Difference between revisions of "Team:Georgia State/Protocols"

 
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                                     <li class="active"><a href="https://2019.igem.org/wiki/index.php?title=Team:Georgia_State">Home</a></li>
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                                     <li><a href="https://2019.igem.org/wiki/index.php?title=Team:Georgia_State">Home</a></li>
 
                                     <li><a href="../Team:Georgia_State/Team">Team</a>
 
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                                             <li><a href="../Team:Georgia_State/Culturing">- Culturing</a></li>
 
                                             <li><a href="../Team:Georgia_State/Culturing">- Culturing</a></li>
 
                                             <li><a href="../Team:Georgia_State/Notebook">- Notebook</a></li>
 
                                             <li><a href="../Team:Georgia_State/Notebook">- Notebook</a></li>
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                                             <li><a href="../Team:Georgia_State/Protocols">- Protocols</a></li>
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                                             <li class="active"><a href="../Team:Georgia_State/Protocols">- Protocols</a></li>
 
                                             <li><a href="../Team:Georgia_State/Results">- Results</a></li>
 
                                             <li><a href="../Team:Georgia_State/Results">- Results</a></li>
 
                                             <li><a href="../Team:Georgia_State/AlgaeObservations">- Algae Observations</a></li>
 
                                             <li><a href="../Team:Georgia_State/AlgaeObservations">- Algae Observations</a></li>
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     </section>
 
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<p>put in the flow charts and create links to pdfs of our protocols<p>
 
 
<div class="container">
 
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<div class="col-12 col-sm-6 col-lg-3">
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<h4>Transformation Protocols</h4>
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                    <div class="single-service-area bg-white mb-80 wow fadeInUp" data-wow-delay="200ms">
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<li><a href="https://static.igem.org/mediawiki/2019/a/a9/T--Georgia_State--atum_smicrotrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a></li>
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                        <h5>Transformation Protocols</h5>
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<p><li><a href="https://static.igem.org/mediawiki/2019/a/a9/T--Georgia_State--atum_smicrotrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/3/38/T--Georgia_State--smicroelectro.pdf">Symbiodinium microadriaticum Electroporation Transformations</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/3/38/T--Georgia_State--smicroelectro.pdf">Symbiodinium microadriaticum Electroporation Transformations</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li>   
 
<li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li>   
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</p>
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                    </div>
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<h4>Culturing Medias</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a></li>
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<div class="col-12 col-sm-6 col-lg-3">
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                    <div class="single-service-area bg-white mb-80 wow fadeInUp" data-wow-delay="200ms">
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                    <h5>Culturing Medias</h5>
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<p><li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li>  
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li> </p>
  
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<h4>Other Techniques</h4>
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<h5>Other Techniques</h5>
 
<li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li>
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                    </div>
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</div>
 
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<p>Transforming pCB302-gfp-MBD into E. coli<p>
 
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<img src="https://static.igem.org/mediawiki/2019/thumb/4/4d/T--Georgia_State--pcb302_work_flow_into_E._coli.png/510px-T--Georgia_State--pcb302_work_flow_into_E._coli.png"></img>
 
  
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<p>Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens<p>
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<img src="https://static.igem.org/mediawiki/2019/thumb/d/d9/T--Georgia_State--pcb302_work_flow_into_Agro.png/503px-T--Georgia_State--pcb302_work_flow_into_Agro.png"></img>
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<caption>Transforming pCB302-gfp-MBD into E. coli</caption>
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<img src="https://static.igem.org/mediawiki/2019/1/11/T--Georgia_State--pCB302_wf_ecoli.png"></img>
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</div>
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<div class="col-6 justify-content-center">
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<caption>Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens</caption>
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<img src="https://static.igem.org/mediawiki/2019/d/d2/T--Georgia_State--pCB302_wf_Agro.png"></img>
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</div>
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</div>
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<div class="row mt-30 justify-content-center">
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<div class="col-4 justify-content-center">
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<caption>Codon Optimized RFP</caption>
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<img src="https://static.igem.org/mediawiki/2019/e/ee/T--Georgia_State--rfp_work_flow.png"</img>
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<div class="col-4 justify-content-center">
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<caption>Dino GFP</caption>
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<img src="https://static.igem.org/mediawiki/2019/f/f8/T--Georgia_State--dinoIII_gfp_work_flow.png"</img>
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</div>
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<div class="col-4 justify-content-center">
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<caption>Dino RFP</caption>
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<img src="https://static.igem.org/mediawiki/2019/2/2e/T--Georgia_State--dinoIII_rfp_work_flow.png"></img>
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</div>
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</div>
 
</div>
 
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<p>Codon Optimized RFP<p>
 
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<p>Dino GFP<p>
 
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<p>Dino RFP<p>
 
  
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</section>
  
 
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Latest revision as of 19:38, 21 October 2019

GSU iGEM

Protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli

    • Culturing Medias

  • ASP-8A Media
  • F2 Media
  • L1 Media

    • Other Techniques
  • Restriction Digest/Heat Kill
  • PCR
  • Overnight Cultures and Glycerol Stocks
  • Plasmid Extraction Mini Prep
  • Plasmid Extraction Midi Prep
  • Ligation
  • Gel Extraction
  • Gel Electrophoresis
  • Ethanol Precipitate
  • Lab Prep/Recipes
    • Transforming pCB302-gfp-MBD into E. coli
    Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens
    Codon Optimized RFP
    Dino GFP
    Dino RFP

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