Difference between revisions of "Team:Mingdao/Basic Part"
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They plays an important role in human liver cells metabolism, it can also degrade benzene and chloroform which affect human a lot indoors. The following is the pathway that CYP2E1 breaks down the benzene: | They plays an important role in human liver cells metabolism, it can also degrade benzene and chloroform which affect human a lot indoors. The following is the pathway that CYP2E1 breaks down the benzene: | ||
<center><img src="https://static.igem.org/mediawiki/2019/f/f2/T--Mingdao--image74.png" alt="" style="max-width:800px;"></center> | <center><img src="https://static.igem.org/mediawiki/2019/f/f2/T--Mingdao--image74.png" alt="" style="max-width:800px;"></center> | ||
| + | <p>It will produce phenol while CYP2E1 breaking down benzene, so it is a proof for benzene broke down by CYP2E1.(4-aminoantipyrine/potassium ferricyanide (lll) Emerson reaction) | ||
| + | Both oxidative and reductive pathways of chloroform metabolism are described in the following. Carbon dioxide is the major metabolite of chloroform generated by the oxidative pathway of metabolism in vivo(4). | ||
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Revision as of 07:14, 18 October 2019
Best Basic parts
Motivation
Project Design
Prototype Development
Conclusion
Public Engagement
Biotech Workshop
Public Awareness
Conclusion
Best Basic parts
Rabbit CYP2E1
Part: BBa_K2932003
Cytochrome P450 are family of enzyme containing heme and all of them use O2 and electrons to oxidize substrates(2).
They plays an important role in human liver cells metabolism, it can also degrade benzene and chloroform which affect human a lot indoors. The following is the pathway that CYP2E1 breaks down the benzene:
It will produce phenol while CYP2E1 breaking down benzene, so it is a proof for benzene broke down by CYP2E1.(4-aminoantipyrine/potassium ferricyanide (lll) Emerson reaction) Both oxidative and reductive pathways of chloroform metabolism are described in the following. Carbon dioxide is the major metabolite of chloroform generated by the oxidative pathway of metabolism in vivo(4).