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<p>We got the sequence CYP2E1 in rabbit from <a href="https://www.genome.jp/dbget-bin/www_bget?ocu:100342572" target="_blank" title="KEGG-Oryctolagus cuniculus-CYP2E1">KEGG-Oryctolagus cuniculus-CYP2E1</a>, and it was optimized to bacillus subtilis by <a href="https://sg.idtdna.com/site/account/login?returnurl=%2FCodonOpt" target="_blank" title="IDT-Codon Optimization Tool">IDT-Codon Optimization Tool</a>. Twist Bioscience synthesized it to us.</p> | <p>We got the sequence CYP2E1 in rabbit from <a href="https://www.genome.jp/dbget-bin/www_bget?ocu:100342572" target="_blank" title="KEGG-Oryctolagus cuniculus-CYP2E1">KEGG-Oryctolagus cuniculus-CYP2E1</a>, and it was optimized to bacillus subtilis by <a href="https://sg.idtdna.com/site/account/login?returnurl=%2FCodonOpt" target="_blank" title="IDT-Codon Optimization Tool">IDT-Codon Optimization Tool</a>. Twist Bioscience synthesized it to us.</p> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2019/9/9f/T--Mingdao--image72.png" alt="" style="max-width:800px;"></center> | ||
Revision as of 08:26, 18 October 2019
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Rabbit CYP2E1
Part: BBa_K2932003
Cytochrome P450 are family of enzyme containing heme and all of them use O2 and electrons to oxidize substrates(2).
They plays an important role in human liver cells metabolism, it can also degrade benzene and chloroform which affect human a lot indoors. The following is the pathway that CYP2E1 breaks down the benzene:
![](https://static.igem.org/mediawiki/2019/f/f2/T--Mingdao--image74.png)
It will produce phenol while CYP2E1 breaking down benzene, so it is a proof for benzene broke down by CYP2E1.(4-aminoantipyrine/potassium ferricyanide (lll) Emerson reaction) Both oxidative and reductive pathways of chloroform metabolism are described in the following. Carbon dioxide is the major metabolite of chloroform generated by the oxidative pathway of metabolism in vivo(4).
![](https://static.igem.org/mediawiki/2019/d/d4/T--Mingdao--image70.png)
We got the sequence CYP2E1 in rabbit from KEGG-Oryctolagus cuniculus-CYP2E1, and it was optimized to bacillus subtilis by IDT-Codon Optimization Tool. Twist Bioscience synthesized it to us.
![](https://static.igem.org/mediawiki/2019/9/9f/T--Mingdao--image72.png)