Difference between revisions of "Team:Cornell/Collaborations"

 
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{{Cornell}}
 
 
<html>
 
<html>
  
 +
<head>
 +
  <style>
 +
    /* hides "Team:Cornell" and iGEM logo */
 +
    #top_title {
 +
      display: none;
 +
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 +
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 +
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 +
      margin-left: 0px;
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−
<div class="clear"></div>
+
    /* removes default styling for home banner tagline */
 +
    #HQ_page p {
 +
      font-family: 'Open Sans', sans-serif;
 +
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 +
    /* removes small extra margin at very bottom below footer */
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    p {
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      margin: 0;
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<div class="column full_size">
+
    /* removes bullets from Toolkit dropdown menu */
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<h1>Collaborations</h1>
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    ul {
 +
      list-style-image: none;
 +
    }
  
−
<p>
+
    /******************** STANDARD PAGE GRID START ********************/
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
+
    nav {
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</p>
+
        grid-area: navbar;
 +
    }
  
−
<h3>Silver Medal Criterion #2</h3>
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    .nav-bar-main-menu .dropdown:nth-child(5) .dropdown-contentx {
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<p>
+
        display: grid;
−
Document your collaboration(s) clearly on this page to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2019.igem.org/Judging/Medals">2019 Medals Page</a> for more information.
+
        grid-template-columns: auto auto auto;
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</p>
+
    }
−
</div>
+
  
−
<div class="column two_thirds_size">
+
    .nav-first-col {
 +
        display: inline-grid;
 +
    }
  
−
<h4> Which other teams can we work with? </h4>
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    .nav-second-col {
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<p>
+
        display: inline-grid;
−
You can work with any other team in the competition, including standard, software, open, and high school track teams. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
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    }
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</p>
+
  
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<p>
+
    .nav-third-col {
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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        display: inline-grid;
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</p>
+
    }
  
−
</div>
+
    footer {
 +
        grid-area: footer;
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 +
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 +
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 +
    .standard-page-side-bar-content-wrapper {
 +
        display: inline-grid;
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        grid-area: standardpagesidebarcontent;
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        grid-template-columns: 7.5% 20% 65% 7.5%;
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    .standard-page-side-bar-wrapper {
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 +
    .standard-page-content-wrapper {
 +
        grid-area: standardpagecontent;
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 +
    /******************** STANDARD PAGE GRID END ********************/
  
−
<div class="column third_size">
+
    /******************** ALL FONTS START ********************/
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<p>
+
    @import url('https://fonts.googleapis.com/css?family=Merriweather|Open+Sans:400,700|Raleway');
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Here are some suggestions for projects you could work on with other teams:
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    @font-face {
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</p>
+
        font-family: 'Futura';
 +
        src: url('../futura/futura\ medium\ bt.ttf');
 +
    }
  
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<ul>
+
    body {
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<li> Improve the function of another team's BioBrick Part or Device</li>
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        font-family: 'Open Sans', sans-serif;
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<li> Characterize another team's part </li>
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    }
−
<li> Debug a construct </li>
+
−
<li> Model or simulate another team's system </li>
+
−
<li> Test another team's software</li>
+
−
<li> Help build and test another team's hardware project</li>
+
−
<li> Mentor a high-school team</li>
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</ul>
+
−
</div>
+
  
 +
    .dropbtn, .dropdown-content {
 +
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 +
        font-family: 'Futura', sans-serif;
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 +
    /******************** ALL FONTS END ********************/
 +
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    html,
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    body {
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        width: 100%;
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        padding: 0;
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    body {
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        background-size: 40px 40px;
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    p {
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        color: #000;
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    nav {
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        padding-top: 30px;
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        padding-bottom: 30px;
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        padding-right: 100px;
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        height: 98px;
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    .nav-bar-logo {
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        vertical-align: top;
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        display: inline-block;
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    .wet-lab-list-title,
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 +
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 +
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 +
 +
    footer {
 +
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 +
        position: relative;
 +
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 +
        background-color: #a0e0bd;
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 +
 +
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 +
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 +
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 +
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 +
 +
    .icon img {
 +
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 +
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 +
 +
    /******************** STANDARD PAGE CSS START ********************/
 +
 +
    .standard-page-banner {
 +
        background-size: cover;
 +
        fill: black;
 +
        font-size: 12px;
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 +
 +
    .standard-page-side-bar-content-wrapper {
 +
        padding: 5% 0;
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    .standard-page-side-bar>li>a {
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        font-size: 16px;
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    .standard-page-side-bar {
 +
        list-style-type: none;
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        padding-left: 15px;
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        padding-right: 15px;
 +
        vertical-align: middle;
 +
        margin: 40px !important;
 +
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 +
 +
    .standard-page-side-bar>li {
 +
        margin-bottom: 15px;
 +
    }
 +
 +
    .standard-page-content-title {
 +
        font-size: 24px;
 +
        color: #000 !important;
 +
        position: relative;
 +
    }
 +
 +
    .green-accent-line-left {
 +
        border: #a0e0bd solid 1px;
 +
        display: block;
 +
        float: left;
 +
        margin: 1em auto 1em auto;
 +
        width: 80px;
 +
    }
 +
 +
    .standard-page-content-subheading {
 +
        font-size: 18px;
 +
        font-weight: 1;
 +
        color: #000;
 +
        padding-top: 40px;
 +
        padding-bottom: 0;
 +
    }
 +
 +
    .standard-page-content-section {
 +
        margin-top: 40px;
 +
        margin-bottom: 40px;
 +
    }
 +
 +
    /******************** STANDARD PAGE CSS END ********************/
 +
  </style>
 +
  <title>Team:Cornell - 2019.igem.org</title>
 +
  <!-- CSS -->
 +
  <link rel="stylesheet" type="text/css" href="styles/styles.css">
 +
  <link rel="stylesheet" type="text/css" href="styles/grids.css">
 +
  <!-- JS -->
 +
  <script src="https://2019.igem.org/Team:Cornell/jquerymin?action=raw&ctype=text/javascript"></script>
 +
</head>
 +
<!--NOTE: REMEMBER TO CHANCE active-page CLASS TO CORRECT DROPDOWN-->
 +
 +
<body>
 +
  <div class="standard-page-wrapper">
 +
    <!------------------------ NAV BAR START ------------------------>
 +
    <nav>
 +
      <div class="nav-bar-wrapper">
 +
        <div class="nav-bar-logo">
 +
          <a href="https://2019.igem.org/Team:Cornell"><img src="https://static.igem.org/mediawiki/2019/9/94/T--Cornell--Logo.png" alt="reHAB"></a>
 +
        </div>
 +
        <div class="nav-bar-main-menu">
 +
          <div class="dropdown">
 +
            <button class="dropbtn">TEAM</button>
 +
            <div class="dropdown-content team-dropdown-content">
 +
              <a href="https://2019.igem.org/Team:Cornell/Team">BIOS</a>
 +
              <a href="https://2019.igem.org/Team:Cornell/Sponsors">SPONSORS</a>
 +
              <a href="https://2019.igem.org/Team:Cornell/Attributions">ATTRIBUTIONS</a>
 +
            </div>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn active-page">OUTREACH</button>
 +
            <div class="dropdown-content outreach-dropdown-content">
 +
              <a href="https://2019.igem.org/Team:Cornell/Public_Engagement">EDUCATION & ENGAGEMENT</a>
 +
              <a href="https://2019.igem.org/Team:Cornell/Collaborations">COLLABORATIONS</a>
 +
            </div>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn">HUMAN PRACTICES</button>
 +
            <div class="dropdown-content human-dropdown-content">
 +
              <a href="https://2019.igem.org/Team:Cornell/Human_Practices">INTEGRATED PRACTICES</a>
 +
              <a href="https://2019.igem.org/Team:Cornell/Policies">POLICIES</a>
 +
              <a href="https://2019.igem.org/Team:Cornell/Entrepreneurship">ENTREPRENEURSHIP</a>
 +
            </div>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn" onclick="location.href='https://2019.igem.org/Team:Cornell/Model'">MODELING</button>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn">TOOLKIT</button>
 +
            <div class="dropdown-content toolkit-dropdown-content">
 +
              <ul>
 +
                <div class="nav-first-col">
 +
                  <li class="wet-lab-list-title"><b>WET LAB</b></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Foundations">FOUNDATIONS</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Demonstrate">DEMONSTRATE</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Parts">PARTS</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/BasicParts">BASIC PARTS</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/CompositeParts">COMPOSITE PARTS</a></li>
 +
                </div>
 +
                <div class="nav-second-col">
 +
                  <li class="pd-list-title"><b>PRODUCT DEVELOPMENT</b></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/DesignProcess">DESIGN PROCESS</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Hardware">HARDWARE</a></li>
 +
                </div>
 +
                <div class="nav-third-col">
 +
                  <li class="doc-list-title"><b>DOCUMENTATION</b></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Notebook">NOTEBOOK</a></li>
 +
                  <li><a href="https://2019.igem.org/Team:Cornell/Safety">SAFETY</a></li>
 +
                </div>
 +
              </ul>
 +
            </div>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn"
 +
              onclick="location.href='https://2019.igem.org/Team:Cornell/Description'">ABOUT</button>
 +
          </div>
 +
          <div class="dropdown">
 +
            <button class="dropbtn"
 +
              onclick="location.href='https://2019.igem.org/Team:Cornell'">HOME</button>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </nav>
 +
    <!------------------------ NAV BAR END ------------------------>
 +
    <!------------------------ STANDARD PAGE BANNER START ------------------------>
 +
    <header class="standard-page-banner"
 +
      style="background-image: url(https://static.igem.org/mediawiki/2019/c/ce/T--Cornell--collab-banner.jpeg)">
 +
      <svg viewBox="0 0 100 100" width=100% height=100%>
 +
        <text text-anchor="middle" alignment-baseline="middle" x=50% y=50%>Collaborations</text>
 +
      </svg>
 +
    </header>
 +
    <!------------------------ STANDARD PAGE BANNER END ------------------------>
 +
    <!------------------------ STANDARD PAGE SIDE BAR + CONTENT START ------------------------>
 +
    <div class="standard-page-side-bar-content-wrapper">
 +
      <!------------------------ STANDARD PAGE SIDE BAR START ------------------------>
 +
      <div class="standard-page-side-bar-wrapper">
 +
        <ul class="standard-page-side-bar">
 +
          <li><a href="#pageheading1">Overview</a></li>
 +
          <li><a href="#pageheading2">Harmful Algal Blooms (HABs) Informational Booklet</a></li>
 +
          <li><a href="#pageheading3">Mentorship</a></li>
 +
          <li><a href="#pageheading4">William & Mary iGEM</a></li>
 +
          <li><a href="#pageheading5">SZTA_Szeged_HU iGEM</a></li>
 +
        </ul>
 +
      </div>
 +
      <!------------------------ STANDARD PAGE SIDE BAR END ------------------------>
 +
      <!------------------------ STANDARD PAGE CONTENT START ------------------------>
 +
      <div class="standard-page-content-wrapper">
 +
        <div class="standard-page-content-section">
 +
          <div class="standard-page-content-title">
 +
            <div id="pageheading1">Overview</div>
 +
          </div>
 +
          <hr class="green-accent-line-left">
 +
          <br> <br>
 +
          <p class="standard-page-content-text">
 +
            This year, Cornell iGEM had the honor of working with several different iGEM teams across the globe on both
 +
            technical- and outreach-oriented collaborations. We received valuable feedback on how to improve our
 +
            informational booklet designed to educate members of the community on the issue of harmful algal blooms
 +
            (HABs). We also mentored a new high school team and helped them get up-to-speed with how the iGEM
 +
            competition runs. Finally, we worked with another team to optimize and test components of the Wet Lab
 +
            aspects of our respective projects. Through these partnerships, our team was able to expand upon the
 +
            possibilities of collaborative synthetic biology and learn more about how we can solve big issues using
 +
            microscopic tools.
 +
          </p>
 +
        </div>
 +
        <div class="standard-page-content-section">
 +
          <div class="standard-page-content-title">
 +
            <div id="pageheading2">Harmful Algal Blooms (HABs) Informational Booklet</div>
 +
          </div>
 +
          <hr class="green-accent-line-left">
 +
          <br><br>
 +
          <p class="standard-page-content-text">
 +
            Cornell iGEM sought to demystify the subject of HABs by creating an informational booklet that summarizes
 +
            the background, prevalence, and existing treatment methods for HABs. We sent the first draft of our booklet
 +
            to Ohio State iGEM and Toronto iGEM to obtain feedback on the content and formatting of our booklet to
 +
            ensure it is readily accessible to readers. Below, we have detailed the feedback provided to us by these
 +
            teams and how we incorporated their suggestions into the final draft presented on our Wiki.
 +
          </p>
 +
          <div class="standard-page-content-subheading" style="font-weight: 1000">Ohio State iGEM</div>
 +
          <p class="standard-page-content-text">
 +
            The members of the Ohio State iGEM team found our booklet very informative and well-cited. However, their
 +
            feedback mentioned that our booklet had too much technical detail that occasionally took away from the
 +
            primary focus of each section, so we cut down and made our sections more concise. We also took their advice
 +
            to include a brief sentence explaining the examples listed on the Current Solutions page before discussing
 +
            their respective pros and cons.
 +
          </p>
 +
          <div class="standard-page-content-subheading" style="font-weight: 1000">Toronto iGEM</div>
 +
          <p class="standard-page-content-text">
 +
            Toronto iGEM recommended reformatting the Current Solutions page to make it easier for readers to compare
 +
            between example solutions and to recognize their respective strengths and weaknesses. Going off of this
 +
            suggestion, our team made modifications to the Current Solutions section and also decided to include an
 +
            additional page recapitulating the ways in which our project covers the weak points of other solutions.
 +
          </p>
 +
          <br><br>
 +
          <iframe src="https://static.igem.org/mediawiki/2019/b/ba/T--Cornell--Collabs.pdf" height="600" width="800"></iframe>
 +
          <br><br>
 +
        </div>
 +
        <div class="standard-page-content-section">
 +
          <div class="standard-page-content-title">
 +
            <div id="pageheading3">Mentorship</div>
 +
          </div>
 +
          <hr class="green-accent-line-left">
 +
          <div class="standard-page-content-subheading" style="font-weight: 1000">Korea_HS iGEM</div>
 +
          <p class="standard-page-content-text">
 +
            Over the summer, our team mentored the Korea_HS iGEM team after they reached out to us with questions about
 +
            the iGEM competition process. We held an initial Skype meeting on July 6th, 2019, where we shared our
 +
            project ideas and our experiences on iGEM. As it was Korea_HS’s first year participating in the competition,
 +
            we provided insight into what iGEM is and what Cornell iGEM has done as a team in the past. In regards to
 +
            the competition itself, Korea_HS had questions regarding the characterization section of the Bronze Medal
 +
            criteria and the formatting of the presentation and the poster, so we explained the characterization section
 +
            and provided tips on how to efficiently and effectively prepare for each portion of the competition. We had
 +
            a follow-up meeting on August 3rd, 2019, for an update on the team’s progress on their project and to answer
 +
            any remaining questions they had regarding the competition. </p>
 +
          <img class="standard-page-content-image" src="https://static.igem.org/mediawiki/2019/0/01/T--Cornell--Korea.png"
 +
            style="height: 400px; width: 650px">
 +
          <div class="standard-page-content-subheading" style="font-weight: 1000">Ohio State iGEM</div>
 +
          <p class="standard-page-content-text">
 +
            Our team also spoke with the Ohio State iGEM team to provide guidance on team organization and the competition timeline. We provided recommendations based on the resources that have worked best for our team, such as the online scheduling platform When2Meet that helps with finding meeting times that work best for the group. We also addressed questions Ohio State iGEM had regarding how to integrate human practices into their project. </p>
 +
        </div>
 +
        <div class="standard-page-content-section">
 +
          <div class="standard-page-content-title">
 +
            <div id="pageheading4">William & Mary iGEM</div>
 +
          </div>
 +
          <hr class="green-accent-line-left">
 +
          <br><br>
 +
          <p class="standard-page-content-text">
 +
            On September 22nd, Cornell iGEM held a Skype meeting with the members of W&M iGEM to discuss the integration of their curli fibers into our bioreactor. Since curli fibers enable biofilm development, our team sought to use them to encapsulate more bacteria, which would lead to higher efficiency. After we received the DNA parts from W&M iGEM, we compared the concentration of curli fiber-expressing bacteria and control bacteria in the beads. To do so, we cultured both types of bacteria overnight and induced the curli fiber expressing bacteria with 10 mM IPTG for 4 hours. We encapsulated both sets of bacteria using our alginate bead protocol and submerged them in water overnight. Then, 5 g of beads were dissolved in sodium citrate and serial dilutions were performed to find the final concentration of bacteria in our beads. We found that the control group had a colony count of roughly 4*10<sup>5</sup> whereas the modified groups yielded 2.5*10<sup>6</sup> colonies, so with a single run we were able to produce a significantly greater amount of bacteria. These results suggest that our system could be run at greater efficiency.
 +
            <br><br>
 +
            Our team also forwarded our alginate bead formation protocol to W&M iGEM, and they were able to successfully get their pDawn optogenetic bacteria into the beads. </p>
 +
          <img class="standard-page-content-image" src="https://static.igem.org/mediawiki/2019/d/de/T--Cornell--WilliamMary.png" style="height: 350px; width: 300px">
 +
          <img class="standard-page-content-image" src="https://static.igem.org/mediawiki/2019/8/84/T--Cornell--ControlGroup.png" style="height: 350px; width: 300px">
 +
          <img class="standard-page-content-image" src="https://static.igem.org/mediawiki/2019/a/a1/T--Cornell--TreatedGroup.png" style="height: 350px; width: 300px">
 +
        </div>
 +
        <div class="standard-page-content-section">
 +
          <div class="standard-page-content-title">
 +
            <div id="pageheading5">SZTA_Szeged_HU iGEM</div>
 +
          </div>
 +
          <hr class="green-accent-line-left">
 +
          <br><br>
 +
          <p class="standard-page-content-text">
 +
            Cornell iGEM held a Skype call with members of SZTA_Szeged_HU iGEM on September 14th. We exchanged public
 +
            engagement surveys as a way to broaden the scope of our respective projects by gaining feedback from an
 +
            international perspective.</p>
 +
        </div>
 +
      </div>
 +
      <!------------------------ STANDARD PAGE CONTENT END ------------------------>
 +
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          <a class="icon" href="http://twitter.com/cugem"><img
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          <a class="icon" href="https://www.instagram.com/cugem/" style="padding: 0;"><img
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 +
          <a class="icon" href="https://www.youtube.com/channel/UCJt-5JfyoucUJXC1EsTllhg"><img
 +
              src="https://static.igem.org/mediawiki/2019/3/3c/T--Cornell--Youtube.png"></a>
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Latest revision as of 21:41, 21 October 2019

Team:Cornell - 2019.igem.org

Collaborations
Overview


This year, Cornell iGEM had the honor of working with several different iGEM teams across the globe on both technical- and outreach-oriented collaborations. We received valuable feedback on how to improve our informational booklet designed to educate members of the community on the issue of harmful algal blooms (HABs). We also mentored a new high school team and helped them get up-to-speed with how the iGEM competition runs. Finally, we worked with another team to optimize and test components of the Wet Lab aspects of our respective projects. Through these partnerships, our team was able to expand upon the possibilities of collaborative synthetic biology and learn more about how we can solve big issues using microscopic tools.

Harmful Algal Blooms (HABs) Informational Booklet


Cornell iGEM sought to demystify the subject of HABs by creating an informational booklet that summarizes the background, prevalence, and existing treatment methods for HABs. We sent the first draft of our booklet to Ohio State iGEM and Toronto iGEM to obtain feedback on the content and formatting of our booklet to ensure it is readily accessible to readers. Below, we have detailed the feedback provided to us by these teams and how we incorporated their suggestions into the final draft presented on our Wiki.

Ohio State iGEM

The members of the Ohio State iGEM team found our booklet very informative and well-cited. However, their feedback mentioned that our booklet had too much technical detail that occasionally took away from the primary focus of each section, so we cut down and made our sections more concise. We also took their advice to include a brief sentence explaining the examples listed on the Current Solutions page before discussing their respective pros and cons.

Toronto iGEM

Toronto iGEM recommended reformatting the Current Solutions page to make it easier for readers to compare between example solutions and to recognize their respective strengths and weaknesses. Going off of this suggestion, our team made modifications to the Current Solutions section and also decided to include an additional page recapitulating the ways in which our project covers the weak points of other solutions.





Mentorship
Korea_HS iGEM

Over the summer, our team mentored the Korea_HS iGEM team after they reached out to us with questions about the iGEM competition process. We held an initial Skype meeting on July 6th, 2019, where we shared our project ideas and our experiences on iGEM. As it was Korea_HS’s first year participating in the competition, we provided insight into what iGEM is and what Cornell iGEM has done as a team in the past. In regards to the competition itself, Korea_HS had questions regarding the characterization section of the Bronze Medal criteria and the formatting of the presentation and the poster, so we explained the characterization section and provided tips on how to efficiently and effectively prepare for each portion of the competition. We had a follow-up meeting on August 3rd, 2019, for an update on the team’s progress on their project and to answer any remaining questions they had regarding the competition.

Ohio State iGEM

Our team also spoke with the Ohio State iGEM team to provide guidance on team organization and the competition timeline. We provided recommendations based on the resources that have worked best for our team, such as the online scheduling platform When2Meet that helps with finding meeting times that work best for the group. We also addressed questions Ohio State iGEM had regarding how to integrate human practices into their project.

William & Mary iGEM


On September 22nd, Cornell iGEM held a Skype meeting with the members of W&M iGEM to discuss the integration of their curli fibers into our bioreactor. Since curli fibers enable biofilm development, our team sought to use them to encapsulate more bacteria, which would lead to higher efficiency. After we received the DNA parts from W&M iGEM, we compared the concentration of curli fiber-expressing bacteria and control bacteria in the beads. To do so, we cultured both types of bacteria overnight and induced the curli fiber expressing bacteria with 10 mM IPTG for 4 hours. We encapsulated both sets of bacteria using our alginate bead protocol and submerged them in water overnight. Then, 5 g of beads were dissolved in sodium citrate and serial dilutions were performed to find the final concentration of bacteria in our beads. We found that the control group had a colony count of roughly 4*105 whereas the modified groups yielded 2.5*106 colonies, so with a single run we were able to produce a significantly greater amount of bacteria. These results suggest that our system could be run at greater efficiency.

Our team also forwarded our alginate bead formation protocol to W&M iGEM, and they were able to successfully get their pDawn optogenetic bacteria into the beads.

SZTA_Szeged_HU iGEM


Cornell iGEM held a Skype call with members of SZTA_Szeged_HU iGEM on September 14th. We exchanged public engagement surveys as a way to broaden the scope of our respective projects by gaining feedback from an international perspective.