Difference between revisions of "Team:Georgia State/Protocols"

Revision as of 19:37, 19 October 2019

GSU iGEM

put in the flow charts and create links to pdfs of our protocols

Link pdfs for all below, listed in order on wiki uploads sheet

Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum

4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum

Symbiodinium microadriaticum Electroporation Transformations

ASP-8A Media

F2 Media

L1 Media

Transforming E.coli

Transforming A. tumefaciens

Restriction Digest/Heat Kill

PCR

Overnight Cultures and Glycerol Stocks

Plasmid Extraction Mini Prep

Plasmid Extraction Midi Prep

Ligation

Gel Extraction

Gel Electrophoresis

Ethanol Precipitate

Lab Prep/Recipes

Transforming pCB302-gfp-MBD into Escherichia coli

Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

Codon Optimized RFP

Dino GFP

Dino RFP

@@ Line 114: / Line 114: @@
 <p>put in the flow charts and create links to pdfs of our protocols<p>
+<p>Link pdfs for all below, listed in order on wiki uploads sheet<p>
+<p>Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum<p>
+<p>4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum<p>
+<p>Symbiodinium microadriaticum Electroporation Transformations<p>
+<p>ASP-8A Media<p>
+<p>F2 Media<p>
+<p>L1 Media<p>
+<p>Transforming E.coli<p>
+<p>Transforming A. tumefaciens<p>
+<p>Restriction Digest/Heat Kill<p>
+<p>PCR<p>
+<p>Overnight Cultures and Glycerol Stocks<p>
+<p>Plasmid Extraction Mini Prep<p>
+<p>Plasmid Extraction Midi Prep<p>
+<p>Ligation<p>
+<p>Gel Extraction<p>
+<p>Gel Electrophoresis<p>
+<p>Ethanol Precipitate<p>
+<p>Lab Prep/Recipes<p>
 <p>Transforming pCB302-gfp-MBD into Escherichia coli<p>
 <img src="https://static.igem.org/mediawiki/2019/thumb/4/4d/T--Georgia_State--pcb302_work_flow_into_E._coli.png/510px-T--Georgia_State--pcb302_work_flow_into_E._coli.png"></img>