Difference between revisions of "Team:Georgia State/Protocols"

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<p>put in the flow charts and create links to pdfs of our protocols<p>
 
<p>put in the flow charts and create links to pdfs of our protocols<p>
<h4>Transformation Protocol</h4>
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<h4>Transformation Protocols</h4>
<li><a href="">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/a/a9/T--Georgia_State--atum_smicrotrans.pdf">Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a></li>
 
<li><a href="https://static.igem.org/mediawiki/2019/2/28/T--Georgia_State--Lonza_electro.pdf">4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum</a></li>
<li><a href="https://static.igem.org/mediawiki/2019/3/38/T--Georgia_State--smicroelectro.pdf">Symbiodinium microadriaticum Electroporation Transformations</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/3/38/T--Georgia_State--smicroelectro.pdf">Symbiodinium microadriaticum Electroporation Transformations</a></li>
<h4>Culturing Media</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a>></li>
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<li><a href="">F2 Media</a></li>
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<li><a href="">L1 Media</a></li>
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<li><a href="">Transforming E.coli</a></li>  
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<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
 
<li><a href="https://static.igem.org/mediawiki/2019/d/df/T--Georgia_State--atumetrans.pdf">Transforming A. tumefaciens</a></li>  
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<li><a href="https://static.igem.org/mediawiki/2019/5/59/T--Georgia_State--Ecolitransformations.pdf">Transforming E.coli</a></li> 
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<h4>Culturing Medias</h4>
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<li><a href="https://static.igem.org/mediawiki/2019/c/c5/T--Georgia_State--ASP-8A_media.pdf">ASP-8A Media</a>></li>
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<li><a href="https://static.igem.org/mediawiki/2019/8/81/T--Georgia_State--F2_media.pdf">F2 Media</a></li>
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<li><a href="https://static.igem.org/mediawiki/2019/3/3c/T--Georgia_State--L1_media.pdf">L1 Media</a></li>
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<p>Restriction Digest/Heat Kill<p>
 
<p>Restriction Digest/Heat Kill<p>
 
<p>PCR<p>
 
<p>PCR<p>

Revision as of 01:15, 21 October 2019

GSU iGEM

put in the flow charts and create links to pdfs of our protocols

Transformation Protocols

  • Agrobacterium tumefaciens Mediated Transformation of Symbiodinium microadriaticum
  • 4D-Nucleofector Lonza Electroporations for O. marina and S.microadriaticum
  • Symbiodinium microadriaticum Electroporation Transformations
  • Transforming A. tumefaciens
  • Transforming E.coli
  • Culturing Medias

  • ASP-8A Media>
  • F2 Media
  • L1 Media
  • Restriction Digest/Heat Kill

    PCR

    Overnight Cultures and Glycerol Stocks

    Plasmid Extraction Mini Prep

    Plasmid Extraction Midi Prep

    Ligation

    Gel Extraction

    Gel Electrophoresis

    Ethanol Precipitate

    Lab Prep/Recipes

    Transforming pCB302-gfp-MBD into Escherichia coli

    Transforming pCB302-gfp-MBD into Agrobacterium tumefaciens

    Codon Optimized RFP

    Dino GFP

    Dino RFP