Difference between revisions of "Team:HK SSC"

(Undo revision 162298 by Justinyhchoi (talk))
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    <nav class="navbar navbar-expand beta-menu navbar-dropdown align-items-center navbar-fixed-top navbar-toggleable-sm">
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        <button class="navbar-toggler navbar-toggler-right" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
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                <span class="navbar-caption-wrap"><a class="navbar-caption text-white display-4" href="https://mobirise.co">
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                        HK_SSC</a></span>
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                    <a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Home</a>
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                    <a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Team<br></a>
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                        Project<br></a></li><li class="nav-item"><a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Human Practice<br></a></li><li class="nav-item"><a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Parts<br></a></li><li class="nav-item"><a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Collaboration<br></a></li><li class="nav-item"><a class="nav-link link text-white display-7" href="https://mobirise.co">
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                        Notebook<br></a></li></ul>
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* Docs to Markdown version 1.0β17
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<section class="engine"><a href="https://mobirise.info/s">bootstrap themes</a></section><section class="header1 cid-rAVtEy3FNI" id="header16-1">
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* Wed Sep 25 2019 09:54:22 GMT-0700 (PDT)
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* Source doc: https://docs.google.com/open?id=1cax56EvETOwJh0Be8TEAk4eDMd43HW1cMOFAlzyRcpw
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* This is a partial selection. Check to make sure intra-doc links work.
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<p>Project description
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</p>
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<h3>Abstract/ Overview</h3>
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<p>
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Microcystis aeruginosa is one of the most common cyanobacteria responsible for
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    <div class="container">
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harmful algal blooms. This cyanobacterium produces microcystin, a hepatotoxin
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        <div class="row justify-content-md-center">
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that damages the liver. However, direct lysis of Microcystis aeruginosa may not
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            <div class="col-md-10 align-center">
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best for the environment as it holds ecological values of heavy metal sorption
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                <h1 class="mbr-section-title mbr-bold pb-3 mbr-fonts-style display-1">
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and oxygen synthesis. In this project, we hope to silence the microcystin
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                    ABSTRACT</h1>
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biosynthesis cluster(mcy) using a catalytically dead Cas9 (dCas9) enzyme lacking
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endonuclease activity. When the dCas9 enzyme is co-expressed with a guide
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                <p class="mbr-text pb-3 mbr-fonts-style display-5">
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RNA(sgRNA), the dCas9-sgRNA complex specifically binds to the McyB gene and
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blocks transcript elongation, leading to the repression of the McyB gene without
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                </p>
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altering the chromosome of the Microcystis. Here we provide the design of a
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dCas9-sgRNA expression gene in a shuttle vector that can replicate in both
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E.coli and cyanobacteria. We will also be conducting downstream analysis to see
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how our dCas9-sgRNA expression plasmid affects the microcystin-production rate
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and oxygen synthesis rate of Microcystis.
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                <h1 class="mbr-section-title mbr-bold pb-3 mbr-fonts-style display-1">
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                    Project Inspiration</h1>
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                <p class="mbr-text pb-3 mbr-fonts-style display-5">
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                    Our project is inspired from various aspects, including school lessons, books, news articles hiking trips, and even previous iGEM teams.
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                </p>
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            </div>
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Revision as of 06:12, 26 September 2019

<!DOCTYPE html> Home

bootstrap themes

ABSTRACT

Project Inspiration

Our project is inspired from various aspects, including school lessons, books, news articles hiking trips, and even previous iGEM teams.