Difference between revisions of "Team:Georgia State/Description"
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Revision as of 02:10, 4 October 2019
The Problem
Our team was inspired by the Netflix documentary, Chasing Coral. Coral bleaching, the loss of algal symbionts necessary for the survival of cnidarian reef organisms, is a disastrous environmental issue with global consequences. No single factor has been established as the cause of this catastrophe, but there are a multitude of suspects including increased greenhouse gas emissions and rising seawater temperatures.The Solution
Whatever the cause, we believe a solution may involve genetically modifying the symbiotic microalgae, Symbiodinium, that live within corals.
The Plan
We are establishing both culturing and transformation protocols for these microalgae symbionts. We began by optimizing culturing techniques for Symbiodinium microadriaticum, Oxyrrhis marina (our model organism), and Dunaliella tertiolecta (the food source for O. marina).
Sprecher,B., Zhang,H. & Lin, S. (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821
Ortiz-Matamoros, M.F., Islas-Flores, T., Voigt, B., Menzel, D., Baluška, F. & Villanueva, M.A. (2015, July 13). Heterologous DNA Uptake in Cultured Symbiodinium spp. Aided by Agrobacterium tumefaciens. doi:10.1371/journal. Pone.0132693