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− | <a class="nav-brand" href=".. | + | <a class="nav-brand" href="../Team:Georgia_State"><img src="/wiki/images/2/23/T--Georgia_State--logo.png" alt=""></a> |
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<div class="classynav"> | <div class="classynav"> | ||
<ul id="nav"> | <ul id="nav"> | ||
− | <li | + | <li><a href="https://2019.igem.org/wiki/index.php?title=Team:Georgia_State">Home</a></li> |
<li><a href="../Team:Georgia_State/Team">Team</a> | <li><a href="../Team:Georgia_State/Team">Team</a> | ||
<ul class="dropdown"> | <ul class="dropdown"> | ||
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<li><a href="../Team:Georgia_State/Description">- Description</a></li> | <li><a href="../Team:Georgia_State/Description">- Description</a></li> | ||
<li><a href="../Team:Georgia_State/Design">- Design</a></li> | <li><a href="../Team:Georgia_State/Design">- Design</a></li> | ||
+ | <li><a href="../Team:Georgia_State/Culturing">- Culturing</a></li> | ||
<li><a href="../Team:Georgia_State/Notebook">- Notebook</a></li> | <li><a href="../Team:Georgia_State/Notebook">- Notebook</a></li> | ||
− | <li><a href="../Team:Georgia_State/Protocols">- Protocols</a></li> | + | <li class="active"><a href="../Team:Georgia_State/Protocols">- Protocols</a></li> |
<li><a href="../Team:Georgia_State/Results">- Results</a></li> | <li><a href="../Team:Georgia_State/Results">- Results</a></li> | ||
+ | <li><a href="../Team:Georgia_State/AlgaeObservations">- Algae Observations</a></li> | ||
<li><a href="../Team:Georgia_State/Hardware">- Hardware</a></li> | <li><a href="../Team:Georgia_State/Hardware">- Hardware</a></li> | ||
<li><a href="../Team:Georgia_State/Model">- Model</a></li> | <li><a href="../Team:Georgia_State/Model">- Model</a></li> | ||
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</ul></li> | </ul></li> | ||
<li><a href="../Team:Georgia_State/Safety">Safety</a></li> | <li><a href="../Team:Georgia_State/Safety">Safety</a></li> | ||
− | <li><a href=" | + | <li><a href="https://igem.org/2019_Judging_Form?team=Georgia_State">Judging Form</a></li> |
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</header> | </header> | ||
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+ | <!-- Breadcrumb Area Start --> | ||
+ | <section class="breadcrumb-area section-padding-80"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-12"> | ||
+ | <div class="breadcrumb-content"> | ||
+ | <h2>Protocols</h2> | ||
+ | <nav aria-label="breadcrumb"> | ||
+ | <ol class="breadcrumb"> | ||
+ | <li class="breadcrumb-item"><a href="../Team:Georgia_State"><i class="icon_house_alt"></i> Home</a></li> | ||
− | Transforming | + | <li class="breadcrumb-item active" aria-current="page">Protocols</li> |
− | < | + | </ol> |
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | <!-- Breadcrumb Area End --> | ||
+ | <div class="container"> | ||
+ | <div class="row justify-content-center"> | ||
+ | <div class="col-12 col-sm-6 col-lg-3"> | ||
+ | <div class="single-service-area bg-white mb-80 wow fadeInUp" data-wow-delay="200ms"> | ||
+ | <h5>Transformation Protocols</h5> | ||
+ | <p><li><a href="https://static.igem.org/mediawiki/2019/a/a9/T--Georgia_State--atum_smicrotrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/3/38/T--Georgia_State--smicroelectro.pdf">Symbiodinium microadriaticum Electroporation Transformations</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-12 col-sm-6 col-lg-3"> | ||
+ | <div class="single-service-area bg-white mb-80 wow fadeInUp" data-wow-delay="200ms"> | ||
+ | <h5>Culturing Medias</h5> | ||
+ | <p><li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li> </p> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-12 col-sm-6 col-lg-3"> | ||
+ | <div class="single-service-area bg-white mb-80 wow fadeInUp" data-wow-delay="200ms"> | ||
+ | <h5>Other Techniques</h5> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/5/51/T--Georgia_State--rd.pdf">Restriction Digest/Heat Kill</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/9/9c/T--Georgia_State--pcr.pdf">PCR</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/f/f0/T--Georgia_State--ovgs.pdf">Overnight Cultures and Glycerol Stocks</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/8/8a/T--Georgia_State--miniprep.pdf">Plasmid Extraction Mini Prep</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/8/82/T--Georgia_State--midiprep.pdf">Plasmid Extraction Midi Prep</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/b/bf/T--Georgia_State--ligation.pdf">Ligation</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/2/22/T--Georgia_State--gel_extraction.pdf">Gel Extraction</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/f/f3/T--Georgia_State--gel_electrophoresis.pdf">Gel Electrophoresis</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/0/0c/T--Georgia_State--etoh.pdf">Ethanol Precipitate</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2019/0/05/T--Georgia_State--labprep.pdf">Lab Prep/Recipes</a></li> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
− | Transforming | + | <section class="section-padding-80 bg-gray"> |
+ | <div class="container"> | ||
+ | <div class="row justify-content-center"> | ||
+ | <div class="col-6 justify-content-center"> | ||
+ | <caption>Transforming pCB302-gfp-MBD into E. coli</caption> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/1/11/T--Georgia_State--pCB302_wf_ecoli.png"></img> | ||
+ | </div> | ||
+ | <div class="col-6 justify-content-center"> | ||
+ | <caption>Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens</caption> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/d/d2/T--Georgia_State--pCB302_wf_Agro.png"></img> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row mt-30 justify-content-center"> | ||
+ | <div class="col-4 justify-content-center"> | ||
+ | <caption>Codon Optimized RFP</caption> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/e/ee/T--Georgia_State--rfp_work_flow.png"</img> | ||
+ | </div> | ||
+ | <div class="col-4 justify-content-center"> | ||
+ | <caption>Dino GFP</caption> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/f/f8/T--Georgia_State--dinoIII_gfp_work_flow.png"</img> | ||
+ | </div> | ||
+ | <div class="col-4 justify-content-center"> | ||
+ | <caption>Dino RFP</caption> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/2e/T--Georgia_State--dinoIII_rfp_work_flow.png"></img> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
− | < | + | </div> |
+ | </section> | ||
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Latest revision as of 19:38, 21 October 2019
![](https://static.igem.org/mediawiki/2019/1/11/T--Georgia_State--pCB302_wf_ecoli.png)
![](https://static.igem.org/mediawiki/2019/d/d2/T--Georgia_State--pCB302_wf_Agro.png)
![](https://static.igem.org/mediawiki/2019/e/ee/T--Georgia_State--rfp_work_flow.png)
![](https://static.igem.org/mediawiki/2019/f/f8/T--Georgia_State--dinoIII_gfp_work_flow.png)
![](https://static.igem.org/mediawiki/2019/2/2e/T--Georgia_State--dinoIII_rfp_work_flow.png)