Difference between revisions of "Team:Georgia State/Protocols"
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<p>put in the flow charts and create links to pdfs of our protocols<p> | <p>put in the flow charts and create links to pdfs of our protocols<p> | ||
<a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a> | <a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a> | ||
| − | < | + | <a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a> |
<p>Symbiodinium microadriaticum Electroporation Transformations<p> | <p>Symbiodinium microadriaticum Electroporation Transformations<p> | ||
<p>ASP-8A Media<p> | <p>ASP-8A Media<p> | ||
Revision as of 01:04, 21 October 2019
put in the flow charts and create links to pdfs of our protocols
Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
Symbiodinium microadriaticum Electroporation Transformations
ASP-8A Media
F2 Media
L1 Media
Transforming E.coli
Transforming A. tumefaciens
Restriction Digest/Heat Kill
PCR
Overnight Cultures and Glycerol Stocks
Plasmid Extraction Mini Prep
Plasmid Extraction Midi Prep
Ligation
Gel Extraction
Gel Electrophoresis
Ethanol Precipitate
Lab Prep/Recipes
Transforming pCB302-gfp-MBD into Escherichia coli
Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens
Codon Optimized RFP
Dino GFP
Dino RFP