Difference between revisions of "Template:Jilin China/Colla C.js"

Revision as of 20:05, 21 October 2019

/**

* 
* @authors lhx 
* @date    2019-05-30 16:25:38
* @version 1.1
*/

var content_alpha = { prospect:{ title:"Collaboration" }, part: [{ title: "JiangnanU_China", para: [

{ type: "word", cont: "Jilin Meetup", class: "np2" }, { type: "word", cont: "Jilin Meetup, which was initiated by JiangnanU_China and us, has been successfully held from July 19(sup)th(suped) to July 20(sup)th(suped), 2019 in Jilin University. The meeting was attended by Jilin_China, JiangnanU_China, NEU_CHINA, NEFU_China and NJTech_China, during which reports were respectively given on the projects of this year by each team before the difficulties found during the experiment were deeply discussed and communicated. Additionally, we analyzed the changes of prize criteria. Through 2019 Jilin Meetup, not only the projects were communicated, but also more profound friendship was forged among the teams, which marked the success of 2019 Jilin Meetup. This was the first time for us to conduct a successful iGEM Jilin Meetup which promoted our friendship.", class: "np5" }, { type: "pic", maxClass:"max5", cont: [{ num: 1, adress: "", pre: 100, }

] }, { type: "pic", maxClass:"max9", cont: [{ num: 1, adress: "", pre: 100, }

] }, { type: "word", cont: "JiangnanU_China’s assistance on the measurement of expression level of the promoter.", class: "np2" }, { type: "word", cont: "We measured the relative intensity of the P(sub)59(subed) promoter to the Anderson Promoter Family, yet the results of fluorescence measurement often vary under different experiments. We wanted to check whether the results of fluorescence experiment are consistent with other groups under same protocol condition in different laboratories, thus we invited JiangnanU_China to help to measure these six parts.", class: "np5" }, { type: "word", cont: "Measuring elements including BBa_J23119, BBa_J23104, BBa_J23108, BBa_J23105, BBa_J23114 and P(sub)59(subed) promoters were sent to JiangnanU_China, who got similar results to ours through the measurement of fluorescence intensity at emission wavelength 528 nm under excitation wavelength 485 nm. About our measurement please click here.", class: "np5" }, { type: "pic", maxClass:"max9", cont: [{ num: 1, adress: "", pre: 100, }

] }, { type: "word", cont: "Figure 1. Relative fluorescence (fluorescence/OD(sub)600(subed)) intensity at emission wavelength 528 nm under excitation wavelength 485 nm of each promoter measured by (A) Jilin_China and (B) JiangnanU_China over time.", class: "np5" }, { type: "word", cont: "1.3 The test of anti-phage parts’ impact on the growth of (ita)E. coli(itaed)", class: "np2" }, { type: "word", cont: "This year, the project of the JiangnanU_China team is to construct bacteria that are resistant to phage. They want to apply this constructed (ita)E. coli(itaed) strain to industrial production in the future, so they need to verify experiment under same protocol condition in different laboratories, thus we helped the JiangnanU_China team to test whether their anti-phage efficacy has a significant impact on the growth of (ita)E. coli(itaed), such as whether (ita)E. coli(itaed) will use a large amount of energy to express the anti-phage parts, which greatly affects their own growth. And this data will be applied to their model with the grey relation analysis in order to select the anti-phage part that has the least impact on bacterial growth. As shown in Figure 2, these genes expression would not affect (ita)E. coli (itaed)growth.", class: "np5" }, { type: "pic", maxClass:"max5", cont: [{ num: 1, adress: "", pre: 100, }

] }, { type: "word", cont: "Figure 2. Growth curve. Five different constructs, pET-28a, -(ita)dnaE(itaed), -(ita)vgrG(itaed), -(ita)rzpD(itaed), -(ita)gntR(itaed), -(ita)yhjH(itaed), -(ita)nuoE(itaed), were transformed into (ita)E. coli(itaed) respectively. OD(sub)600(subed) was detected at indicated time.", class: "np5" }, { type: "div", class: ["colla"], id: "jiangnan", } ]

}, { title: "OUC-China", para: [

{ type: "word", cont: "Test of OUC-China’s modular riboswitch by Jilin_China", class: "np2" }, { type: "word", cont: "Riboswitch is one of the structural genetic regulatory elements which can directly regulates the expression of downstream genes by the reaction of small molecules. It has potential future application prospects in the fields of synthetic biology because it has various of ligands. However, the secondary structure of riboswitches is unstable, so riboswitches are unable to be used as a modular component in synthetic biology. In order to solve the problem of modularization of riboswitches, OUC-China proposed the theory of modular riboswitches. They use the \"Stabilizer\"to stabilize the structure of the riboswitch and use the \"Tunner\" to separate Stabilizer from GOI in the range of experimental errors. In order to verify whether the feasibility of the modular riboswitch constructed by OUC-China is useful or not, they need to express their sequence in different experimental environments to verify the stability of the structure. Thus, our team helped them test their modular riboswitch based on the materials and experimental methods offered by OUC-China.", class: "np5" }, { type: "word", cont: "We analyzed the data after 8 hours of incubation. We can see that the fluorescence/RFU values of the groups of 2-AP (ligand) added with different concentrations (0, 50 μM/mL, 150 μM/mL) are obvious different. Therefore, the experimental results show that their system can work normally in different laboratory environments. The system is feasible.", class: "np5" }, { type: "pic", maxClass:"max5", cont: [{ num: 1, adress: "", pre: 100, }

] }, { type: "word", cont: "Figure 3. Modular riboswitch verification. Atc and different concentrations of 2-AP were added to the culture medium. After incubation for 8 hours, fluorescence was detected. The result have been normalized by OD(sub)600(subed).", class: "np5" }, { type: "word", cont: "OUC-China provided us with DNA 500bp Marker", class: "np2" }, { type: "div", class: ["colla"], id: "ouc", }

]

}, { title: "NJTech_China", para: [

{ type: "word", cont: "NJTech_China provided us with low range protein ladder(3 kD)", class: "np2" }, { type: "word", cont: "The low range protein ladder(3 kD) helped our LL-37 experimental group successfully verify the expression of LL-37 protein.", class: "np5" }, { type: "word", cont: "Our team sincerely offered help and suggestion to NJTech_China", class: "np2" }, { type: "word", cont: "NJTech_China participated in iGEM for the first time so our team sincerely offered help to them. This year, NJTech_China hopes to express the cjBlue protein(BBa_K592011). Fortunately, this part was used by 2018 Jilin_China last year, so we sent the plasmid containing this part to NJTech_China, and shared the experience about plate culturing time, expression of host strains and other experimental notes, which helped them greatly.", class: "np5" }, { type: "word", cont: "In addition, we communicated about how to improve and express the part. NJTech_China also reminded us of the need to represent an old part in the bronze standard, and we also reminded them to construct parts that conform to the assembly standard of biobrick or IIS.", class: "np5" },

{ type: "div", class: ["colla"], id: "njtech", }

]

}, { title: "Tianjin", para: [

{ type: "word", cont: "Video promotion of the \"The world of Microorganizm\" video promotion", class: "np2" }, { type: "word", cont: "Tianjin initiated the project of \"The world of Microorganizm\", which is participated by eight teams to call on people to take an active part in the scientific popularization. We utilize the advantages and the familiar bacteria of each lab into the colorful depiction of the world of microorganizm for the public.", class: "np5" }, { type: "div",

id: "jluvideo", }, { type: "div", class: ["colla"], id: "tianjin", }

]

}

]

}