Difference between revisions of "Team:Georgia State/notebook"

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     <title>GSU iGEM</title>
 
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     </header>
 
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     <!-- Header Area End -->
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<!-- Notebook Area Start -->
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    <!--Calender starts here-->
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<div class="container" style="background-color:black">
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  <img src="girl.png" alt="Notebook" style="width:500px;height:500px;" class="center">
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<br>
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  <div class="row align-items-start">
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      <!--June-->
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    <div class="col">
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      <div class="container">
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  <!--<h4 style="color:white">June 2019</h4>-->
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  <table class="table" cellspacing="0" cellpadding="0" id="table1">
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    <tr id="title">
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      <th colspan="7" class="text-center" id="header">June 2019</th>
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    </tr>
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    <tr id="days">
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      <th style="color:red">S</th>
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      <th>M</th>
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      <th>T</th>
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      <th>W</th>
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      <th>T</th>
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      <th>F</th>
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      <th>S</th>
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    </tr>
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    <tbody class="body">
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      <tr id="trow">
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    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
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    <td id="date-pdf">1</td>
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      </tr>
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      <tr id="trow">
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    <td id="date-pdf">2</td>
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    <td id="date-pdf">3</td>
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    <td id="date-pdf">4</td>
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    <td id="date-pdf">5</td>
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    <td id="date-pdf">6</td>
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    <td id="date-pdf">7</td>
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    <td id="date-pdf">8</td>
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      </tr>
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      <tr id="trow">
 +
    <td id="date-pdf">9</td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061019.pdf"><span class="tool" data-tip="Extracted the plasmid, PCB302-gfp-MBD, off papers A & B then transformed it using heat shock into E.Coli. Click to see more.">10</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061119.pdf"><span class="tool" data-tip="Redid the transformations because the transformants overgrew in the kanamycin plates and performed overnight cultures of the PCB302 in E.Coli. Click to see more.">11</span></td>
 +
    <td id="date-pdf"style="color:#36D7FE"><a href="061219.pdf"><span class="tool" data-tip="Performed minipreps on transformations made on 6/10/2019, replated transformations with parts from the registry, redid transformations for PCB302 with one shot and DH5a homemade E. Coli competent cells with kanamycin plates made on 6/11/2019, ran PCR on J23102 promoter sequence and K592009 coding sequence, and created overnight cultures with 3 J23102 promoter ampicillin plates and 1 K592009 coding sequence plate. Click to see more.">12</span></td>
 +
    <td id="date-pdf"style="color:#36D7FE"><a href="061319.pdf"><span class="tool" data-tip="Ran gel electrophoresis on J23102 and K592009, performed a miniprep on the overnight cultures of the transformation samples made on 6/12/2019, performed overnight cultures on the transformations done on 6/12/2019, and performed an analytical restriction digests on blue chromoprotein (K592009) and RFP promotor (J23102), and ran a gel on J23102 and K592009. Click to see more.">13</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061419.pdf"><span class="tool" data-tip="Performed a mini prep on PCB302 plasmid in E. Coli from papers A & B and performed gel extraction on blue chromoprotein and RFP promotor from the analytical digest that was performed on 6/13/2019. Click to see more.">14</span></td>
 +
    <td id="date-pdf">15</td>
 +
      </tr>
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 +
      <tr id="trow">
 +
    <td id="date-pdf">16</td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061719.pdf"><span class="tool" data-tip="Simulated an artificial ligation of blue chromoprotein part into J23102 promoter and pcba1020-r0040 minus RFP on snap gene, ligated BP part with J23102 from the gel extraction, transformed the BP-promoter construct into DH5a chemically competent cells, transformed PCB302 into One Shot Top 10 chemically competent E. Coli cells from papers A&B, and performed restriction digest on PCB302 from previous mini-prepped samples on 6/14/2019 in E. Coli from papers A&B. Click to see more.">17</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061819.pdf"><span class="tool" data-tip="Ran a gel on the restriction digest for the PCB302 samples A &B from 6/17/2019 to verify if we have the correct parts, performed colony PCR on ligations from previous day, made overnight cultures on the ligations as well as the PCB302 plasmid in E. Coli from papers A & B, and created primers for PCB302 partial sequence on snapgene. Click to see more.">18</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="061919.pdf"><span class="tool" data-tip="Ran a gel for colony PCR on BCP K592009 and J23102 promoter ligation and redid overnight cultures on PCB302 in E. Coli from papers A &B and on the BCP K592009 and J23102 promoter ligation. Click to see more">19</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062019.pdf"><span class="tool" data-tip="Performed minipreps on the overnights from the previous day, made glycerol stocks for the overnight cultures from the previous day, and prepared the ASP-8A media. Click to see more.">20</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062119.pdf"><span class="tool" data-tip="Prepared the ASP-8A media, performed restriction digest on the mini prepped ligation samples from 6/20/2019, performed PCR on PCB302 in E. Coli from papers A&B, and ran a gel for the restriction digest on the ligation. Click to see more.">21</span></td>
 +
    <td id="date-pdf">22</td>
 +
      </tr>
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 +
      <tr id="trow">
 +
    <td id="date-pdf">23</td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062419.pdf"><span class="tool" data-tip="Made PII Trace Metal mix, performed gel electrophoresis on PCR samples of PCB302 in E. Coli from papers A & B minipreps done on 6/20/2019, and fed the O. Marina. Click to see more.">24</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062519.pdf"><span class="tool" data-tip="Redid gel electrophoresis on PCR products of K592009 & J23102 ligations and the PCB302 in E. Coli from papers A & B, redid PCR on K592009 & J23102 ligations, performed restriction digest on PCB302 from mini preps in E. Coli from papers A & B from colony on 6/20/2019, made ASP-8A medium, did transformations on K592009 & J23102 ligation mix from 6/17/2019, and fed O. Marina. Click to see more.">25</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062619.pdf"><span class="tool" data-tip="Ran a gel on restriction digest of PCB302 from the miniprep colony 7 in E. Coli from papers A & B from 6/20/2019 and on the PCR of ligations of K592009 and J23102 from 6/25/2019, checked on the algae, filtered some seawater, checked the ligation transformations from 6/25/2019 for growth, and fed the O. Marina. Click to see more.">26</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062719.pdf"><span class="tool" data-tip="Transferred O. Marina and D. Tertiolecta into filtered, autoclaved seawater, filtered and autoclaved more seawater, verified algal life, and fed the O. Marina. Click to see more.">27</span></td>
 +
    <td id="date-pdf" style="color:#36D7FE"><a href="062819.pdf"><span class="tool" data-tip="Ran a PCR on the PCB302 plasmid straight from paper solution A & B and transferred O. Marina, S. Microadiatic, D. Tertiolecta into other cultures and observed the algae. Click to see more.">28</span></td>
 +
    <td id="date-pdf" >29</td>
 +
      </tr>
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      <tr id="trow">
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    <td id="date-pdf">30</td>
 +
    <td id="date-pdf"></td>
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    <td id="date-pdf"></td>
 +
    <td id="date-pdf"></td>
 +
    <td id="date-pdf"></td>
 +
    <td id="date-pdf"></td>
 +
    <td id="date-pdf"></td>
 +
      </tr>
 +
    </tbody> </table>
 +
<!--</div>-->
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</div> </div>
 +
 +
<!--July-->
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<div class="col">
 +
  <div class="container">
 +
<!--<div class="container col-sm-4 col-md-7 col-lg-4 mt-5">-->
 +
<table class="table" cellspacing="0" cellpadding="0" id="table2">
 +
  <tr id="title">
 +
    <th colspan="7" class="text-center" id="header">July 2019</th>
 +
  </tr>
 +
 +
    <tr id="days">
 +
      <th style="color:red">S</th>
 +
      <th>M</th>
 +
      <th>T</th>
 +
      <th>W</th>
 +
      <th>T</th>
 +
      <th>F</th>
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      <th>S</th>
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    </tr>
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  <tbody>
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    <tr id="trow">
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070119.pdf"><span class="tool" data-tip="Transformed new samples of PCB302 (papers 1 & 2) into E. Coli, transformed blue chromoprotein composite, made YM media plate with kanamycin and YM broth, verified algal life, ran gel on PCR of PCB302 in E. Coli from papers A & B from 6/28/2019, and checked concentrations on PCB302 from new samples. Click to see more.">1</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070219.pdf"><span class="tool" data-tip="Made YM media plates with kanamycin, transformed PCB302 plasmid into agrobacterium tumefaciens, made overnight cultures from PCB302 in E. Coli transformations, verified algal life, transformed codon optimized RFP in E. Coli, and performed colony PCR on PCB302 in E. Coli. Click to see more.">2</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070319.pdf"><span class="tool" data-tip="Created new media for the algae, made glycerol stocks, ran a PCR analysis of PCB302 in E. Coli from papers 1 & 2, transformed blue chromoprotein into E. Coli, performed a miniprep on the PCB302 in E. Coli from papers 1 & 2, and fed O. Marina. Click to see more.">3</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070419.pdf"><span class="tool" data-tip="Ran a restriction digest of PCB302 in E. Coli from papers 1 & 2, performed gel electrophoresis analysis of PCB302 in E. Coli papers 1 & 2 digest, made cultures for the S. microadriaticum in L1 and L1+F2, and prepared ASP-8A media with filtered seawater. Click to see more.">4</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070519.pdf"><span class="tool" data-tip="Ran a PCR on PCB302 in E. Coli straight from papers 1 & 2 and made overnight cultures of PCB302 in Agrobacterium Tumefaciens from papers 1 & 2. Click to see more.">5</span></td>
 +
  <td id="date-pdf">6</td>
 +
    </tr>
 +
 +
    <tr id="trow">
 +
  <td id="date-pdf">7</td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070819.pdf"><span class="tool" data-tip="Performed minipreps on the PCB302 in Agrobacterium tumefaciens from papers 1 & 2, made glycerol stocks for overnight PCB302 Agrobacterium cultures papers 1 & 2, and determined algae concentration. Click to see more.">8</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="070919.pdf"><span class="tool" data-tip="Ran gel on PCR products of PCB302-gfp-MBD in E. Coli from papers 1 & 2, transformed codon optimized RFP into E. Coli, transformed blue chromoprotein into E. Coli, performed PCR on PCB302 in A. Tumefaciens from miniprep on 7/8/2019, ran gel on PCR of PCB302 in A. Tumefaciens from miniprep, transformed PCB302 into A. Tumefaciens from papers 1 & 2, and made overnight cultures of PCB302 in A. Tumefaciens from glycerol stocks of minipreps on 7/8/2019. Click to see more.">9</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071019.pdf"><span class="tool" data-tip="Performed PCR on PCB302 from papers 1 & 2, made overnight cultures of codon-optimized-RFP from transformations on 7/9/2019, performed transformations on new blue chromoprotein, and performed gel electrophoresis on the PCB302 PCR from today. Click to see more.">10</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071119.pdf"><span class="tool" data-tip="Performed miniprep on overnight cultures of codon optimized RFP from 7/10/2019, made glycerol stocks on overnight cultures of codon optimized RFP, ran restriction digest on codon optimized RFP mini preps, ran a gel on digested codon optimized RFP, cultured algae in ASP-8A with seawater, and made plates for Symbiodinium culture. Click to see more.">11</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071219.pdf"><span class="tool" data-tip="Picked colonies from PCB302 Agrobacterium transformations from papers 1 & 2, made overnight cultures on PCB302 Agrobacterium transformations, performed minipreps on PCB302 in A. Tumefaciens’ glycerol stocks made on 7/8/2019, ran restriction digest on codon-optimized-RFP, performed colony PCR on blue chromoprotein, and made overnight cultures on PCB302 in A. Tumefaciens transformations from 7/9/2019. Click to see more.">12</span></td>
 +
  <td id="date-pdf">13</td>
 +
    </tr>
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 +
    <tr id="trow">
 +
  <td id="date-pdf">14</td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071519.pdf"><span class="tool" data-tip="Made glycerol stock for overnight cultures of transformations done on 7/9/2019, performed miniprep on PCB302 in A. Tumefaciens’ overnight cultures from 7/9/2019, did transformations on blue chromoprotein and DinoIII part 2, ran gel on blue chromoprotein colony PCR, and made overnight cultures on pGEX-HCG from glycerol stocks. Click to see more.">15</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071619.pdf"><span class="tool" data-tip="Ran PCR on PCB302 from papers 1 & 2 and on PCB302 in A. tumefaciens from miniprep done on 7/15/2019 with new primers, made overnight cultures on PCB302 in A. Tumefaciens’ glycerol stocks made on 7/15/2019, the pGEX-HCG glycerol stocks, and DinoIIIP2, and ran a gel on the miniprep of PCB302 in A. Tumefaciens from 7/15/2019. Click to see more.">16</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071719.pdf"><span class="tool" data-tip="Did minipreps on DinoIII-GFP part 2 and Pgex-HCG, made glycerol stocks for DinoIII-GFP Part 2, did transformations on PCB302 in A. Tumefaciens from papers 1 &2, ran restriction digest on codon-optimized-RFP, and made overnight cultures on blue chromoprotein. Click to see more.">17</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071819.pdf"><span class="tool" data-tip="Did a miniprep on blue chromoprotein overnight cultures and PCB302 in A. Tumefaciens glycerol stocks that were made on 7/15/2019, and did gel electrophoresis on DinoIIIP2 and pGEX-HCG. Click to see more.">18</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="071919.pdf"><span class="tool" data-tip="Made overnight cultures of PCB302 in A. Tumefaciens transformations from 7/17/2019, ran restriction digest on DinoIII-P2 with BgIII and XbaI, and did gel electrophoresis on DinoIII-P2 and the codon optimized RFP digested with BgIII and XbaI. Click to see more.">19</span></td>
 +
  <td id="date-pdf">20</td>
 +
    </tr>
 +
 +
    <tr id="trow">
 +
  <td id="date-pdf">21</td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072219.pdf"><span class="tool" data-tip="Performed miniprep on PCB302 in A. Tumefaciens’ transformations on 7/17/2019, made glycerol stocks of the overnights of PCB302 in A. Tumefaciens from 7/19/2019, ran a gel with BgIII and XbaI on DinoIII P2 and on codon optimized RFP, made overnights of PCB302 in A. Tumefaciens’ glycerol stocks from an unknown date, glycerol stocks of blue chromoprotein from an unknown date, and glycerol stocks of codon-optimized-RFP from an unknown date, and ran restriction digest with new XbaI and BgIII on DinoIII P2 and codon-optimized-RFP. Click to see more.">22</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072319.pdf"><span class="tool" data-tip="Performed miniprep on glycerol stocks of blue chromoprotein from an unknown date and glycerol stocks of codon-optimized-RFP from an unknown date, ran restriction digest on DinoIII P2 and codon-optimized-RFP, performed gel extraction to remove GFP from DinoIII P2 and isolate RFP from codon-optimized-RFP, and made overnights in 100 mL of glycerol stocks of blue chromoprotein from an unknown date, glycerol stocks of codon-optimized-RFP from an unknown date, and glycerol stocks of PCB302 in E. Coli made on 7/3/2019. Click to see more.">23</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072419.pdf"><span class="tool" data-tip="Performed MiDi prep on bluechromoprotein and codon-optimized-RFP, and made YM media. Click to see more.">24</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072519.pdf"><span class="tool" data-tip="Made overnight cultures of glycerol stocks of DinoIII-GFP P2, performed MiDi prep on glycerol stocks of PCB302 in E. Coli made on 7/3/2019, transformed improved mCherry into E. Coli, and sent K1357009 and J23102 for sequencing. Click to see more.">25</span></td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072619.pdf"><span class="tool" data-tip="Performed MiDi prep on DinoIII-GFP P2 and finished the MiDi prep on the glycerol stocks of PCB302 in E. Coli from papers 1 & 2 made on 7/3/2019. Click to see more.">26</td>
 +
  <td id="date-pdf">27</td>
 +
    </tr>
 +
 +
    <tr id="trow">
 +
  <td id="date-pdf">28</td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072919.pdf"><span class="tool" data-tip="Ran a restriction digest on DinoIII-GFP P2 and made overnight cultures for PCB302 in E. Coli glycerol stocks that were made on 7/3/2019. Click to see more.">29</td>
 +
  <td id="date-pdf">30</td>
 +
  <td id="date-pdf" style="color:#36D7FE"><a href="072919.pdf"><span class="tool" data-tip="Performed mini prep on PCB302 in E. Coli overnight cultures made from 7/3/2019, ran restriction digest on the PCB302 miniprep samples from today, on the DinoIII plasmid, and on the codon-optimized-RFP. Click to see more.">31</td>
 +
  <td id="date-pdf" ></td>
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"></td>
 +
    </tr>
 +
 +
  </tbody> </table>
 +
<!--</div>-->
 +
</div> </div>
 +
 +
<!--August-->
 +
<div class="col">
 +
<!--<div class="container col-sm-4 col-md-7 col-lg-4 mt-5">-->
 +
<div class="container">
 +
  <table class="table" cellspacing="0" cellpadding="0" id="table3">
 +
    <tr id="title">
 +
      <th colspan="7" class="text-center" id="header">August 2019</th>
 +
    </tr>
 +
 +
      <tr id="days">
 +
        <th style="color:red">S</th>
 +
        <th>M</th>
 +
        <th>T</th>
 +
        <th>W</th>
 +
        <th>T</th>
 +
        <th>F</th>
 +
        <th>S</th>
 +
      </tr>
 +
  </thead>
 +
  <tbody class="table.table-hover-cells" id="calendar-dates">
 +
  <tr id="trow">
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080119"><span class="tool" data-tip="Performed gel extraction of DinoIII, did transformations on mCherry, did colony PCR on PCB302 using gfp primers, primers 3 and 4, did PCR on the source of DNA for the PCB302 using gfp primers, primers 3 and 4, ran an E-gel on the gel extraction product, and ran a restriction digest on RFP for ligation. Click to see more.">1</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080219"><span class="tool" data-tip="Ran a gel on the PCR products from yesterday, ran a gel and performed gel extraction on the digested codon optimized RFP, did ligation RFP and DinoIII plasmid, and ligation for the composite part. Click to see more.">2</td>
 +
<td id="date-pdf">3</td>
 +
<td id="date-pdf">4</td>
 +
  </tr>
 +
 +
  <tr id="trow" >
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080519"><span class="tool" data-html="true" data-tip="Linearize DinoIII with GFP miniprep, cultured a larger volume of Symbiodinium and Oxyrrhis Marina, and filtered and autoclaved seawater. Click to see more.">5</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080619"><span class="tool" data-tip="Did heat kill on the restriction digested Dino III mini prep samples #1 and #10 from 7/17/2019, performed gel extraction on linearized Dino III with GFP, transformed DinoIII with the codon optimized RFP into E. Coli using OneShot chemically competent cells. Click to see more.">6</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080719"><span class="tool" data-tip="Transformed mCherry into BL21 and NEBa cells, did PCR on the DinoIII-rfp colonies from the transformations that were done on 8/6/2019, ran a restriction digest on codon optimized RFP in a 200 µL reaction, and made overnight cultures on the ligation of DinoIII and RFP. Click to see more.">7</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="080819"><span class="tool" data-tip="Mini prepped DinoIII with RFP from 8/6/2019 transformations, ran a gel on PCR of DinoIII with RFP transformation, performed ethanol precipitation on codon optimized RFP digest from 8/7/2019, made O. Marina cultures, made glycerol stocks of DinoIII-RFP from 8/6/2019 transformations, and sent DinoIII-GFP, Dino-RFP, and codon optimized RFP for sequencing. Click to see more.">8</td>
 +
<td id="date-pdf">9</td>
 +
<td id="date-pdf">10</td>
 +
<td id="date-pdf">11</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081219"><span class="tool" data-tip="Transformed PCB302 into E. Coli from papers A & B, made new algae cultures for O. Marina and S. Microdraticum, transformed mCherry using Rosetta-Gami competent cells, made 500 mL cultures for DinoIII with RFP and GFP, and made overnight cultures for Test Device 1 with positive/negative controls in LB, TB, YM, and YPD. Click to see more.">12</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081319"><span class="tool" data-tip="Did a mass miniprep on DinoIII GFP and Dino RFP 500 mL overnight cultures, did characterization of Test Device 1 and 5 with positive/negative controls in LB, TB, YM, and YPD, did colony PCR on PCB302 A & B, ran a gel on the colony PCR products, made overnight cultures of PCB302 in 250 mL. Click to see more.">13</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081419"><span class="tool" data-tip="Did a mass miniprep on the overnight cultures of PCB302, ran a diagnostic restriction digest on the DinoIII RFP and Dino GFP, ran a gel alongside the undigested mini prep of DinoIII GFP/RFP DNA with diagnostic restriction digest and PCB302 mini preps, made overnight cultures on mcherry transformations, performed colony PCR on the mcherry transformations. Click to see more.">14</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081519"><span class="tool" data-tip="Linearize Dino III plasmids, ran a gel on mCherry PCR, ran a gel on overnights for each plasmid, made overnights for the characterization of Test Device 1, Test Device 5, Positive control, Negative control colony 1 and 2 for each in LB, TB, YM, and YPD media, mini prepped mcherry overnights, made glycerol stocks of mcherry overnights, made glycerol stocks of PCB302 from 8/12/2019, and performed gel extraction and used Whattman paper for parts. Click to see more.">15</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081619"><span class="tool" data-tip="Transformed PCB302 into A. Tumefaciens, performed gel extraction on DinoIII GFP and Dino III RFP, and did an ethanol precipitation on the gel extraction samples. Click to see more.">16</td>
 +
<td id="date-pdf">17</td>
 +
<td id="date-pdf">18</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf" style="color:#36D7FE"><a href="081919"><span class="tool" data-tip="Performed colony PCR on PCB302 in A. Tumefaciens from 8/16/2019, made overnights on PCB302 in A. Tumefaciens from 8/16/2019, did ethanol precipitation on Dino III RFP samples, made overnights for the characterization for Test Device 1, Test Device 5, Positive control, Negative control colony 1 and 2 for each in LB, TB, YM, and YPD media. Click to see more.">19</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082019"><span class="tool" data-tip="Made overnight cultures for the glycerol stocks of mCherry, GFP, RFP, and HCG B3 loop in pGEX, streaked plates of the glycerol stocks of mCherry, GFP, RFP, and HCG B3 loop in pGEX, performed gel extraction on DinoIII-GFP and DinoIII-RFP, and did an ethanol precipitation on the gel extraction. Click to see more.">20</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082119"><span class="tool" data-tip="Finished the ethanol precipitation from 8/20/2019. Click to see more.">21</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082219"><span class="tool" data-tip="Ran a digest on DinoIII-RFP, ran an overnight gel on DinoIII-RFP, and made overnight cultures on PCB302 in A. Tumefaciens from the trial 2 transformation done on 8/16/2019. Click to see more.">22</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082319"><span class="tool" data-tip="Performed gel extraction on DinoIII-RFP. Click to see more.">23</td>
 +
<td id="date-pdf">24</td>
 +
<td id="date-pdf">25</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf">26</td>
 +
<td id="date-pdf">27</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082819"><span class="tool" data-tip="Transformed DinoIII-GFP into O. Marina, transformed the original mCherry, and pelleted and resuspended the PCB302 after the fresh media. Click to see more.">28</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="082919"><span class="tool" data-tip="Transformed the original mCherry using NEB 5 alpha electrocompetent cells. Click to see more.">29</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="083019"><span class="tool" data-tip="Made overnight cultures of the original mCherry part and checked the PCB302 in A. Tumefaciens. Click to see more.">30</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="083119"><span class="tool" data-tip="Made glycerol stocks of mCherry from the transformation done on 8/29/2019. Click to see more.">31</td>
 +
<td id="date-pdf"></td>
 +
  </tr>
 +
  </tbody>
 +
</table>
 +
<!--</div>-->
 +
</div></div>
 +
</div>
 +
 +
<br>
 +
<!--September-->
 +
<div class="row align-items-center">
 +
<div class="col">
 +
<!--<div class="container col-sm-4 col-md-7 col-lg-4 mt-5">-->
 +
<div class="container">
 +
  <table class="table" cellspacing="0" cellpadding="0" id="table4">
 +
    <tr id="title">
 +
      <th colspan="7" class="text-center" id="header">September 2019</th>
 +
    </tr>
 +
 +
      <tr id="days">
 +
        <th style="color:red">S</th>
 +
        <th>M</th>
 +
        <th>T</th>
 +
        <th>W</th>
 +
        <th>T</th>
 +
        <th>F</th>
 +
        <th>S</th>
 +
      </tr>
 +
  </thead>
 +
  <tbody class="table.table-hover-cells" id="calendar-dates">
 +
  <tr id="trow">
 +
<td id="date-pdf">1</td>
 +
<td id="date-pdf">2</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="090319"><span class="tool" data-tip="Made overnight cultures of the original mCherry from the glycerol stocks made on 8/31/2019, checked the PCB302 in A. Tumefaciens OD, ran a digest on DinoIII-RFP for gel extraction, and transformed S. Microadriaticum into DinoGFP and DinoRFP. Click to see more.">3</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="090419"><span class="tool" data-tip="Performed a miniprep on the original mCherry overnights. Click to see more.">4</td>
 +
<td id="date-pdf">5</td>
 +
<td id="date-pdf">6</td>
 +
<td id="date-pdf">7</td>
 +
  </tr>
 +
 +
  <tr id="trow" >
 +
<td id="date-pdf">8</td>
 +
<td id="date-pdf">9</td>
 +
<td id="date-pdf"><a href="091019">10</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="091119"><span class="tool" data-tip="Made overnight cultures of the transformed PCB302 glycerol stocks and did a cell count for the transformed symbiodinium. Click to see more.">11</td>
 +
<td id="date-pdf">12</td>
 +
<td id="date-pdf">13</td>
 +
<td id="date-pdf">14</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf">15</td>
 +
<td id="date-pdf">16</td>
 +
<td id="date-pdf">17</td>
 +
<td id="date-pdf">18</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="091919"><span class="tool" data-tip="Transformed S. Microadriaticum using fungi electroporation protocol. Click to see more.">19</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="092019"><span class="tool" data-tip="Monitored the transformed S. Microadriaticum from 9/19/19. Click to see more.">20</td>
 +
<td id="date-pdf">21</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf">22</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="092319"><span class="tool" data-tip="Experimented the new resuspension methods for Symbiodinium and O. Marina and made the YM and LB plates with kanamycin. Click to see more.">23</td>
 +
<td id="date-pdf">24</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="092519"><span class="tool" data-tip="Transformed PCB302 into A. Tumefaciens and tested the yeast transformation protocol on S. Microadriaticum. Click to see more.">25</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="092619"><span class="tool" data-tip="Transformed PCB302 into A. Tumefaciens and observed the S. Microadriaticum from the transformation. Click to see more.">26</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="092719"><span class="tool" data-tip="Transformed Dino RFP & GFP into S. microadriaticum. Click to see more.">27</td>
 +
<td id="date-pdf">28</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf">29</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="093019"><span class="tool" data-tip="Made overnight cultures of PCB302 in A.Tume transformations from 9/26/19. Click to see more.">30</td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
  </tr>
 +
 +
  </tbody>
 +
</table>
 +
<!--</div>-->
 +
</div> </div>
 +
 +
<!--October-->
 +
<div class="col">
 +
  <div class="container">
 +
<!--<div class="container col-sm-4 col-md-7 col-lg-4 mt-5">-->
 +
<table class="table" cellspacing="0" cellpadding="0" id="table5">
 +
  <tr id="title">
 +
    <th colspan="7" class="text-center" id="header">October 2019</th>
 +
  </tr>
 +
 +
    <tr id="days">
 +
      <th style="color:red">S</th>
 +
      <th>M</th>
 +
      <th>T</th>
 +
      <th>W</th>
 +
      <th>T</th>
 +
      <th>F</th>
 +
      <th>S</th>
 +
    </tr>
 +
  </thead>
 +
 +
  <tbody class="table.table-hover-cells" id="calendar-dates">
 +
  <tr id="trow">
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="100119"><span class="tool" data-tip="Performed colony PCR on the transformed PCB302 in A. Tumefaciens, ran a gel on the PCR products, used the flow cytometer to measure O. Marina and Symbiodinium, and used the microscope to observe the cells. Click to see more.">1</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="100219"><span class="tool" data-tip="Made glycerol stocks of PCB302 into A. Tumefaciens from 9/26/2019, created overnight cultures of PCB302 into A. Tumefaciens from 9/26/2019, and performed blank transformations on S. microadriaticum using the yeast electroporation. Click to see more.">2</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="100319"><span class="tool" data-tip="Performed blank transformation on S. Microadriaticum and O. Marina using Dinoflagellate transformation protocol. Click to see more.">3</td>
 +
<td id="date-pdf"><a href="100419">4</td>
 +
<td id="date-pdf"><a href="100519">5</td>
 +
  </tr>
 +
 +
  <tr id="trow" >
 +
<td id="date-pdf"><a href="100619">6</td>
 +
<td id="date-pdf"><a href="100719">7</td>
 +
<td id="date-pdf"><a href="100819">8</td>
 +
<td id="date-pdf"><a href="100919">9</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="101019"><span class="tool" data-tip="Performed ethanol precipitation on the Dino III RFP gel extraction. Click here to see more.
 +
">10</td>
 +
<td id="date-pdf" style="color:#36D7FE"><a href="101119"><span class="tool" data-tip="Transformed S. Microadriaticum using Agrobacterium Tumefaciens Bead Beating and performed PCR on transformed algae. Click here to see more.">11</td>
 +
<td id="date-pdf"><a href="101219">12</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf"><a href="101319">13</td>
 +
<td id="date-pdf"><a href="101419">14</td>
 +
<td id="date-pdf"><a href="101519">15</td>
 +
<td id="date-pdf"><a href="101619">16</td>
 +
<td id="date-pdf"><a href="101719">17</td>
 +
<td id="date-pdf"><a href="101819">18</td>
 +
<td id="date-pdf"><a href="101919">19</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf"><a href="102019">20</td>
 +
<td id="date-pdf"><a href="102119">21</td>
 +
<td id="date-pdf"><a href="102219">22</td>
 +
<td id="date-pdf"><a href="102319">23</td>
 +
<td id="date-pdf"><a href="102419">24</td>
 +
<td id="date-pdf"><a href="102519">25</td>
 +
<td id="date-pdf"><a href="102619">26</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
<td id="date-pdf"><a href="102719">27</td>
 +
<td id="date-pdf"><a href="102819">28</td>
 +
<td id="date-pdf"><a href="102919">29</td>
 +
<td id="date-pdf"><a href="103019">30</td>
 +
<td id="date-pdf"><a href="103119">31</td>
 +
<td id="date-pdf"></td>
 +
<td id="date-pdf"></td>
 +
  </tr>
 +
 +
</tbody>
 +
</table>
 +
<!--</div>-->
 +
</div> </div>
 +
 +
<!--November-->
 +
<div class="col">
 +
  <div class="container">
 +
<!--<div class="container col-sm-4 col-md-7 col-lg-4 mt-5">-->
 +
<table class="table" cellspacing="0" cellpadding="0" id="table6">
 +
  <tr id="title">
 +
    <th colspan="7" class="text-center" id="header">November 2019</th>
 +
  </tr>
 +
 +
    <tr id="days">
 +
      <th style="color:red">S</th>
 +
      <th>M</th>
 +
      <th>T</th>
 +
      <th>W</th>
 +
      <th>T</th>
 +
      <th>F</th>
 +
      <th>S</th>
 +
    </tr>
 +
  </thead>
 +
 +
  <tbody class="table.table-hover-cells" id="calendar-dates">
 +
  <tr id="trow">
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"></td>
 +
  <td id="date-pdf"><a href="110119">1</td>
 +
  <td id="date-pdf"><a href="110219">2</td>
 +
  </tr>
 +
 +
  <tr id="trow" >
 +
  <td id="date-pdf"><a href="110319">3</td>
 +
  <td id="date-pdf"><a href="110419">4</td>
 +
  <td id="date-pdf"><a href="110519">5</td>
 +
  <td id="date-pdf"><a href="110619">6</td>
 +
  <td id="date-pdf"><a href="110719">7</td>
 +
  <td id="date-pdf"><a href="110819">8</td>
 +
  <td id="date-pdf"><a href="110919">9</td>
 +
  </tr>
 +
 +
  <tr id="trow">
 +
  <td id="date-pdf"><a href="111019">10</td>
 +
  <td id="date-pdf"><a href="111119">11</td>
 +
  <td id="date-pdf"><a href="111219">12</td>
 +
  <td id="date-pdf"><a href="111319">13</td>
 +
  <td id="date-pdf"><a href="111419">14</td>
 +
  <td id="date-pdf"><a href="111519">15</td>
 +
  <td id="date-pdf"><a href="111619">16</td>
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  <td id="date-pdf"><a href="111719">17</td>
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  <td id="date-pdf"><a href="111819">18</td>
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  <td id="date-pdf"><a href="111919">19</td>
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  <td id="date-pdf"><a href="112019">20</td>
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  <td id="date-pdf"><a href="112119">21</td>
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  <td id="date-pdf"><a href="112219">22</td>
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  <td id="date-pdf"><a href="112319">23</td>
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  </tr>
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 +
  <tr id="trow">
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  <td id="date-pdf"><a href="112419">24</td>
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  <td id="date-pdf"><a href="112519">25</td>
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  <td id="date-pdf"><a href="112619">26</td>
 +
  <td id="date-pdf"><a href="112719">27</td>
 +
  <td id="date-pdf"><a href="112819">28</td>
 +
  <td id="date-pdf"><a href="112919">29</td>
 +
  <td id="date-pdf"><a href="113019">30</td>
 +
  </tr>
 +
  </tbody>
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  </table>
 +
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Latest revision as of 20:03, 16 October 2019

GSU iGEM
Notebook
June 2019
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23 24 25 26 27 28 29
30
July 2019
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August 2019
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

September 2019
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
October 2019
S M T W T F S
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
November 2019
S M T W T F S
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

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