Difference between revisions of "Team:Georgia State/Composite Part"

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<html>
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<html lang="en">
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<link rel="stylesheet" type="text/css" href="https://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700|Playfair+Display:400,700&action=raw&ctype=text/css">
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    <title>Composite Part</title>
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  <style>
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  .col-12{
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    word-break: break-word;
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  }
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  </style>
  
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</head>
  
  
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<body>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                                    <li class="active"><a href="https://2019.igem.org/wiki/index.php?title=Team:Georgia_State">Home</a></li>
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                                    <li><a href="../Team:Georgia_State/Team">Team</a>
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<ul class="dropdown">
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    <li><a href="../Team:Georgia_State/Team">- Team Members</a></li>
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    <li><a href="../Team:Georgia_State/Collaborations">- Collaborations</a></li>
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</ul>
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                                            <li><a href="../Team:Georgia_State/Description">- Description</a></li>
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                                            <li><a href="../Team:Georgia_State/Design">- Design</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Culturing">- Culturing</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Notebook">- Notebook</a></li>
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                                            <li><a href="../Team:Georgia_State/Protocols">- Protocols</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Results">- Results</a></li>
 +
                                            <li><a href="../Team:Georgia_State/AlgaeObservations">- Algae Observations</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Hardware">- Hardware</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Model">- Model</a></li>
 +
                                        </ul>
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                                    </li>
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<ul class="dropdown">
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                                            <li><a href="../Team:Georgia_State/Contribution">- Contribution</a></li>
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                                            <li><a href="../Team:Georgia_State/Basic_Part">- Basic Parts</a></li>
 +
                                            <li><a href="../Team:Georgia_State/Composite_Part">- Composite Parts</a></li>
 +
                                    </ul>
 +
</li>
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                                    <li><a href="../Team:Georgia_State/Human_Practices">Human Practices</a>
 +
<ul class="dropdown">
 +
    <li><a href="../Team:Georgia_State/Human_Practices">- Human Practices</a></li>
 +
    <li><a href="../Team:Georgia_State/Public_Engagement">- Public Engagement</a></li>
 +
</ul></li>
 +
                                    <li><a href="../Team:Georgia_State/Safety">Safety</a></li>
 +
                                    <li><a href="https://igem.org/2019_Judging_Form?team=Georgia_State">Judging Form</a></li>
 +
                               
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                <div class="col-12">
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                    <div class="breadcrumb-content">
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                        <h2>Composite Parts</h2>
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                                <li class="breadcrumb-item"><a href="../Team:Georgia_State"><i class="icon_house_alt"></i> Home</a></li>
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<li class="breadcrumb-item"><a href="../Team:Georgia_State/Parts">Parts</a></li>
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<li class="breadcrumb-item"><a href="../Team:Georgia_State/Contribution">Contribution</a></li>
 +
<li class="breadcrumb-item"><a href="../Team:Georgia_State/Basic_Part">Basic</a></li>
 +
                                <li class="breadcrumb-item active" aria-current="page">Composite Parts</li>
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<div class="table responsive">
 +
<table class="table-striped table-bordered w-auto overflow-auto" height="500" width="500">
 +
<tr align="align">
 +
<th colspan="2"><h4>Composite Parts</h4></th>
 +
</tr>
 +
<td><h6>Dino III GFP Plasmid</h6></td>
 +
<td><li><a href="http://parts.igem.org/Part:BBa_K3042005">Part:BBa_K3042005</a></li></td>
 +
</tr>
 +
<tr><td><h6>Dino III RFP Plasmid</h6></td>
 +
<td><li><a href="http://parts.igem.org/Part:BBa_K3042006">Part:BBa_K3042006</a></li></td>
 +
</tr>
 +
<tr><td><h6>pCB302-gfp-MBD plasmid</h6></td>
 +
<td><h6>*This part is not in the registry*</h6></td>
 +
</table>
 +
</div>
 
</div>
 
</div>
  
 +
</div>
 +
<h1>pCB302-gfp-MBD Plasmid</h1>
 +
<div class="row">
 +
<div class="col-6">
 +
<p>Our predicted sequence created in SnapGene<p>
 +
<img src="https://static.igem.org/mediawiki/2019/6/6b/T--Georgia_State--pCB302gfpMBD.png"></img>
 +
</div>
 +
<div class="col-6">
 +
<p>The actual plasmid map<p>
 +
<img src="https://static.igem.org/mediawiki/2019/a/ab/T--Georgia_State--pcbplasmid.png"></img>
 +
</div>
 +
</div>
  
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<div class="clear"></div>
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<p><b><u>pCB302-GFP-MBD Binary Vector</b></u></p>
  
 +
<p>pCB302 is a plant targeted plasmid containing gfp encoding fusions with a microtubule-binding domain (MBD) . pCB302-GFP-MBD Binary Vector is harbored in Agrobacterium tumefaciens to evaluate the heterologous expression of the photosynthetic dinoflagellate, Symbiodinium mircoadriaticum. The plasmid was derived from Dr. Boris Voigt from The Institute for Cellular and Molecular Botany, University of Bonn, Germany who donated it to the Instituto de Biotecnología, UNAM (Cuernavaca, Morelos, México) who then donated it to iGEM GSU.
 +
After pCB302-GFP-MBD in Agrobacterium tumefaciens was introduced into the Symbiodinium mircoadriaticum, the cells lost their photosynthetic pigments and were left unable to reproduce. A successful transformation of the Symbiodinium mircoadriaticum, done by UNAM,  was measured by GFP expression frequency measured as green fluorescence emission yielding 640 per every 1,000,000 cells. Despite losing photosynthetic ability, pCB302-GFP-MBD Binary Vector is the first step to a stable Symbiodinium mircoadriaticum transformation (Ortiz-Matamoros, 2015).</p>
  
 +
<dl>
 +
<dt>LB- LB T-DNA repeat</dt>
 +
<dd>Left border repeat from nopaline C58 T-DNA</dd>
  
 +
</dl>
  
 +
<dl>
 +
<dt>OriV- incP</dt>
 +
<dd>Origin of replication</dd>
  
−
<div class="column full_size">
+
</dl>
−
<h1>Composite Parts</h1>
+
  
 +
<dl>
 +
<dt>nptIII-</dt>
 +
<dd>Kanamycin resistance gene</dd>
  
−
<p>
 
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
−
</p>
 
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
<dl>
−
</div>
+
<dt>TrfA-</dt>
 +
<dd>Gene for trans-acting replication protein that binds to and activates OriV</dd>
 +
 
 +
<dl>
 +
<dt>MBD- Microtubule binding domain</dt>
 +
<dd>of the mammalian microtubule-associated protein 4 (MAP4) gene</dd>
 +
</dl>
 +
 
 +
<dl>
 +
<dt>GFP- Green fluorescent protein;Plant optimized</dt>
 +
<dd>Reporter gene</dd>
 +
<dd>Binds to microtubules in vitro when fused to MBD</dd>
 +
</dl>
 +
 
 +
<dl>
 +
<dt>Primers for PCR</dt>
 +
<dd>5’-GATGAGTAAAGGAGAAGAAC-3’</dd>
 +
<dd>5’-TAT TTGTATAGTTCATCCATGCC-3’</dd>
 +
</dl>
 +
 
 +
<dl>
 +
<dt>Tnos-</dt>
 +
<dd>Nopaline Synthase Terminator and poly(A) signal</dd>
 +
 
 +
<dl>
 +
<dt>Bar-</dt>
 +
<dd>Phosphinothricin acetyl transferase</dd>
 +
<dd>Confers resistance to bialophos or phosphinothricin </dd>
 +
 
 +
<dl>
 +
<dt>Pnos-</dt>
 +
<dd>Nopaline Synthase Promoter</dd>
 +
<dl>
 +
<dt>RB- RB T-DNA repeat</dt>
 +
<dd>Right border repeat from nopaline C58 T-DNA</dd>
 +
 
 +
 
 +
<dl>
 +
<dt>Forward Primer:</dt>
 +
 
 +
 
 +
<dl>
 +
<dt>Reverse primers:</dt>
 +
<dd>5’-GCACTAGTGTAACGACCAATACCCCA-3’</dd>
 +
</dl>
 +
 
  
−
<div class="column full_size">
+
<p>Ortiz-Matamoros, M. F., Islas-Flores, T., Voigt, B., Menzel, D., Baluška, F., & Villanueva, M. A. (2015). Heterologous DNA Uptake in Cultured Symbiodinium spp. Aided by Agrobacterium tumefaciens. PloS one, 10(7), e0132693. doi:10.1371/journal.pone.0132693<p>
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<div class="highlight decoration_background">
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−
<h3>Note</h3>
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−
<p>This page should list all the composite parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
+
 
</div>
 
</div>
 
</div>
 
</div>
 +
</div>
 +
</section>
 +
</div>
 +
</div>
 +
<!-- Goodbye Area Start -->
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    <section class="akame-service-area">
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 +
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−
<div class="column full_size">
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                        <!-- Copywrite Text -->
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<h3>Best Composite Part Special Prize</h3>
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                            <p> <!-- Link back to Colorlib can't be removed. Template is licensed under CC BY 3.0. -->
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Copyright &copy;<script>document.write(new Date().getFullYear());</script> All rights reserved | This template is made with <i class="fa fa-heart-o" aria-hidden="true"></i> by <a href="https://colorlib.com" target="_blank">Colorlib</a> | Modified by GSU iGEM
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                    </div>
 +
                </div>
  
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<p>To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
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−
<br><br>
+
                                             
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
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 +
                </div>
  
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                            <p>Tel: (+1) 404 413 5344</p>
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Latest revision as of 03:39, 22 October 2019

Composite Part

Composite Parts

Composite Parts
Dino III GFP Plasmid
  • Part:BBa_K3042005
    		•
    Dino III RFP Plasmid
  • Part:BBa_K3042006
    		•
    pCB302-gfp-MBD plasmid
    *This part is not in the registry*

    pCB302-gfp-MBD Plasmid

    Our predicted sequence created in SnapGene

    The actual plasmid map

    pCB302-GFP-MBD Binary Vector

    pCB302 is a plant targeted plasmid containing gfp encoding fusions with a microtubule-binding domain (MBD) . pCB302-GFP-MBD Binary Vector is harbored in Agrobacterium tumefaciens to evaluate the heterologous expression of the photosynthetic dinoflagellate, Symbiodinium mircoadriaticum. The plasmid was derived from Dr. Boris Voigt from The Institute for Cellular and Molecular Botany, University of Bonn, Germany who donated it to the Instituto de Biotecnología, UNAM (Cuernavaca, Morelos, México) who then donated it to iGEM GSU. After pCB302-GFP-MBD in Agrobacterium tumefaciens was introduced into the Symbiodinium mircoadriaticum, the cells lost their photosynthetic pigments and were left unable to reproduce. A successful transformation of the Symbiodinium mircoadriaticum, done by UNAM, was measured by GFP expression frequency measured as green fluorescence emission yielding 640 per every 1,000,000 cells. Despite losing photosynthetic ability, pCB302-GFP-MBD Binary Vector is the first step to a stable Symbiodinium mircoadriaticum transformation (Ortiz-Matamoros, 2015).

    LB- LB T-DNA repeat
    Left border repeat from nopaline C58 T-DNA
    OriV- incP
    Origin of replication
    nptIII-
    Kanamycin resistance gene
    TrfA-
    Gene for trans-acting replication protein that binds to and activates OriV
    MBD- Microtubule binding domain
    of the mammalian microtubule-associated protein 4 (MAP4) gene
    GFP- Green fluorescent protein;Plant optimized
    Reporter gene
    Binds to microtubules in vitro when fused to MBD
    Primers for PCR
    5’-GATGAGTAAAGGAGAAGAAC-3’
    5’-TAT TTGTATAGTTCATCCATGCC-3’
    Tnos-
    Nopaline Synthase Terminator and poly(A) signal
    Bar-
    Phosphinothricin acetyl transferase
    Confers resistance to bialophos or phosphinothricin
    Pnos-
    Nopaline Synthase Promoter
    RB- RB T-DNA repeat
    Right border repeat from nopaline C58 T-DNA
    Forward Primer:
    Reverse primers:
    5’-GCACTAGTGTAACGACCAATACCCCA-3’

    Ortiz-Matamoros, M. F., Islas-Flores, T., Voigt, B., Menzel, D., Baluška, F., & Villanueva, M. A. (2015). Heterologous DNA Uptake in Cultured Symbiodinium spp. Aided by Agrobacterium tumefaciens. PloS one, 10(7), e0132693. doi:10.1371/journal.pone.0132693

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