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Rabbit CYP2E1

Part: BBa_K2932003

Cytochrome P450 are family of enzyme containing heme and all of them use O2 and electrons to oxidize substrates(2). They plays an important role in human liver cells metabolism, it can also degrade benzene and chloroform which affect human a lot indoors. The following is the pathway that CYP2E1 breaks down the benzene:

It will produce phenol while CYP2E1 breaking down benzene, so it is a proof for benzene broke down by CYP2E1.(4-aminoantipyrine/potassium ferricyanide (lll) Emerson reaction) Both oxidative and reductive pathways of chloroform metabolism are described in the following. Carbon dioxide is the major metabolite of chloroform generated by the oxidative pathway of metabolism in vivo(4).

We got the sequence CYP2E1 in rabbit from KEGG-Oryctolagus cuniculus-CYP2E1, and it was optimized to bacillus subtilis by IDT-Codon Optimization Tool. Twist Bioscience synthesized it to us.

CYP2E1 efficiency measure

Introduction:

CYP2E1 has the ability to break down benzene, one of the reaction will produce phenol, which can be degraded by algae. To measure the efficiency of CYP2E1, we use “Emerson reagent” as our way to detect the decomposition of benzene.