP2E1 efficiency measure

Emerson reagent

Protocol

Reference

Best Basic parts

Part name:CYP2E1, rabbit cytochrome P450 2E1 ( optimized for Bacillus preferred codon)

Part cat. no.:BBa_K2932000

Biological function

Benzene oxidation

Phenol is a product from benzene oxidation which is catalyzed by CYP2E1.

Gene cloning and protein expression

We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then, DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3.

Construct of PliaI-RBS-CYP2E1-Tr/pSB1C3