Difference between revisions of "Team:Mingdao/Achievement"

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<div class="menuIconBox"><a href="https://2018.igem.org/Competition/Results"><img class="menuIcon" src="https://static.igem.org/mediawiki/2018/8/81/T--Biomarvel--6.achi12.jpg" style="max-width: 400px;"></a></div>
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<h2>Competition Deliverables</h2>
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      <ul class="list-unstyled components">
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<ul>
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        <li>
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<li>All deliverables were met.</li>
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          <a href="https://2018.igem.org/Team:USTC">HOME</a>
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<div id="medalBox1">
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          <a href="#projectSubmenu" data-toggle="collapse" aria-expanded="false">PROJECT <i class="fa fa-caret-down"></i></a>
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<a href="https://2018.igem.org/Team:BioMarvel/Attributions"><h2>Attributions</h2></a>
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          <ul class="collapse list-unstyled" id="projectSubmenu">
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<ul>
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            <li>
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<li>You can check out what our team did and how others helped our project.</li>
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              <a href="https://2018.igem.org/Team:USTC/Description">Description</a>
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<div id="medalBox1">
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              <a href="https://2018.igem.org/Team:USTC/Design">Design</a>
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<a href="https://2019.igem.org/Team:Mingdao/Description"><h2>Project Inspiration and Description</h2></a>
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            </li>
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<ul>
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            <li>
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<li>We successfully completed the writing of Project Inspiration </li>
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              <a href="https://2018.igem.org/Team:USTC/Results">Results</a>
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</ul>
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              <a href="https://2018.igem.org/Team:USTC/Hardware">Hardware</a>
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/d/de/T--Biomarvel--achi_silver.png">
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              <a href="https://2018.igem.org/Team:USTC/Safety">Safety</a>
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<a href="https://2018.igem.org/Team:BioMarvel/WET_LAB/Experiments"><h2>Validated Part</h2></a>
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            </li>
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<ul>
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    <li>
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<li>We submitted BBa_K2885000, BBa_K2885001, and BBa_K2885002.</li>
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              <a href="https://2018.igem.org/Team:USTC/Parts">Parts</a>
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<li>Validation can be found on our result page and registry.</li>
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        <!--Project-->
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<a href="https://2018.igem.org/Team:BioMarvel/Human_Practices"><h2>Human Practices</h2></a>
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          <a href="#modelSubmenu" data-toggle="collapse" aria-expanded="false">MODEL <i class="fa fa-caret-down"></i></a>
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<li>We have investigated related issues and engaged with our relevant communities.</li>
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              <a href="https://2018.igem.org/Team:USTC/Model">Overview</a>
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            </li>
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<a href="https://2018.igem.org/Team:BioMarvel/Collaborations"><h2>Collaboration</h2></a>
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            <li>
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<ul>
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              <a href="https://2018.igem.org/Team:USTC/Model/Single_Cell_Model">Single-cell Model</a>
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<li>We have significantly worked with currently registered 2018 iGEM teams in a meaningful way.</li>
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            </li>
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</ul>
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            <li>
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</div>
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              <a href="https://2018.igem.org/Team:USTC/Model/System_Analysis">System Analysis</a>
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<section id="mSection">
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            <li>
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/4/4a/T--Biomarvel--achi_gold.png">
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              <a href="https://2018.igem.org/Team:USTC/Model/Performance_evaluation">Performance Evaluation</a>
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<div id="medalBox3">
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<a href="https://2018.igem.org/Team:BioMarvel/Human_Practices"><h2>Integrated Human Practices</h2></a>
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    <li>
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<ul>
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              <a href="https://2018.igem.org/Team:USTC/Model/Parameters">Parameters</a>
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<li>We expanded on our silver medal activity by demonstrating how we have integrated the investigated issues into our project. Integrated human practices helped to improve our design and execution of the project.</li>
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            </li>
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          </ul>
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        <!--Model-->
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<a href="https://2018.igem.org/Team:BioMarvel/Demonstrate"><h2>Demonstrate</h2></a>
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        <li>
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<ul>
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          <a href="https://2018.igem.org/Team:USTC/InterLab">INTERLAB</a>
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<li>We demonstrated that our GBP-ProG works well and can be applied to a novel platform for heart failure patients.</li>
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        </li>
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</ul>
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        <!--Interlab-->
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          <a href="https://2018.igem.org/Team:USTC/Notebook">NOTEBOOK</a>
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/d/d8/T--Biomarvel--achi_special.png">
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        <!--Notebook-->
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        <li>
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<a href="https://2018.igem.org/Team:BioMarvel/Entrepreneurship"><h2>Entrepreneurship</h2></a>
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          <a href="#HPSubmenu" data-toggle="collapse" aria-expanded="false">HUMAN PRACTICES <i class="fa fa-caret-down"></i></a>
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<li>We built projects and focused on commercializing our work. Check out the entrepreneurship page.</li>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices">Overview</a>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices/General_HP">General HP</a>
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              <a href="https://2018.igem.org/Team:USTC/Human_Practices/Integrated_HP">Integrated HP</a>
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    <li>
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      <a href="https://2018.igem.org/Team:USTC/Collaborations">Collaborations</a>
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<!--Achievements-->
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          <a href="https://2018.igem.org/Team:USTC/Achievements">ACHIEVEMENTS</a>
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          <a href="#Teamsubmenu" data-toggle="collapse" aria-expanded="false">TEAM <i class="fa fa-caret-down"></i></a>
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              <a href="https://2018.igem.org/Team:USTC/Team">Members</a>
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              <a href="https://2018.igem.org/Team:USTC/Attributions">Attributions</a>
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                                <a class="nav-link" href="#section1">Bronze</a>
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                                <a class="nav-link" href="#section2">Silver</a>
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                                <a class="nav-link" href="#section3">Gold</a>
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            <div class="container">
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<div class="card mb-3 border-light">
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                    <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/7/7e/T--USTC--Achievementstitle.png" alt="">
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                        <h4 class="card-title">Bronze:</h4>
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                                    <th>Number</th>
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                                    <th>Explanation</th>
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                                    <th>Contents</th>
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                                    <td scope="row">1</td>
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                                    <td>Registration and Jamboree Attendance</td>
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                                    <td>We tried our best to realize our project during the summer holiday with great enthusiasm. We also come to attend the Giant Jamboree, sharing our project and learning from other teams’ great ideas!</td>
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                                    <td scope="row">2</td>
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                                    <td>Competition Deliverables</td>
 +
                                    <td>We have filled out all the forms and pages that are required carefully. Also, we prepared our poster and presentation to show our project to everyone else.</td>
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                                <tr>
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                                    <td scope="row">3</td>
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                                    <td>Attributions</td>
 +
                                    <td>Here is the link for attributions: <a class="card-link" href="https://2018.igem.org/Team:USTC/Attributions">https://2018.igem.org/Team:USTC/Attributions</a>. We sincerely thank those people whom had given so much support to us.</td>
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                                    <td scope="row">4</td>
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                                    <td>Characterization/Contribution</td>
 +
                                    <td>We successfully participated in the 2018 InterLab Study and our result had been accepted by iGEM Foundation. Here is the link: <a class="card-link" href="https://2018.igem.org/Team:USTC/Attributions">https://2018.igem.org/Team:USTC/Interlab</a></td>
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                    <div class="card-body" id="section2">
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                        <h4 class="card-title">Silver:</h4>
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                                    <th>Number</th>
 +
                                    <th>Explanation</th>
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                                    <th>Contents</th>
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                                    <td scope="row">1</td>
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                                    <td>Validated Part</td>
 +
                                    <td>This year we use quorum sensing genes from distribution kit to construct JTPI (BBa_K2768010), which can activate expression of genes downstream. And we also added a positive feedback to make it able to regulate in low AHL concentration. We have proved that this part can activate the expression downstream with positive feedback. You can check it here: <a class="card-link" href="http://parts.igem.org/Part:BBa_K2768010">http://parts.igem.org/Part:BBa_K2768010</a></td>
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                                    <td scope="row">2</td>
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                                    <td>Collaboration</td>
 +
                                    <td>We communicated with Team: Fudan, Nanjing-China, XJTU-China, Jilin-China, BNU-China about our projects, and we provided Team: HUST_China with experimental materials they needed. We helped Team: USTC-Software to test their Software and gave them some useful advice. Team Peking helped us in our model. We participated in The 5th Conference of China iGEMer Community and The 6th iGEM Asia-Parcific Conference to show our project and communicate with other teams.</td>
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                                    <td scope="row">3</td>
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                                    <td>Human Practice</td>
 +
                                    <td>(1) We have been to three tobacco factories (Hefei Cigarette Factory, Zhangzhou Cigarette Factory and Wuhu Cigarette Factory) to research our project. We knew a lot about tobacco and tobacco waste, and we had a fresh understanding of pathway of producing the prodrug. (2) For the purpose of collecting the community’s views about tobacco and propagating iGEM synthetic biology, we designed a questionnaire and have collected hundreds of results. (3) In the “Science Open Weekend”, we opened the lab for thousands of people to visit. (4) We finished a lecture in the high school to publicize iGEM and synthetic biology knowledge. 5. We actively communicated with local high schools and gave them help of founding new iGEM high school teams. 6. We attended the Asia-Pacific iGEM Conference and Central China iGEM Consortium with iGEMers all over the world. 7. Communication and cooperation with some iGEM teams.</td>
 +
                                </tr>
 +
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                    <div class="card-body" id="section3">
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                        <h4 class="card-title">Gold:</h4>
 +
                        <hr>
 +
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 +
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 +
                                    <th>Number</th>
 +
                                    <th>Explanation</th>
 +
                                    <th>Contents</th>
 +
                                </tr>
 +
                            </thead>
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                            <tbody>
 +
                                <tr>
 +
                                    <td scope="row">1</td>
 +
                                    <td>Integrated Human Practices</td>
 +
                                    <td>During the visit to the 3 tobacco factories, we have learnt a lot about tobacco industry. We considered to use pure nicotine or nicotine in environment to produce 3-succionylpyridine before, but after the visit, we decided to use tobacco waste as our raw material. Our human practices helped us a lot in our project, making our project more close to real industrial production, and we have designed our whole system based on our human practices.</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td scope="row">2</td>
 +
                                    <td>Improve a Previous Part</td>
 +
                                    <td>We added SsrA tag on the C terminus of Part: E0040, making it a new GFP with fast degradation rate (K2768004)</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td scope="row">3</td>
 +
                                    <td>Model Your Project</td>
 +
                                    <td>(1) Single-cell model is consisted of 22 nonlinear ordinary differential equations. We use Matlab solvers to simulate the change of species concentration along the time. Then we analyze the condition of initial steady state and nicotine sensing state with defined parameters. These results strongly prove the feasibility of our design. (2) We analyze the impact of different combinations of promoter strengths and copy numbers to the initial steady states and outputs with nicotine input. Our simulation results present the tendency of initial state change and the signal shifting. According to these phenomena, we can choose the appropriate promoter combinations and plasmid backbones for the realization of our design. (3) In simulation, we change the value of promoter strength and nicotine input. From the simulation results, we acquire the best time for sampling, signal measurement and product collection. At last, the recycle rate (production rate) is calculated to prove the efficiency of manufacturing.</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td scope="row">4</td>
 +
                                    <td>Demonstration of Your Work</td>
 +
                                    <td>We completed most of our design this year. In Sensing System, we selected the most suitable RBS and promotor for our sensing system under the guidance of modeling groups. We successfully constructed the plasmids and confirmed the transformation. In Regulation System, we constructed the plasmids we needed and plotted RFI curve and growth curve. According to the curves, we have proved our Regulation System is worked. In Degradation System, we constructed plasmids containing the genes of three enzymes separately and together. After confirming transformation, we successfully expressed three enzymes separately and together. In summary, it can be safely concluded that our project work! </td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                </div>
 +
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 +
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Revision as of 12:51, 28 September 2019

Bronze:

Number Explanation Contents
1 Registration and Jamboree Attendance We tried our best to realize our project during the summer holiday with great enthusiasm. We also come to attend the Giant Jamboree, sharing our project and learning from other teams’ great ideas!
2 Competition Deliverables We have filled out all the forms and pages that are required carefully. Also, we prepared our poster and presentation to show our project to everyone else.
3 Attributions Here is the link for attributions: https://2018.igem.org/Team:USTC/Attributions. We sincerely thank those people whom had given so much support to us.
4 Characterization/Contribution We successfully participated in the 2018 InterLab Study and our result had been accepted by iGEM Foundation. Here is the link: https://2018.igem.org/Team:USTC/Interlab

Silver:

Number Explanation Contents
1 Validated Part This year we use quorum sensing genes from distribution kit to construct JTPI (BBa_K2768010), which can activate expression of genes downstream. And we also added a positive feedback to make it able to regulate in low AHL concentration. We have proved that this part can activate the expression downstream with positive feedback. You can check it here: http://parts.igem.org/Part:BBa_K2768010
2 Collaboration We communicated with Team: Fudan, Nanjing-China, XJTU-China, Jilin-China, BNU-China about our projects, and we provided Team: HUST_China with experimental materials they needed. We helped Team: USTC-Software to test their Software and gave them some useful advice. Team Peking helped us in our model. We participated in The 5th Conference of China iGEMer Community and The 6th iGEM Asia-Parcific Conference to show our project and communicate with other teams.
3 Human Practice (1) We have been to three tobacco factories (Hefei Cigarette Factory, Zhangzhou Cigarette Factory and Wuhu Cigarette Factory) to research our project. We knew a lot about tobacco and tobacco waste, and we had a fresh understanding of pathway of producing the prodrug. (2) For the purpose of collecting the community’s views about tobacco and propagating iGEM synthetic biology, we designed a questionnaire and have collected hundreds of results. (3) In the “Science Open Weekend”, we opened the lab for thousands of people to visit. (4) We finished a lecture in the high school to publicize iGEM and synthetic biology knowledge. 5. We actively communicated with local high schools and gave them help of founding new iGEM high school teams. 6. We attended the Asia-Pacific iGEM Conference and Central China iGEM Consortium with iGEMers all over the world. 7. Communication and cooperation with some iGEM teams.

Gold:

Number Explanation Contents
1 Integrated Human Practices During the visit to the 3 tobacco factories, we have learnt a lot about tobacco industry. We considered to use pure nicotine or nicotine in environment to produce 3-succionylpyridine before, but after the visit, we decided to use tobacco waste as our raw material. Our human practices helped us a lot in our project, making our project more close to real industrial production, and we have designed our whole system based on our human practices.
2 Improve a Previous Part We added SsrA tag on the C terminus of Part: E0040, making it a new GFP with fast degradation rate (K2768004)
3 Model Your Project (1) Single-cell model is consisted of 22 nonlinear ordinary differential equations. We use Matlab solvers to simulate the change of species concentration along the time. Then we analyze the condition of initial steady state and nicotine sensing state with defined parameters. These results strongly prove the feasibility of our design. (2) We analyze the impact of different combinations of promoter strengths and copy numbers to the initial steady states and outputs with nicotine input. Our simulation results present the tendency of initial state change and the signal shifting. According to these phenomena, we can choose the appropriate promoter combinations and plasmid backbones for the realization of our design. (3) In simulation, we change the value of promoter strength and nicotine input. From the simulation results, we acquire the best time for sampling, signal measurement and product collection. At last, the recycle rate (production rate) is calculated to prove the efficiency of manufacturing.
4 Demonstration of Your Work We completed most of our design this year. In Sensing System, we selected the most suitable RBS and promotor for our sensing system under the guidance of modeling groups. We successfully constructed the plasmids and confirmed the transformation. In Regulation System, we constructed the plasmids we needed and plotted RFI curve and growth curve. According to the curves, we have proved our Regulation System is worked. In Degradation System, we constructed plasmids containing the genes of three enzymes separately and together. After confirming transformation, we successfully expressed three enzymes separately and together. In summary, it can be safely concluded that our project work!