Difference between revisions of "Team:Georgia State/Notebook"

   <td id="date" style="color:#02757d"><a href="070119.pdf"><span class="tool" data-tip="Transformed new samples of PCB302 (papers 1 & 2) into E. Coli, transformed blue chromoprotein composite, made YM media plate with kanamycin and YM broth, verified algal life, ran gel on PCR of PCB302 in E. Coli from papers A & B from 6/28/2019, and checked concentrations on PCB302 from new samples. Click to see more.">1</span></td>

   <td id="date" style="color:#02757d"><a href="070219.pdf"><span class="tool" data-tip="Made YM media plates with kanamycin, transformed PCB302 plasmid into agrobacterium tumefaciens, made overnight cultures from PCB302 in E. Coli transformations, verified algal life, transformed codon optimized RFP in E. Coli, and performed colony PCR on PCB302 in E. Coli. Click to see more.">2</span></td>

   <td id="date" style="color:#02757d"><a href="070319.pdf"><span class="tool" data-tip="Created new media for the algae, made glycerol stocks, ran a PCR analysis of PCB302 in E. Coli from papers 1 & 2, transformed blue chromoprotein into E. Coli, performed a miniprep on the PCB302 in E. Coli from papers 1 & 2, and fed O. Marina. Click to see more.">3</span></td>

   <td id="date" style="color:#02757d"><a href="070419.pdf"><span class="tool" data-tip="Ran a restriction digest of PCB302 in E. Coli from papers 1 & 2, performed gel electrophoresis analysis of PCB302 in E. Coli papers 1 & 2 digest, made cultures for the S. microadriaticum in L1 and L1+F2, and prepared ASP-8A media with filtered seawater. Click to see more.">4</span></td>

   <td id="date" style="color:#02757d"><a href="070519.pdf"><span class="tool" data-tip="Ran a PCR on PCB302 in E. Coli straight from papers 1 & 2 and made overnight cultures of PCB302 in Agrobacterium Tumefaciens from papers 1 & 2. Click to see more.">5</span></td>

   <td id="date" style="color:#02757d"><a href="070819.pdf"><span class="tool" data-tip="Performed minipreps on the PCB302 in Agrobacterium tumefaciens from papers 1 & 2, made glycerol stocks for overnight PCB302 Agrobacterium cultures papers 1 & 2, and determined algae concentration. Click to see more.">8</span></td>

   <td id="date" style="color:#02757d"><a href="070919.pdf"><span class="tool" data-tip="Ran gel on PCR products of PCB302-gfp-MBD in E. Coli from papers 1 & 2, transformed codon optimized RFP into E. Coli, transformed blue chromoprotein into E. Coli, performed PCR on PCB302 in A. Tumefaciens from miniprep on 7/8/2019, ran gel on PCR of PCB302 in A. Tumefaciens from miniprep, transformed PCB302 into A. Tumefaciens from papers 1 & 2, and made overnight cultures of PCB302 in A. Tumefaciens from glycerol stocks of minipreps on 7/8/2019. Click to see more.">9</span></td>

   <td id="date" style="color:#02757d"><a href="071019.pdf"><span class="tool" data-tip="Performed PCR on PCB302 from papers 1 & 2, made overnight cultures of codon-optimized-RFP from transformations on 7/9/2019, performed transformations on new blue chromoprotein, and performed gel electrophoresis on the PCB302 PCR from today. Click to see more.">10</span></td>

   <td id="date" style="color:#02757d"><a href="071119.pdf"><span class="tool" data-tip="Performed miniprep on overnight cultures of codon optimized RFP from 7/10/2019, made glycerol stocks on overnight cultures of codon optimized RFP, ran restriction digest on codon optimized RFP mini preps, ran a gel on digested codon optimized RFP, cultured algae in ASP-8A with seawater, and made plates for Symbiodinium culture. Click to see more.">11</span></td>

   <td id="date" style="color:#02757d"><a href="071219.pdf"><span class="tool" data-tip="Picked colonies from PCB302 Agrobacterium transformations from papers 1 & 2, made overnight cultures on PCB302 Agrobacterium transformations, performed minipreps on PCB302 in A. Tumefaciens’ glycerol stocks made on 7/8/2019, ran restriction digest on codon-optimized-RFP, performed colony PCR on blue chromoprotein, and made overnight cultures on PCB302 in A. Tumefaciens transformations from 7/9/2019. Click to see more.">12</span></td>

   <td id="date" style="color:#02757d"><a href="071519.pdf"><span class="tool" data-tip="Made glycerol stock for overnight cultures of transformations done on 7/9/2019, performed miniprep on PCB302 in A. Tumefaciens’ overnight cultures from 7/9/2019, did transformations on blue chromoprotein and DinoIII part 2, ran gel on blue chromoprotein colony PCR, and made overnight cultures on pGEX-HCG from glycerol stocks. Click to see more.">15</span></td>

   <td id="date" style="color:#02757d"><a href="071619.pdf"><span class="tool" data-tip="Ran PCR on PCB302 from papers 1 & 2 and on PCB302 in A. tumefaciens from miniprep done on 7/15/2019 with new primers, made overnight cultures on PCB302 in A. Tumefaciens’ glycerol stocks made on 7/15/2019, the pGEX-HCG glycerol stocks, and DinoIIIP2, and ran a gel on the miniprep of PCB302 in A. Tumefaciens from 7/15/2019. Click to see more.">16</span></td>

   <td id="date" style="color:#02757d"><a href="071719.pdf"><span class="tool" data-tip="Did minipreps on DinoIII-GFP part 2 and Pgex-HCG, made glycerol stocks for DinoIII-GFP Part 2, did transformations on PCB302 in A. Tumefaciens from papers 1 &2, ran restriction digest on codon-optimized-RFP, and made overnight cultures on blue chromoprotein. Click to see more.">17</span></td>

   <td id="date" style="color:#02757d"><a href="071819.pdf"><span class="tool" data-tip="Did a miniprep on blue chromoprotein overnight cultures and PCB302 in A. Tumefaciens glycerol stocks that were made on 7/15/2019, and did gel electrophoresis on DinoIIIP2 and pGEX-HCG. Click to see more.">18</span></td>

   <td id="date" style="color:#02757d"><a href="071919.pdf"><span class="tool" data-tip="Made overnight cultures of PCB302 in A. Tumefaciens transformations from 7/17/2019, ran restriction digest on DinoIII-P2 with BgIII and XbaI, and did gel electrophoresis on DinoIII-P2 and the codon optimized RFP digested with BgIII and XbaI. Click to see more.">19</span></td>

   <td id="date" style="color:#02757d"><a href="072219.pdf"><span class="tool" data-tip="Performed miniprep on PCB302 in A. Tumefaciens’ transformations on 7/17/2019, made glycerol stocks of the overnights of PCB302 in A. Tumefaciens from 7/19/2019, ran a gel with BgIII and XbaI on DinoIII P2 and on codon optimized RFP, made overnights of PCB302 in A. Tumefaciens’ glycerol stocks from an unknown date, glycerol stocks of blue chromoprotein from an unknown date, and glycerol stocks of codon-optimized-RFP from an unknown date, and ran restriction digest with new XbaI and BgIII on DinoIII P2 and codon-optimized-RFP. Click to see more.">22</span></td>

   <td id="date" style="color:#02757d"><a href="072319.pdf"><span class="tool" data-tip="Performed miniprep on glycerol stocks of blue chromoprotein from an unknown date and glycerol stocks of codon-optimized-RFP from an unknown date, ran restriction digest on DinoIII P2 and codon-optimized-RFP, performed gel extraction to remove GFP from DinoIII P2 and isolate RFP from codon-optimized-RFP, and made overnights in 100 mL of glycerol stocks of blue chromoprotein from an unknown date, glycerol stocks of codon-optimized-RFP from an unknown date, and glycerol stocks of PCB302 in E. Coli made on 7/3/2019. Click to see more.">23</span></td>

   <td id="date" style="color:#02757d"><a href="072419.pdf"><span class="tool" data-tip="Performed MiDi prep on bluechromoprotein and codon-optimized-RFP, and made YM media. Click to see more.">24</span></td>

   <td id="date" style="color:#02757d"><a href="072519.pdf"><span class="tool" data-tip="Made overnight cultures of glycerol stocks of DinoIII-GFP P2, performed MiDi prep on glycerol stocks of PCB302 in E. Coli made on 7/3/2019, transformed improved mCherry into E. Coli, and sent K1357009 and J23102 for sequencing. Click to see more.">25</span></td>

   <td id="date" style="color:#02757d"><a href="072619.pdf"><span class="tool" data-tip="Performed MiDi prep on DinoIII-GFP P2 and finished the MiDi prep on the glycerol stocks of PCB302 in E. Coli from papers 1 & 2 made on 7/3/2019. Click to see more.">26</td>

   <td id="date" style="color:#02757d"><a href="072919.pdf"><span class="tool" data-tip="Ran a restriction digest on DinoIII-GFP P2 and made overnight cultures for PCB302 in E. Coli glycerol stocks that were made on 7/3/2019. Click to see more.">29</td>

   <td id="date" style="color:#02757d"><a href="072919.pdf"><span class="tool" data-tip="Performed mini prep on PCB302 in E. Coli overnight cultures made from 7/3/2019, ran restriction digest on the PCB302 miniprep samples from today, on the DinoIII plasmid, and on the codon-optimized-RFP. Click to see more.">31</td>

<td id="date"><a href="080119"><span class="tool" data-tip="Performed gel extraction of DinoIII, did transformations on mCherry, did colony PCR on PCB302 using gfp primers, primers 3 and 4, did PCR on the source of DNA for the PCB302 using gfp primers, primers 3 and 4, ran an E-gel on the gel extraction product, and ran a restriction digest on RFP for ligation. Click to see more.">1</td>

<td id="date"><a href="080219"><span class="tool" data-tip="Ran a gel on the PCR products from yesterday, ran a gel and performed gel extraction on the digested codon optimized RFP, did ligation RFP and DinoIII plasmid, and ligation for the composite part. Click to see more.">2</td>

<td id="date"><a href="080519"><span class="tool" data-html="true" data-tip="Linearize DinoIII with GFP miniprep, cultured a larger volume of Symbiodinium and Oxyrrhis Marina, and filtered and autoclaved seawater. Click to see more.">5</td>

<td id="date"><a href="080619"><span class="tool" data-tip="Did heat kill on the restriction digested Dino III mini prep samples #1 and #10 from 7/17/2019, performed gel extraction on linearized Dino III with GFP, transformed DinoIII with the codon optimized RFP into E. Coli using OneShot chemically competent cells. Click to see more.">6</td>

<td id="date"><a href="080719"><span class="tool" data-tip="Transformed mCherry into BL21 and NEBa cells, did PCR on the DinoIII-rfp colonies from the transformations that were done on 8/6/2019, ran a restriction digest on codon optimized RFP in a 200 µL reaction, and made overnight cultures on the ligation of DinoIII and RFP. Click to see more.">7</td>

<td id="date"><a href="080819"><span class="tool" data-tip="Mini prepped DinoIII with RFP from 8/6/2019 transformations, ran a gel on PCR of DinoIII with RFP transformation, performed ethanol precipitation on codon optimized RFP digest from 8/7/2019, made O. Marina cultures, made glycerol stocks of DinoIII-RFP from 8/6/2019 transformations, and sent DinoIII-GFP, Dino-RFP, and codon optimized RFP for sequencing. Click to see more.">8</td>

<td id="date-pdf">10</td>