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| − | img.parts{
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| − | </style>
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| − | </head>
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| − | <body>
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| − | <div class="Banner container-fluid text-center mb-0 align-items-center ">
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| − |
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| − | <div class="display-2 mb-0">
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| − |
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| − | <br>
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| − | <br>
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| − | <br>
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| − | <img src = "https://static.igem.org/mediawiki/2019/7/78/T--TUDelft--PartsOverview_logo.png" alt="Parts Overview" style="width:60%;">
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="centerjustify80">
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| − |
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| − | <p>...</p>
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| − | <br>
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| − | <br>
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| − |
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| − | <ul class="accordion">
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| − | <li>
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| − | <a class="toggle" href="javascript:void(0);" ><b>Our Best Basic Part</b><span style="float:right;"><b>﹀</b></span></a>
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| − | <div class="inner">
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| − | <div class="centerjustify90">
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| − | <table id="tabletu">
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| − |
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| − | <tr>
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| − | <th>♥ </th>
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| − | <th> Name </th>
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| − | <th> Description</th>
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| − | </tr>
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| − | <tr>
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| − | <td> ♥ </td>
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| − | <td> <a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918000">BBa_K2918000</a> </td>
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| − | <td> Φ29 DNA polymerase (DNAP/p2) </td>
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| − | </tr>
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| − | </table>
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| − | <br>
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| − | <p> Hello
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| − | </p>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <li>
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| − | <a class="toggle" href="javascript:void(0);" ><b>Our Best Composite Part</b><span style="float:right;"><b>﹀</b></span></a>
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| − | <div class="inner">
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| − | <div class="centerjustify90">
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| − | <table id="tabletu">
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| − |
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| − | <tr>
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| − | <th>♥ </th>
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| − | <th> Name </th>
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| − | <th> Description</th>
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| − | <th> Designer</th>
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| − | </tr>
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| − | <tr>
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| − | <td> ♥ </td>
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| − | <td> BBa_K2918000 </td>
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| − | <td> blablablablablablablablablablablabla </td>
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| − | <td> TU Delft 2019 </td>
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| − | </tr>
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| − | </table>
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| − | </div>
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| − | </div>
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| − | </li>
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| − |
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| − | <li>
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| − | <a class="toggle" href="javascript:void(0);" ><b>Our Part Collection</b><span style="float:right;"><b>﹀</b></span></a>
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| − | <div class="inner">
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| − | <div class="centerjustify90">
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| − | <p>Engineering non-model organisms requires discovery of organism-specific parts or re-tuning of parts characterized in model organisms. Our part collection provides modular cloning (MoClo) (Webber et al, 2011) compatible parts that can be used for predictable expression of genes across different bacterial species.
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| − | <br>
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| − | <br>
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| − | Our collections provides essential parts to overcome the need to identify parts for different bacterial hosts and insulate the parts from variations associated with microbial context. Our toolkit offers essential tools for effortless engineering of a range of bacterial species.
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| − | </p>
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| − | <br>
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| − |
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/a/a0/T--TUDelft--oriparts.png" alt="ori">
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| − |
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| − | </div>
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| − | <div class="column right">
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| − | <p> Identification of origins of replication for different bacterial species is laborious or requires knowledge of host organism. Although broad host range plasmids can be used across different bacterial species, they behave unpredictably and are dependent on host machinery (Jain and Srivastava 2013). Our toolkit provides all the components (four proteins and two origins of replication) of the Phi29 replication machinery necessary to establish host independent (orthogonal) replication. The advantage of establishing orthogonal replication, is little or no knowledge of bacterial host is needed and higher control of the system can be obtained. </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/1/1b/T--TUDelft--promoterparts.png" alt="promoter">
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| − | </div>
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| − | <div class="column right">
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| − | <p> Non-model bacterial species have a limited collection of promoters (Yang, Liu et al. 2018) . Our toolkit provides T7 promoter variants of different strengths which in combination with a T7 RNA polymerase mixed forward loop (UBER,hyperlink to design page), can be used for gene expression across different bacterial species. In addition, the collection consists of a broad host range promoter (Pbhr) based on conserved regions of -10 region and -35 region sequences in <i>E.coli</i> and <i>B.subtilis</i> (Yang, Liu et al. 2018) .
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/c/c1/T--TUDelft--riboparts.png" alt="ribozyme">
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| − | </div>
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| − | <div class="column right">
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| − | <p> To achieve modularity and predictability of synthetic parts, insulation from their genetic context needs to be established. Sequences downstream of the transcription start site (TSS) of promoters end up in transcripts and are shown to significantly affect translational rates (Lou et al. 2012). Our toolkit consists of ribozymes which when included in 5’UTR sequences, due to their self cleavage properties, cleave any artifacts left behind in the transcripts upstream of the RBS.
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/a/a6/T--TUDelft--rbsparts.png" alt="RBS">
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| − |
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| − | </div>
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| − | <div class="column right">
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| − | <p> Our toolkit contains an RBS (universal RBS) predicted to work using <a href="http://www.denovodna.com"> RBS calculator 2.0 </a> across a range of bacterial species.
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/6/6f/T--TUDelft--codonusage.png" alt="CDS">
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| − |
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| − | </div>
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| − | <div class="column right">
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| − | <p> Codon usage across bacterial species varies. Our toolkit provides a cross-species <a href="2019.igem.org/Team:TUDelft/Model">codon harmonization tool</a>. The tool can be used to generate coding sequences with equal codon usage across a range of organisms.
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| − |
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/0/05/T--TUDelft--terminatorparts.png" alt="terminator">
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| − |
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| − | </div>
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| − | <div class="column right">
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| − | <p> Our toolkit consists of variants of T7 terminators. Use of these variants reduces chances of homologous recombination between transcriptional units.
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <br>
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| − | <div class="row">
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| − | <div class="column left">
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| − | <img class="parts" src="https://static.igem.org/mediawiki/2019/4/4e/T--TUDelft--taleblue.png" alt="TALE">
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| − |
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| − | </div>
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| − | <div class="column right">
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| − | <p> Behaviour of parts is influenced by microbial context dependent variations (eg. copy number, transcription rates and translation rates) and to overcome the need to re-characterize parts for different bacterial species, an incoherent feed forward loop (iFFL) can be implemented in a genetic circuit (Segall-Shapiro et al. 2018). To establish an iFFL genetic circuit, TALE (Transcriptional Activator Like Effector molecule) proteins can be used as repressors. Our toolkit contains TALE protein designed to target a specific DNA sequence. Furthermore, we engineered a series of promoters that can be targeted by the TALE protein.
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| − | </p>
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| − | </div>
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| − | </div>
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| − |
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| − | </div>
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| − | <h1 style="border-bottom:none!important;"><b>Part Collection</b></h1>
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| − | <div class="ex2">
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| − | <table id="Table_2" class="pgrouptable addborder tablesorter" style="width:100%;" cellpadding="0" cellspacing="0"><thead><tr><th>♥</th><th style="width: 7em;" class="header">Name</th><th colspan="" style="padding-right:7px;" class="header">Type</th><th colspan="" style="padding-right:7px;" class="header headerSortDown">Description</th><th colspan="" style="padding-right:7px;" class="header">Designer</th><th colspan="" style="padding-right:7px;" class="header">Length</th></tr></thead><tbody>
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| − | <!-- ROW -->
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| − | <!-- ROW -->
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| − | <!-- ROW -->
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53809"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918005">BBa_K2918005</a></td><td>Regulatory</td><td>0.1 T7 promoter </td><td width="100px">TUDelft2019</td><td align="right">23</td>
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53931"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918006">BBa_K2918006</a></td><td>Regulatory</td><td>0.5 T7 promoter</td><td width="100px">TUDelft2019</td><td align="right">23</td>
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| − |
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| − |
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a></td><td>Regulatory</td><td>0.5 T7sp1 promoter </td><td width="100px">TUDelft 2019</td><td align="right">41</td>
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a></td><td>5'UTR</td><td>BBa_B0032 RBS and RiboJ</td><td width="100px">TUDelft 2019</td><td align="right">94</td>
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a></td><td>5'UTR</td><td>BBa_B0032 RBS and SarJ</td><td width="100px">TUDelft 2019</td><td align="right">98</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918000">BBa_K2918000</a></td><td>Regulatory</td><td>Broad Host Range Promoter (P<sub>BHR</sub>)</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918011">BBa_K2918011</a></td><td>Regulatory</td><td>P<sub>BHR</sub>sp1</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
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| − |
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55155"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a></td><td>5'UTR</td><td>Universal RBS and RiboJ </td><td width="100px">TUDelft 2019</td><td align="right">85</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a></td><td>Ribozyme</td><td>Ribo J</td><td width="100px">TUDelft 2019</td><td align="right">75</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a></td><td>5'UTR</td><td>SarJ and Universal RBS</td><td width="100px">TUDelft 2019</td><td align="right">89</td>
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54352"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a></td><td>Regulatory</td><td>T7sp1</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54349"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a></td><td>Terminator</td><td>T7 terminator variant</td><td width="100px">TUDelft 2019</td><td align="right">48</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54076"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918008">BBa_K2918008</a></td><td>Coding</td><td>Transcription Activator like Effector protein (TALEsp1)</td><td width="100px">TUDelft2019</td><td align="right">2640</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a></td><td>RBS</td><td>Universal RBS</td><td width="100px">TUDelft2019</td><td align="right">10</td>
| |
| − |
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918062">BBa_K2918062</a></td><td>Composite</td><td>TALEsp1 stablized P<sub>BHR</sub> promoter</td><td width="100px">TUDelft2019</td><td align="right">10</td>
| |
| − |
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54193"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a></td><td>Coding</td><td>Φ29 DNA polymerase (DNAP/p2)</td><td width="100px">TUDelft2019</td><td align="right">1719</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53810"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918003">BBa_K2918003</a></td><td>Coding</td><td>Φ29 Double Strand Binding Protein (DSB/p6)</td><td width="100px">TUDelft2019</td><td align="right">315</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53814"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918002">BBa_K2918002</a></td><td>Coding</td><td>Φ29 Single Stranded Binding Protein (SSB/p5)</td><td width="100px">TUDelft2019</td><td align="right">375</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a></td><td>Coding</td><td>Φ29 Terminal Protein (TP/p3)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918033">BBa_K2918033</a></td><td>ORI</td><td>Φ29 Left origin of replication (Ori-L)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a></td><td>ORI</td><td>Φ29 Right origin of replication (Ori-R)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918039">BBa_K2918039</a></td><td>Ribozyme</td><td>SarJ</td><td width="100px">TUDelft2019</td><td align="right">801</td>
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| − |
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53857"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr></tbody></table>
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| − | <a class="toggle" href="javascript:void(0);" ><b>All parts</b><span style="float:right;"><b>﹀</b></span></a>
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| − | <table id="Table_4" class="pgrouptable addborder tablesorter" style="width: 100%;" cellpadding="0" cellspacing="0"><thead><tr><th>♥</th><th style="width: 7em;" class="header">Name</th><th colspan="" style="padding-right:7px;" class="header">Type</th><th colspan="" style="padding-right:7px;" class="header headerSortDown">Description</th><th colspan="" style="padding-right:7px;" class="header">Designer</th><th colspan="" style="padding-right:7px;" class="header">Length</th></tr></thead><tbody>
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| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53809"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918005">BBa_K2918005</a></td><td>Regulatory</td><td>0.1 T7 promoter </td><td width="100px">TUDelft2019</td><td align="right">23</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53931"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918006">BBa_K2918006</a></td><td>Regulatory</td><td>0.5 T7 promoter</td><td width="100px">TUDelft2019</td><td align="right">23</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918007">BBa_K2918007</a></td><td>Regulatory</td><td>Wild type T7 promoter </td><td width="100px">TUDelft2019</td><td align="right">23</td></td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53816"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918009">BBa_K2918009</a></td><td>Regulatory</td><td>0.5 T7 promoter variant with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918035">BBa_K2918035</a></td><td>RBS</td><td>BBa_B0032 RBS and RiboJ</td><td width="100px">TUDelft 2019</td><td align="right">94</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54374"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918013">BBa_K2918013</a></td><td>RBS</td><td>BBa_B0032 RBS and SarJ</td><td width="100px">TUDelft 2019</td><td align="right">98</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53815"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918034">BBa_K2918034</a></td><td>Regulatory</td><td>Broad host range promoter</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54383"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918011">BBa_K2918011</a></td><td>Regulatory</td><td>Broad host range promoter with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">56</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55140"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918037">BBa_K2918037</a></td><td>Coding</td><td>Cross-species harmonized GFP</td><td width="100px">TUDelft 2019</td><td align="right">678</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54375"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918061">BBa_K2918061</a></td><td>Other</td><td>RBS (B0032) adapted for modular cloning with Ribozymes</td><td width="100px">TUDelft 2019</td><td align="right">25</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55155"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918038">BBa_K2918038</a></td><td>RBS</td><td>RiboJ and universal binding site</td><td width="100px">TUDelft 2019</td><td align="right">85</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="55150"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918012">BBa_K2918012</a></td><td>Other</td><td>Ribozyme J (Ribo J)</td><td width="100px">TUDelft 2019</td><td align="right">75</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54379"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918036">BBa_K2918036</a></td><td>RBS</td><td>SarJ and universal ribosome binding site </td><td width="100px">TUDelft 2019</td><td align="right">89</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54352"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918010">BBa_K2918010</a></td><td>Regulatory</td><td>T7 promoter with TALEsp1 binding site</td><td width="100px">TUDelft 2019</td><td align="right">41</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54349"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918016">BBa_K2918016</a></td><td>Terminator</td><td>T7 terminator variant</td><td width="100px">TUDelft 2019</td><td align="right">48</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54076"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918008">BBa_K2918008</a></td><td>Coding</td><td>Transcription Activator like Effector protein (TALEsp1)</td><td width="100px">TUDelft2019</td><td align="right">2640</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54344"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918014">BBa_K2918014</a></td><td>RBS</td><td>Universal RBS</td><td width="100px">TUDelft2019</td><td align="right">10</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53929"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918062">BBa_K2918062</a></td><td>RBS</td><td>Universal RBS site adapted for modular cloning with ribozymes</td><td width="100px">TUDelft 2019</td><td align="right">16</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54257"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918015">BBa_K2918015</a></td><td>Terminator</td><td>Wild type T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">48</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54193"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918000">BBa_K2918000</a></td><td>Coding</td><td>Φ29 DNA polymerase (DNAP/p2)</td><td width="100px">TUDelft2019</td><td align="right">1719</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53810"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918003">BBa_K2918003</a></td><td>Coding</td><td>Φ29 Double Strand Binding Protein (DSB/p6)</td><td width="100px">TUDelft2019</td><td align="right">315</td>
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| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53814"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918002">BBa_K2918002</a></td><td>Coding</td><td>Φ29 Single Stranded Binding Protein (SSB/p5)</td><td width="100px">TUDelft2019</td><td align="right">375</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53812"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918001">BBa_K2918001</a></td><td>Coding</td><td>Φ29 Terminal Protein (TP/p3)</td><td width="100px">TUDelft2019</td><td align="right">801</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54076"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918017">BBa_K2918017</a></td><td>Composite</td><td>0.1 T7 promoter - Universal RBS - Φ29 9 TP - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">890</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54191"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918018">BBa_K2918018</a></td><td>Composite</td><td>0.5 T7 promoter - Universal RBS - Φ29 TP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">904</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54192"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td>♥</td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918019">BBa_K2918019</a></td><td>Composite</td><td>WT T7 promoter - Universal RBS - Φ29 TP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">904</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54193"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918020">BBa_K2918020</a></td><td>Composite</td><td>0.1 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">1822</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54194"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918021">BBa_K2918021</a></td><td>Composite</td><td>0.5 T7 promoter - Universal RBS - Φ29 DNAP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">1822</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53926"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918022">BBa_K2918022</a></td><td>Composite</td><td>WT T7 promoter - Universal RBS - Φ29 DNAP - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">1899</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53813"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918023">BBa_K2918023</a></td><td>Composite</td><td>0.1 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">519</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53927"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918024">BBa_K2918024</a></td><td>Composite</td><td>0.5 T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">519</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53928"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918025">BBa_K2918025</a></td><td>Composite</td><td>WT T7 promoter - Universal RBS - Φ29 DSB (p6) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">519</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53930"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918026">BBa_K2918026</a></td><td>Composite</td><td>0.1 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">579</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53931"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918027">BBa_K2918027</a></td><td>Composite</td><td>0.5 T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">579</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="53932"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918028">BBa_K2918028</a></td><td>Composite</td><td>WT T7 promoter - Universal RBS - Φ29 SSB (p5) - WT T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">579</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54195"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918030">BBa_K2918030</a></td><td>Composite</td><td>WT T7 promoter - Universal RBS - GFP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">811</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54196"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918031">BBa_K2918031</a></td><td>Composite</td><td>0.1 T7 promoter - Universal RBS - GFP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">811</td>
| |
| − |
| |
| − | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2019&group=TUDelft" enctype="multipart/form-data"></form><input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="54198"><input type="hidden" name="pgroup" value="iGEM2019"><input type="hidden" name="group" value="TUDelft"></tr><tr><td></td><td><a target="_blank" class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2918032">BBa_K2918032</a></td><td>Composite</td><td>0.5 T7 promoter - Universal RBS - GFP - T7 terminator</td><td width="100px">TUDelft2019</td><td align="right">811</td></tr></tbody></table>
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| − | <h3>References</h3>
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| − | <ol>
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| − | <li>
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| − | <a id="RBS calculator" href="https://www.nature.com/articles/nbt.1568" target="_blank">
| |
| − | Salis, H. M., et al. (2009). "Automated design of synthetic ribosome binding sites to control protein expression." <u>Nature Biotechnology </u> 27(10): 946-950.
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| − | </a>
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| − | </li>
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| − | <li>
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| − | <a id="Segall-Shapiro" href="https://doi.org/10.1038/nbt.4111" target="_blank">
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| − | Segall-Shapiro, T. H., <i>et al.</i> (2018). "Engineered promoters enable constant gene expression at any copy number in bacteria." <u>Nature Biotechnology</u> 36: 352.
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| − | </a>
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| − | </li>
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| − | <li>
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| − | <a id="Yang" href="https://www.ncbi.nlm.nih.gov/pubmed/29061047" target="_blank">
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| − | Yang, S., et al. (2018). "Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology." <u> ACS Synthetic Biology </u> 7(1): 287-291.
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| − | </a>
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| − | </li>
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| − | <li>
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| − | <a id="Jain" href="https://academic.oup.com/femsle/article/348/2/87/731695" target="_blank">
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| − | Jain, A. and P. Srivastava (2013). "Broad host range plasmids." <u> FEMS Microbiology Letters </u>348(2): 87-96.
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| − | </a>
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