Difference between revisions of "Team:Mingdao/Achievement"

 
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<h2>Competition Deliverables</h2>
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<li>All deliverables were met.</li>
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          <a href="https://2018.igem.org/Team:USTC">HOME</a>
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          <a href="#projectSubmenu" data-toggle="collapse" aria-expanded="false">PROJECT <i class="fa fa-caret-down"></i></a>
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<a href="https://2019.igem.org/Team:Mingdao/Attributions"><h2>Attributions</h2></a>
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<li>You can check out what our team did and how others helped our project.</li>
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              <a href="https://2018.igem.org/Team:USTC/Description">Description</a>
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              <a href="https://2018.igem.org/Team:USTC/Design">Design</a>
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<a href="https://2019.igem.org/Team:Mingdao/Description"><h2>Project Inspiration and Description</h2></a>
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<li>We introduce what inspires us and what we have done on our project. Check out on our Description Page</li>
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              <a href="https://2018.igem.org/Team:USTC/Results">Results</a>
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              <a href="https://2018.igem.org/Team:USTC/Hardware">Hardware</a>
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/d/de/T--Biomarvel--achi_silver.png"style="display:width=400px; margin:auto; margin-left:44%;" />
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              <a href="https://2018.igem.org/Team:USTC/Safety">Safety</a>
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<a href="https://2019.igem.org/Team:Mingdao/Notebook"><h2>Parts</h2></a>
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<li>We submitted quantitative experimental characterization data to an existing Part from the Registry of Standard Biological Parts .</li>
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              <a href="https://2018.igem.org/Team:USTC/Parts">Parts</a>
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<li>Validation can be found on our result page and registry.</li>
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<a href="https://2019.igem.org/Team:Mingdao/Human_Practices"><h2>Human Practices</h2></a>
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          <a href="#modelSubmenu" data-toggle="collapse" aria-expanded="false">MODEL <i class="fa fa-caret-down"></i></a>
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<li>We have investigated related issues and engaged with our relevant communities.</li>
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              <a href="https://2018.igem.org/Team:USTC/Model">Overview</a>
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<a href="https://2019.igem.org/Team:Mingdao/Collaborations"><h2>Collaboration</h2></a>
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<ul>
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              <a href="https://2018.igem.org/Team:USTC/Model/Single_Cell_Model">Single-cell Model</a>
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<li>We have significantly worked with currently registered 2019 iGEM teams in a meaningful way.</li>
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            </li>
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</ul>
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            <li>
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              <a href="https://2018.igem.org/Team:USTC/Model/System_Analysis">System Analysis</a>
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<section id="mSection">
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/4/4a/T--Biomarvel--achi_gold.png" style="display:block; margin:auto;" />
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              <a href="https://2018.igem.org/Team:USTC/Model/Performance_evaluation">Performance Evaluation</a>
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            </li>
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<a href="https://2019.igem.org/Team:Mingdao/Human_Practices"><h2>Integrated Human Practices</h2></a>
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    <li>
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<ul>
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              <a href="https://2018.igem.org/Team:USTC/Model/Parameters">Parameters</a>
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<li>We expanded on our silver medal activity by demonstrating how we have integrated the investigated issues into our project. Integrated human practices helped to improve our design and execution of the project.</li>
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<a href="https://2019.igem.org/Team:Mingdao/Model"><h2>Model Our Project</h2></a>
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          <a href="https://2018.igem.org/Team:USTC/InterLab">INTERLAB</a>
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<li> The model we developed was impactful on the design of our experiment and prototype. Our model demonstrated the effectiveness of the human Carbonic Anhydrase (CA) II enzyme in dissolving carbon dioxide in water and reducing the indoor carbon dioxide concentration, helping to prove the feasibility of our project design during initial testing prior to experiments. A mathematical correlation was found between the inflow rate of our prototype and its effectiveness, which provided us guidance on how to design our prototype. We compared the modeled results with experimental data  to asses the reliability of our model. With numerous factors considered from the dissolution capacity of carbon dioxide in water, the enzymatic reaction rate of CA to photosynthetic rate of cyanobacteria, our model is comprehensive and serves as an accurate prediction for the experimental results. Through modeling, we were able to estimate the effectiveness and limitations of our prototype accurately.</li>
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          <a href="https://2018.igem.org/Team:USTC/Notebook">NOTEBOOK</a>
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        <!--Notebook-->
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<li>We believe our engineered system does work under realistic conditions.</li>
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          <a href="#HPSubmenu" data-toggle="collapse" aria-expanded="false">HUMAN PRACTICES <i class="fa fa-caret-down"></i></a>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices">Overview</a>
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<a href="https://2019.igem.org/Team:Mingdao/Public_Engagement"><h2>Education and Public Engagement</h2></a>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices/General_HP">General HP</a>
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<ul>
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<li>We introduced synthetic biology and our project to the public, raising awareness of the danger of terrible indoor air quality. We taught younger students some simple knowledges and intrigued their interest in gene editing and the pros and cons about GMOs. We also held an one-day camp to teach them how to use basic equipment to operate easy experiments. For raising public awareness, we communicated to the voice club where students can share different ideas and discuss with others ,making our project more complete. Another took place in our own class, we asked them about their feelings both in hign CO2 concentration and low CO2 concentration , and the effect was apparent. Through surveys, we found that a lot of people were quite interested in our project and willing to give us advice to make our project better. They really encouraged us.</li>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices/Integrated_HP">Integrated HP</a>
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      <a href="https://2018.igem.org/Team:USTC/Collaborations">Collaborations</a>
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<!--Achievements-->
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          <a href="https://2018.igem.org/Team:USTC/Achievements">ACHIEVEMENTS</a>
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          <a href="#Teamsubmenu" data-toggle="collapse" aria-expanded="false">TEAM <i class="fa fa-caret-down"></i></a>
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              <a href="https://2018.igem.org/Team:USTC/Team">Members</a>
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              <a href="https://2018.igem.org/Team:USTC/Attributions">Attributions</a>
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<a href="https://2019.igem.org/Team:Mingdao/Human_Practices"><h2>Integrated Human Practices</h2></a>
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<ul>
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<li>We visited lots of professional authorities from different fields, all of them gave us countless useful comments. We put those suggestions into practice and improve our project, finally  sharing our knowledges with other teams as well. For our project design,we visited the department of environmental engineering in NCHU and a algae specialist, learning the current ways to solve the CO2 problem and the potential of the algae. For prototype design, we visited plenty of associations and companies.We learned the juncture of the indoor CO2 problems and the standard of normal air quality, which is the goal we want to achieve. Whatmore, we learned more knowledges about the algae from VEDAN, and discussed our prototype with some professors. At last, through the discussions and visits, we developed our prototype ,sharing our experience of human practice, how we produce our product, and the details of our design with the other teams.</li>
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<a href="https://2019.igem.org/Team:Mingdao/Hardware"><h2>Hardware</h2></a>
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<ul>
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<li>Air purifiers are unable to remove air pollutants such as CO2 and VOCs, etc. Our goal is to solve this problem to make indoor air fresh again. In product design, we created the high-performance and outstanding devices and APP to test our product and algae purification system.  CAir™ is the most convenient and portable photobioreactor with LED light, air pump, bubble disk and electric supply. CAir Pro™ is the perfect indoor air purifier equipped with extra CO2/O2 sensors, fan, blender and Arduino Nano board with Bluetooth. Users can install Mobile APP (iGEM Mingdao CAir Pro™) to wirelessly control the switch of LED, the pumps, the speed of the blender rotation, and the speed of the fan. We hope everyone enjoy our prototype and design! </li>
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</ul>
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<div id="medalBox5">
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<a href="https://2019.igem.org/Team:Mingdao/Model"><h2>Model</h2></a>
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<ul>
 +
<li>The model we developed was impactful on the design of our experiment and prototype. Our model demonstrated the effectiveness of the human Carbonic Anhydrase (CA) II enzyme in dissolving carbon dioxide in water and reducing the indoor carbon dioxide concentration, helping to prove the feasibility of our project design during initial testing prior to experiments. A mathematical correlation was found between the inflow rate of our prototype and its effectiveness, which provided us guidance on how to design our prototype. We compared the modeled results with experimental data  to asses the reliability of our model. With numerous factors considered from the dissolution capacity of carbon dioxide in water, the enzymatic reaction rate of CA to photosynthetic rate of cyanobacteria, our model is comprehensive and serves as an accurate prediction for the experimental results. Through modeling, we were able to estimate the effectiveness and limitations of our prototype accurately. </li>
 +
</ul>
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</div>
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<div id="medalBox5">
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<a href="https://2019.igem.org/Team:Mingdao/Part_Collection"><h2>Part Awards</h2></a>
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<ul>
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<li>Indoor air pollutants are a serious problem, and CAirTM is one of the best solutions to it. To produce enzymes that can be applied to CAirTM, we developed a CAirTM kit to do so.
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We collected DNA materials that can be used to produce proteins that can help algae purify the air for CAirTM. Such proteins as CA and CYP2E1 can be produced with this kit. Combining CAirTM and CAirTM kit, everyone is able to purify the air by themselves.
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To make materials in the CAirTM kit easy to use, we designed a GFP reporter under PliaI promoter. The protein induction and expression level can be tested with this plasmid.  </li>
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</ul>
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</div>
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</body>
  
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                                    <td scope="row">1</td>
 
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                                    <td>Registration and Jamboree Attendance</td>
 
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                                    <td>We tried our best to realize our project during the summer holiday with great enthusiasm. We also come to attend the Giant Jamboree, sharing our project and learning from other teams’ great ideas!</td>
 
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                                    <td>Competition Deliverables</td>
 
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                                    <td>We have filled out all the forms and pages that are required carefully. Also, we prepared our poster and presentation to show our project to everyone else.</td>
 
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                                    <td>Attributions</td>
 
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                                    <td>Here is the link for attributions: <a class="card-link" href="https://2018.igem.org/Team:USTC/Attributions">https://2018.igem.org/Team:USTC/Attributions</a>. We sincerely thank those people whom had given so much support to us.</td>
 
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                                    <td>Characterization/Contribution</td>
 
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                                    <td>We successfully participated in the 2018 InterLab Study and our result had been accepted by iGEM Foundation. Here is the link: <a class="card-link" href="https://2018.igem.org/Team:USTC/Attributions">https://2018.igem.org/Team:USTC/Interlab</a></td>
 
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                        <h4 class="card-title">Silver:</h4>
 
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                                    <td>Validated Part</td>
 
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                                    <td>This year we use quorum sensing genes from distribution kit to construct JTPI (BBa_K2768010), which can activate expression of genes downstream. And we also added a positive feedback to make it able to regulate in low AHL concentration. We have proved that this part can activate the expression downstream with positive feedback. You can check it here: <a class="card-link" href="http://parts.igem.org/Part:BBa_K2768010">http://parts.igem.org/Part:BBa_K2768010</a></td>
 
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                                    <td>Collaboration</td>
 
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                                    <td>We communicated with Team: Fudan, Nanjing-China, XJTU-China, Jilin-China, BNU-China about our projects, and we provided Team: HUST_China with experimental materials they needed. We helped Team: USTC-Software to test their Software and gave them some useful advice. Team Peking helped us in our model. We participated in The 5th Conference of China iGEMer Community and The 6th iGEM Asia-Parcific Conference to show our project and communicate with other teams.</td>
 
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                                    <td>Human Practice</td>
 
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                                    <td>(1) We have been to three tobacco factories (Hefei Cigarette Factory, Zhangzhou Cigarette Factory and Wuhu Cigarette Factory) to research our project. We knew a lot about tobacco and tobacco waste, and we had a fresh understanding of pathway of producing the prodrug. (2) For the purpose of collecting the community’s views about tobacco and propagating iGEM synthetic biology, we designed a questionnaire and have collected hundreds of results. (3) In the “Science Open Weekend”, we opened the lab for thousands of people to visit. (4) We finished a lecture in the high school to publicize iGEM and synthetic biology knowledge. 5. We actively communicated with local high schools and gave them help of founding new iGEM high school teams. 6. We attended the Asia-Pacific iGEM Conference and Central China iGEM Consortium with iGEMers all over the world. 7. Communication and cooperation with some iGEM teams.</td>
 
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                        <h4 class="card-title">Gold:</h4>
 
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                                    <td>During the visit to the 3 tobacco factories, we have learnt a lot about tobacco industry. We considered to use pure nicotine or nicotine in environment to produce 3-succionylpyridine before, but after the visit, we decided to use tobacco waste as our raw material. Our human practices helped us a lot in our project, making our project more close to real industrial production, and we have designed our whole system based on our human practices.</td>
 
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                                    <td>Improve a Previous Part</td>
 
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                                    <td>We added SsrA tag on the C terminus of Part: E0040, making it a new GFP with fast degradation rate (K2768004)</td>
 
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                                    <td>Model Your Project</td>
 
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                                    <td>(1) Single-cell model is consisted of 22 nonlinear ordinary differential equations. We use Matlab solvers to simulate the change of species concentration along the time. Then we analyze the condition of initial steady state and nicotine sensing state with defined parameters. These results strongly prove the feasibility of our design. (2) We analyze the impact of different combinations of promoter strengths and copy numbers to the initial steady states and outputs with nicotine input. Our simulation results present the tendency of initial state change and the signal shifting. According to these phenomena, we can choose the appropriate promoter combinations and plasmid backbones for the realization of our design. (3) In simulation, we change the value of promoter strength and nicotine input. From the simulation results, we acquire the best time for sampling, signal measurement and product collection. At last, the recycle rate (production rate) is calculated to prove the efficiency of manufacturing.</td>
 
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                                    <td>Demonstration of Your Work</td>
 
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                                    <td>We completed most of our design this year. In Sensing System, we selected the most suitable RBS and promotor for our sensing system under the guidance of modeling groups. We successfully constructed the plasmids and confirmed the transformation. In Regulation System, we constructed the plasmids we needed and plotted RFI curve and growth curve. According to the curves, we have proved our Regulation System is worked. In Degradation System, we constructed plasmids containing the genes of three enzymes separately and together. After confirming transformation, we successfully expressed three enzymes separately and together. In summary, it can be safely concluded that our project work! </td>
 
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{{:Team:Mingdao/test9}}

Latest revision as of 00:57, 22 October 2019

HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Notebook
Contribution
Results
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM ⇗
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Competition Deliverables

  • All deliverables were met.

Attributions
  • You can check out what our team did and how others helped our project.

Project Inspiration and Description
  • We introduce what inspires us and what we have done on our project. Check out on our Description Page

Parts
  • We submitted quantitative experimental characterization data to an existing Part from the Registry of Standard Biological Parts .
  • Validation can be found on our result page and registry.

Human Practices
  • We have investigated related issues and engaged with our relevant communities.

Collaboration
  • We have significantly worked with currently registered 2019 iGEM teams in a meaningful way.

Integrated Human Practices
  • We expanded on our silver medal activity by demonstrating how we have integrated the investigated issues into our project. Integrated human practices helped to improve our design and execution of the project.

Model Our Project
  • The model we developed was impactful on the design of our experiment and prototype. Our model demonstrated the effectiveness of the human Carbonic Anhydrase (CA) II enzyme in dissolving carbon dioxide in water and reducing the indoor carbon dioxide concentration, helping to prove the feasibility of our project design during initial testing prior to experiments. A mathematical correlation was found between the inflow rate of our prototype and its effectiveness, which provided us guidance on how to design our prototype. We compared the modeled results with experimental data to asses the reliability of our model. With numerous factors considered from the dissolution capacity of carbon dioxide in water, the enzymatic reaction rate of CA to photosynthetic rate of cyanobacteria, our model is comprehensive and serves as an accurate prediction for the experimental results. Through modeling, we were able to estimate the effectiveness and limitations of our prototype accurately.

Demonstration of Our Work
  • We believe our engineered system does work under realistic conditions.

Education and Public Engagement
  • We introduced synthetic biology and our project to the public, raising awareness of the danger of terrible indoor air quality. We taught younger students some simple knowledges and intrigued their interest in gene editing and the pros and cons about GMOs. We also held an one-day camp to teach them how to use basic equipment to operate easy experiments. For raising public awareness, we communicated to the voice club where students can share different ideas and discuss with others ,making our project more complete. Another took place in our own class, we asked them about their feelings both in hign CO2 concentration and low CO2 concentration , and the effect was apparent. Through surveys, we found that a lot of people were quite interested in our project and willing to give us advice to make our project better. They really encouraged us.

Integrated Human Practices
  • We visited lots of professional authorities from different fields, all of them gave us countless useful comments. We put those suggestions into practice and improve our project, finally sharing our knowledges with other teams as well. For our project design,we visited the department of environmental engineering in NCHU and a algae specialist, learning the current ways to solve the CO2 problem and the potential of the algae. For prototype design, we visited plenty of associations and companies.We learned the juncture of the indoor CO2 problems and the standard of normal air quality, which is the goal we want to achieve. Whatmore, we learned more knowledges about the algae from VEDAN, and discussed our prototype with some professors. At last, through the discussions and visits, we developed our prototype ,sharing our experience of human practice, how we produce our product, and the details of our design with the other teams.

Hardware
  • Air purifiers are unable to remove air pollutants such as CO2 and VOCs, etc. Our goal is to solve this problem to make indoor air fresh again. In product design, we created the high-performance and outstanding devices and APP to test our product and algae purification system. CAir™ is the most convenient and portable photobioreactor with LED light, air pump, bubble disk and electric supply. CAir Pro™ is the perfect indoor air purifier equipped with extra CO2/O2 sensors, fan, blender and Arduino Nano board with Bluetooth. Users can install Mobile APP (iGEM Mingdao CAir Pro™) to wirelessly control the switch of LED, the pumps, the speed of the blender rotation, and the speed of the fan. We hope everyone enjoy our prototype and design!

Model
  • The model we developed was impactful on the design of our experiment and prototype. Our model demonstrated the effectiveness of the human Carbonic Anhydrase (CA) II enzyme in dissolving carbon dioxide in water and reducing the indoor carbon dioxide concentration, helping to prove the feasibility of our project design during initial testing prior to experiments. A mathematical correlation was found between the inflow rate of our prototype and its effectiveness, which provided us guidance on how to design our prototype. We compared the modeled results with experimental data to asses the reliability of our model. With numerous factors considered from the dissolution capacity of carbon dioxide in water, the enzymatic reaction rate of CA to photosynthetic rate of cyanobacteria, our model is comprehensive and serves as an accurate prediction for the experimental results. Through modeling, we were able to estimate the effectiveness and limitations of our prototype accurately.

Part Awards
  • Indoor air pollutants are a serious problem, and CAirTM is one of the best solutions to it. To produce enzymes that can be applied to CAirTM, we developed a CAirTM kit to do so. We collected DNA materials that can be used to produce proteins that can help algae purify the air for CAirTM. Such proteins as CA and CYP2E1 can be produced with this kit. Combining CAirTM and CAirTM kit, everyone is able to purify the air by themselves. To make materials in the CAirTM kit easy to use, we designed a GFP reporter under PliaI promoter. The protein induction and expression level can be tested with this plasmid.
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