Difference between revisions of "Team:SUIS Shanghai/Basic Part"
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| − | <h3>Parts Table</h3> | + | <h3>Basic Parts Table</h3> |
| + | <h4>This year our teams created five new basic parts our of our total collection of 21. We feel our best part is <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031015">BBa_K3031015</a> which is a variant of the luxI promoter regulatory sequence from the commonly used Quorum sensing bacterial system. We added a Ferric Uptake Regulator (FUR) sequence into this promoter meaning that when this is used as intended in the LuxR/LuxI system, the FUR sequence can inhibit the regulation of genes under the lux promoter in low iron concentrations. Please visit the part page for more details on its Biology and Usage as well as experiments used to characterize and validate this part.</h4> | ||
| + | <h4>We also created a new part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031018">BBa_K3031018</a>) which is a mutated sequence of a previously existing transcription factor part which is activated when in the presence of Reactive Oxygen Species (ROS). This part was also experimentally validated and characterized on its part page on the registry. The other basic parts are coding regions associated specifically with our project and have applications in the development of live bacterial vaccine delivery systems.</h4> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031015">BBa_K3031015</a></td> |
| − | <td> | + | <td>LuxI promoter with FUR box located in the -10 region</td> |
| − | <td> | + | <td>166</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031018">BBa_K3031018</a></td> |
| − | <td> | + | <td>OxyR mutated at residues 147 & 203</td> |
| + | <td>915</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031000">BBa_K3031000</a></td> |
| − | <td> | + | <td>ORF81 - Membrane envelpe protein of Cyprinid Herpesvirus 3</td> |
| + | <td>959</td> | ||
</tr> | </tr> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031001">BBa_K3031001</a></td> |
| − | <td> | + | <td>CwA - Cell wall anchoring whole protein from <i>Lactobacillus plantarum</i></td> |
| + | <td>2118</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3031002">BBa_K3031002</a></td> |
| − | <td> | + | <td>CwA (194aa) - Truncated cell wall anchoring protein from <i>Lactobacillus plantarum</i> truncated sequence</td> |
| − | + | <td>678</td> | |
| − | + | ||
| − | + | ||
| − | + | ||
</tr> | </tr> | ||
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</table> | </table> | ||
Latest revision as of 01:33, 22 October 2019
PARTS
HUMAN PRACTICES
SUIS_Shanghai 2019 Basic Parts
Basic Parts Table
This year our teams created five new basic parts our of our total collection of 21. We feel our best part is BBa_K3031015 which is a variant of the luxI promoter regulatory sequence from the commonly used Quorum sensing bacterial system. We added a Ferric Uptake Regulator (FUR) sequence into this promoter meaning that when this is used as intended in the LuxR/LuxI system, the FUR sequence can inhibit the regulation of genes under the lux promoter in low iron concentrations. Please visit the part page for more details on its Biology and Usage as well as experiments used to characterize and validate this part.
We also created a new part (BBa_K3031018) which is a mutated sequence of a previously existing transcription factor part which is activated when in the presence of Reactive Oxygen Species (ROS). This part was also experimentally validated and characterized on its part page on the registry. The other basic parts are coding regions associated specifically with our project and have applications in the development of live bacterial vaccine delivery systems.
| Part Name | Part Description | Length |
|---|---|---|
| BBa_K3031015 | LuxI promoter with FUR box located in the -10 region | 166 |
| BBa_K3031018 | OxyR mutated at residues 147 & 203 | 915 |
| BBa_K3031000 | ORF81 - Membrane envelpe protein of Cyprinid Herpesvirus 3 | 959 |
| BBa_K3031001 | CwA - Cell wall anchoring whole protein from Lactobacillus plantarum | 2118 |
| BBa_K3031002 | CwA (194aa) - Truncated cell wall anchoring protein from Lactobacillus plantarum truncated sequence | 678 |