To use BL as a substrate in a efficient way , we design a metabolic network involving organelle engineering. To do this we aim three pillar project.
Selection of synthetic metabolic pathway. This pathway converts vanillic acid, a LDIC, into muconic acid, a nylon precursor.
Organelle engineering. Protein engineering to encapsulate enzymes and enhance permeability of an bacterial microcompartment.
Genetic circuit design. Would provide slightly regulation and optimization variables, as BMC assembly, growth and dynamic metabolic control.
To select the pathway we take objectives that:1. the final product may be higher value added product that BL burning to produce energy.2. can be placed in the market 3.can be extracted by petroleum derivatives.