Difference between revisions of "Team:Georgia State/Notebook"

     <td id="date" style="color:#02757d"><a href="061019.pdf"><span class="tool" data-tip="Extracted the plasmid, PCB302-gfp-MBD, off papers A & B then transformed it using heat shock into E.Coli. Click to see more.">10</span></td>

     <td id="date" style="color:#02757d "><a href="061119.pdf"><span class="tool" data-tip="Redid the transformations because the transformants overgrew in the kanamycin plates and performed overnight cultures of the PCB302 in E.Coli. Click to see more.">11</span></td>

     <td id="date"style="color:#02757d "><a href="061219.pdf"><span class="tool" data-tip="Performed minipreps on transformations made on 6/10/2019, replated transformations with parts from the registry, redid transformations for PCB302 with one shot and DH5a homemade E. Coli competent cells with kanamycin plates made on 6/11/2019, ran PCR on J23102 promoter sequence and K592009 coding sequence, and created overnight cultures with 3 J23102 promoter ampicillin plates and 1 K592009 coding sequence plate. Click to see more.">12</span></td>

     <td id="date"style="color:#02757d "><a href="061319.pdf"><span class="tool" data-tip="Ran gel electrophoresis on J23102 and K592009, performed a miniprep on the overnight cultures of the transformation samples made on 6/12/2019, performed overnight cultures on the transformations done on 6/12/2019, and performed an analytical restriction digests on blue chromoprotein (K592009) and RFP promotor (J23102), and ran a gel on J23102 and K592009. Click to see more.">13</span></td>

     <td id="date" style="color:#02757d"><a href="061419.pdf"><span class="tool" data-tip="Performed a mini prep on PCB302 plasmid in E. Coli from papers A & B and performed gel extraction on blue chromoprotein and RFP promotor from the analytical digest that was performed on 6/13/2019. Click to see more.">14</span></td>

     <td id="date" style="color:#02757d"><a href="061719.pdf"><span class="tool" data-tip="Simulated an artificial ligation of blue chromoprotein part into J23102 promoter and pcba1020-r0040 minus RFP on snap gene, ligated BP part with J23102 from the gel extraction, transformed the BP-promoter construct into DH5a chemically competent cells, transformed PCB302 into One Shot Top 10 chemically competent E. Coli cells from papers A&B, and performed restriction digest on PCB302 from previous mini-prepped samples on 6/14/2019 in E. Coli from papers A&B. Click to see more.">17</span></td>

     <td id="date" style="color:#02757d"><a href="061819.pdf"><span class="tool" data-tip="Ran a gel on the restriction digest for the PCB302 samples A &B from 6/17/2019 to verify if we have the correct parts, performed colony PCR on ligations from previous day, made overnight cultures on the ligations as well as the PCB302 plasmid in E. Coli from papers A & B, and created primers for PCB302 partial sequence on snapgene. Click to see more.">18</span></td>

     <td id="date" style="color:#02757d"><a href="061919.pdf"><span class="tool" data-tip="Ran a gel for colony PCR on BCP K592009 and J23102 promoter ligation and redid overnight cultures on PCB302 in E. Coli from papers A &B and on the BCP K592009 and J23102 promoter ligation. Click to see more">19</span></td>

     <td id="date" style="color:#02757d"><a href="062019.pdf"><span class="tool" data-tip="Performed minipreps on the overnights from the previous day, made glycerol stocks for the overnight cultures from the previous day, and prepared the ASP-8A media. Click to see more.">20</span></td>

     <td id="date" style="color:#02757d"><a href="062119.pdf"><span class="tool" data-tip="Prepared the ASP-8A media, performed restriction digest on the mini prepped ligation samples from 6/20/2019, performed PCR on PCB302 in E. Coli from papers A&B, and ran a gel for the restriction digest on the ligation. Click to see more.">21</span></td>

     <td id="date" style="color:#02757d"><a href="062419.pdf"><span class="tool" data-tip="Made PII Trace Metal mix, performed gel electrophoresis on PCR samples of PCB302 in E. Coli from papers A & B minipreps done on 6/20/2019, and fed the O. Marina. Click to see more.">24</span></td>

     <td id="date" style="color:#02757d"><a href="062519.pdf"><span class="tool" data-tip="Redid gel electrophoresis on PCR products of K592009 & J23102 ligations and the PCB302 in E. Coli from papers A & B, redid PCR on K592009 & J23102 ligations, performed restriction digest on PCB302 from mini preps in E. Coli from papers A & B from colony on 6/20/2019, made ASP-8A medium, did transformations on K592009 & J23102 ligation mix from 6/17/2019, and fed O. Marina. Click to see more.">25</span></td>

     <td id="date" style="color:#02757d"><a href="062619.pdf"><span class="tool" data-tip="Ran a gel on restriction digest of PCB302 from the miniprep colony 7 in E. Coli from papers A & B from 6/20/2019 and on the PCR of ligations of K592009 and J23102 from 6/25/2019, checked on the algae, filtered some seawater, checked the ligation transformations from 6/25/2019 for growth, and fed the O. Marina. Click to see more.">26</span></td>

     <td id="date" style="color:#02757d"><a href="062719.pdf"><span class="tool" data-tip="Transferred O. Marina and D. Tertiolecta into filtered, autoclaved seawater, filtered and autoclaved more seawater, verified algal life, and fed the O. Marina. Click to see more.">27</span></td>

     <td id="date" style="color:#02757d"><a href="062819.pdf"><span class="tool" data-tip="Ran a PCR on the PCB302 plasmid straight from paper solution A & B and transferred O. Marina, S. Microadiatic, D. Tertiolecta into other cultures and observed the algae. Click to see more.">28</span></td>