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<p>We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then, DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3. | <p>We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then, DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3. | ||
</p> | </p> | ||
− | < | + | <p><center>Construct of PliaI-RBS-CYP2E1-Tr/pSB1C3</center></p> |
<center><img src="https://static.igem.org/mediawiki/2019/e/e2/T--Mingdao--image15.png" alt="" style="max-width:800px;"></center> | <center><img src="https://static.igem.org/mediawiki/2019/e/e2/T--Mingdao--image15.png" alt="" style="max-width:800px;"></center> | ||
Revision as of 11:59, 20 October 2019
![Responsive image](https://static.igem.org/mediawiki/2019/8/8c/T--Mingdao--BP.png)
Best Basic Part
CYP2E1 efficiency measure
Emerson reagent
Protocol
Reference
Best Basic parts
Part name:CYP2E1, rabbit cytochrome P450 2E1 ( optimized for Bacillus preferred codon)
Part cat. no.:BBa_K2932000
![](https://static.igem.org/mediawiki/2019/4/4b/T--Mingdao--image21.png)
Biological function
Benzene oxidation
Phenol is a product from benzene oxidation which is catalyzed by CYP2E1.
![](https://static.igem.org/mediawiki/2019/6/6e/T--Mingdao--image29.png)
Gene cloning and protein expression
We optimized the gene codon of rabbit cytochrome P450 2E1 (CYP2E1) based on Bacillus subtilis codon usage preference and synthesized the DNA fragment by Twist Bioscience. Then, DNA fragments were amplified by PCR and assembled with a terminator, followed by inserting to PliaI-RBS/pSB1C3.
![](https://static.igem.org/mediawiki/2019/e/e2/T--Mingdao--image15.png)